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P52478

- UBC1_CAEEL

UniProt

P52478 - UBC1_CAEEL

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Protein

Ubiquitin-conjugating enzyme E2 1

Gene

ubc-1

Organism
Caenorhabditis elegans
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Catalyzes the covalent attachment of ubiquitin to other proteins.

Catalytic activityi

ATP + ubiquitin + protein lysine = AMP + diphosphate + protein N-ubiquityllysine.PROSITE-ProRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei88 – 881Glycyl thioester intermediatePROSITE-ProRule annotation

GO - Molecular functioni

  1. acid-amino acid ligase activity Source: InterPro
  2. ATP binding Source: UniProtKB-KW
  3. damaged DNA binding Source: WormBase
  4. ubiquitin conjugating enzyme activity Source: WormBase
  5. ubiquitin protein ligase binding Source: WormBase

GO - Biological processi

  1. DNA repair Source: WormBase
  2. protein ubiquitination Source: WormBase
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Ubl conjugation pathway

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

ReactomeiREACT_183296. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Ubiquitin-conjugating enzyme E2 1 (EC:6.3.2.19)
Alternative name(s):
Ubiquitin carrier protein 1
Ubiquitin-protein ligase 1
Gene namesi
Name:ubc-1
ORF Names:C35B1.1
OrganismiCaenorhabditis elegans
Taxonomic identifieri6239 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaNematodaChromadoreaRhabditidaRhabditoideaRhabditidaePeloderinaeCaenorhabditis
ProteomesiUP000001940: Chromosome IV

Organism-specific databases

WormBaseiC35B1.1; CE27822; WBGene00006701; ubc-1.

Subcellular locationi

GO - Cellular componenti

  1. ubiquitin conjugating enzyme complex Source: WormBase
Complete GO annotation...

Pathology & Biotechi

Disruption phenotypei

Worms are viable, fertile, and do not show any obvious anomaly.1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 192192Ubiquitin-conjugating enzyme E2 1PRO_0000082514Add
BLAST

Proteomic databases

PaxDbiP52478.

Interactioni

Subunit structurei

Interacts with ubr-1 and rfp-1.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
rfp-1P345377EBI-316677,EBI-316712

Protein-protein interaction databases

BioGridi42306. 12 interactions.
IntActiP52478. 8 interactions.
MINTiMINT-226915.

Structurei

Secondary structure

1
192
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi4 – 1815Combined sources
Beta strandi24 – 296Combined sources
Beta strandi32 – 4110Combined sources
Beta strandi44 – 463Combined sources
Turni47 – 504Combined sources
Beta strandi52 – 587Combined sources
Turni61 – 655Combined sources
Beta strandi69 – 746Combined sources
Beta strandi85 – 873Combined sources
Helixi90 – 923Combined sources
Turni93 – 953Combined sources
Helixi102 – 11110Combined sources
Helixi124 – 1329Combined sources
Helixi134 – 14815Combined sources
Turni149 – 1513Combined sources

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1Q34X-ray2.90A/B/C1-163[»]
1Z3DX-ray2.50A1-154[»]
ProteinModelPortaliP52478.
SMRiP52478. Positions 2-152.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52478.

Family & Domainsi

Sequence similaritiesi

Belongs to the ubiquitin-conjugating enzyme family.PROSITE-ProRule annotation

Phylogenomic databases

eggNOGiCOG5078.
GeneTreeiENSGT00730000110565.
HOGENOMiHOG000233454.
InParanoidiP52478.
KOiK10573.
OMAiIVEQSWV.
PhylomeDBiP52478.

Family and domain databases

Gene3Di3.10.110.10. 1 hit.
InterProiIPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view]
PfamiPF00179. UQ_con. 1 hit.
[Graphical view]
SUPFAMiSSF54495. SSF54495. 1 hit.
PROSITEiPS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52478 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MTTPSRRRLM RDFKKLQEDP PAGVSGAPTE DNILTWEAII FGPQETPFED
60 70 80 90 100
GTFKLSLEFT EEYPNKPPTV KFISKMFHPN VYADGSICLD ILQNRWSPTY
110 120 130 140 150
DVAAILTSIQ SLLDEPNPNS PANSLAAQLY QENRREYEKR VQQIVEQSWL
160 170 180 190
NFGENEGDAV LKDDVEIEEI AAPGANDADD DRMDEGASGS NA
Length:192
Mass (Da):21,513
Last modified:October 1, 1996 - v1
Checksum:i7CF26B8FB96EF33D
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08139 Genomic DNA. Translation: AAA83388.1.
FO080784 Genomic DNA. Translation: CCD66736.1.
PIRiT32959.
RefSeqiNP_500480.1. NM_068079.5.
UniGeneiCel.18169.

