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Protein

Serum paraoxonase/arylesterase 1

Gene

Pon1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification (By similarity).By similarity

Catalytic activityi

A phenyl acetate + H2O = a phenol + acetate.1 Publication
An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.1 Publication
An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine.1 Publication

Cofactori

Ca2+By similarityNote: Binds 2 calcium ions per subunit.By similarity

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Calcium 1; catalyticBy similarity
Metal bindingi54 – 541Calcium 2By similarity
Active sitei115 – 1151Proton acceptorBy similarity
Metal bindingi117 – 1171Calcium 2; via carbonyl oxygenBy similarity
Metal bindingi168 – 1681Calcium 1; catalyticBy similarity
Metal bindingi169 – 1691Calcium 2By similarity
Metal bindingi224 – 2241Calcium 1; catalyticBy similarity
Metal bindingi269 – 2691Calcium 1; catalyticBy similarity
Metal bindingi270 – 2701Calcium 1; catalyticBy similarity

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Enzyme and pathway databases

BRENDAi3.1.1.2. 3474.
3.1.1.25. 3474.
3.1.8.1. 3474.

Names & Taxonomyi

Protein namesi
Recommended name:
Serum paraoxonase/arylesterase 1 (EC:3.1.1.2, EC:3.1.1.81, EC:3.1.8.1)
Short name:
PON 1
Alternative name(s):
Aromatic esterase 1
Short name:
A-esterase 1
Serum aryldialkylphosphatase 1
Gene namesi
Name:Pon1
Synonyms:Pon
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589 Componenti: Chromosome 6

Organism-specific databases

MGIiMGI:103295. Pon1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11RemovedBy similarity
Chaini2 – 355354Serum paraoxonase/arylesterase 1PRO_0000223282Add
BLAST
Signal peptidei2 – ?Not cleaved

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 353By similarity
Glycosylationi253 – 2531N-linked (GlcNAc...)1 Publication
Glycosylationi270 – 2701N-linked (GlcNAc...)Sequence Analysis
Glycosylationi324 – 3241N-linked (GlcNAc...)Sequence Analysis

Post-translational modificationi

The signal sequence is not cleaved.By similarity

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP52430.
PaxDbiP52430.
PRIDEiP52430.

PTM databases

PhosphoSiteiP52430.

Expressioni

Tissue specificityi

Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Gene expression databases

BgeeiP52430.
CleanExiMM_PON1.
ExpressionAtlasiP52430. baseline and differential.
GenevisibleiP52430. MM.

Interactioni

Subunit structurei

Homodimer. Interacts with CLU (By similarity).By similarity

Protein-protein interaction databases

IntActiP52430. 1 interaction.
MINTiMINT-4121591.
STRINGi10090.ENSMUSP00000002663.

Structurei

3D structure databases

ProteinModelPortaliP52430.
SMRiP52430. Positions 20-355.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the paraoxonase family.Curated

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG68009.
GeneTreeiENSGT00390000008932.
HOGENOMiHOG000252960.
HOVERGENiHBG003604.
InParanoidiP52430.
KOiK01045.
OMAiNAFREVT.
OrthoDBiEOG7GN2N3.
PhylomeDBiP52430.
TreeFamiTF322436.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008363. Paraoxonase1.
[Graphical view]
PfamiPF01731. Arylesterase. 1 hit.
[Graphical view]
PRINTSiPR01785. PARAOXONASE.
PR01786. PARAOXONASE1.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52430-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAKLLALTLV GLVLALYKNH RSSYQTRLNA FREVTPVELP NCNLVKGIET
60 70 80 90 100
GAEDLEILPN GLTFFSTGLK YPGIKSFDPS KPGKILLMDL NKKEPAVSEL
110 120 130 140 150
EIIGNTLDIS SFNPHGISTF TDEDNTVYLL VVNHPDSSST VEVFKFQEEE
160 170 180 190 200
RSLLHLKTIT HELLPSINDI AAIGPESFYA TNDHYFADPY LRSWEMYLGL
210 220 230 240 250
SWSNVVYYSP DKVQVVAEGF DFANGIGISL DGKYVYIAEL LAHKIHVYEK
260 270 280 290 300
HANWTLTPLK VLNFDTLVDN ISVDPVTGDL WVGCHPNGMR IFFYDAENPP
310 320 330 340 350
GSEVLRIQNI LSEDPKITVV YAENGTVLQG TTVASVYKGK LLIGTVFHKA

