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P52430

- PON1_MOUSE

UniProt

P52430 - PON1_MOUSE

Protein

Serum paraoxonase/arylesterase 1

Gene

Pon1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 117 (01 Oct 2014)
      Sequence version 2 (23 Jan 2007)
      Previous versions | rss
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    Functioni

    Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification By similarity.By similarity

    Catalytic activityi

    A phenyl acetate + H2O = a phenol + acetate.1 Publication
    An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.1 Publication
    An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine.1 Publication

    Cofactori

    Binds 2 calcium ions per subunit.By similarity

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Metal bindingi53 – 531Calcium 1; catalyticBy similarity
    Metal bindingi54 – 541Calcium 2By similarity
    Active sitei115 – 1151Proton acceptorBy similarity
    Metal bindingi117 – 1171Calcium 2; via carbonyl oxygenBy similarity
    Metal bindingi168 – 1681Calcium 1; catalyticBy similarity
    Metal bindingi169 – 1691Calcium 2By similarity
    Metal bindingi224 – 2241Calcium 1; catalyticBy similarity
    Metal bindingi269 – 2691Calcium 1; catalyticBy similarity
    Metal bindingi270 – 2701Calcium 1; catalyticBy similarity

    GO - Molecular functioni

    1. aryldialkylphosphatase activity Source: UniProtKB
    2. arylesterase activity Source: UniProtKB
    3. calcium ion binding Source: UniProtKB
    4. high-density lipoprotein particle binding Source: MGI
    5. phospholipid binding Source: Ensembl

    GO - Biological processi

    1. aromatic compound catabolic process Source: Ensembl
    2. blood circulation Source: MGI
    3. carboxylic acid catabolic process Source: Ensembl
    4. cholesterol metabolic process Source: MGI
    5. dephosphorylation Source: GOC
    6. organophosphate catabolic process Source: Ensembl
    7. phosphatidylcholine metabolic process Source: Ensembl
    8. positive regulation of binding Source: Ensembl
    9. positive regulation of cholesterol efflux Source: Ensembl
    10. positive regulation of transporter activity Source: Ensembl
    11. response to toxic substance Source: MGI

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Ligandi

    Calcium, Metal-binding

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Serum paraoxonase/arylesterase 1 (EC:3.1.1.2, EC:3.1.1.81, EC:3.1.8.1)
    Short name:
    PON 1
    Alternative name(s):
    Aromatic esterase 1
    Short name:
    A-esterase 1
    Serum aryldialkylphosphatase 1
    Gene namesi
    Name:Pon1
    Synonyms:Pon
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 6

    Organism-specific databases

    MGIiMGI:103295. Pon1.

    Subcellular locationi

    GO - Cellular componenti

    1. intracellular membrane-bounded organelle Source: Ensembl
    2. spherical high-density lipoprotein particle Source: Ensembl

    Keywords - Cellular componenti

    HDL, Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Initiator methioninei1 – 11RemovedBy similarity
    Chaini2 – 355354Serum paraoxonase/arylesterase 1PRO_0000223282Add
    BLAST
    Signal peptidei2 – ?Not cleaved

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Disulfide bondi42 ↔ 353By similarity
    Glycosylationi253 – 2531N-linked (GlcNAc...)1 Publication
    Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
    Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis

    Post-translational modificationi

    The signal sequence is not cleaved.By similarity

    Keywords - PTMi

    Disulfide bond, Glycoprotein

    Proteomic databases

    MaxQBiP52430.
    PaxDbiP52430.
    PRIDEiP52430.

    PTM databases

    PhosphoSiteiP52430.

    Expressioni

    Tissue specificityi

    Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

    Gene expression databases

    ArrayExpressiP52430.
    BgeeiP52430.
    CleanExiMM_PON1.
    GenevestigatoriP52430.

    Interactioni

    Subunit structurei

    Homodimer. Interacts with CLU By similarity.By similarity

    Protein-protein interaction databases

    IntActiP52430. 1 interaction.
    MINTiMINT-4121591.
    STRINGi10090.ENSMUSP00000002663.

    Structurei

    3D structure databases

    ProteinModelPortaliP52430.
    SMRiP52430. Positions 20-355.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the paraoxonase family.Curated

    Keywords - Domaini

    Signal

    Phylogenomic databases

    eggNOGiNOG68009.
    GeneTreeiENSGT00390000008932.
    HOGENOMiHOG000252960.
    HOVERGENiHBG003604.
    InParanoidiQ91X30.
    KOiK01045.
    OMAiNAFREVT.
    OrthoDBiEOG7GN2N3.
    PhylomeDBiP52430.
    TreeFamiTF322436.

    Family and domain databases

    Gene3Di2.120.10.30. 1 hit.
    InterProiIPR011042. 6-blade_b-propeller_TolB-like.
    IPR002640. Arylesterase.
    IPR008363. Paraoxonase1.
    [Graphical view]
    PfamiPF01731. Arylesterase. 1 hit.
    [Graphical view]
    PRINTSiPR01785. PARAOXONASE.
    PR01786. PARAOXONASE1.

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52430-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAKLLALTLV GLVLALYKNH RSSYQTRLNA FREVTPVELP NCNLVKGIET    50
    GAEDLEILPN GLTFFSTGLK YPGIKSFDPS KPGKILLMDL NKKEPAVSEL 100
    EIIGNTLDIS SFNPHGISTF TDEDNTVYLL VVNHPDSSST VEVFKFQEEE 150
    RSLLHLKTIT HELLPSINDI AAIGPESFYA TNDHYFADPY LRSWEMYLGL 200
    SWSNVVYYSP DKVQVVAEGF DFANGIGISL DGKYVYIAEL LAHKIHVYEK 250
    HANWTLTPLK VLNFDTLVDN ISVDPVTGDL WVGCHPNGMR IFFYDAENPP 300
    GSEVLRIQNI LSEDPKITVV YAENGTVLQG TTVASVYKGK LLIGTVFHKA 350
    LYCDL 355
    Length:355
    Mass (Da):39,565
    Last modified:January 23, 2007 - v2
    Checksum:iFFA0A71445BB80B4
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti61 – 611G → E in AAH12706. (PubMed:15489334)Curated
    Sequence conflicti186 – 1861F → L in AAC52496. (PubMed:8601628)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40488 Genomic DNA. Translation: AAA99445.1.
    U32684 mRNA. Translation: AAC52496.1.
    U72636 mRNA. Translation: AAB17394.1.
    BC012706 mRNA. Translation: AAH12706.1.
    CCDSiCCDS19898.1.
    PIRiT10082.
    RefSeqiNP_035264.2. NM_011134.3.
    UniGeneiMm.237657.

    Genome annotation databases

    EnsembliENSMUST00000002663; ENSMUSP00000002663; ENSMUSG00000002588.
    GeneIDi18979.
    KEGGimmu:18979.
    UCSCiuc009awd.2. mouse.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L40488 Genomic DNA. Translation: AAA99445.1 .
    U32684 mRNA. Translation: AAC52496.1 .
    U72636 mRNA. Translation: AAB17394.1 .
    BC012706 mRNA. Translation: AAH12706.1 .
    CCDSi CCDS19898.1.
    PIRi T10082.
    RefSeqi NP_035264.2. NM_011134.3.
    UniGenei Mm.237657.

    3D structure databases

    ProteinModelPortali P52430.
    SMRi P52430. Positions 20-355.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    IntActi P52430. 1 interaction.
    MINTi MINT-4121591.
    STRINGi 10090.ENSMUSP00000002663.

    PTM databases

    PhosphoSitei P52430.

    Proteomic databases

    MaxQBi P52430.
    PaxDbi P52430.
    PRIDEi P52430.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000002663 ; ENSMUSP00000002663 ; ENSMUSG00000002588 .
    GeneIDi 18979.
    KEGGi mmu:18979.
    UCSCi uc009awd.2. mouse.

    Organism-specific databases

    CTDi 5444.
    MGIi MGI:103295. Pon1.

    Phylogenomic databases

    eggNOGi NOG68009.
    GeneTreei ENSGT00390000008932.
    HOGENOMi HOG000252960.
    HOVERGENi HBG003604.
    InParanoidi Q91X30.
    KOi K01045.
    OMAi NAFREVT.
    OrthoDBi EOG7GN2N3.
    PhylomeDBi P52430.
    TreeFami TF322436.

    Miscellaneous databases

    NextBioi 295346.
    PROi P52430.
    SOURCEi Search...

    Gene expression databases

    ArrayExpressi P52430.
    Bgeei P52430.
    CleanExi MM_PON1.
    Genevestigatori P52430.

    Family and domain databases

    Gene3Di 2.120.10.30. 1 hit.
    InterProi IPR011042. 6-blade_b-propeller_TolB-like.
    IPR002640. Arylesterase.
    IPR008363. Paraoxonase1.
    [Graphical view ]
    Pfami PF01731. Arylesterase. 1 hit.
    [Graphical view ]
    PRINTSi PR01785. PARAOXONASE.
    PR01786. PARAOXONASE1.
    ProtoNeti Search...

    Publicationsi

    1. "Reconsideration of the catalytic center and mechanism of mammalian paraoxonase/arylesterase."
      Sorenson R.C., Primo-Parmo S.L., Kuo C.-L., Adkins S., Lockridge O., La Du B.N.
      Proc. Natl. Acad. Sci. U.S.A. 92:7187-7191(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: BALB/c.
      Tissue: Liver.
    2. "Genetic-dietary regulation of serum paraoxonase expression and its role in atherogenesis in a mouse model."
      Shih D.M., Gu L., Hama S., Xia Y.R., Navab M., Fogelman A.M., Lusis A.J.
      J. Clin. Invest. 97:1630-1639(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: C57BL/6 X CBA.
      Tissue: Liver.
    3. "Paraoxonase (PON1) gene in mice: sequencing, chromosomal localization and developmental expression."
      Li W.F., Matthews C., Disteche C.M., Costa L.G., Furlong C.E.
      Pharmacogenetics 7:137-144(1997) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: BALB/c.
    4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: FVB/N.
      Tissue: Liver.
    5. "Quorum quenching enzyme activity is widely conserved in the sera of mammalian species."
      Yang F., Wang L.H., Wang J., Dong Y.H., Hu J.Y., Zhang L.H.
      FEBS Lett. 579:3713-3717(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY.
    6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
      Bernhard O.K., Kapp E.A., Simpson R.J.
      J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
      Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-253.
      Strain: C57BL/6.
      Tissue: Plasma.

    Entry informationi

    Entry nameiPON1_MOUSE
    AccessioniPrimary (citable) accession number: P52430
    Secondary accession number(s): Q91X30
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: January 23, 2007
    Last modified: October 1, 2014
    This is version 117 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Miscellaneous

    The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces By similarity.By similarity

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3