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P52435

- RPB11_HUMAN

UniProt

P52435 - RPB11_HUMAN

Protein

DNA-directed RNA polymerase II subunit RPB11-a

Gene

POLR2J

Organism
Homo sapiens (Human)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
  1. Functioni

    DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase II which synthesizes mRNA precursors and many functional non-coding RNAs. Pol II is the central component of the basal RNA polymerase II transcription machinery. It is composed of mobile elements that move relative to each other. RPB11 is part of the core element with the central large cleft By similarity.By similarity

    GO - Molecular functioni

    1. DNA binding Source: InterPro
    2. DNA-directed RNA polymerase activity Source: ProtInc
    3. LRR domain binding Source: UniProtKB
    4. protein binding Source: UniProtKB

    GO - Biological processi

    1. 7-methylguanosine mRNA capping Source: Reactome
    2. DNA repair Source: Reactome
    3. gene expression Source: Reactome
    4. mRNA splicing, via spliceosome Source: Reactome
    5. nucleotide-excision repair Source: Reactome
    6. positive regulation of viral transcription Source: Reactome
    7. RNA splicing Source: Reactome
    8. transcription-coupled nucleotide-excision repair Source: Reactome
    9. transcription elongation from RNA polymerase II promoter Source: Reactome
    10. transcription from RNA polymerase II promoter Source: UniProtKB
    11. transcription initiation from RNA polymerase II promoter Source: Reactome
    12. viral process Source: Reactome

    Keywords - Biological processi

    Transcription

    Enzyme and pathway databases

    ReactomeiREACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    DNA-directed RNA polymerase II subunit RPB11-a
    Short name:
    RNA polymerase II subunit B11-a
    Short name:
    RPB11a
    Alternative name(s):
    DNA-directed RNA polymerase II subunit J-1
    RNA polymerase II 13.3 kDa subunit
    Gene namesi
    Name:POLR2J
    Synonyms:POLR2J1
    OrganismiHomo sapiens (Human)
    Taxonomic identifieri9606 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
    ProteomesiUP000005640: Chromosome 7

    Organism-specific databases

    HGNCiHGNC:9197. POLR2J.

    Subcellular locationi

    Nucleus 1 Publication

    GO - Cellular componenti

    1. DNA-directed RNA polymerase II, core complex Source: UniProtKB
    2. nucleoplasm Source: Reactome
    3. nucleus Source: UniProtKB

    Keywords - Cellular componenti

    DNA-directed RNA polymerase, Nucleus

    Pathology & Biotechi

    Organism-specific databases

    PharmGKBiPA33517.

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 117117DNA-directed RNA polymerase II subunit RPB11-aPRO_0000149309Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionine1 Publication

    Keywords - PTMi

    Acetylation

    Proteomic databases

    MaxQBiP52435.
    PaxDbiP52435.
    PRIDEiP52435.

    PTM databases

    PhosphoSiteiP52435.

    Expressioni

    Tissue specificityi

    Ubiquitously expressed. High expression was found in heart and skeletal muscle.1 Publication

    Gene expression databases

    ArrayExpressiP52435.
    BgeeiP52435.
    CleanExiHS_POLR2J.
    GenevestigatoriP52435.

    Organism-specific databases

    HPAiHPA044010.
    HPA046970.

    Interactioni

    Subunit structurei

    Component of the RNA polymerase II (Pol II) complex consisting of 12 subunits. Interacts with AATF.2 Publications

    Protein-protein interaction databases

    BioGridi111435. 40 interactions.
    DIPiDIP-32913N.
    IntActiP52435. 12 interactions.
    MINTiMINT-131416.
    STRINGi9606.ENSP00000292614.

    Structurei

    3D structure databases

    ProteinModelPortaliP52435.
    SMRiP52435. Positions 1-105.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Phylogenomic databases

    eggNOGiCOG1761.
    HOGENOMiHOG000179862.
    HOVERGENiHBG073419.
    KOiK03008.
    OrthoDBiEOG7GN2P7.
    PhylomeDBiP52435.
    TreeFamiTF103044.

    Family and domain databases

    InterProiIPR009025. RBP11-like_dimer.
    IPR008193. RNA_pol_Rpb11_13-16kDa_CS.
    [Graphical view]
    SUPFAMiSSF55257. SSF55257. 1 hit.
    PROSITEiPS01154. RNA_POL_L_13KD. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52435-1 [UniParc]FASTAAdd to Basket

    « Hide

    MNAPPAFESF LLFEGEKKIT INKDTKVPNA CLFTINKEDH TLGNIIKSQL    50
    LKDPQVLFAG YKVPHPLEHK IIIRVQTTPD YSPQEAFTNA ITDLISELSL 100
    LEERFRVAIK DKQEGIE 117
    Length:117
    Mass (Da):13,293
    Last modified:October 1, 1996 - v1
    Checksum:i6F78AB256EF8F647
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82385 mRNA. Translation: CAA57785.1.
    L37127 mRNA. Translation: AAD05361.1.
    X98433 mRNA. Translation: CAA67075.1.
    AK290515 mRNA. Translation: BAF83204.1.
    AC093668 Genomic DNA. No translation available.
    BC024165 mRNA. Translation: AAH24165.1.
    BC065711 mRNA. Translation: AAH65711.1.
    BC139902 mRNA. Translation: AAI39903.1.
    BC141830 mRNA. Translation: AAI41831.1.
    CCDSiCCDS5724.1.
    PIRiS71325.
    RefSeqiNP_006225.1. NM_006234.4.
    UniGeneiHs.654952.

