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P52428 (PSA1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 97. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Proteasome subunit alpha type-1

EC=3.4.25.1
Alternative name(s):
20S proteasome alpha subunit F
20S proteasome subunit alpha-6
Proteasome component C2
Gene names
Name:PAF1
Ordered Locus Names:Os02g0133800, LOC_Os02g04100
ORF Names:P0030G11.18, P0585B01.3
OrganismOryza sativa subsp. japonica (Rice) [Reference proteome]
Taxonomic identifier39947 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaLiliopsidaPoalesPoaceaeBEP cladeEhrhartoideaeOryzeaeOryza

Protein attributes

Sequence length270 AA.
Sequence statusComplete.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.

Catalytic activity

Cleavage of peptide bonds with very broad specificity.

Subunit structure

The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.

Subcellular location

Cytoplasm By similarity. Nucleus By similarity.

Sequence similarities

Belongs to the peptidase T1A family.

Sequence caution

The sequence BAD08003.1 differs from that shown. Reason: Erroneous initiation.

The sequence BAD10085.1 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 270270Proteasome subunit alpha type-1
PRO_0000124074

Sequences

Sequence LengthMass (Da)Tools
P52428 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 37954184C6588900

FASTA27029,630
        10         20         30         40         50         60 
MFRNQYDTDV TTWSPAGRLF QVEYAMEAVK QGSACVGLRS RTHAVLAAAN KAASELSSHQ 

        70         80         90        100        110        120 
RKVFRVADHA GVALAGLTAD GRVLSRFLRS ECINHAFVYD APLPVSRLAL RLADKAQVCT 

       130        140        150        160        170        180 
QRSWKRPYGV GLLVAGLDES GAHLYYNCPS GNYFEYQAFA IGSRSQAAKT FLERRFEGYN 

       190        200        210        220        230        240 
DYTPEQLIKD ALSAIKETLQ GEKLTSSNCT VAIVGRKDDG TVEPFEMIDV KRIQEIIDSM 

       250        260        270 
EAAEEAPAAE AESSSMQEED KGTDAAPMDI 

« Hide

References

« Hide 'large scale' references
[1]"Molecular and biochemical characterization of a proteasome subunit from rice and carrot cells."
Umeda M., Fujii N., Manabe Y., Uchimiya H.
Mol. Gen. Genet. 255:19-27(1997) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]"The map-based sequence of the rice genome."
International rice genome sequencing project (IRGSP)
Nature 436:793-800(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Nipponbare.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
D37886 mRNA. Translation: BAA07128.1.
AP004799 Genomic DNA. Translation: BAD10085.1. Different initiation.
AP004997 Genomic DNA. Translation: BAD08003.1. Different initiation.
PIRT03925.
RefSeqNP_001045808.1. NM_001052343.1.
UniGeneOs.12193.

3D structure databases

ProteinModelPortalP52428.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPST01.976.

Proteomic databases

PaxDbP52428.
PRIDEP52428.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsOS02T0133800-01; OS02T0133800-01; OS02G0133800.
GeneID4328215.
KEGGosa:4328215.

Organism-specific databases

GrameneP52428.

Phylogenomic databases

eggNOGCOG0638.
HOGENOMHOG000091080.
KOK02725.
OMAFMKQQCL.

Family and domain databases

Gene3D3.60.20.10. 1 hit.
InterProIPR029055. Ntn_hydrolases_N.
IPR000426. Proteasome_asu_N.
IPR023332. Proteasome_suA-type.
IPR001353. Proteasome_sua/b.
[Graphical view]
PfamPF00227. Proteasome. 1 hit.
PF10584. Proteasome_A_N. 1 hit.
[Graphical view]
SMARTSM00948. Proteasome_A_N. 1 hit.
[Graphical view]
SUPFAMSSF56235. SSF56235. 1 hit.
PROSITEPS00388. PROTEASOME_ALPHA_1. 1 hit.
PS51475. PROTEASOME_ALPHA_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePSA1_ORYSJ
AccessionPrimary (citable) accession number: P52428
Secondary accession number(s): Q6Z6I2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Oryza sativa (rice)

Index of Oryza sativa entries and their corresponding gene designations

Peptidase families

Classification of peptidase families and list of entries