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Protein

DNA-directed RNA polymerases I and III subunit RPAC1

Gene

Polr1c

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Common component of RNA polymerases I and III which synthesize ribosomal RNA precursors and small RNAs, such as 5S rRNA and tRNAs, respectively. RPAC1 is part of the Pol core element with the central large cleft and probably a clamp element that moves to open and close the cleft (By similarity).By similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiR-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerases I and III subunit RPAC1
Short name:
DNA-directed RNA polymerase I subunit C
Short name:
RNA polymerases I and III subunit AC1
Alternative name(s):
AC40
DNA-directed RNA polymerases I and III 40 kDa polypeptide
Short name:
RPA40
RPC40
Gene namesi
Name:Polr1c
Synonyms:Rpo1-1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Chromosome 17

Organism-specific databases

MGIiMGI:103288. Polr1c.

Subcellular locationi

  • Nucleus By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi73 – 731A → D: Unable to interact with RPB14. 1 Publication
Mutagenesisi73 – 731A → R: Temperature-sensitive defect in the interaction with RPB14. 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methionineiRemovedBy similarity
Chaini2 – 346345DNA-directed RNA polymerases I and III subunit RPAC1PRO_0000132740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei2 – 21N-acetylalanineBy similarity

Keywords - PTMi

Acetylation

Proteomic databases

EPDiP52432.
MaxQBiP52432.
PaxDbiP52432.
PeptideAtlasiP52432.
PRIDEiP52432.

PTM databases

iPTMnetiP52432.
PhosphoSiteiP52432.

Expressioni

Gene expression databases

BgeeiP52432.
CleanExiMM_POLR1E.
ExpressionAtlasiP52432. baseline and differential.
GenevisibleiP52432. MM.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) and RNA polymerase III (Pol III) complexes consisting of at least 13 and 17 subunits, respectively.By similarity

Protein-protein interaction databases

IntActiP52432. 1 interaction.
MINTiMINT-4132992.
STRINGi10090.ENSMUSP00000084252.

Structurei

3D structure databases

ProteinModelPortaliP52432.
SMRiP52432. Positions 40-332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1521. Eukaryota.
COG0202. LUCA.
GeneTreeiENSGT00550000075060.
HOVERGENiHBG017743.
InParanoidiP52432.
KOiK03027.
OMAiGKCQRFL.
OrthoDBiEOG7X6M08.
TreeFamiTF103034.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52432-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAAQAVEEM RTRVVLGEFG VRNVHTTDFP GNYAGYDDAW DQNRFEKNFR
60 70 80 90 100
VDVVQMDEDT LEFDMVGIDA AIANAFRRIL LAEVPTMAVE KVLVYNNTSI
110 120 130 140 150
VQDEILAHRL GLIPILADPR LFEYRNQGEE EGTEIDTLQF RLQVRCTRNP
160 170 180 190 200
NAAKDSSDPN ELYVNHKVYT RHMTWVPLGN QADVFPEGTI RPVHDDILIA
210 220 230 240 250
QLRPGQEIDL MMHCVKGIGK DHAKFSPVAT ASYRLLPAIT LLEPVEGEAA
260 270 280 290 300
EELSQCFSPG VIEVEEVQGK KVARVANARL DTFSREIFRH EKLKKAVRLA
310 320 330 340
RVRDHYIFSV ESTGVLPPDV LVSEAIKILM GKCRRFLDEL DAVEMD
Length:346
Mass (Da):39,107
Last modified:July 27, 2011 - v3
Checksum:iC2115C1526974344
GO

Sequence cautioni

The sequence BAA06735.1 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti52 – 521D → G in BAA06735 (PubMed:7929437).Curated
Sequence conflicti83 – 831E → R in BAA06735 (PubMed:7929437).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31966 mRNA. Translation: BAA06735.1. Different initiation.
AK132114 mRNA. Translation: BAE20986.1.
AK146240 mRNA. Translation: BAE27005.1.
CH466559 Genomic DNA. Translation: EDL23483.1.
CCDSiCCDS37632.1.
PIRiA55082.
RefSeqiNP_033111.2. NM_009085.2.
UniGeneiMm.3458.

Genome annotation databases

EnsembliENSMUST00000087026; ENSMUSP00000084252; ENSMUSG00000067148.
GeneIDi20016.
KEGGimmu:20016.
UCSCiuc008csc.1. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D31966 mRNA. Translation: BAA06735.1. Different initiation.
AK132114 mRNA. Translation: BAE20986.1.
AK146240 mRNA. Translation: BAE27005.1.
CH466559 Genomic DNA. Translation: EDL23483.1.
CCDSiCCDS37632.1.
PIRiA55082.
RefSeqiNP_033111.2. NM_009085.2.
UniGeneiMm.3458.

