Reviewed, UniProtKB/Swiss-Prot P52429 (DGKE_HUMAN)

Names and origin

Protein names

Recommended name:
    Diacylglycerol kinase epsilon
      Short name=DAG kinase epsilon
    EC=2.7.1.107
Alternative name(s):
    Diglyceride kinase epsilon
      Short name=DGK-epsilon

Gene names

Name:	DGKE
Synonyms:	DAGK5

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed predominantly in testis.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Transmembrane Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signaling cascade Inferred from electronic annotation. Source: InterPro phospholipid biosynthetic process Ref.1 Traceable author statement. Source: ProtInc
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Ref.1 Traceable author statement. Source: ProtInc diacylglycerol kinase activity Ref.1 Traceable author statement. Source: ProtInc protein binding Inferred from physical interaction. Source: IntAct zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Binary interactions

With	Entry	#Exp.	IntAct	Notes
NUDC	Q9Y266	1	EBI-1057499,EBI-357298
NUF2	Q9BZD4	1	EBI-1057499,EBI-999865
PAICS	P22234	1	EBI-1057499,EBI-712261
PDHA1	P08559	1	EBI-1057499,EBI-715747
SET	Q01105	1	EBI-1057499,EBI-1053182

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Diacylglycerol kinase epsilon		PRO_0000218464
Regions
	Transmembrane	22 – 42	21	Potential
	Transmembrane	436 – 456	21	Potential
	Domain	215 – 356	142	DAGKc
	Zinc finger	59 – 108	50	Phorbol-ester/DAG-type 1
	Zinc finger	124 – 177	54	Phorbol-ester/DAG-type 2
Natural variations
	Natural variant	99	1	L → R in a breast cancer sample; somatic mutation. Ref.3		VAR_036120

Sequences

	Sequence		Length	Mass (Da)
	P52429-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BC334AD15FB4D0B4	FASTA	567	63,927
	10 20 30 40 50 60 MEAERRPAPG SPSEGLFADG HLILWTLCSV LLPVFITFWC SLQRSRRQLH RRDIFRKSKH 70 80 90 100 110 120 GWRDTDLFSQ PTYCCVCAQH ILQGAFCDCC GLRVDEGCLR KADKRFQCKE IMLKNDTKVL 130 140 150 160 170 180 DAMPHHWIRG NVPLCSYCMV CKQQCGCQPK LCDYRCIWCQ KTVHDECMKN SLKNEKCDFG 190 200 210 220 230 240 EFKNLIIPPS YLTSINQMRK DKKTDYEVLA SKLGKQWTPL IILANSRSGT NMGEGLLGEF 250 260 270 280 290 300 RILLNPVQVF DVTKTPPIKA LQLCTLLPYY SARVLVCGGD GTVGWVLDAV DDMKIKGQEK 310 320 330 340 350 360 YIPQVAVLPL GTGNDLSNTL GWGTGYAGEI PVAQVLRNVM EADGIKLDRW KVQVTNKGYY 370 380 390 400 410 420 NLRKPKEFTM NNYFSVGPDA LMALNFHAHR EKAPSLFSSR ILNKAVYLFY GTKDCLVQEC 430 440 450 460 470 480 KDLNKKVELE LDGERVALPS LEGIIVLNIG YWGGGCRLWE GMGDETYPLA RHDDGLLEVV 490 500 510 520 530 540 GVYGSFHCAQ IQVKLANPFR IGQAHTVRLI LKCSMMPMQV DGEPWAQGPC TVTITHKTHA 550 560 MMLYFSGEQT DDDISSTSDQ EDIKATE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates." Tang W., Bunting M., Zimmerman G.A., McIntyre T.M., Prescott S.M. J. Biol. Chem. 271:10237-10241(1996) [PubMed: 8626589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Umbilical vein endothelial cell.
[2]	"Characterization of the human diacylglycerol kinase epsilon gene and its assessment as a candidate for inherited retinitis pigmentosa." Tang W., Bardien S., Bhattacharya S.S., Prescott S.M. Gene 239:185-192(1999) [PubMed: 10571048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
[3]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-99.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49379 mRNA. Translation: AAC50497.1. AF136745 Genomic DNA. Translation: AAD45666.1.
IPI	IPI00003293.
RefSeq	NP_003638.1.
UniGene	Hs.239514
3D structure databases
SMR	P52429. Positions 108-173.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52429. 7 interactions.
STRING	P52429.
Proteomic databases
PRIDE	P52429.
Genome annotation databases
Ensembl	ENST00000284061; ENSP00000284061; ENSG00000153933; Homo sapiens. [Genome view]
GeneID	8526.
KEGG	hsa:8526.
UCSC	uc002iur.1. human.
Organism-specific databases
CTD	8526.
GeneCards	GC17P052266.
H-InvDB	HIX0039161.
HGNC	HGNC:2852. DGKE.
HPA	HPA017167.
MIM	601440. gene.
PharmGKB	PA27313.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG388006.
HOVERGEN	P52429.
InParanoid	P52429.
OMA	CVVCKQQ.
OrthoDB	EOG9H74XK.
PhylomeDB	P52429.
Enzyme and pathway databases
BRENDA	2.7.1.107. 247.
Gene expression databases
ArrayExpress	P52429.
Bgee	P52429.
CleanEx	HS_DGKE.
Genevestigator	P52429.
GermOnline	ENSG00000153933. Homo sapiens.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR002219. Prot_Kinase_C-like_PE/DAG_bd. [Graphical view]
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
NextBio	31924.
SOURCE	Search...

Entry information

Entry name

DGKE_HUMAN

Accession

Primary (citable) accession number: P52429
Secondary accession number(s): Q9UKQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 19, 2010
This is version 83 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.