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Protein

Ubiquitin-conjugating enzyme E2-24 kDa

Gene

Ubc2

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotation

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei170Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin-protein transferase activity Source: FlyBase

GO - Biological processi

  • lipid storage Source: FlyBase
  • protein polyubiquitination Source: FlyBase
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-DME-1169408. ISG15 antiviral mechanism.
R-DME-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-DME-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174154. APC/C:Cdc20 mediated degradation of Securin.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-DME-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-DME-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-DME-176412. Phosphorylation of the APC/C.
R-DME-179409. APC-Cdc20 mediated degradation of Nek2A.
R-DME-2467813. Separation of Sister Chromatids.
R-DME-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-DME-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
SignaLinkiP52485.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2-24 kDa (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 2
Ubiquitin carrier protein
Ubiquitin-protein ligase
Gene namesi
Name:Ubc2Imported
Synonyms:UbcD2
ORF Names:CG6720Imported
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0015320. Ubc2.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825201 – 232Ubiquitin-conjugating enzyme E2-24 kDaAdd BLAST232

Proteomic databases

PaxDbiP52485.
PRIDEiP52485.

Expressioni

Gene expression databases

BgeeiFBgn0015320.
ExpressionAtlasiP52485. baseline.
GenevisibleiP52485. DM.

Interactioni

Protein-protein interaction databases

BioGridi60561. 16 interactors.
DIPiDIP-17329N.
IntActiP52485. 5 interactors.
MINTiMINT-334725.
STRINGi7227.FBpp0305676.

Structurei

3D structure databases

ProteinModelPortaliP52485.
SMRiP52485.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
InParanoidiP52485.
KOiK20217.
OMAiNICEWRS.
OrthoDBiEOG091G0GF8.
PhylomeDBiP52485.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52485-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSTPAAGSA AEVATSSATS NAPSAPSTTA SNVSNTSQPT TAGTPQARGG
60 70 80 90 100
RGSNANGGAS GSNAGGGDEP RKEAKTTPRI SRALGTSAKR IQKELAEITL
110 120 130 140 150
DPPPNCSAGP KGDNLYEWVS TILGPPGSVY EGGVFFLDIH FSPEYPFKPP
160 170 180 190 200
KVTFRTRIYH CNINSQGVIC LDILKDNWSP ALTISKVLLS ICSLLTDCNP
210 220 230
ADPLVGSIAT QYLQNREEHD RIARLWTKRY AT
Length:232
Mass (Da):24,435
Last modified:October 1, 1996 - v1
Checksum:iDE71AFD8EB618C86
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92663 mRNA. Translation: CAA63351.1.
AE014134 Genomic DNA. Translation: AAF53008.1.
RefSeqiNP_001260362.1. NM_001273433.1.
NP_001285814.1. NM_001298885.1.
NP_477137.1. NM_057789.4.
NP_723616.1. NM_164943.2.
UniGeneiDm.6395.

Genome annotation databases

EnsemblMetazoaiFBtr0080115; FBpp0079704; FBgn0015320.
FBtr0080116; FBpp0079705; FBgn0015320.
FBtr0333492; FBpp0305676; FBgn0015320.
FBtr0345437; FBpp0311562; FBgn0015320.
GeneIDi34487.
KEGGidme:Dmel_CG6720.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X92663 mRNA. Translation: CAA63351.1.
AE014134 Genomic DNA. Translation: AAF53008.1.
RefSeqiNP_001260362.1. NM_001273433.1.
NP_001285814.1. NM_001298885.1.
NP_477137.1. NM_057789.4.
NP_723616.1. NM_164943.2.
UniGeneiDm.6395.

3D structure databases

ProteinModelPortaliP52485.
SMRiP52485.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60561. 16 interactors.
DIPiDIP-17329N.
IntActiP52485. 5 interactors.
MINTiMINT-334725.
STRINGi7227.FBpp0305676.

Proteomic databases

PaxDbiP52485.
PRIDEiP52485.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0080115; FBpp0079704; FBgn0015320.
FBtr0080116; FBpp0079705; FBgn0015320.
FBtr0333492; FBpp0305676; FBgn0015320.
FBtr0345437; FBpp0311562; FBgn0015320.
GeneIDi34487.
KEGGidme:Dmel_CG6720.

Organism-specific databases

CTDi34487.
FlyBaseiFBgn0015320. Ubc2.

Phylogenomic databases

eggNOGiKOG0417. Eukaryota.
COG5078. LUCA.
InParanoidiP52485.
KOiK20217.
OMAiNICEWRS.
OrthoDBiEOG091G0GF8.
PhylomeDBiP52485.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-DME-1169408. ISG15 antiviral mechanism.
R-DME-141430. Inactivation of APC/C via direct inhibition of the APC/C complex.
R-DME-174048. APC/C:Cdc20 mediated degradation of Cyclin B.
R-DME-174084. Autodegradation of Cdh1 by Cdh1:APC/C.
R-DME-174154. APC/C:Cdc20 mediated degradation of Securin.
R-DME-174178. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
R-DME-174184. Cdc20:Phospho-APC/C mediated degradation of Cyclin A.
R-DME-176407. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
R-DME-176408. Regulation of APC/C activators between G1/S and early anaphase.
R-DME-176409. APC/C:Cdc20 mediated degradation of mitotic proteins.
R-DME-176412. Phosphorylation of the APC/C.
R-DME-179409. APC-Cdc20 mediated degradation of Nek2A.
R-DME-2467813. Separation of Sister Chromatids.
R-DME-2559582. Senescence-Associated Secretory Phenotype (SASP).
R-DME-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
SignaLinkiP52485.

Miscellaneous databases

ChiTaRSiUbcD2. fly.
GenomeRNAii34487.
PROiP52485.

Gene expression databases

BgeeiFBgn0015320.
ExpressionAtlasiP52485. baseline.
GenevisibleiP52485. DM.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBCD2_DROME
AccessioniPrimary (citable) accession number: P52485
Secondary accession number(s): Q9VKQ4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 131 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.