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P52482 (UB2E1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 117. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 E1

EC=6.3.2.19
Alternative name(s):
UbcM3
Ubiquitin carrier protein E1
Ubiquitin-protein ligase E1
Gene names
Name:Ube2e1
Synonyms:Ubce5, Ubcm3
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length193 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Accepts ubiquitin from the E1 complex and catalyzes its covalent attachment to other proteins. Catalyzes the covalent attachment of ISG15 to other proteins. Mediates the selective degradation of short-lived and abnormal proteins. In vitro also catalyzes 'Lys-48'-linked polyubiquitination By similarity.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with RNF14.

Subcellular location

Nucleus By similarity.

Post-translational modification

ISGylation suppresses ubiquitin E2 enzyme activity By similarity.

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Initiator methionine11Removed By similarity
Chain2 – 193192Ubiquitin-conjugating enzyme E2 E1
PRO_0000082471

Regions

Compositional bias9 – 1810Poly-Ser

Sites

Active site1311Glycyl thioester intermediate By similarity

Amino acid modifications

Modified residue21N-acetylserine By similarity
Cross-link136Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ISG15) By similarity

Sequences

Sequence LengthMass (Da)Tools
P52482 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B535B3095EF6C445

FASTA19321,333
        10         20         30         40         50         60 
MSDDDSRAST SSSSSSSSNQ QTEKEGSTPK KKESKVSMSK NSKLLSTSAK RIQKELADIT 

        70         80         90        100        110        120 
LDPPPNCSAG PKGDNIYEWR STILGPPGSV YEGGVFFLDI TFTPEYPFKP PKVTFRTRIY 

       130        140        150        160        170        180 
HCNINSQGVI CLDILKDNWS PALTISKVLL SICSLLTDCN PADPLVGSIA TQYMTNRAEH 

       190 
DRMARQWTKR YAT 

« Hide

References

« Hide 'large scale' references
[1]"Identification of a novel family of ubiquitin-conjugating enzymes with distinct amino-terminal extensions."
Matuschewski K., Hauser H.P., Treier M., Jentsch S.
J. Biol. Chem. 271:2789-2794(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: BALB/c.
Tissue: Brain.
[2]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Tissue: Mammary tumor.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X92665 mRNA. Translation: CAA63353.1.
BC003781 mRNA. Translation: AAH03781.1.
RefSeqNP_033481.1. NM_009455.3.
UniGeneMm.4429.

3D structure databases

ProteinModelPortalP52482.
SMRP52482. Positions 46-193.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

BioGrid204406. 2 interactions.
IntActP52482. 1 interaction.
STRING10090.ENSMUSP00000022296.

PTM databases

PhosphoSiteP52482.

Proteomic databases

PaxDbP52482.
PRIDEP52482.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblENSMUST00000022296; ENSMUSP00000022296; ENSMUSG00000021774.
GeneID22194.
KEGGmmu:22194.
UCSCuc007shu.1. mouse.

Organism-specific databases

CTD7324.
MGIMGI:107411. Ube2e1.

Phylogenomic databases

eggNOGCOG5078.
HOGENOMHOG000233455.
HOVERGENHBG063308.
InParanoidP52482.
KOK06689.
OMATANRAEH.
OrthoDBEOG7PCJGX.
PhylomeDBP52482.
TreeFamTF101117.

Enzyme and pathway databases

UniPathwayUPA00143.

Gene expression databases

ArrayExpressP52482.
BgeeP52482.
GenevestigatorP52482.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. SSF54495. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio302173.
PROP52482.
SOURCESearch...

Entry information

Entry nameUB2E1_MOUSE
AccessionPrimary (citable) accession number: P52482
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 117 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot