P52428 (PSA1_ORYSJ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Proteasome subunit alpha type-1
EC=3.4.25.1

Alternative name(s):
20S proteasome alpha subunit F
20S proteasome subunit alpha-6
Proteasome component C2

Gene names

Name:	PAF1
Ordered Locus Names:	Os02g0133800, LOC_Os02g04100
ORF Names:	P0030G11.18, P0585B01.3

Organism

Oryza sativa subsp. japonica (Rice)

Taxonomic identifier

39947 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › Liliopsida › Poales › Poaceae › BEP clade › Ehrhartoideae › Oryzeae › Oryza

Protein attributes

Sequence length	270 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	The proteasome is a multicatalytic proteinase complex which is characterized by its ability to cleave peptides with Arg, Phe, Tyr, Leu, and Glu adjacent to the leaving group at neutral or slightly basic pH. The proteasome has an ATP-dependent proteolytic activity.
Catalytic activity	Cleavage of peptide bonds with very broad specificity.
Subunit structure	The 26S proteasome consists of a 20S proteasome core and two 19S regulatory subunits. The 20S proteasome core is composed of 28 subunits that are arranged in four stacked rings, resulting in a barrel-shaped structure. The two end rings are each formed by seven alpha subunits, and the two central rings are each formed by seven beta subunits. The catalytic chamber with the active sites is on the inside of the barrel By similarity.
Subcellular location	Cytoplasm By similarity. Nucleus By similarity.
Sequence similarities	Belongs to the peptidase T1A family.
Sequence caution	The sequence BAD08003.1 differs from that shown. Reason: Erroneous initiation. The sequence BAD10085.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Cellular component	Cytoplasm Nucleus Proteasome
Molecular function	Hydrolase Protease Threonine protease
Gene Ontology (GO)
Biological process	ubiquitin-dependent protein catabolic process Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell proteasome core complex, alpha-subunit complex Inferred from electronic annotation. Source: InterPro
Molecular function	threonine-type endopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 270	270	Proteasome subunit alpha type-1		PRO_0000124074

Sequences

	Sequence		Length	Mass (Da)
	P52428 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 37954184C6588900	FASTA	270	29,630
	10 20 30 40 50 60 MFRNQYDTDV TTWSPAGRLF QVEYAMEAVK QGSACVGLRS RTHAVLAAAN KAASELSSHQ 70 80 90 100 110 120 RKVFRVADHA GVALAGLTAD GRVLSRFLRS ECINHAFVYD APLPVSRLAL RLADKAQVCT 130 140 150 160 170 180 QRSWKRPYGV GLLVAGLDES GAHLYYNCPS GNYFEYQAFA IGSRSQAAKT FLERRFEGYN 190 200 210 220 230 240 DYTPEQLIKD ALSAIKETLQ GEKLTSSNCT VAIVGRKDDG TVEPFEMIDV KRIQEIIDSM 250 260 270 EAAEEAPAAE AESSSMQEED KGTDAAPMDI

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular and biochemical characterization of a proteasome subunit from rice and carrot cells." Umeda M., Fujii N., Manabe Y., Uchimiya H. Mol. Gen. Genet. 255:19-27(1997) [PubMed: 9230895] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	"The map-based sequence of the rice genome." International rice genome sequencing project (IRGSP) Nature 436:793-800(2005) [PubMed: 16100779] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Nipponbare.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	D37886 mRNA. Translation: BAA07128.1. AP004799 Genomic DNA. Translation: BAD10085.1. Different initiation. AP004997 Genomic DNA. Translation: BAD08003.1. Different initiation.
PIR	T03925.
RefSeq	NP_001045808.1. NM_001052343.1.
UniGene	Os.12193.
3D structure databases
ProteinModelPortal	P52428.
ModBase	Search...
Protein-protein interaction databases
STRING	P52428.
Protein family/group databases
MEROPS	T01.976.
Proteomic databases
PRIDE	P52428.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	LOC_Os02g04100.1; LOC_Os02g04100.1; LOC_Os02g04100.
GeneID	4328215.
KEGG	osa:4328215.
Organism-specific databases
Gramene	P52428.
Phylogenomic databases
OMA	KGPHIYQ.
PhylomeDB	P52428.
ProtClustDB	CLSN2681914.
Family and domain databases
InterPro	IPR000426. Proteasome_asu_N. IPR023332. Proteasome_suA-type. IPR001353. Proteasome_sua/b. [Graphical view]
KO	K02725.
Pfam	PF00227. Proteasome. 1 hit. PF10584. Proteasome_A_N. 1 hit. [Graphical view]
SMART	SM00948. Proteasome_A_N. 1 hit. [Graphical view]
PROSITE	PS00388. PROTEASOME_A_1. 1 hit. PS51475. PROTEASOME_A_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PSA1_ORYSJ

Accession

Primary (citable) accession number: P52428
Secondary accession number(s): Q6Z6I2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 25, 2012
This is version 81 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program