ID   DGKE_HUMAN              Reviewed;         567 AA.
AC   P52429; Q9UKQ3;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   07-JUL-2009, entry version 76.
DE   RecName: Full=Diacylglycerol kinase epsilon;
DE            Short=DAG kinase epsilon;
DE            EC=2.7.1.107;
DE   AltName: Full=Diglyceride kinase epsilon;
DE            Short=DGK-epsilon;
GN   Name=DGKE; Synonyms=DAGK5;
OS   Homo sapiens (Human).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini;
OC   Catarrhini; Hominidae; Homo.
OX   NCBI_TaxID=9606;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA].
RC   TISSUE=Umbilical vein endothelial cell;
RX   MEDLINE=96215320; PubMed=8626589; DOI=10.1074/jbc.271.17.10230;
RA   Tang W., Bunting M., Zimmerman G.A., McIntyre T.M., Prescott S.M.;
RT   "Molecular cloning of a novel human diacylglycerol kinase highly
RT   selective for arachidonate-containing substrates.";
RL   J. Biol. Chem. 271:10237-10241(1996).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
RX   MEDLINE=20035825; PubMed=10571048; DOI=10.1016/S0378-1119(99)00345-5;
RA   Tang W., Bardien S., Bhattacharya S.S., Prescott S.M.;
RT   "Characterization of the human diacylglycerol kinase epsilon gene and
RT   its assessment as a candidate for inherited retinitis pigmentosa.";
RL   Gene 239:185-192(1999).
RN   [3]
RP   VARIANT [LARGE SCALE ANALYSIS] ARG-99.
RX   PubMed=16959974; DOI=10.1126/science.1133427;
RA   Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D.,
RA   Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S.,
RA   Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J.,
RA   Dawson D., Willson J.K.V., Gazdar A.F., Hartigan J., Wu L., Liu C.,
RA   Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N.,
RA   Vogelstein B., Kinzler K.W., Velculescu V.E.;
RT   "The consensus coding sequences of human breast and colorectal
RT   cancers.";
RL   Science 314:268-274(2006).
CC   -!- FUNCTION: Highly selective for arachidonate-containing species of
CC       diacylglycerol (DAG). May terminate signals transmitted through
CC       arachidonoyl-DAG or may contribute to the synthesis of
CC       phospholipids with defined fatty acid composition.
CC   -!- CATALYTIC ACTIVITY: ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-
CC       sn-glycerol 3-phosphate.
CC   -!- INTERACTION:
CC       Q9Y266:NUDC; NbExp=1; IntAct=EBI-1057499, EBI-357298;
CC       Q9BZD4:NUF2; NbExp=1; IntAct=EBI-1057499, EBI-999865;
CC       P22234:PAICS; NbExp=1; IntAct=EBI-1057499, EBI-712261;
CC       P08559:PDHA1; NbExp=1; IntAct=EBI-1057499, EBI-715747;
CC       Q01105:SET; NbExp=1; IntAct=EBI-1057499, EBI-1053182;
CC   -!- SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein
CC       (Potential).
CC   -!- TISSUE SPECIFICITY: Expressed predominantly in testis.
CC   -!- SIMILARITY: Belongs to the eukaryotic diacylglycerol kinase
CC       family.
CC   -!- SIMILARITY: Contains 1 DAGKc domain.
CC   -!- SIMILARITY: Contains 2 phorbol-ester/DAG-type zinc fingers.
CC   -----------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see http://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution-NoDerivs License
CC   -----------------------------------------------------------------------
DR   EMBL; U49379; AAC50497.1; -; mRNA.
DR   EMBL; AF136745; AAD45666.1; -; Genomic_DNA.
DR   IPI; IPI00003293; -.
DR   RefSeq; NP_003638.1; -.
DR   UniGene; Hs.591179; -.
DR   IntAct; P52429; 7.
DR   PRIDE; P52429; -.
DR   Ensembl; ENSG00000153933; Homo sapiens.
DR   GeneID; 8526; -.
DR   KEGG; hsa:8526; -.
DR   UCSC; uc002iur.1; human.
DR   GeneCards; GC17P052266; -.
DR   H-InvDB; HIX0039161; -.
DR   HGNC; HGNC:2852; DGKE.
DR   HPA; HPA017167; -.
DR   MIM; 601440; gene.
DR   PharmGKB; PA27313; -.
DR   HOGENOM; P52429; -.
DR   HOVERGEN; P52429; -.
