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P52478

- UBC1_CAEEL

UniProt

P52478 - UBC1_CAEEL

Protein

Ubiquitin-conjugating enzyme E2 1

Gene

ubc-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 119 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalyzes the covalent attachment of ubiquitin to other proteins.

    Catalytic activityi

    ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

    GO - Molecular functioni

    1. acid-amino acid ligase activity Source: InterPro
    2. ATP binding Source: UniProtKB-KW
    3. damaged DNA binding Source: WormBase
    4. protein binding Source: IntAct
    5. ubiquitin conjugating enzyme activity Source: WormBase
    6. ubiquitin protein ligase binding Source: WormBase

    GO - Biological processi

    1. DNA repair Source: WormBase
    2. protein ubiquitination Source: WormBase

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Ubl conjugation pathway

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    ReactomeiREACT_183296. Antigen processing: Ubiquitination & Proteasome degradation.
    UniPathwayiUPA00143.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Ubiquitin-conjugating enzyme E2 1 (EC:6.3.2.19)
    Alternative name(s):
    Ubiquitin carrier protein 1
    Ubiquitin-protein ligase 1
    Gene namesi
    Name:ubc-1
    ORF Names:C35B1.1
    OrganismiCaenorhabditis elegans
    Taxonomic identifieri6239 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
    ProteomesiUP000001940: Chromosome IV

    Organism-specific databases

    WormBaseiC35B1.1; CE27822; WBGene00006701; ubc-1.

    Subcellular locationi

    GO - Cellular componenti

    1. ubiquitin conjugating enzyme complex Source: WormBase

    Pathology & Biotechi

    Disruption phenotypei

    Worms are viable, fertile, and do not show any obvious anomaly.1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 192192Ubiquitin-conjugating enzyme E2 1PRO_0000082514Add
    BLAST

    Proteomic databases

    PaxDbiP52478.

    Interactioni

    Subunit structurei

    Interacts with ubr-1 and rfp-1.1 Publication

    Binary interactionsi

    WithEntry#Exp.IntActNotes
    rfp-1P345377EBI-316677,EBI-316712

    Protein-protein interaction databases

    BioGridi42306. 12 interactions.
    IntActiP52478. 8 interactions.
    MINTiMINT-226915.

    Structurei

    Secondary structure

    1
    192
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Helixi4 – 1815
    Beta strandi24 – 296
    Beta strandi32 – 4110
    Beta strandi44 – 463
    Turni47 – 504
    Beta strandi52 – 587
    Turni61 – 655
    Beta strandi69 – 746
    Beta strandi85 – 873
    Helixi90 – 923
    Turni93 – 953
    Helixi102 – 11110
    Helixi124 – 1329
    Helixi134 – 14815
    Turni149 – 1513

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1Q34X-ray2.90A/B/C1-163[»]
    1Z3DX-ray2.50A1-154[»]
    ProteinModelPortaliP52478.
    SMRiP52478. Positions 2-152.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52478.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

    Phylogenomic databases

    eggNOGiCOG5078.
    GeneTreeiENSGT00730000110565.
    HOGENOMiHOG000233454.
    InParanoidiP52478.
    KOiK10573.
    OMAiIVEQSWV.
    PhylomeDBiP52478.

    Family and domain databases

    Gene3Di3.10.110.10. 1 hit.
    InterProiIPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view]
    PfamiPF00179. UQ_con. 1 hit.
    [Graphical view]
    SUPFAMiSSF54495. SSF54495. 1 hit.
    PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52478-1 [UniParc]FASTAAdd to Basket

    « Hide

    MTTPSRRRLM RDFKKLQEDP PAGVSGAPTE DNILTWEAII FGPQETPFED    50
    GTFKLSLEFT EEYPNKPPTV KFISKMFHPN VYADGSICLD ILQNRWSPTY 100
    DVAAILTSIQ SLLDEPNPNS PANSLAAQLY QENRREYEKR VQQIVEQSWL 150
    NFGENEGDAV LKDDVEIEEI AAPGANDADD DRMDEGASGS NA 192
    Length:192
    Mass (Da):21,513
    Last modified:October 1, 1996 - v1
    Checksum:i7CF26B8FB96EF33D
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08139 Genomic DNA. Translation: AAA83388.1.
    FO080784 Genomic DNA. Translation: CCD66736.1.
    PIRiT32959.
    RefSeqiNP_500480.1. NM_068079.5.
    UniGeneiCel.18169.

    Genome annotation databases

    EnsemblMetazoaiC35B1.1; C35B1.1; WBGene00006701.
    GeneIDi177170.
    KEGGicel:CELE_C35B1.1.
    UCSCiC35B1.1. c. elegans.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U08139 Genomic DNA. Translation: AAA83388.1 .
    FO080784 Genomic DNA. Translation: CCD66736.1 .
    PIRi T32959.
    RefSeqi NP_500480.1. NM_068079.5.
    UniGenei Cel.18169.

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1Q34 X-ray 2.90 A/B/C 1-163 [» ]
    1Z3D X-ray 2.50 A 1-154 [» ]
    ProteinModelPortali P52478.
    SMRi P52478. Positions 2-152.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 42306. 12 interactions.
    IntActi P52478. 8 interactions.
    MINTi MINT-226915.

    Proteomic databases

    PaxDbi P52478.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblMetazoai C35B1.1 ; C35B1.1 ; WBGene00006701 .
    GeneIDi 177170.
    KEGGi cel:CELE_C35B1.1.
    UCSCi C35B1.1. c. elegans.

    Organism-specific databases

    CTDi 177170.
    WormBasei C35B1.1 ; CE27822 ; WBGene00006701 ; ubc-1.

    Phylogenomic databases

    eggNOGi COG5078.
    GeneTreei ENSGT00730000110565.
    HOGENOMi HOG000233454.
    InParanoidi P52478.
    KOi K10573.
    OMAi IVEQSWV.
    PhylomeDBi P52478.

    Enzyme and pathway databases

    UniPathwayi UPA00143 .
    Reactomei REACT_183296. Antigen processing: Ubiquitination & Proteasome degradation.

    Miscellaneous databases

    EvolutionaryTracei P52478.
    NextBioi 895652.
    PROi P52478.

    Family and domain databases

    Gene3Di 3.10.110.10. 1 hit.
    InterProi IPR000608. UBQ-conjugat_E2.
    IPR023313. UBQ-conjugating_AS.
    IPR016135. UBQ-conjugating_enzyme/RWD.
    [Graphical view ]
    Pfami PF00179. UQ_con. 1 hit.
    [Graphical view ]
    SUPFAMi SSF54495. SSF54495. 1 hit.
    PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
    PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Caenorhabditis elegans UBC-1, a ubiquitin-conjugating enzyme homologous to yeast RAD6/UBC2, contains a novel carboxy-terminal extension that is conserved in nematodes."
      Leggett D.S., Jones D., Candido E.P.M.
      DNA Cell Biol. 14:883-891(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: Bristol N2.
    2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
      The C. elegans sequencing consortium
      Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Bristol N2.
    3. "Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1."
      Crowe E., Candido E.P.M.
      Dev. Biol. 265:446-459(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH UBR-1 AND RFP-1, DISRUPTION PHENOTYPE.

    Entry informationi

    Entry nameiUBC1_CAEEL
    AccessioniPrimary (citable) accession number: P52478
    Secondary accession number(s): O45062
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 119 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programCaenorhabditis annotation project

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Complete proteome, Reference proteome

    Documents

    1. Caenorhabditis elegans
      Caenorhabditis elegans: entries, gene names and cross-references to WormBase
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    4. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3