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P52478 (UBC1_CAEEL) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 109. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (4) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Ubiquitin-conjugating enzyme E2 1
EC=6.3.2.19
Alternative name(s):
Ubiquitin carrier protein 1
Ubiquitin-protein ligase 1
Gene names
Name:ubc-1
ORF Names:C35B1.1
OrganismCaenorhabditis elegans [Reference proteome]
Taxonomic identifier6239 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Ecdysozoa › Nematoda › Chromadorea › Rhabditida › Rhabditoidea › Rhabditidae › Peloderinae › Caenorhabditis

Protein attributes

Sequence length192 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activity

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.

Pathway

Protein modification; protein ubiquitination.

Subunit structure

Interacts with ubr-1 and rfp-1. Ref.3

Disruption phenotype

Worms are viable, fertile, and do not show any obvious anomaly. Ref.3

Sequence similarities

Belongs to the ubiquitin-conjugating enzyme family.

Binary interactions

With

Entry

#Exp.

IntAct

Notes

rfp-1P345377EBI-316677,EBI-316712

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 192192Ubiquitin-conjugating enzyme E2 1
PRO_0000082514

Sites

Active site881Glycyl thioester intermediate By similarity

Secondary structure

1 ............................ 192
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
P52478 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 7CF26B8FB96EF33D

FASTA19221,513
        10         20         30         40         50         60 
MTTPSRRRLM RDFKKLQEDP PAGVSGAPTE DNILTWEAII FGPQETPFED GTFKLSLEFT 

        70         80         90        100        110        120 
EEYPNKPPTV KFISKMFHPN VYADGSICLD ILQNRWSPTY DVAAILTSIQ SLLDEPNPNS 

       130        140        150        160        170        180 
PANSLAAQLY QENRREYEKR VQQIVEQSWL NFGENEGDAV LKDDVEIEEI AAPGANDADD 

       190 
DRMDEGASGS NA

« Hide

References

« Hide 'large scale' references
[1]"Caenorhabditis elegans UBC-1, a ubiquitin-conjugating enzyme homologous to yeast RAD6/UBC2, contains a novel carboxy-terminal extension that is conserved in nematodes."
Leggett D.S., Jones D., Candido E.P.M.
DNA Cell Biol. 14:883-891(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: Bristol N2.
[2]"Genome sequence of the nematode C. elegans: a platform for investigating biology."
The C. elegans sequencing consortium
Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Bristol N2.
[3]"Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1."
Crowe E., Candido E.P.M.
Dev. Biol. 265:446-459(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH UBR-1 AND RFP-1, DISRUPTION PHENOTYPE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		U08139 Genomic DNA. Translation: AAA83388.1.
FO080784 Genomic DNA. Translation: CCD66736.1.
PIRT32959.
RefSeqNP_500480.1. NM_068079.5.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q34X-ray2.90A/B/C1-163[»]
1Z3DX-ray2.50A1-154[»]
ProteinModelPortalP52478.
SMRP52478. Positions 2-152.
ModBaseSearch...

Protein-protein interaction databases

IntActP52478. 8 interactions.
MINTMINT-226915.

Proteomic databases

PaxDbP52478.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaC35B1.1.1; C35B1.1.1; C35B1.1.
C35B1.1.2; C35B1.1.2; C35B1.1.
GeneID177170.
KEGGcel:CELE_C35B1.1.
UCSCC35B1.1. c. elegans.

Organism-specific databases

CTD177170.
WormBaseC35B1.1; CE27822; WBGene00006701; ubc-1.

Phylogenomic databases

eggNOGCOG5078.
GeneTreeENSGT00680000099547.
HOGENOMHOG000233454.
InParanoidP52478.
KOK10573.
OMAYANGELC.

Enzyme and pathway databases

UniPathwayUPA00143.

Family and domain databases

Gene3D3.10.110.10. 1 hit.
InterProIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMSSF54495. UBQ-conjugat/RWD-like. 1 hit.
PROSITEPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

EvolutionaryTraceP52478.
NextBio895652.

Entry information

Entry nameUBC1_CAEEL
AccessionPrimary (citable) accession number: P52478
Secondary accession number(s): O45062
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 1, 2013
This is version 109 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Relevant documents

Caenorhabditis elegans

Caenorhabditis elegans: entries, gene names and cross-references to WormBase

PATHWAY comments

Index of metabolic and biosynthesis pathways

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

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