Reviewed, UniProtKB/Swiss-Prot P52482 (UB2E1_MOUSE)

Names and origin

Protein names

Recommended name:
    Ubiquitin-conjugating enzyme E2 E1
    EC=6.3.2.19
Alternative name(s):
    Ubiquitin-protein ligase E1
    Ubiquitin carrier protein E1
    UbcM3

Gene names

Name:	Ube2e1
Synonyms:	Ubce5, Ubcm3

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	193 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins. Mediates the selective degradation of short-lived and abnormal proteins.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with RNF14.
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
PTM	Phosphoprotein
Gene Ontology (GO)
Biological process	modification-dependent protein catabolic process Inferred from electronic annotation. Source: UniProtKB-KW post-translational protein modification Inferred from electronic annotation. Source: InterPro regulation of protein metabolic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin-protein ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 193	193	Ubiquitin-conjugating enzyme E2 E1		PRO_0000082471
Regions
	Compositional bias	9 – 18	10	Poly-Ser
Sites
	Active site	131	1	Glycyl thioester intermediate By similarity
Amino acid modifications
	Modified residue	77	1	Phosphotyrosine By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52482-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B535B3095EF6C445	FASTA	193	21,333
	10 20 30 40 50 60 MSDDDSRAST SSSSSSSSNQ QTEKEGSTPK KKESKVSMSK NSKLLSTSAK RIQKELADIT 70 80 90 100 110 120 LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY 130 140 150 160 170 180 HCNINSQGVI CLDILKDNWS PALTISKVLL SICSLLTDCN PADPLVGSIA TQYMTNRAEH 190 DRMARQWTKR YAT

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions." Matuschewski K., Hauser H.P., Treier M., Jentsch S. J. Biol. Chem. 271:2789-2794(1996) [PubMed: 8576256] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c. Tissue: Brain.
[2]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Tissue: Mammary tumor.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	X92665 mRNA. Translation: CAA63353.1. BC003781 mRNA. Translation: AAH03781.1.
IPI	IPI00127542.
RefSeq	NP_033481.1.
UniGene	Mm.4429
3D structure databases
HSSP	HSSP built from PDB template 1QCQ based on UniProtKB P15731.
SMR	P52482. Positions 46-193.
ModBase	Search...
Protein-protein interaction databases
STRING	P52482.
PTM databases
PhosphoSite	P52482.
Proteomic databases
PRIDE	P52482.
Genome annotation databases
Ensembl	ENSMUST00000022296; ENSMUSP00000022296; ENSMUSG00000021774; Mus musculus. [Genome view]
GeneID	22194.
KEGG	mmu:22194.
UCSC	uc007shu.1. mouse.
Organism-specific databases
CTD	22194.
MGI	MGI:107411. Ube2e1.
Phylogenomic databases
HOGENOM	P52482.
HOVERGEN	P52482.
OMA	KETNAPK.
Enzyme and pathway databases
BRENDA	6.3.2.19. 244.
Gene expression databases
ArrayExpress	P52482.
Bgee	P52482.
Genevestigator	P52482.
GermOnline	ENSMUSG00000021774. Mus musculus.
Family and domain databases
InterPro	IPR016135. UBQ-conjugat/RWD-like. IPR000608. UBQ-conjugat_E2. [Graphical view]
Gene3D	G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.
Pfam	PF00179. UQ_con. 1 hit. [Graphical view]
ProDom	PD000461. UBQ_conjugat. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00212. UBCc. 1 hit. [Graphical view]
PROSITE	PS00183. UBIQUITIN_CONJUGAT_1. 1 hit. PS50127. UBIQUITIN_CONJUGAT_2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	302173.
SOURCE	Search...

Entry information

Entry name

UB2E1_MOUSE

Accession

Primary (citable) accession number: P52482

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 3, 2009
This is version 86 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)