Reviewed, UniProtKB/Swiss-Prot P52430 (PON1_MOUSE)

Names and origin

Protein names

Recommended name:
    Serum paraoxonase/arylesterase 1
      Short name=PON 1
    EC=3.1.1.2
    EC=3.1.8.1
Alternative name(s):
    Serum aryldialkylphosphatase 1
    Aromatic esterase 1
      Short name=A-esterase 1

Gene names

Name:	Pon1
Synonyms:	Pon

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	355 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and a number of aromatic carboxylic acid esters.
Catalytic activity	A phenyl acetate + H2O = a phenol + acetate. An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.
Cofactor	Binds 2 calcium ions per subunit By similarity.
Subunit structure	Interacts with CLU By similarity.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.
Post-translational modification	The signal sequence is not cleaved By similarity.
Miscellaneous	The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces By similarity.
Sequence similarities	Belongs to the paraoxonase family.

Ontologies

Keywords
Cellular component	HDL Secreted
Domain	Signal
Ligand	Calcium Metal-binding
Molecular function	Hydrolase
PTM	Disulfide bond Glycoprotein Phosphoprotein
Gene Ontology (GO)
Biological process	blood circulation Traceable author statement. Source: MGI cholesterol metabolic process Inferred from mutant phenotype. Source: MGI response to toxin Inferred from mutant phenotype. Source: MGI
Molecular function	aryldialkylphosphatase activity Inferred from sequence or structural similarity. Source: UniProtKB arylesterase activity Inferred from sequence or structural similarity. Source: UniProtKB calcium ion binding Inferred from sequence or structural similarity. Source: UniProtKB high-density lipoprotein binding Traceable author statement. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – ?		Not cleaved
	Initiator methionine	1	1	Removed By similarity
	Chain	2 – 355	354	Serum paraoxonase/arylesterase 1		PRO_0000223282
Sites
	Active site	115	1	Proton acceptor By similarity
	Metal binding	53	1	Calcium 1; catalytic By similarity
	Metal binding	54	1	Calcium 2 By similarity
	Metal binding	117	1	Calcium 2; via carbonyl oxygen By similarity
	Metal binding	168	1	Calcium 1; catalytic By similarity
	Metal binding	169	1	Calcium 2 By similarity
	Metal binding	224	1	Calcium 1; catalytic By similarity
	Metal binding	269	1	Calcium 1; catalytic By similarity
	Metal binding	270	1	Calcium 1; catalytic By similarity
Amino acid modifications
	Modified residue	76	1	Phosphoserine By similarity
	Modified residue	80	1	Phosphoserine By similarity
	Glycosylation	253	1	N-linked (GlcNAc...) Ref.4
	Glycosylation	270	1	N-linked (GlcNAc...) Potential
	Glycosylation	324	1	N-linked (GlcNAc...) Potential
	Disulfide bond	42 ↔ 353		By similarity
Experimental info
	Sequence conflict	186	1	F → L in AAC52496. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	P52430-1 [UniParc]. Last modified January 23, 2007. Version 2. Checksum: FFA0A71445BB80B4	FASTA	355	39,565
	10 20 30 40 50 60 MAKLLALTLV GLVLALYKNH RSSYQTRLNA FREVTPVELP NCNLVKGIET GAEDLEILPN 70 80 90 100 110 120 GLTFFSTGLK YPGIKSFDPS KPGKILLMDL NKKEPAVSEL EIIGNTLDIS SFNPHGISTF 130 140 150 160 170 180 TDEDNTVYLL VVNHPDSSST VEVFKFQEEE RSLLHLKTIT HELLPSINDI AAIGPESFYA 190 200 210 220 230 240 TNDHYFADPY LRSWEMYLGL SWSNVVYYSP DKVQVVAEGF DFANGIGISL DGKYVYIAEL 250 260 270 280 290 300 LAHKIHVYEK HANWTLTPLK VLNFDTLVDN ISVDPVTGDL WVGCHPNGMR IFFYDAENPP 310 320 330 340 350 GSEVLRIQNI LSEDPKITVV YAENGTVLQG TTVASVYKGK LLIGTVFHKA LYCDL

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Reconsideration of the catalytic center and mechanism of mammalian paraoxonase/arylesterase." Sorenson R.C., Primo-Parmo S.L., Kuo C.-L., Adkins S., Lockridge O., La Du B.N. Proc. Natl. Acad. Sci. U.S.A. 92:7187-7191(1995) [PubMed: 7638166] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: BALB/c. Tissue: Liver.
[2]	"Genetic-dietary regulation of serum paraoxonase expression and its role in atherogenesis in a mouse model." Shih D.M., Gu L., Hama S., Xia Y.R., Navab M., Fogelman A.M., Lusis A.J. J. Clin. Invest. 97:1630-1639(1996) [PubMed: 8601628] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: C57BL/6 X CBA. Tissue: Liver.
[3]	"Paraoxonase (PON1) gene in mice: sequencing, chromosomal localization and developmental expression." Li W.F., Matthews C., Disteche C.M., Costa L.G., Furlong C.E. Pharmacogenetics 7:137-144(1997) [PubMed: 9170151] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: BALB/c.
[4]	"Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides." Bernhard O.K., Kapp E.A., Simpson R.J. J. Proteome Res. 6:987-995(2007) [PubMed: 17330941] [Abstract] Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-253, MASS SPECTROMETRY. Tissue: Plasma.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L40488 Genomic DNA. Translation: AAA99445.1. U32684 mRNA. Translation: AAC52496.1. U72636 mRNA. Translation: AAB17394.1.
IPI	IPI00317356.
PIR	T10082.
RefSeq	NP_035264.2.
UniGene	Mm.237657
3D structure databases
SMR	P52430. Positions 16-355.
ModBase	Search...
Protein-protein interaction databases
STRING	P52430.
Proteomic databases
PRIDE	P52430.
Genome annotation databases
Ensembl	ENSMUST00000002663; ENSMUSP00000002663; ENSMUSG00000002588; Mus musculus. [Genome view]
GeneID	18979.
KEGG	mmu:18979.
UCSC	uc009awd.1. mouse.
Organism-specific databases
CTD	18979.
MGI	MGI:103295. Pon1.
Phylogenomic databases
eggNOG	roNOG04341.
HOGENOM	HBG613410.
HOVERGEN	P52430.
InParanoid	P52430.
OMA	TNDHYFV.
OrthoDB	EOG912PQX.
PhylomeDB	P52430.
Enzyme and pathway databases
BRENDA	3.1.1.2. 244. 3.1.8.1. 244.
Gene expression databases
ArrayExpress	P52430.
Bgee	P52430.
CleanEx	MM_PON1.
Genevestigator	P52430.
GermOnline	ENSMUSG00000002588. Mus musculus.
Family and domain databases
InterPro	IPR011042. 6-blade_b-propeller_TolB-like. IPR002640. Arylesterase. IPR008363. Paraoxonase1. [Graphical view]
Gene3D	G3DSA:2.120.10.30. 6-blade_b-propeller_TolB-like. 1 hit.
Pfam	PF01731. Arylesterase. 1 hit. [Graphical view]
PRINTS	PR01785. PARAOXONASE. PR01786. PARAOXONASE1.
ProtoNet	Search...
Other Resources
NextBio	295346.
SOURCE	Search...

Entry information

Entry name

PON1_MOUSE

Accession

Primary (citable) accession number: P52430

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 23, 2007
Last modified:	February 9, 2010
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)