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P52430

- PON1_MOUSE

UniProt

P52430 - PON1_MOUSE

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Protein
Serum paraoxonase/arylesterase 1
Gene
Pon1, Pon
Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5 - Experimental evidence at protein leveli

Functioni

Hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. Capable of hydrolyzing a broad spectrum of organophosphate substrates and lactones, and a number of aromatic carboxylic acid esters. Mediates an enzymatic protection of low density lipoproteins against oxidative modification By similarity.

Catalytic activityi

A phenyl acetate + H2O = a phenol + acetate.1 Publication
An aryl dialkyl phosphate + H2O = dialkyl phosphate + an aryl alcohol.1 Publication
An N-acyl-L-homoserine lactone + H2O = an N-acyl-L-homoserine.1 Publication

Cofactori

Binds 2 calcium ions per subunit By similarity.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi53 – 531Calcium 1; catalytic By similarity
Metal bindingi54 – 541Calcium 2 By similarity
Active sitei115 – 1151Proton acceptor By similarity
Metal bindingi117 – 1171Calcium 2; via carbonyl oxygen By similarity
Metal bindingi168 – 1681Calcium 1; catalytic By similarity
Metal bindingi169 – 1691Calcium 2 By similarity
Metal bindingi224 – 2241Calcium 1; catalytic By similarity
Metal bindingi269 – 2691Calcium 1; catalytic By similarity
Metal bindingi270 – 2701Calcium 1; catalytic By similarity

GO - Molecular functioni

  1. aryldialkylphosphatase activity Source: UniProtKB
  2. arylesterase activity Source: UniProtKB
  3. calcium ion binding Source: UniProtKB
  4. high-density lipoprotein particle binding Source: MGI
  5. phospholipid binding Source: Ensembl
Complete GO annotation...

GO - Biological processi

  1. aromatic compound catabolic process Source: Ensembl
  2. blood circulation Source: MGI
  3. carboxylic acid catabolic process Source: Ensembl
  4. cholesterol metabolic process Source: MGI
  5. dephosphorylation Source: GOC
  6. organophosphate catabolic process Source: Ensembl
  7. phosphatidylcholine metabolic process Source: Ensembl
  8. positive regulation of binding Source: Ensembl
  9. positive regulation of cholesterol efflux Source: Ensembl
  10. positive regulation of transporter activity Source: Ensembl
  11. response to toxic substance Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Calcium, Metal-binding

Names & Taxonomyi

Protein namesi
Recommended name:
Serum paraoxonase/arylesterase 1 (EC:3.1.1.2, EC:3.1.1.81, EC:3.1.8.1)
Short name:
PON 1
Alternative name(s):
Aromatic esterase 1
Short name:
A-esterase 1
Serum aryldialkylphosphatase 1
Gene namesi
Name:Pon1
Synonyms:Pon
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Chromosome 6

Organism-specific databases

MGIiMGI:103295. Pon1.

Subcellular locationi

GO - Cellular componenti

  1. intracellular membrane-bounded organelle Source: Ensembl
  2. spherical high-density lipoprotein particle Source: Ensembl
Complete GO annotation...

Keywords - Cellular componenti

HDL, Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Initiator methioninei1 – 11Removed By similarity
Chaini2 – 355354Serum paraoxonase/arylesterase 1
PRO_0000223282Add
BLAST
Signal peptidei2 – ?Not cleaved

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi42 ↔ 353 By similarity
Glycosylationi253 – 2531N-linked (GlcNAc...)1 Publication
Glycosylationi270 – 2701N-linked (GlcNAc...) Reviewed prediction
Glycosylationi324 – 3241N-linked (GlcNAc...) Reviewed prediction

Post-translational modificationi

The signal sequence is not cleaved By similarity.

Keywords - PTMi

Disulfide bond, Glycoprotein

Proteomic databases

MaxQBiP52430.
PaxDbiP52430.
PRIDEiP52430.

PTM databases

PhosphoSiteiP52430.

Expressioni

Tissue specificityi

Plasma, liver, kidney, heart, brain, small intestine and lung. In the plasma, associated with HDL.

Gene expression databases

ArrayExpressiP52430.
BgeeiP52430.
CleanExiMM_PON1.
GenevestigatoriP52430.

Interactioni

Subunit structurei

Homodimer. Interacts with CLU By similarity.

Protein-protein interaction databases

IntActiP52430. 1 interaction.
MINTiMINT-4121591.
STRINGi10090.ENSMUSP00000002663.

Structurei

3D structure databases

ProteinModelPortaliP52430.
SMRiP52430. Positions 20-355.

Family & Domainsi

Sequence similaritiesi

Belongs to the paraoxonase family.

Keywords - Domaini

Signal

Phylogenomic databases

eggNOGiNOG68009.
GeneTreeiENSGT00390000008932.
HOGENOMiHOG000252960.
HOVERGENiHBG003604.
InParanoidiQ91X30.
KOiK01045.
OMAiNAFREVT.
OrthoDBiEOG7GN2N3.
PhylomeDBiP52430.
TreeFamiTF322436.

