P52483 (UB2E3_MOUSE) Reviewed, UniProtKB/Swiss-Prot
Last modified
May 29, 2013.
Version 111.
History...
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize orderNames and origin
| Protein names | Recommended name: Ubiquitin-conjugating enzyme E2 E3 EC=6.3.2.19 Alternative name(s): UbcM2 Ubiquitin carrier protein E3 Ubiquitin-conjugating enzyme E2-23 kDa Ubiquitin-protein ligase E3 | ||||
| Gene names |
| ||||
| Organism | Mus musculus (Mouse) [Reference proteome] | ||||
| Taxonomic identifier | 10090 [NCBI] | ||||
| Taxonomic lineage | Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus![]() |
Protein attributes
| Sequence length | 207 AA. |
| Sequence status | Complete. |
| Protein existence | Evidence at protein level |
General annotation (Comments)
| Function | Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. In vitro catalyzes 'Lys-11'- and 'Lys-48'-, as well as 'Lys-63'-linked polyubiquitination By similarity. Participates in the regulation of transepithelial sodium transport in renal cells. May be involved in cell growth arrest. Ref.7 |
| Catalytic activity | ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine. |
| Pathway | |
| Subunit structure | Interacts with NEDD4L. The ubiquitin-loaded form interacts specifically with importin-11 (IPO11), leading to its import into the nucleus. Ref.5 Ref.6 Ref.7 |
| Subcellular location | Nucleus. Cytoplasm. Note: Shuttles between the nucleus and cytoplasm in a IPO11-dependent manner. Ref.5 Ref.6 |
| Sequence similarities | Belongs to the ubiquitin-conjugating enzyme family. |
Ontologies
Sequence annotation (Features)
| Feature key | Position(s) | Length | Description | Graphical view | Feature identifier | ||||
Molecule processing | |||||||||
|---|---|---|---|---|---|---|---|---|---|
| Chain | 1 – 207 | 207 | Ubiquitin-conjugating enzyme E2 E3 | PRO_0000082475 | |||||
Sites | |||||||||
| Active site | 145 | 1 | Glycyl thioester intermediate | ||||||
Amino acid modifications | |||||||||
| Modified residue | 8 | 1 | Phosphoserine By similarity | ||||||
| Modified residue | 91 | 1 | Phosphotyrosine Ref.8 | ||||||
Experimental info | |||||||||
| Mutagenesis | 145 | 1 | C → S or A: Loss of enzymatic activity, interaction with IPO11, nuclear import, and effect on cell growth. Ref.2 Ref.6 Ref.7 | ||||||
| Sequence conflict | 31 | 1 | E → K in CAA63352. Ref.1 | ||||||
Sequences
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References
| « Hide 'large scale' references | |
| [1] | "Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions." Matuschewski K., Hauser H.P., Treier M., Jentsch S. J. Biol. Chem. 271:2789-2794(1996) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. |
| [2] | "Isolation of growth suppressors from a cDNA expression library." Pestov D.G., Grzeszkiewicz T.M., Lau L.F. Oncogene 17:3187-3197(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], MUTAGENESIS OF CYS-145. Strain: Swiss. |
| [3] | "The transcriptional landscape of the mammalian genome." Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J. Hayashizaki Y.Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: C57BL/6J and DBA/2. |
| [4] | "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Liver. |
| [5] | "Importin-11, a nuclear import receptor for the ubiquitin-conjugating enzyme, UbcM2." Plafker S.M., Macara I.G. EMBO J. 19:5502-5513(2000) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IPO11, SUBCELLULAR LOCATION. |
| [6] | "Ubiquitin charging of human class III ubiquitin-conjugating enzymes triggers their nuclear import." Plafker S.M., Plafker K.S., Weissman A.M., Macara I.G. J. Cell Biol. 167:649-659(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH IPO11, SUBCELLULAR LOCATION, MUTAGENESIS OF CYS-145. |
| [7] | "Participation of the ubiquitin-conjugating enzyme UBE2E3 in Nedd4-2-dependent regulation of the epithelial Na+ channel." Debonneville C., Staub O. Mol. Cell. Biol. 24:2397-2409(2004) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH NEDD4L, MUTAGENESIS OF CYS-145, FUNCTION. |
| [8] | "Quantitative time-resolved phosphoproteomic analysis of mast cell signaling." Cao L., Yu K., Banh C., Nguyen V., Ritz A., Raphael B.J., Kawakami Y., Kawakami T., Salomon A.R. J. Immunol. 179:5864-5876(2007) [PubMed] [Europe PMC] [Abstract] Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT TYR-91, MASS SPECTROMETRY. Tissue: Mast cell. |
| + | Additional computationally mapped references. |
Cross-references
Sequence databases | |
|---|---|
| EMBL GenBank DDBJ | X92664 mRNA. Translation: CAA63352.1. AF003346 mRNA. Translation: AAB60948.1. AK076011 mRNA. Translation: BAC36118.1. AK168072 mRNA. Translation: BAE40046.1. BC011477 mRNA. Translation: AAH11477.1. |
| IPI | IPI00323176. |
| RefSeq | NP_033480.1. NM_009454.2. |
| UniGene | Mm.1485. Mm.393087. |
3D structure databases | |
| ProteinModelPortal | P52483. |
| ModBase | Search... |
Protein-protein interaction databases | |
| IntAct | P52483. 1 interaction. |
PTM databases | |
| PhosphoSite | P52483. |
Proteomic databases | |
| PaxDb | P52483. |
| PRIDE | P52483. |
Protocols and materials databases | |
| StructuralBiologyKnowledgebase | Search... |
Genome annotation databases | |
| Ensembl | ENSMUST00000028398; ENSMUSP00000028398; ENSMUSG00000027011. ENSMUST00000121433; ENSMUSP00000113463; ENSMUSG00000027011. |
| GeneID | 22193. |
| KEGG | mmu:22193. |
| UCSC | uc008kgm.1. mouse. |
Organism-specific databases | |
| CTD | 10477. |
| MGI | MGI:107412. Ube2e3. |
Phylogenomic databases | |
| eggNOG | COG5078. |
| GeneTree | ENSGT00690000102027. |
| HOGENOM | HOG000233455. |
| HOVERGEN | HBG063308. |
| InParanoid | P52483. |
| KO | K06689. |
| OMA | AKPNPKM. |
Enzyme and pathway databases | |
| UniPathway | UPA00143. |
Gene expression databases | |
| ArrayExpress | P52483. |
| Bgee | P52483. |
| Genevestigator | P52483. |
| GermOnline | ENSMUSG00000027011. Mus musculus. |
Family and domain databases | |
| Gene3D | 3.10.110.10. 1 hit. |
| InterPro | IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view] |
| Pfam | PF00179. UQ_con. 1 hit. [Graphical view] |
| SUPFAM | SSF54495. UBQ-conjugat/RWD-like. 1 hit. |
| PROSITE | PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view] |
| ProtoNet | Search... |
Other | |
| NextBio | 302169. |
| SOURCE | Search... |
Entry information
| Entry name | UB2E3_MOUSE | ||||||||
| Accession | Primary (citable) accession number: P52483 Secondary accession number(s): O09180, Q3TI00, Q91X63 | ||||||||
| Entry history |
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| Entry status | Reviewed (UniProtKB/Swiss-Prot) | ||||||||
| Annotation program | Chordata Protein Annotation Program | ||||||||
Relevant documents
| MGD cross-references Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot |
| PATHWAY comments Index of metabolic and biosynthesis pathways |
| SIMILARITY comments Index of protein domains and families |

Clusters with