LYCDL
Length:355
Mass (Da):39,565
Last modified:January 23, 2007 - v2
Checksum:iFFA0A71445BB80B4
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611G → E in AAH12706 (PubMed:15489334).Curated
Sequence conflicti186 – 1861F → L in AAC52496 (PubMed:8601628).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40488 Genomic DNA. Translation: AAA99445.1.
U32684 mRNA. Translation: AAC52496.1.
U72636 mRNA. Translation: AAB17394.1.
BC012706 mRNA. Translation: AAH12706.1.
CCDSiCCDS19898.1.
PIRiT10082.
RefSeqiNP_035264.2. NM_011134.3.
UniGeneiMm.237657.

Genome annotation databases

EnsembliENSMUST00000002663; ENSMUSP00000002663; ENSMUSG00000002588.
GeneIDi18979.
KEGGimmu:18979.
UCSCiuc009awd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L40488 Genomic DNA. Translation: AAA99445.1.
U32684 mRNA. Translation: AAC52496.1.
U72636 mRNA. Translation: AAB17394.1.
BC012706 mRNA. Translation: AAH12706.1.
CCDSiCCDS19898.1.
PIRiT10082.
RefSeqiNP_035264.2. NM_011134.3.
UniGeneiMm.237657.

3D structure databases

ProteinModelPortaliP52430.
SMRiP52430. Positions 20-355.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52430. 1 interaction.
MINTiMINT-4121591.
STRINGi10090.ENSMUSP00000002663.

PTM databases

PhosphoSiteiP52430.

Proteomic databases

MaxQBiP52430.
PaxDbiP52430.
PRIDEiP52430.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSMUST00000002663; ENSMUSP00000002663; ENSMUSG00000002588.
GeneIDi18979.
KEGGimmu:18979.
UCSCiuc009awd.2. mouse.

Organism-specific databases

CTDi5444.
MGIiMGI:103295. Pon1.

Phylogenomic databases

eggNOGiNOG68009.
GeneTreeiENSGT00390000008932.
HOGENOMiHOG000252960.
HOVERGENiHBG003604.
InParanoidiP52430.
KOiK01045.
OMAiNAFREVT.
OrthoDBiEOG7GN2N3.
PhylomeDBiP52430.
TreeFamiTF322436.

Enzyme and pathway databases

BRENDAi3.1.1.2. 3474.
3.1.1.25. 3474.
3.1.8.1. 3474.

Miscellaneous databases

NextBioi295346.
PROiP52430.
SOURCEiSearch...

Gene expression databases

BgeeiP52430.
CleanExiMM_PON1.
ExpressionAtlasiP52430. baseline and differential.
GenevisibleiP52430. MM.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008363. Paraoxonase1.
[Graphical view]
PfamiPF01731. Arylesterase. 1 hit.
[Graphical view]
PRINTSiPR01785. PARAOXONASE.
PR01786. PARAOXONASE1.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Reconsideration of the catalytic center and mechanism of mammalian paraoxonase/arylesterase."
    Sorenson R.C., Primo-Parmo S.L., Kuo C.-L., Adkins S., Lockridge O., La Du B.N.
    Proc. Natl. Acad. Sci. U.S.A. 92:7187-7191(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "Genetic-dietary regulation of serum paraoxonase expression and its role in atherogenesis in a mouse model."
    Shih D.M., Gu L., Hama S., Xia Y.R., Navab M., Fogelman A.M., Lusis A.J.
    J. Clin. Invest. 97:1630-1639(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  3. "Paraoxonase (PON1) gene in mice: sequencing, chromosomal localization and developmental expression."
    Li W.F., Matthews C., Disteche C.M., Costa L.G., Furlong C.E.
    Pharmacogenetics 7:137-144(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  5. "Quorum quenching enzyme activity is widely conserved in the sera of mammalian species."
    Yang F., Wang L.H., Wang J., Dong Y.H., Hu J.Y., Zhang L.H.
    FEBS Lett. 579:3713-3717(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-253.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiPON1_MOUSE
AccessioniPrimary (citable) accession number: P52430
Secondary accession number(s): Q91X30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: June 24, 2015
This is version 125 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces (By similarity).By similarity

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.