P52429 (DGKE_HUMAN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Diacylglycerol kinase epsilon
Short name=DAG kinase epsilon
EC=2.7.1.107

Alternative name(s):
Diglyceride kinase epsilon
Short name=DGK-epsilon

Gene names

Name:	DGKE
Synonyms:	DAGK5

Organism

Homo sapiens (Human)

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	567 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Highly selective for arachidonate-containing species of diacylglycerol (DAG). May terminate signals transmitted through arachidonoyl-DAG or may contribute to the synthesis of phospholipids with defined fatty acid composition.
Catalytic activity	ATP + 1,2-diacylglycerol = ADP + 1,2-diacyl-sn-glycerol 3-phosphate.
Subcellular location	Membrane; Multi-pass membrane protein Potential.
Tissue specificity	Expressed predominantly in testis.
Sequence similarities	Belongs to the eukaryotic diacylglycerol kinase family. Contains 1 DAGKc domain. Contains 2 phorbol-ester/DAG-type zinc fingers.

Ontologies

Keywords
Cellular component	Membrane
Coding sequence diversity	Polymorphism
Domain	Repeat Transmembrane Transmembrane helix Zinc-finger
Ligand	ATP-binding Metal-binding Nucleotide-binding Zinc
Molecular function	Kinase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	activation of protein kinase C activity by G-protein coupled receptor protein signaling pathway Inferred from electronic annotation. Source: InterPro intracellular signal transduction Inferred from electronic annotation. Source: InterPro phospholipid biosynthetic process Traceable author statement. Source: ProtInc platelet activation Traceable author statement. Source: Reactome
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW plasma membrane Traceable author statement. Source: Reactome
Molecular function	ATP binding Traceable author statement. Source: ProtInc diacylglycerol kinase activity Traceable author statement. Source: ProtInc metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 567	567	Diacylglycerol kinase epsilon		PRO_0000218464
Regions
	Transmembrane	22 – 42	21	Helical; Potential
	Transmembrane	436 – 456	21	Helical; Potential
	Domain	215 – 356	142	DAGKc
	Zinc finger	59 – 108	50	Phorbol-ester/DAG-type 1
	Zinc finger	124 – 177	54	Phorbol-ester/DAG-type 2
Natural variations
	Natural variant	99	1	L → R in a breast cancer sample; somatic mutation. Ref.3		VAR_036120