    Genome annotation databases

    EnsembliENST00000292614; ENSP00000292614; ENSG00000005075.
    GeneIDi5439.
    KEGGihsa:5439.
    UCSCiuc003uzp.1. human.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X82385 mRNA. Translation: CAA57785.1 .
    L37127 mRNA. Translation: AAD05361.1 .
    X98433 mRNA. Translation: CAA67075.1 .
    AK290515 mRNA. Translation: BAF83204.1 .
    AC093668 Genomic DNA. No translation available.
    BC024165 mRNA. Translation: AAH24165.1 .
    BC065711 mRNA. Translation: AAH65711.1 .
    BC139902 mRNA. Translation: AAI39903.1 .
    BC141830 mRNA. Translation: AAI41831.1 .
    CCDSi CCDS5724.1.
    PIRi S71325.
    RefSeqi NP_006225.1. NM_006234.4.
    UniGenei Hs.654952.

    3D structure databases

    ProteinModelPortali P52435.
    SMRi P52435. Positions 1-105.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    BioGridi 111435. 40 interactions.
    DIPi DIP-32913N.
    IntActi P52435. 12 interactions.
    MINTi MINT-131416.
    STRINGi 9606.ENSP00000292614.

    PTM databases

    PhosphoSitei P52435.

    Proteomic databases

    MaxQBi P52435.
    PaxDbi P52435.
    PRIDEi P52435.

    Protocols and materials databases

    DNASUi 5439.
    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENST00000292614 ; ENSP00000292614 ; ENSG00000005075 .
    GeneIDi 5439.
    KEGGi hsa:5439.
    UCSCi uc003uzp.1. human.

    Organism-specific databases

    CTDi 5439.
    GeneCardsi GC07M102113.
    H-InvDB HIX0025314.
    HGNCi HGNC:9197. POLR2J.
    HPAi HPA044010.
    HPA046970.
    MIMi 604150. gene.
    neXtProti NX_P52435.
    PharmGKBi PA33517.
    GenAtlasi Search...

    Phylogenomic databases

    eggNOGi COG1761.
    HOGENOMi HOG000179862.
    HOVERGENi HBG073419.
    KOi K03008.
    OrthoDBi EOG7GN2P7.
    PhylomeDBi P52435.
    TreeFami TF103044.

    Enzyme and pathway databases

    Reactomei REACT_12417. MicroRNA (miRNA) biogenesis.
    REACT_125. Processing of Capped Intron-Containing Pre-mRNA.
    REACT_1470. mRNA Capping.
    REACT_1628. Transcription-coupled NER (TC-NER).
    REACT_1655. RNA Polymerase II Transcription Pre-Initiation And Promoter Opening.
    REACT_1753. mRNA Splicing - Minor Pathway.
    REACT_1851. RNA Polymerase II Transcription Initiation.
    REACT_1941. Formation of transcription-coupled NER (TC-NER) repair complex.
    REACT_2089. RNA Polymerase II Promoter Escape.
    REACT_22107. RNA Polymerase II Pre-transcription Events.
    REACT_22201. Formation of HIV elongation complex in the absence of HIV Tat.
    REACT_2222. Dual incision reaction in TC-NER.
    REACT_467. mRNA Splicing - Major Pathway.
    REACT_6143. Pausing and recovery of Tat-mediated HIV elongation.
    REACT_6162. Tat-mediated elongation of the HIV-1 transcript.
    REACT_6233. Transcription of the HIV genome.
    REACT_6237. RNA Pol II CTD phosphorylation and interaction with CE.
    REACT_6244. Pausing and recovery of HIV elongation.
    REACT_6253. RNA Polymerase II HIV Promoter Escape.
    REACT_6259. HIV elongation arrest and recovery.
    REACT_6261. Abortive elongation of HIV-1 transcript in the absence of Tat.
    REACT_6319. Formation of the HIV-1 Early Elongation Complex.
    REACT_6332. HIV Transcription Initiation.
    REACT_6344. Tat-mediated HIV elongation arrest and recovery.
    REACT_6346. Formation of HIV-1 elongation complex containing HIV-1 Tat.
    REACT_6354. Viral Messenger RNA Synthesis.
    REACT_833. RNA Polymerase II Transcription Elongation.
    REACT_834. RNA Polymerase II Transcription Initiation And Promoter Clearance.
    REACT_846. Formation of the Early Elongation Complex.
    REACT_975. RNA Pol II CTD phosphorylation and interaction with CE.

