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P52482

- UB2E1_MOUSE

UniProt

P52482 - UB2E1_MOUSE

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Protein

Ubiquitin-conjugating enzyme E2 E1

Gene
Ube2e1, Ubce5, Ubcm3
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at transcript leveli

Functioni

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination By similarity.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei131 – 1311Glycyl thioester intermediate By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. ISG15 ligase activity Source: Ensembl
  3. ubiquitin-protein transferase activity Source: UniProtKB

GO - Biological processi

  1. histone H2B ubiquitination Source: Ensembl
  2. histone monoubiquitination Source: Ensembl
  3. ISG15-protein conjugation Source: Ensembl
  4. protein K48-linked ubiquitination Source: UniProtKB
  5. protein ubiquitination Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_198533. ISG15 antiviral mechanism.
REACT_205250. Phosphorylation of the APC/C.
REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
REACT_207679. Separation of Sister Chromatids.
REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 E1 (EC:6.3.2.19)
Alternative name(s):
UbcM3
Ubiquitin carrier protein E1
Ubiquitin-protein ligase E1
Gene namesi
Name:Ube2e1
Synonyms:Ubce5, Ubcm3
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 14

Organism-specific databases

MGIiMGI:107411. Ube2e1.

Subcellular locationi

Nucleus By similarity

GO - Cellular componenti

  1. nucleus Source: UniProtKB
  2. ubiquitin ligase complex Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 193192Ubiquitin-conjugating enzyme E2 E1PRO_0000082471Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylserine By similarity
Cross-linki136 – 136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity

Post-translational modificationi

ISGylation suppresses ubiquitin E2 enzyme activity By similarity.

Keywords - PTMi

Acetylation, Isopeptide bond, Ubl conjugation

Proteomic databases

PaxDbiP52482.
PRIDEiP52482.

PTM databases

PhosphoSiteiP52482.

Expressioni

Gene expression databases

ArrayExpressiP52482.
BgeeiP52482.
GenevestigatoriP52482.

Interactioni

Subunit structurei

Interacts with RNF14.

Protein-protein interaction databases

BioGridi204406. 2 interactions.
IntActiP52482. 1 interaction.
STRINGi10090.ENSMUSP00000022296.

Structurei

3D structure databases

ProteinModelPortaliP52482.
SMRiP52482. Positions 46-193.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi9 – 1810Poly-Ser

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG5078.
HOGENOMiHOG000233455.
HOVERGENiHBG063308.
InParanoidiP52482.
KOiK06689.
OMAiSIGTQYM.
OrthoDBiEOG7PCJGX.
PhylomeDBiP52482.
TreeFamiTF101117.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52482-1 [UniParc]FASTAAdd to Basket

« Hide

MSDDDSRAST SSSSSSSSNQ QTEKEGSTPK KKESKVSMSK NSKLLSTSAK    50
RIQKELADIT LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI 100
TFTPEYPFKP PKVTFRTRIY HCNINSQGVI CLDILKDNWS PALTISKVLL 150
SICSLLTDCN PADPLVGSIA TQYMTNRAEH DRMARQWTKR YAT 193
Length:193
Mass (Da):21,333
Last modified:October 1, 1996 - v1
Checksum:iB535B3095EF6C445
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92665 mRNA. Translation: CAA63353.1.
BC003781 mRNA. Translation: AAH03781.1.
CCDSiCCDS26837.1.
RefSeqiNP_033481.1. NM_009455.3.
UniGeneiMm.4429.

Genome annotation databases

EnsembliENSMUST00000022296; ENSMUSP00000022296; ENSMUSG00000021774.
GeneIDi22194.
KEGGimmu:22194.
UCSCiuc007shu.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X92665 mRNA. Translation: CAA63353.1 .
BC003781 mRNA. Translation: AAH03781.1 .
CCDSi CCDS26837.1.
RefSeqi NP_033481.1. NM_009455.3.
UniGenei Mm.4429.

3D structure databases

ProteinModelPortali P52482.
SMRi P52482. Positions 46-193.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 204406. 2 interactions.
IntActi P52482. 1 interaction.
STRINGi 10090.ENSMUSP00000022296.

PTM databases

PhosphoSitei P52482.

Proteomic databases

PaxDbi P52482.
PRIDEi P52482.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000022296 ; ENSMUSP00000022296 ; ENSMUSG00000021774 .
GeneIDi 22194.
KEGGi mmu:22194.
UCSCi uc007shu.1. mouse.

Organism-specific databases

CTDi 7324.
MGIi MGI:107411. Ube2e1.

Phylogenomic databases

eggNOGi COG5078.
HOGENOMi HOG000233455.
HOVERGENi HBG063308.
InParanoidi P52482.
KOi K06689.
OMAi SIGTQYM.
OrthoDBi EOG7PCJGX.
PhylomeDBi P52482.
TreeFami TF101117.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_198533. ISG15 antiviral mechanism.
REACT_205250. Phosphorylation of the APC/C.
REACT_206033. Senescence-Associated Secretory Phenotype (SASP).
REACT_207679. Separation of Sister Chromatids.
REACT_210462. APC/C:Cdc20 mediated degradation of Cyclin B.
REACT_215601. Conversion from APC/C:Cdc20 to APC/C:Cdh1 in late anaphase.
REACT_219897. APC/C:Cdc20 mediated degradation of Securin.
REACT_222875. APC/C:Cdc20 mediated degradation of mitotic proteins.
REACT_225686. Autodegradation of Cdh1 by Cdh1:APC/C.
REACT_226135. APC/C:Cdh1 mediated degradation of Cdc20 and other APC/C:Cdh1 targeted proteins in late mitosis/early G1.

Miscellaneous databases

NextBioi 302173.
PROi P52482.
SOURCEi Search...

Gene expression databases

ArrayExpressi P52482.
Bgeei P52482.
Genevestigatori P52482.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions."
    Matuschewski K., Hauser H.P., Treier M., Jentsch S.
    J. Biol. Chem. 271:2789-2794(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
    Tissue: Brain.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Mammary tumor.

Entry informationi

Entry nameiUB2E1_MOUSE
AccessioniPrimary (citable) accession number: P52482
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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