Genome annotation databases

EnsemblMetazoaiC35B1.1; C35B1.1; WBGene00006701.
GeneIDi177170.
KEGGicel:CELE_C35B1.1.
UCSCiC35B1.1. c. elegans.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U08139 Genomic DNA. Translation: AAA83388.1 .
FO080784 Genomic DNA. Translation: CCD66736.1 .
PIRi T32959.
RefSeqi NP_500480.1. NM_068079.5.
UniGenei Cel.18169.

3D structure databases

Select the link destinations:
PDBe
RCSB PDB
PDBj
Links Updated
Entry Method Resolution (Å) Chain Positions PDBsum
1Q34 X-ray 2.90 A/B/C 1-163 [» ]
1Z3D X-ray 2.50 A 1-154 [» ]
ProteinModelPortali P52478.
SMRi P52478. Positions 2-152.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

BioGridi 42306. 12 interactions.
IntActi P52478. 8 interactions.
MINTi MINT-226915.

Proteomic databases

PaxDbi P52478.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblMetazoai C35B1.1 ; C35B1.1 ; WBGene00006701 .
GeneIDi 177170.
KEGGi cel:CELE_C35B1.1.
UCSCi C35B1.1. c. elegans.

Organism-specific databases

CTDi 177170.
WormBasei C35B1.1 ; CE27822 ; WBGene00006701 ; ubc-1.

Phylogenomic databases

eggNOGi COG5078.
GeneTreei ENSGT00730000110565.
HOGENOMi HOG000233454.
InParanoidi P52478.
KOi K10573.
OMAi IVEQSWV.
PhylomeDBi P52478.

Enzyme and pathway databases

UniPathwayi UPA00143 .
Reactomei REACT_183296. Antigen processing: Ubiquitination & Proteasome degradation.

Miscellaneous databases

EvolutionaryTracei P52478.
NextBioi 895652.
PROi P52478.

Family and domain databases

Gene3Di 3.10.110.10. 1 hit.
InterProi IPR000608. UBQ-conjugat_E2.
IPR023313. UBQ-conjugating_AS.
IPR016135. UBQ-conjugating_enzyme/RWD.
[Graphical view ]
Pfami PF00179. UQ_con. 1 hit.
[Graphical view ]
SUPFAMi SSF54495. SSF54495. 1 hit.
PROSITEi PS00183. UBIQUITIN_CONJUGAT_1. 1 hit.
PS50127. UBIQUITIN_CONJUGAT_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Caenorhabditis elegans UBC-1, a ubiquitin-conjugating enzyme homologous to yeast RAD6/UBC2, contains a novel carboxy-terminal extension that is conserved in nematodes."
    Leggett D.S., Jones D., Candido E.P.M.
    DNA Cell Biol. 14:883-891(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: Bristol N2.
  2. "Genome sequence of the nematode C. elegans: a platform for investigating biology."
    The C. elegans sequencing consortium
    Science 282:2012-2018(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Bristol N2.
  3. "Characterization of C. elegans RING finger protein 1, a binding partner of ubiquitin-conjugating enzyme 1."
    Crowe E., Candido E.P.M.
    Dev. Biol. 265:446-459(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH UBR-1 AND RFP-1, DISRUPTION PHENOTYPE.

Entry informationi

Entry nameiUBC1_CAEEL
AccessioniPrimary (citable) accession number: P52478
Secondary accession number(s): O45062
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 120 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programCaenorhabditis annotation project

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Reference proteome

Documents

  1. Caenorhabditis elegans
    Caenorhabditis elegans: entries, gene names and cross-references to WormBase
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3