Family and domain databases

Gene3Di2.120.10.30. 1 hit.
InterProiIPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008363. Paraoxonase1.
[Graphical view]
PfamiPF01731. Arylesterase. 1 hit.
[Graphical view]
PRINTSiPR01785. PARAOXONASE.
PR01786. PARAOXONASE1.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52430-1 [UniParc]FASTAAdd to Basket

« Hide

MAKLLALTLV GLVLALYKNH RSSYQTRLNA FREVTPVELP NCNLVKGIET    50
GAEDLEILPN GLTFFSTGLK YPGIKSFDPS KPGKILLMDL NKKEPAVSEL 100
EIIGNTLDIS SFNPHGISTF TDEDNTVYLL VVNHPDSSST VEVFKFQEEE 150
RSLLHLKTIT HELLPSINDI AAIGPESFYA TNDHYFADPY LRSWEMYLGL 200
SWSNVVYYSP DKVQVVAEGF DFANGIGISL DGKYVYIAEL LAHKIHVYEK 250
HANWTLTPLK VLNFDTLVDN ISVDPVTGDL WVGCHPNGMR IFFYDAENPP 300
GSEVLRIQNI LSEDPKITVV YAENGTVLQG TTVASVYKGK LLIGTVFHKA 350
LYCDL 355
Length:355
Mass (Da):39,565
Last modified:January 23, 2007 - v2
Checksum:iFFA0A71445BB80B4
GO

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti61 – 611G → E in AAH12706. 1 Publication
Sequence conflicti186 – 1861F → L in AAC52496. 1 Publication

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40488 Genomic DNA. Translation: AAA99445.1.
U32684 mRNA. Translation: AAC52496.1.
U72636 mRNA. Translation: AAB17394.1.
BC012706 mRNA. Translation: AAH12706.1.
CCDSiCCDS19898.1.
PIRiT10082.
RefSeqiNP_035264.2. NM_011134.3.
UniGeneiMm.237657.

Genome annotation databases

EnsembliENSMUST00000002663; ENSMUSP00000002663; ENSMUSG00000002588.
GeneIDi18979.
KEGGimmu:18979.
UCSCiuc009awd.2. mouse.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L40488 Genomic DNA. Translation: AAA99445.1 .
U32684 mRNA. Translation: AAC52496.1 .
U72636 mRNA. Translation: AAB17394.1 .
BC012706 mRNA. Translation: AAH12706.1 .
CCDSi CCDS19898.1.
PIRi T10082.
RefSeqi NP_035264.2. NM_011134.3.
UniGenei Mm.237657.

3D structure databases

ProteinModelPortali P52430.
SMRi P52430. Positions 20-355.
ModBasei Search...

Protein-protein interaction databases

IntActi P52430. 1 interaction.
MINTi MINT-4121591.
STRINGi 10090.ENSMUSP00000002663.

PTM databases

PhosphoSitei P52430.

Proteomic databases

MaxQBi P52430.
PaxDbi P52430.
PRIDEi P52430.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

Ensembli ENSMUST00000002663 ; ENSMUSP00000002663 ; ENSMUSG00000002588 .
GeneIDi 18979.
KEGGi mmu:18979.
UCSCi uc009awd.2. mouse.

Organism-specific databases

CTDi 5444.
MGIi MGI:103295. Pon1.

Phylogenomic databases

eggNOGi NOG68009.
GeneTreei ENSGT00390000008932.
HOGENOMi HOG000252960.
HOVERGENi HBG003604.
InParanoidi Q91X30.
KOi K01045.
OMAi NAFREVT.
OrthoDBi EOG7GN2N3.
PhylomeDBi P52430.
TreeFami TF322436.

Miscellaneous databases

NextBioi 295346.
PROi P52430.
SOURCEi Search...

Gene expression databases

ArrayExpressi P52430.
Bgeei P52430.
CleanExi MM_PON1.
Genevestigatori P52430.

Family and domain databases

Gene3Di 2.120.10.30. 1 hit.
InterProi IPR011042. 6-blade_b-propeller_TolB-like.
IPR002640. Arylesterase.
IPR008363. Paraoxonase1.
[Graphical view ]
Pfami PF01731. Arylesterase. 1 hit.
[Graphical view ]
PRINTSi PR01785. PARAOXONASE.
PR01786. PARAOXONASE1.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Reconsideration of the catalytic center and mechanism of mammalian paraoxonase/arylesterase."
    Sorenson R.C., Primo-Parmo S.L., Kuo C.-L., Adkins S., Lockridge O., La Du B.N.
    Proc. Natl. Acad. Sci. U.S.A. 92:7187-7191(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    Strain: BALB/c.
    Tissue: Liver.
  2. "Genetic-dietary regulation of serum paraoxonase expression and its role in atherogenesis in a mouse model."
    Shih D.M., Gu L., Hama S., Xia Y.R., Navab M., Fogelman A.M., Lusis A.J.
    J. Clin. Invest. 97:1630-1639(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: C57BL/6 X CBA.
    Tissue: Liver.
  3. "Paraoxonase (PON1) gene in mice: sequencing, chromosomal localization and developmental expression."
    Li W.F., Matthews C., Disteche C.M., Costa L.G., Furlong C.E.
    Pharmacogenetics 7:137-144(1997) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: BALB/c.
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: FVB/N.
    Tissue: Liver.
  5. "Quorum quenching enzyme activity is widely conserved in the sera of mammalian species."
    Yang F., Wang L.H., Wang J., Dong Y.H., Hu J.Y., Zhang L.H.
    FEBS Lett. 579:3713-3717(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY.
  6. "Enhanced analysis of the mouse plasma proteome using cysteine-containing tryptic glycopeptides."
    Bernhard O.K., Kapp E.A., Simpson R.J.
    J. Proteome Res. 6:987-995(2007) [PubMed] [Europe PMC] [Abstract]
    Cited for: GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-253.
    Strain: C57BL/6.
    Tissue: Plasma.

Entry informationi

Entry nameiPON1_MOUSE
AccessioniPrimary (citable) accession number: P52430
Secondary accession number(s): Q91X30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 9, 2014
This is version 116 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Miscellaneous

The preferential association of PON1 with HDL is mediated in part by its signal peptide, by binding phospholipids directly, rather than binding apo AI. The retained signal peptide may allow transfer of the protein between phospholipid surfaces By similarity.

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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