P52478 (UBC1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Ubiquitin-conjugating enzyme E2 1
EC=6.3.2.19

Alternative name(s):
Ubiquitin carrier protein 1
Ubiquitin-protein ligase 1

Gene names

Name:	ubc-1
ORF Names:	C35B1.1

Organism

Caenorhabditis elegans

Taxonomic identifier

6239 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length	192 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Catalyzes the covalent attachment of ubiquitin to other proteins.
Catalytic activity	ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.
Pathway	Protein modification; protein ubiquitination.
Subunit structure	Interacts with ubr-1 and rfp-1. Ref.3
Disruption phenotype	Worms are viable, fertile, and do not show any obvious anormality. Ref.3
Sequence similarities	Belongs to the ubiquitin-conjugating enzyme family.

Ontologies

Keywords
Biological process	Ubl conjugation pathway
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	3D-structure Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from direct assay Ref.1. Source: WormBase growth Inferred from mutant phenotype. Source: WormBase nematode larval development Inferred from mutant phenotype. Source: WormBase reproduction Inferred from mutant phenotype. Source: WormBase
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW ubiquitin protein ligase binding Inferred from physical interaction Ref.3. Source: WormBase ubiquitin-protein ligase activity Inferred from direct assay Ref.1. Source: WormBase
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
rfp-1	P34537	7	EBI-316677,EBI-316712

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 192

192

Ubiquitin-conjugating enzyme E2 1

PRO_0000082514

Sites

Active site

88

1

Glycyl thioester intermediate By similarity

Secondary structure

1

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192

Helix Strand Turn

Details...

Helix

4 – 18

15

Beta strand

24 – 28

5

Beta strand

35 – 41

7

Beta strand

44 – 46

3

Turn

47 – 50

4

Beta strand

52 – 58

7

Turn

61 – 65

5

Beta strand

69 – 74

6

Beta strand

85 – 87

3

Helix

90 – 92

3

Turn

93 – 95

3

Helix

102 – 114

13

Helix

124 – 131

8

Helix

134 – 147

14

Sequences

	Sequence		Length	Mass (Da)
	P52478 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 7CF26B8FB96EF33D	FASTA	192	21,513
	10 20 30 40 50 60 MTTPSRRRLM RDFKKLQEDP PAGVSGAPTE DNILTWEAII FGPQETPFED GTFKLSLEFT 70 80 90 100 110 120 EEYPNKPPTV KFISKMFHPN VYADGSICLD ILQNRWSPTY DVAAILTSIQ SLLDEPNPNS 130 140 150 160 170 180 PANSLAAQLY QENRREYEKR VQQIVEQSWL NFGENEGDAV LKDDVEIEEI AAPGANDADD 190 DRMDEGASGS NA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Caenorhabditis elegans UBC-1, a ubiquitin-conjugating enzyme homologous to yeast RAD6/UBC2, contains a novel carboxy-terminal extension that is conserved in nematodes." Leggett D.S., Jones D., Candido E.P.M. DNA Cell Biol. 14:883-891(1995) [PubMed: 7546294] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: Bristol N2.
[2]	"Genome sequence of the nematode C. elegans: a platform for investigating biology." The C. elegans sequencing consortium Science 282:2012-2018(1998) [PubMed: 9851916] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Bristol N2.
[3]	"Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1." Crowe E., Candido E.P.M. Dev. Biol. 265:446-459(2004) [PubMed: 14732404] [Abstract] Cited for: INTERACTION WITH UBR-1 AND RFP-1, DISRUPTION PHENOTYPE.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

U08139 Genomic DNA. Translation: AAA83388.1.
FO080784 Genomic DNA. Translation: CCD66736.1.

PIR

T32959.

RefSeq

NP_500480.1. NM_068079.4.

UniGene

Cel.18169.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
1Q34	X-ray	2.90	A/B/C	1-163	[»]
1Z3D	X-ray	2.50	A	1-154	[»]

ProteinModelPortal

P52478.

SMR

P52478. Positions 2-152.

ModBase

Search...

Protein-protein interaction databases

IntAct

P52478. 8 interactions.

MINT

MINT-226915.

STRING

P52478.

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblMetazoa

C35B1.1.1; C35B1.1.1; C35B1.1.
C35B1.1.2; C35B1.1.2; C35B1.1.

GeneID

177170.

KEGG

cel:C35B1.1.

UCSC

C35B1.1. c. elegans.

Organism-specific databases

CTD

177170.

WormBase

C35B1.1; CE27822; WBGene00006701; ubc-1.

Phylogenomic databases

eggNOG

meNOG05975.

GeneTree

EMGT00050000007017.

HOGENOM

HBG756483.

InParanoid

P52478.

OMA

NGELCLD.

PhylomeDB

P52478.

Gene expression databases

ArrayExpress

P52478.

Family and domain databases

InterPro

IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]

Gene3D

G3DSA:3.10.110.10. UBQ-conjugat_E2. 1 hit.

KO

K10573.

Pfam

PF00179. UQ_con. 1 hit.
[Graphical view]

SUPFAM

SSF54495. UBQ-conjugat/RWD-like. 1 hit.

PROSITE

PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Other

NextBio

895652.

Entry information

Entry name

UBC1_CAEEL

Accession

Primary (citable) accession number: P52478
Secondary accession number(s): O45062

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	December 14, 2011
This is version 97 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Caenorhabditis annotation project