P52482 (UB2E1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 E1
EC=6.3.2.19

Alternative name(s):
UbcM3
Ubiquitin carrier protein E1
Ubiquitin-protein ligase E1

Gene names

Name:	Ube2e1
Synonyms:	Ubce5, Ubcm3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination By similarity.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RNF14.
Subcellular location	Nucleus By similarity.
Post-translational modification	ISGylation suppresses ubiquitin E2 enzyme activity By similarity.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Cellular component	Nucleus
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Isopeptide bond Phosphoprotein Ubl conjugation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	protein K48-linked ubiquitination Inferred from sequence or structural similarity. Source: UniProtKB
Cellular component	nucleus Inferred from sequence or structural similarity. Source: UniProtKB
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from sequence or structural similarity. Source: UniProtKB
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Ubiquitin-conjugating enzyme E2 E1		PRO_0000082471
Regions
	Compositional bias	9 – 18	10	Poly-Ser
Sites
	Active site	131	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	2	1	Phosphoserine By similarity
	Modified residue	9	1	Phosphoserine By similarity
	Modified residue	12	1	Phosphoserine By similarity
	Modified residue	77	1	Phosphotyrosine By similarity
	Cross-link	136		Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52482 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B535B3095EF6C445	FASTA	193	21,333
	10 20 30 40 50 60 MSDDDSRAST SSSSSSSSNQ QTEKEGSTPK KKESKVSMSK NSKLLSTSAK RIQKELADIT 70 80 90 100 110 120 LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY 130 140 150 160 170 180 HCNINSQGVI CLDILKDNWS PALTISKVLL SICSLLTDCN PADPLVGSIA TQYMTNRAEH 190 DRMARQWTKR YAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions." Matuschewski K., Hauser H.P., Treier M., Jentsch S. J. Biol. Chem. 271:2789-2794(1996) [PubMed: 8576256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X92665 mRNA. Translation: CAA63353.1. BC003781 mRNA. Translation: AAH03781.1.
IPI	IPI00127542.
RefSeq	NP_033481.1. NM_009455.3.
UniGene	Mm.4429.
3D structure databases
ProteinModelPortal	P52482.
SMR	P52482. Positions 46-193.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52482. 1 interaction.
STRING	P52482.
PTM databases
PhosphoSite	P52482.
Proteomic databases
PRIDE	P52482.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSMUST00000022296; ENSMUSP00000022296; ENSMUSG00000021774.
GeneID	22194.
KEGG	mmu:22194.
Organism-specific databases
CTD	7324.
MGI	MGI:107411. Ube2e1.
Phylogenomic databases
eggNOG	roNOG15669.
HOGENOM	HBG756483.
HOVERGEN	HBG063308.
InParanoid	P52482.
OMA	GKSRIQK.
OrthoDB	EOG48SGV9.
PhylomeDB	P52482.
Gene expression databases
ArrayExpress	P52482.
Bgee	P52482.
Genevestigator	P52482.
GermOnline	ENSMUSG00000021774. Mus musculus.
Family and domain databases
InterPro	IPR000608. UBQ-conjugat_E2. IPR023313. UBQ-conjugating_AS. IPR016135. UBQ-conjugating_enzyme/RWD. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
KO	K06689.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
SUPFAM	SSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	302173.
SOURCE	Search...

Entry information

Entry name

UB2E1_MOUSE

Accession

Primary (citable) accession number: P52482

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 16, 2011
This is version 102 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program