3D structure databases

ProteinModelPortaliP52432.
SMRiP52432. Positions 40-332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52432. 1 interaction.
MINTiMINT-4132992.
STRINGi10090.ENSMUSP00000084252.

PTM databases

iPTMnetiP52432.
PhosphoSiteiP52432.

Proteomic databases

EPDiP52432.
MaxQBiP52432.
PaxDbiP52432.
PeptideAtlasiP52432.
PRIDEiP52432.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000087026; ENSMUSP00000084252; ENSMUSG00000067148.
GeneIDi20016.
KEGGimmu:20016.
UCSCiuc008csc.1. mouse.

Organism-specific databases

CTDi9533.
MGIiMGI:103288. Polr1c.

Phylogenomic databases

eggNOGiKOG1521. Eukaryota.
COG0202. LUCA.
GeneTreeiENSGT00550000075060.
HOVERGENiHBG017743.
InParanoidiP52432.
KOiK03027.
OMAiGKCQRFL.
OrthoDBiEOG7X6M08.
TreeFamiTF103034.

Enzyme and pathway databases

ReactomeiR-MMU-1834949. Cytosolic sensors of pathogen-associated DNA.
R-MMU-427413. NoRC negatively regulates rRNA expression.
R-MMU-5250924. B-WICH complex positively regulates rRNA expression.
R-MMU-5601884. PIWI-interacting RNA (piRNA) biogenesis.
R-MMU-5617472. Activation of anterior HOX genes in hindbrain development during early embryogenesis.
R-MMU-573389. NoRC negatively regulates rRNA expression.
R-MMU-73762. RNA Polymerase I Transcription Initiation.
R-MMU-73772. RNA Polymerase I Promoter Escape.
R-MMU-73777. RNA Polymerase I Chain Elongation.
R-MMU-73863. RNA Polymerase I Transcription Termination.
R-MMU-76061. RNA Polymerase III Transcription Initiation From Type 1 Promoter.
R-MMU-76066. RNA Polymerase III Transcription Initiation From Type 2 Promoter.
R-MMU-76071. RNA Polymerase III Transcription Initiation From Type 3 Promoter.

Miscellaneous databases

PROiP52432.
SOURCEiSearch...

Gene expression databases

BgeeiP52432.
CleanExiMM_POLR1E.
ExpressionAtlasiP52432. baseline and differential.
GenevisibleiP52432. MM.

Family and domain databases

Gene3Di2.170.120.12. 1 hit.
InterProiIPR001514. DNA-dir_RNA_pol_30-40kDasu_CS.
IPR011262. DNA-dir_RNA_pol_insert.
IPR011263. DNA-dir_RNA_pol_RpoA/D/Rpb3.
IPR009025. RBP11-like_dimer.
[Graphical view]
PfamiPF01000. RNA_pol_A_bac. 1 hit.
PF01193. RNA_pol_L. 1 hit.
[Graphical view]
SMARTiSM00662. RPOLD. 1 hit.
[Graphical view]
SUPFAMiSSF55257. SSF55257. 2 hits.
SSF56553. SSF56553. 1 hit.
PROSITEiPS00446. RNA_POL_D_30KD. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "High conservation of subunit composition of RNA polymerase I(A) between yeast and mouse and the molecular cloning of mouse RNA polymerase I 40-kDa subunit RPA40."
    Song C.Z., Hanada K., Yano K., Maeda Y., Yamamoto K., Muramatsu M.
    J. Biol. Chem. 269:26976-26981(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The transcriptional landscape of the mammalian genome."
    Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
    , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
    Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: C57BL/6J and DBA/2.
    Tissue: Head.
  3. Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.
    Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "Isolation and characterization of cDNA encoding mouse RNA polymerase II subunit RPB14."
    Nishi Y., Yamamoto K., Yao Y., Yamamoto M., Nogi Y., Matsuo H., Muramatsu M.
    Gene 187:165-170(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: MUTAGENESIS OF ALA-73.
  5. Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Spleen and Testis.

Entry informationi

Entry nameiRPAC1_MOUSE
AccessioniPrimary (citable) accession number: P52432
Secondary accession number(s): Q3UJZ9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: July 27, 2011
Last modified: July 6, 2016
This is version 133 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.