Sequences

	Sequence		Length	Mass (Da)
	P52429 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: BC334AD15FB4D0B4	FASTA	567	63,927
	10 20 30 40 50 60 MEAERRPAPG SPSEGLFADG HLILWTLCSV LLPVFITFWC SLQRSRRQLH RRDIFRKSKH 70 80 90 100 110 120 GWRDTDLFSQ PTYCCVCAQH ILQGAFCDCC GLRVDEGCLR KADKRFQCKE IMLKNDTKVL 130 140 150 160 170 180 DAMPHHWIRG NVPLCSYCMV CKQQCGCQPK LCDYRCIWCQ KTVHDECMKN SLKNEKCDFG 190 200 210 220 230 240 EFKNLIIPPS YLTSINQMRK DKKTDYEVLA SKLGKQWTPL IILANSRSGT NMGEGLLGEF 250 260 270 280 290 300 RILLNPVQVF DVTKTPPIKA LQLCTLLPYY SARVLVCGGD GTVGWVLDAV DDMKIKGQEK 310 320 330 340 350 360 YIPQVAVLPL GTGNDLSNTL GWGTGYAGEI PVAQVLRNVM EADGIKLDRW KVQVTNKGYY 370 380 390 400 410 420 NLRKPKEFTM NNYFSVGPDA LMALNFHAHR EKAPSLFSSR ILNKAVYLFY GTKDCLVQEC 430 440 450 460 470 480 KDLNKKVELE LDGERVALPS LEGIIVLNIG YWGGGCRLWE GMGDETYPLA RHDDGLLEVV 490 500 510 520 530 540 GVYGSFHCAQ IQVKLANPFR IGQAHTVRLI LKCSMMPMQV DGEPWAQGPC TVTITHKTHA 550 560 MMLYFSGEQT DDDISSTSDQ EDIKATE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of a novel human diacylglycerol kinase highly selective for arachidonate-containing substrates." Tang W., Bunting M., Zimmerman G.A., McIntyre T.M., Prescott S.M. J. Biol. Chem. 271:10237-10241(1996) [PubMed: 8626589] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Tissue: Umbilical vein endothelial cell.
[2]	"Characterization of the human diacylglycerol kinase epsilon gene and its assessment as a candidate for inherited retinitis pigmentosa." Tang W., Bardien S., Bhattacharya S.S., Prescott S.M. Gene 239:185-192(1999) [PubMed: 10571048] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA] OF 1-154.
[3]	"The consensus coding sequences of human breast and colorectal cancers." Sjoeblom T., Jones S., Wood L.D., Parsons D.W., Lin J., Barber T.D., Mandelker D., Leary R.J., Ptak J., Silliman N., Szabo S., Buckhaults P., Farrell C., Meeh P., Markowitz S.D., Willis J., Dawson D., Willson J.K.V. , Gazdar A.F., Hartigan J., Wu L., Liu C., Parmigiani G., Park B.H., Bachman K.E., Papadopoulos N., Vogelstein B., Kinzler K.W., Velculescu V.E. Science 314:268-274(2006) [PubMed: 16959974] [Abstract] Cited for: VARIANT [LARGE SCALE ANALYSIS] ARG-99.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U49379 mRNA. Translation: AAC50497.1. AF136745 Genomic DNA. Translation: AAD45666.1.
IPI	IPI00003293.
RefSeq	NP_003638.1. NM_003647.2.
UniGene	Hs.239514.
3D structure databases
ProteinModelPortal	P52429.
SMR	P52429. Positions 57-182.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52429. 3 interactions.
STRING	P52429.
PTM databases
PhosphoSite	P52429.
Polymorphism databases
DMDM	1708625.
Proteomic databases
PRIDE	P52429.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENST00000284061; ENSP00000284061; ENSG00000153933.
GeneID	8526.
KEGG	hsa:8526.
UCSC	uc002iur.1. human.
Organism-specific databases
CTD	8526.
GeneCards	GC17P054911.
H-InvDB	HIX0039161.
HGNC	HGNC:2852. DGKE.
HPA	HPA017167.
MIM	601440. gene.
neXtProt	NX_P52429.
GenAtlas	Search...
Phylogenomic databases
HOGENOM	HBG388006.
HOVERGEN	HBG051347.
InParanoid	P52429.
OMA	ASKFGKQ.
OrthoDB	EOG43N7CG.
PhylomeDB	P52429.
Enzyme and pathway databases
Reactome	REACT_111102. Signal Transduction. REACT_604. Hemostasis.
Gene expression databases
ArrayExpress	P52429.
Bgee	P52429.
CleanEx	HS_DGKE.
Genevestigator	P52429.
GermOnline	ENSG00000153933. Homo sapiens.
Family and domain databases
InterPro	IPR000756. Diacylglycerol_kin_accessory. IPR001206. Diacylglycerol_kinase_cat_dom. IPR002219. Prot_Kinase_C-like_PE/DAG-bd. [Graphical view]
KO	K00901.
Pfam	PF00130. C1_1. 2 hits. PF00609. DAGK_acc. 1 hit. PF00781. DAGK_cat. 1 hit. [Graphical view]
SMART	SM00109. C1. 2 hits. SM00045. DAGKa. 1 hit. SM00046. DAGKc. 1 hit. [Graphical view]
PROSITE	PS50146. DAGK. 1 hit. PS00479. ZF_DAG_PE_1. 2 hits. PS50081. ZF_DAG_PE_2. 2 hits. [Graphical view]
ProtoNet	Search...
Other
NextBio	31924.
SOURCE	Search...

Entry information

Entry name

DGKE_HUMAN

Accession

Primary (citable) accession number: P52429
Secondary accession number(s): Q9UKQ3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 101 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.