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Protein

Ubiquitin-conjugating enzyme E2 1

Gene

ubc-1

Organism
Caenorhabditis elegans
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activityi

S-ubiquitinyl-[E1 ubiquitin-activating enzyme]-L-cysteine + [E2 ubiquitin-conjugating enzyme]-L-cysteine = [E1 ubiquitin-activating enzyme]-L-cysteine + S-ubiquitinyl-[E2 ubiquitin-conjugating enzyme]-L-cysteine.PROSITE-ProRule annotationCurated

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.PROSITE-ProRule annotation
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei88Glycyl thioester intermediatePROSITE-ProRule annotation1

GO - Molecular functioni

  • ATP binding Source: UniProtKB-KW
  • ubiquitin conjugating enzyme activity Source: WormBase
  • ubiquitin protein ligase activity Source: GO_Central
  • ubiquitin protein ligase binding Source: WormBase

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiR-CEL-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-CEL-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 1 (EC:2.3.2.23)
Alternative name(s):
E2 ubiquitin-conjugating enzyme 1
Ubiquitin carrier protein 1
Ubiquitin-protein ligase 1
Gene namesi
Name:ubc-1
ORF Names:C35B1.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
Proteomesi
  • UP000001940 Componenti: Chromosome IV

Organism-specific databases

WormBaseiC35B1.1; CE27822; WBGene00006701; ubc-1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • HULC complex Source: GO_Central
  • nuclear chromatin Source: GO_Central
  • ubiquitin conjugating enzyme complex Source: WormBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Worms are viable, fertile, and do not show any obvious anomaly.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000825141 – 192Ubiquitin-conjugating enzyme E2 1Add BLAST192

Proteomic databases

EPDiP52478.
PaxDbiP52478.
PeptideAtlasiP52478.
PRIDEiP52478.

Expressioni

Gene expression databases

BgeeiWBGene00006701.

Interactioni

Subunit structurei

Interacts with ubr-1 and rfp-1 (PubMed:14732404). Interacts with ubc-13 (PubMed:15530417).2 Publications

Binary interactionsi

WithEntry#Exp.IntActNotes
rfp-1P345377EBI-316677,EBI-316712

GO - Molecular functioni

  • ubiquitin protein ligase binding Source: WormBase

Protein-protein interaction databases

BioGridi42306. 12 interactors.
IntActiP52478. 8 interactors.
MINTiMINT-226915.
STRINGi6239.C35B1.1.1.

Structurei

Secondary structure

1192
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi4 – 18Combined sources15
Beta strandi24 – 29Combined sources6
Beta strandi32 – 41Combined sources10
Beta strandi44 – 46Combined sources3
Turni47 – 50Combined sources4
Beta strandi52 – 58Combined sources7
Turni61 – 65Combined sources5
Beta strandi69 – 74Combined sources6
Beta strandi85 – 87Combined sources3
Helixi90 – 92Combined sources3
Turni93 – 95Combined sources3
Helixi102 – 111Combined sources10
Helixi124 – 132Combined sources9
Helixi134 – 148Combined sources15
Turni149 – 151Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q34X-ray2.90A/B/C1-163[»]
1Z3DX-ray2.50A1-154[»]
ProteinModelPortaliP52478.
SMRiP52478.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52478.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiKOG0419. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
InParanoidiP52478.
KOiK10573.
OMAiNEYPNRP.
OrthoDBiEOG091G0UM8.
PhylomeDBiP52478.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52478-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTTPSRRRLM RDFKKLQEDP PAGVSGAPTE DNILTWEAII FGPQETPFED
60 70 80 90 100
GTFKLSLEFT EEYPNKPPTV KFISKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVAAILTSIQ SLLDEPNPNS PANSLAAQLY QENRREYEKR VQQIVEQSWL
160 170 180 190
NFGENEGDAV LKDDVEIEEI AAPGANDADD DRMDEGASGS NA
Length:192
Mass (Da):21,513
Last modified:October 1, 1996 - v1
Checksum:i7CF26B8FB96EF33D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08139 Genomic DNA. Translation: AAA83388.1.
FO080784 Genomic DNA. Translation: CCD66736.1.
PIRiT32959.
RefSeqiNP_500480.1. NM_068079.5.
UniGeneiCel.18169.

Genome annotation databases

EnsemblMetazoaiC35B1.1; C35B1.1; WBGene00006701.
GeneIDi177170.
KEGGicel:CELE_C35B1.1.
UCSCiC35B1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U08139 Genomic DNA. Translation: AAA83388.1.
FO080784 Genomic DNA. Translation: CCD66736.1.
PIRiT32959.
RefSeqiNP_500480.1. NM_068079.5.
UniGeneiCel.18169.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1Q34X-ray2.90A/B/C1-163[»]
1Z3DX-ray2.50A1-154[»]
ProteinModelPortaliP52478.
SMRiP52478.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi42306. 12 interactors.
IntActiP52478. 8 interactors.
MINTiMINT-226915.
STRINGi6239.C35B1.1.1.

Proteomic databases

EPDiP52478.
PaxDbiP52478.
PeptideAtlasiP52478.
PRIDEiP52478.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiC35B1.1; C35B1.1; WBGene00006701.
GeneIDi177170.
KEGGicel:CELE_C35B1.1.
UCSCiC35B1.1. c. elegans.

Organism-specific databases

CTDi177170.
WormBaseiC35B1.1; CE27822; WBGene00006701; ubc-1.

Phylogenomic databases

eggNOGiKOG0419. Eukaryota.
COG5078. LUCA.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
InParanoidiP52478.
KOiK10573.
OMAiNEYPNRP.
OrthoDBiEOG091G0UM8.
PhylomeDBiP52478.

Enzyme and pathway databases

UniPathwayiUPA00143.
ReactomeiR-CEL-8866652. Synthesis of active ubiquitin: roles of E1 and E2 enzymes.
R-CEL-983168. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTraceiP52478.
PROiP52478.

Gene expression databases

BgeeiWBGene00006701.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiUBC1_CAEEL
AccessioniPrimary (citable) accession number: P52478
Secondary accession number(s): O45062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 139 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.