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P52482

- UB2E1_MOUSE

UniProt

P52482 - UB2E1_MOUSE

Protein

Ubiquitin-conjugating enzyme E2 E1

Gene

Ube2e1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 121 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination By similarity.By similarity

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei131 – 1311Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. ISG15 ligase activity Source: Ensembl
    3. ubiquitin-protein transferase activity Source: UniProtKB

    GO - Biological processi

    1. histone H2B ubiquitination Source: Ensembl
    2. histone monoubiquitination Source: Ensembl
    3. ISG15-protein conjugation Source: Ensembl
    4. protein K48-linked ubiquitination Source: UniProtKB
    5. protein ubiquitination Source: MGI

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_198533. ISG15 antiviral mechanism.
    REACT_205250. Phosphorylation of the APC/C.
    REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
    REACT_207679. Separation of Sister Chromatids.
    REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 E1 (EC:6.3.2.19)
    Alternative name(s):
    UbcM3
    Ubiquitin carrier protein E1
    Ubiquitin-protein ligase E1
    Gene namesi
    Name:Ube2e1
    Synonyms:Ubce5, Ubcm3
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 14

    Organism-specific databases

    MGIiMGI:107411. Ube2e1.

    Subcellular locationi

    Nucleus By similarity

    GO - Cellular componenti

    1. nucleus Source: UniProtKB
    2. ubiquitin ligase complex Source: Ensembl

    Keywords - Cellular componenti

    Nucleus

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 193192Ubiquitin-conjugating enzyme E2 E1PRO_0000082471Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei2 – 21N-acetylserineBy similarity
    Cross-linki136 – 136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15)By similarity

    Post-translational modificationi

    ISGylation suppresses ubiquitin E2 enzyme activity.By similarity

    Keywords - PTMi

    Acetylation, Isopeptide bond, Ubl conjugation

    Proteomic databases

    PaxDbiP52482.
    PRIDEiP52482.

    PTM databases

    PhosphoSiteiP52482.

    Expressioni

    Gene expression databases

    ArrayExpressiP52482.
    BgeeiP52482.
    GenevestigatoriP52482.

    Interactioni

    Subunit structurei

    Interacts with RNF14.

    Protein-protein interaction databases

    BioGridi204406. 3 interactions.
    IntActiP52482. 1 interaction.
    STRINGi10090.ENSMUSP00000022296.

    Structurei

    3D structure databases

    ProteinModelPortaliP52482.
    SMRiP52482. Positions 46-193.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Compositional bias

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Compositional biasi9 – 1810Poly-Ser

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    HOGENOMiHOG000233455.
    HOVERGENiHBG063308.
    InParanoidiP52482.
    KOiK06689.
    OMAiSIGTQYM.
    OrthoDBiEOG7PCJGX.
    PhylomeDBiP52482.
    TreeFamiTF101117.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52482-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSDDDSRAST SSSSSSSSNQ QTEKEGSTPK KKESKVSMSK NSKLLSTSAK    50
    RIQKELADIT LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI 100
    TFTPEYPFKP PKVTFRTRIY HCNINSQGVI CLDILKDNWS PALTISKVLL 150
    SICSLLTDCN PADPLVGSIA TQYMTNRAEH DRMARQWTKR YAT 193
    Length:193
    Mass (Da):21,333
    Last modified:October 1, 1996 - v1
    Checksum:iB535B3095EF6C445
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92665 mRNA. Translation: CAA63353.1.
    BC003781 mRNA. Translation: AAH03781.1.
    CCDSiCCDS26837.1.
    RefSeqiNP_033481.1. NM_009455.3.
    UniGeneiMm.4429.

    Genome annotation databases

    EnsembliENSMUST00000022296; ENSMUSP00000022296; ENSMUSG00000021774.
    GeneIDi22194.
    KEGGimmu:22194.
    UCSCiuc007shu.1. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X92665 mRNA. Translation: CAA63353.1 .
    BC003781 mRNA. Translation: AAH03781.1 .
    CCDSi CCDS26837.1.
    RefSeqi NP_033481.1. NM_009455.3.
    UniGenei Mm.4429.

    3D structure databases

    ProteinModelPortali P52482.
    SMRi P52482. Positions 46-193.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 204406. 3 interactions.
    IntActi P52482. 1 interaction.
    STRINGi 10090.ENSMUSP00000022296.

    PTM databases

    PhosphoSitei P52482.

    Proteomic databases

    PaxDbi P52482.
    PRIDEi P52482.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000022296 ; ENSMUSP00000022296 ; ENSMUSG00000021774 .
    GeneIDi 22194.
    KEGGi mmu:22194.
    UCSCi uc007shu.1. mouse.

    Organism-specific databases

    CTDi 7324.
    MGIi MGI:107411. Ube2e1.

    Phylogenomic databases

    eggNOGi COG5078.
    HOGENOMi HOG000233455.
    HOVERGENi HBG063308.
    InParanoidi P52482.
    KOi K06689.
    OMAi SIGTQYM.
    OrthoDBi EOG7PCJGX.
    PhylomeDBi P52482.
    TreeFami TF101117.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_198533. ISG15 antiviral mechanism.
    REACT_205250. Phosphorylation of the APC/C.
    REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
    REACT_207679. Separation of Sister Chromatids.
    REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
    REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
    REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
    REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
    REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
    REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.

    Miscellaneous databases

    NextBioi 302173.
    PROi P52482.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52482.
    Bgeei P52482.
    Genevestigatori P52482.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions."
      Matuschewski K., Hauser H.P., Treier M., Jentsch S.
      J. Biol. Chem. 271:2789-2794(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
      Tissue: Brain.
    2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Mammary tumor.

    Entry informationi

    Entry nameiUB2E1_MOUSE
    AccessioniPrimary (citable) accession number: P52482
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 121 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3