Reviewed, UniProtKB/Swiss-Prot P52425 (GPDA_CUPLA)

Names and origin

Protein names

Recommended name:
    Glycerol-3-phosphate dehydrogenase [NAD+]
    EC=1.1.1.8

Gene names

Name:

GPDH

Organism

Cuphea lanceolata

Taxonomic identifier

3930 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › Myrtales › Lythraceae › Cuphea

Protein attributes

Sequence length	372 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	sn-glycerol 3-phosphate + NAD(+) = glycerone phosphate + NADH.
Sequence similarities	Belongs to the NAD-dependent glycerol-3-phosphate dehydrogenase family.

Ontologies

Keywords
Ligand	NAD
Molecular function	Oxidoreductase
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate catabolic process Inferred from electronic annotation. Source: InterPro oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	glycerol-3-phosphate dehydrogenase complex Inferred from electronic annotation. Source: InterPro
Molecular function	NAD binding Inferred from electronic annotation. Source: InterPro glycerol-3-phosphate dehydrogenase (NAD+) activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 372	372	Glycerol-3-phosphate dehydrogenase [NAD+]		PRO_0000138071
Regions
	Nucleotide binding	29 – 34	6	NAD By similarity
	Region	289 – 290	2	Substrate binding By similarity
Sites
	Active site	225	1	Proton acceptor By similarity
	Binding site	117	1	NAD By similarity
	Binding site	140	1	NAD; via amide nitrogen By similarity
	Binding site	140	1	Substrate By similarity
	Binding site	173	1	NAD; via amide nitrogen By similarity
	Binding site	289	1	NAD By similarity
	Binding site	320	1	NAD By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52425-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 1BF91793CDC23AA9	FASTA	372	40,811
	10 20 30 40 50 60 MAPSELNCTH QNQHSSGYDG PRSRVTVVGS GNWGSVAAKL IATNTLKLPS FHDEVRMWVF 70 80 90 100 110 120 EETLPSGEKL TDVINQTNEN VKYLPGIKLG RNVVADPDLE NAVKDANMLV FVTPHQFMEG 130 140 150 160 170 180 ICKRLEGKIQ EGAQALSLIK GMEVKMEGPC MISSLISDLL GINCCVLMGA NIANEIAVEK 190 200 210 220 230 240 FSEATVGFRE NRDIAEKWVQ LFSTPYFMVS AVEDVEGVEL CGTLKNIVAI AAGFVDGLEM 250 260 270 280 290 300 GNNTKAAIMR IGLREMKAFS KLLFPSVKDT TFFESCGVAD LITTCLGGRN RKVAEAFAKN 310 320 330 340 350 360 GGKRSFDDLE AEMLRGQKLQ GVSTAKEVYE VLGHRGWLEL FPLFSTVHEI STGRLPPSAI 370 VEYSEQKTIF SW

References

[1]	"Cloning of a cDNA coding for a glycerol-3-phosphate dehydrogenase from Cuphea lanceolata." Hausmann L., Schell J., Toepfer R. (In) Kader J.-C., Mazliak P. (eds.); Plant lipid metabolism, pp.534-536, Kluwer Academic Publishers, Dordrecht (1995) Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	X79677 mRNA. Translation: CAA56125.1.
3D structure databases
ModBase	Search...
Family and domain databases
InterPro	IPR013328. DHase_multihelical. IPR016040. NAD(P)-bd. IPR017751. NAD-dep_Gly3P_DH_euk. IPR006168. NAD-dep_Gly3P_DHase. IPR011128. NAD-dep_Gly3P_DHase_N. IPR006109. NAD_Gly3P_DHase_C. [Graphical view]
Gene3D	G3DSA:3.40.50.720. NAD(P)-bd. 1 hit. G3DSA:1.10.1040.10. Opine_DH. 1 hit.
PANTHER	PTHR11728. NAD_Gly3P_DH. 1 hit.
Pfam	PF07479. NAD_Gly3P_dh_C. 1 hit. PF01210. NAD_Gly3P_dh_N. 1 hit. [Graphical view]
PIRSF	PIRSF000114. Glycerol-3-P_dh. 1 hit.
PRINTS	PR00077. GPDHDRGNASE.
ProDom	PD001278. NAD_Gly3P_C. 1 hit. [Graphical view] [Entries sharing at least one domain]
PROSITE	PS00957. NAD_G3PDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GPDA_CUPLA

Accession

Primary (citable) accession number: P52425

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	November 25, 2008
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)