Reviewed, UniProtKB/Swiss-Prot P52476 (AL1B1_BOVIN)

Names and origin

Protein names

Recommended name:
    Aldehyde dehydrogenase X, mitochondrial
    EC=1.2.1.3
Alternative name(s):
    Aldehyde dehydrogenase family 1 member B1
    ALDH class 2
    ALDHX

Gene names

Name:	ALDH1B1
Synonyms:	ALDH1B2, ALDH5, ALDHX

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	511 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. In the cornea, this enzyme may help in the absorption of the damaging UV-B, as well as in the detoxification of the UV-induced peroxidic aldehydes.
Catalytic activity	An aldehyde + NAD+ + H2O = an acid + NADH.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 11	›11	Mitochondrion Potential
	Chain	12 – 511	500	Aldehyde dehydrogenase X, mitochondrial		PRO_0000056477
Regions
	Nucleotide binding	256 – 261	6	NAD By similarity
Sites
	Active site	279	1	Proton acceptor By similarity
	Active site	313	1	Nucleophile By similarity
	Site	180	1	Transition state stabilizer By similarity
Experimental info
	Sequence conflict	308	1	N → S in AAB26659. Ref.2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P52476-1 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: D032C6E84D9799B3	FASTA	511	56,770
	10 20 30 40 50 60 PRLFALHHSA TQYFSAAALP SPIPNPDIPD NQLFISNKWH DAVSKKTFPT VSPATGEVIG 70 80 90 100 110 120 HVAEGDWADV DLAAKAARAA FRLGSPWRWM DALKRGWLLN HLADLVERDC VYLASLESLD 130 140 150 160 170 180 NGKPFQESYV LDLDEVIKVY RYFAGWADKW HGKTIPMDGE HFCFTRHEPV GVCCQIIPWN 190 200 210 220 230 240 FPLVMQSWKL ALALAMGNTV VTKVAEQTPF SALYLASLIK EVGLPPGLVN IVTGYGPTAG 250 260 270 280 290 300 AAIAHHMDIG KVAFTGSTKV GHLIQKAAGN SSLKRVTLEL GGKSLSIVLA DADMDHAVEQ 310 320 330 340 350 360 RQEALFFNMG QCCCPGSWTF IEESIYDEFL ERTVEKAKQR RVGNPFDLDT QQGPQVDRER 370 380 390 400 410 420 FERILGYIQL GQKEGAKLLC GGEHFRQQCF FIKPTVFGGV QDDMRIAREE IFGPVQPLFK 430 440 450 460 470 480 FKKIEEVIER ADNTRYGLAA AVFTQDLDKA MYFTQALQTG TVWVNTYNVV TCHTPLGGFK 490 500 510 EPGNGRELGE DGLKAYTEVK TVTIKVPQKN S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed: 19390049] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hereford.
[2]	"Bovine corneal aldehyde dehydrogenases: evidence for multiple gene products (ALDH3 and ALDHX)." Algar E.M., Cheung B., Hayes J., Holmes R.S., Beacham I.R. Adv. Exp. Med. Biol. 328:153-157(1993) [PubMed: 8493893] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 284-409. Tissue: Cornea.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AAFC03050684 Genomic DNA. No translation available. S61045 mRNA. Translation: AAB26659.1.
IPI	IPI00908070.
PIR	I46935.
UniGene	Bt.13145
3D structure databases
HSSP	HSSP built from PDB template 1O02 based on UniProtKB P05091.
ModBase	Search...
Genome annotation databases
Ensembl	ENSBTAG00000020385. Bos taurus. [Contig view]
Phylogenomic databases
HOVERGEN	P52476.
OMA	P52476. EALFFNM.
Enzyme and pathway databases
BRENDA	1.2.1.3. 251.
Family and domain databases
InterPro	IPR016160. Ald_DH_CS. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH. [Graphical view]
Gene3D	G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11699. Aldehyde_dehyd. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL1B1_BOVIN

Accession

Primary (citable) accession number: P52476

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 9, 2007
Last modified:	June 16, 2009
This is version 65 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)