    Miscellaneous databases

    ChiTaRSi POLR2J. human.
    GeneWikii POLR2J.
    GenomeRNAii 5439.
    NextBioi 21045.
    PROi P52435.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52435.
    Bgeei P52435.
    CleanExi HS_POLR2J.
    Genevestigatori P52435.

    Family and domain databases

    InterProi IPR009025. RBP11-like_dimer.
    IPR008193. RNA_pol_Rpb11_13-16kDa_CS.
    [Graphical view ]
    SUPFAMi SSF55257. SSF55257. 1 hit.
    PROSITEi PS01154. RNA_POL_L_13KD. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Cloning of a novel human RNA polymerase II subunit downregulated by doxorubicin: new potential mechanisms of drug related toxicity."
      Fanciulli M., Bruno T., Cerboni C., Bonetto F., Iacobini C., Frati L., Piccoli M., Floridi A., Santoni A., Punturieri A.
      FEBS Lett. 384:48-52(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Colon.
    2. Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    3. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
      Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
      , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
      Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Brain.
    4. "The DNA sequence of human chromosome 7."
      Hillier L.W., Fulton R.S., Fulton L.A., Graves T.A., Pepin K.H., Wagner-McPherson C., Layman D., Maas J., Jaeger S., Walker R., Wylie K., Sekhon M., Becker M.C., O'Laughlin M.D., Schaller M.E., Fewell G.A., Delehaunty K.D., Miner T.L.
      , Nash W.E., Cordes M., Du H., Sun H., Edwards J., Bradshaw-Cordum H., Ali J., Andrews S., Isak A., Vanbrunt A., Nguyen C., Du F., Lamar B., Courtney L., Kalicki J., Ozersky P., Bielicki L., Scott K., Holmes A., Harkins R., Harris A., Strong C.M., Hou S., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Leonard S., Rohlfing T., Rock S.M., Tin-Wollam A.-M., Abbott A., Minx P., Maupin R., Strowmatt C., Latreille P., Miller N., Johnson D., Murray J., Woessner J.P., Wendl M.C., Yang S.-P., Schultz B.R., Wallis J.W., Spieth J., Bieri T.A., Nelson J.O., Berkowicz N., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Bedell J.A., Mardis E.R., Clifton S.W., Chissoe S.L., Marra M.A., Raymond C., Haugen E., Gillett W., Zhou Y., James R., Phelps K., Iadanoto S., Bubb K., Simms E., Levy R., Clendenning J., Kaul R., Kent W.J., Furey T.S., Baertsch R.A., Brent M.R., Keibler E., Flicek P., Bork P., Suyama M., Bailey J.A., Portnoy M.E., Torrents D., Chinwalla A.T., Gish W.R., Eddy S.R., McPherson J.D., Olson M.V., Eichler E.E., Green E.D., Waterston R.H., Wilson R.K.
      Nature 424:157-164(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    5. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Tissue: Bone marrow and Kidney.
    6. Bienvenut W.V., Waridel P., Quadroni M.
      Submitted (MAR-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 1-18; 27-37; 48-62 AND 75-104, ACETYLATION AT MET-1, IDENTIFICATION BY MASS SPECTROMETRY.
      Tissue: Embryonic kidney.
    7. "Immunoaffinity purification and functional characterization of human transcription factor IIH and RNA polymerase II from clonal cell lines that conditionally express epitope-tagged subunits of the multiprotein complexes."
      Kershnar E., Wu S.-Y., Chiang C.-M.
      J. Biol. Chem. 273:34444-34453(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: FUNCTION, IDENTIFICATION IN THE RNA POLYMERASE II CORE-COMPLEX, SUBCELLULAR LOCATION.
    8. "Identification of a novel partner of RNA polymerase II subunit 11, Che-1, which interacts with and affects the growth suppression function of Rb."
      Fanciulli M., Bruno T., Di Padova M., De Angelis R., Iezzi S., Iacobini C., Floridi A., Passananti C.
      FASEB J. 14:904-912(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH AATF.
    9. "A human RNA polymerase II subunit is encoded by a recently generated multigene family."
      Grandemange S., Schaller S., Yamano S., Du Manoir S., Shpakovski G.V., Mattei M.-G., Kedinger C., Vigneron M.
      BMC Mol. Biol. 2:14-14(2001) [PubMed] [Europe PMC] [Abstract]
      Cited for: TISSUE SPECIFICITY.

    Entry informationi

    Entry nameiRPB11_HUMAN
    AccessioniPrimary (citable) accession number: P52435
    Secondary accession number(s): A5D6V8, O43375
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program
    DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. Human chromosome 7
      Human chromosome 7: entries, gene names and cross-references to MIM
    2. MIM cross-references
      Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3