DR   OMA; P52429; CVVCKQQ.
DR   BRENDA; 2.7.1.107; 247.
DR   NextBio; 31924; -.
DR   ArrayExpress; P52429; -.
DR   Bgee; P52429; -.
DR   CleanEx; HS_DGKE; -.
DR   GermOnline; ENSG00000153933; Homo sapiens.
DR   GO; GO:0016021; C:integral to membrane; IEA:UniProtKB-KW.
DR   GO; GO:0005524; F:ATP binding; TAS:ProtInc.
DR   GO; GO:0019992; F:diacylglycerol binding; IEA:UniProtKB-KW.
DR   GO; GO:0004143; F:diacylglycerol kinase activity; TAS:ProtInc.
DR   GO; GO:0005515; F:protein binding; IPI:IntAct.
DR   GO; GO:0008270; F:zinc ion binding; IEA:UniProtKB-KW.
DR   GO; GO:0007205; P:activation of protein kinase C activity by ...; IEA:InterPro.
DR   GO; GO:0007242; P:intracellular signaling cascade; IEA:InterPro.
DR   GO; GO:0008654; P:phospholipid biosynthetic process; TAS:ProtInc.
DR   InterPro; IPR002219; DAG_PE_bd.
DR   InterPro; IPR000756; Diacylglycerol_kinase_access.
DR   InterPro; IPR001206; Diacylglycerol_kinase_cat.
DR   Pfam; PF00130; C1_1; 2.
DR   Pfam; PF00609; DAGK_acc; 1.
DR   Pfam; PF00781; DAGK_cat; 1.
DR   ProDom; PD002939; DAGKa; 1.
DR   ProDom; PD005043; DAGKc; 1.
DR   SMART; SM00109; C1; 2.
DR   SMART; SM00045; DAGKa; 1.
DR   SMART; SM00046; DAGKc; 1.
DR   PROSITE; PS00479; ZF_DAG_PE_1; 2.
DR   PROSITE; PS50081; ZF_DAG_PE_2; 2.
PE   1: Evidence at protein level;
KW   ATP-binding; Complete proteome; Kinase; Membrane; Metal-binding;
KW   Nucleotide-binding; Phorbol-ester binding; Polymorphism; Repeat;
KW   Transferase; Transmembrane; Zinc; Zinc-finger.
FT   CHAIN         1    567       Diacylglycerol kinase epsilon.
FT                                /FTId=PRO_0000218464.
FT   TRANSMEM     22     42       Potential.
FT   TRANSMEM    436    456       Potential.
FT   DOMAIN      219    350       DAGKc.
FT   ZN_FING      59    108       Phorbol-ester/DAG-type 1.
FT   ZN_FING     124    177       Phorbol-ester/DAG-type 2.
FT   VARIANT      99     99       L -> R (in a breast cancer sample;
FT                                somatic mutation).
FT                                /FTId=VAR_036120.
SQ   SEQUENCE   567 AA;  63927 MW;  BC334AD15FB4D0B4 CRC64;
     MEAERRPAPG SPSEGLFADG HLILWTLCSV LLPVFITFWC SLQRSRRQLH RRDIFRKSKH
     GWRDTDLFSQ PTYCCVCAQH ILQGAFCDCC GLRVDEGCLR KADKRFQCKE IMLKNDTKVL
     DAMPHHWIRG NVPLCSYCMV CKQQCGCQPK LCDYRCIWCQ KTVHDECMKN SLKNEKCDFG
     EFKNLIIPPS YLTSINQMRK DKKTDYEVLA SKLGKQWTPL IILANSRSGT NMGEGLLGEF
     RILLNPVQVF DVTKTPPIKA LQLCTLLPYY SARVLVCGGD GTVGWVLDAV DDMKIKGQEK
     YIPQVAVLPL GTGNDLSNTL GWGTGYAGEI PVAQVLRNVM EADGIKLDRW KVQVTNKGYY
     NLRKPKEFTM NNYFSVGPDA LMALNFHAHR EKAPSLFSSR ILNKAVYLFY GTKDCLVQEC
     KDLNKKVELE LDGERVALPS LEGIIVLNIG YWGGGCRLWE GMGDETYPLA RHDDGLLEVV
     GVYGSFHCAQ IQVKLANPFR IGQAHTVRLI LKCSMMPMQV DGEPWAQGPC TVTITHKTHA
     MMLYFSGEQT DDDISSTSDQ EDIKATE
//