P52476 (AL1B1_BOVIN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Aldehyde dehydrogenase X, mitochondrial
EC=1.2.1.3

Alternative name(s):
ALDH class 2
ALDHX
Aldehyde dehydrogenase family 1 member B1

Gene names

Name:	ALDH1B1
Synonyms:	ALDH1B2, ALDH5, ALDHX

Organism

Bos taurus (Bovine) [Reference proteome]

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Dipnotetrapodomorpha › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	511 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	ALDHs play a major role in the detoxification of alcohol-derived acetaldehyde. They are involved in the metabolism of corticosteroids, biogenic amines, neurotransmitters, and lipid peroxidation. In the cornea, this enzyme may help in the absorption of the damaging UV-B, as well as in the detoxification of the UV-induced peroxidic aldehydes.
Catalytic activity	An aldehyde + NAD+ + H2O = a carboxylate + NADH.
Pathway	Alcohol metabolism; ethanol degradation; acetate from ethanol: step 2/2.
Subunit structure	Homotetramer By similarity.
Subcellular location	Mitochondrion matrix By similarity.
Sequence similarities	Belongs to the aldehyde dehydrogenase family.

Ontologies

Keywords
Cellular component	Mitochondrion
Domain	Transit peptide
Ligand	NAD
Molecular function	Oxidoreductase
PTM	Acetylation
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	ethanol catabolic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	aldehyde dehydrogenase (NAD) activity Inferred from electronic annotation. Source: UniProtKB-EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	‹1 – 11	›11	Mitochondrion Potential
	Chain	12 – 511	500	Aldehyde dehydrogenase X, mitochondrial		PRO_0000056477
Regions
	Nucleotide binding	256 – 261	6	NAD By similarity
Sites
	Active site	279	1	Proton acceptor By similarity
	Active site	313	1	Nucleophile By similarity
	Site	180	1	Transition state stabilizer By similarity
Amino acid modifications
	Modified residue	45	1	N6-acetyllysine By similarity
	Modified residue	46	1	N6-acetyllysine By similarity
	Modified residue	377	1	N6-acetyllysine By similarity
	Modified residue	393	1	N6-acetyllysine By similarity
	Modified residue	420	1	N6-acetyllysine By similarity
	Modified residue	423	1	N6-acetyllysine By similarity
Experimental info
	Sequence conflict	308	1	N → S in AAB26659. Ref.2
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P52476 [UniParc]. Last modified January 9, 2007. Version 2. Checksum: D032C6E84D9799B3	FASTA	511	56,770
	10 20 30 40 50 60 PRLFALHHSA TQYFSAAALP SPIPNPDIPD NQLFISNKWH DAVSKKTFPT VSPATGEVIG 70 80 90 100 110 120 HVAEGDWADV DLAAKAARAA FRLGSPWRWM DALKRGWLLN HLADLVERDC VYLASLESLD 130 140 150 160 170 180 NGKPFQESYV LDLDEVIKVY RYFAGWADKW HGKTIPMDGE HFCFTRHEPV GVCCQIIPWN 190 200 210 220 230 240 FPLVMQSWKL ALALAMGNTV VTKVAEQTPF SALYLASLIK EVGLPPGLVN IVTGYGPTAG 250 260 270 280 290 300 AAIAHHMDIG KVAFTGSTKV GHLIQKAAGN SSLKRVTLEL GGKSLSIVLA DADMDHAVEQ 310 320 330 340 350 360 RQEALFFNMG QCCCPGSWTF IEESIYDEFL ERTVEKAKQR RVGNPFDLDT QQGPQVDRER 370 380 390 400 410 420 FERILGYIQL GQKEGAKLLC GGEHFRQQCF FIKPTVFGGV QDDMRIAREE IFGPVQPLFK 430 440 450 460 470 480 FKKIEEVIER ADNTRYGLAA AVFTQDLDKA MYFTQALQTG TVWVNTYNVV TCHTPLGGFK 490 500 510 EPGNGRELGE DGLKAYTEVK TVTIKVPQKN S

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The genome sequence of taurine cattle: a window to ruminant biology and evolution." The bovine genome sequencing and analysis consortium Science 324:522-528(2009) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Hereford.
[2]	"Bovine corneal aldehyde dehydrogenases: evidence for multiple gene products (ALDH3 and ALDHX)." Algar E.M., Cheung B., Hayes J., Holmes R.S., Beacham I.R. Adv. Exp. Med. Biol. 328:153-157(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 284-409. Tissue: Cornea.

Cross-references

Sequence databases
EMBL GenBank DDBJ	AAFC03050684 Genomic DNA. No translation available. S61045 mRNA. Translation: AAB26659.1.
IPI	IPI00908070.
PIR	I46935.
UniGene	Bt.13145.
3D structure databases
ProteinModelPortal	P52476.
SMR	P52476. Positions 18-511.
ModBase	Search...
Protein-protein interaction databases
STRING	9913.ENSBTAP00000027172.
Proteomic databases
PRIDE	P52476.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Phylogenomic databases
eggNOG	COG1012.
HOGENOM	HOG000271505.
HOVERGEN	HBG000097.
InParanoid	P52476.
Enzyme and pathway databases
UniPathway	UPA00780; UER00768.
Gene expression databases
ArrayExpress	P52476.
Family and domain databases
Gene3D	3.40.309.10. 1 hit. 3.40.605.10. 1 hit.
InterPro	IPR016161. Ald_DH/histidinol_DH. IPR016163. Ald_DH_C. IPR016162. Ald_DH_N. IPR015590. Aldehyde_DH_dom. [Graphical view]
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
SUPFAM	SSF53720. SSF53720. 1 hit.
PROSITE	PS00070. ALDEHYDE_DEHYDR_CYS. False negative. PS00687. ALDEHYDE_DEHYDR_GLU. False negative. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

AL1B1_BOVIN

Accession

Primary (citable) accession number: P52476

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 9, 2007
Last modified:	December 11, 2013
This is version 96 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program