P52424 (PUR5_VIGUN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic/mitochondrial
EC=6.3.3.1

Alternative name(s):
AIR synthase
Short name=AIRS
Phosphoribosyl-aminoimidazole synthetase
VUpur5

Gene names

Name:

PUR5

Organism

Vigna unguiculata (Cowpea)

Taxonomic identifier

3917 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	388 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	ATP + 2-(formamido)-N(1)-(5-phospho-D-ribosyl)acetamidine = ADP + phosphate + 5-amino-1-(5-phospho-D-ribosyl)imidazole.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; 5-amino-1-(5-phospho-D-ribosyl)imidazole from N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide: step 2/2.
Subcellular location	Plastid › chloroplast. Mitochondrion.
Sequence similarities	Belongs to the AIR synthase family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Mitochondrion Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell mitochondrion Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylformylglycinamidine cyclo-ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast and mitochondrion Potential
	Chain	? – 388		Phosphoribosylformylglycinamidine cyclo-ligase, chloroplastic/mitochondrial		PRO_0000029882

Sequences

	Sequence		Length	Mass (Da)
	P52424 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 15D0EF11127C9EE6	FASTA	388	40,427
	10 20 30 40 50 60 MSLSACAELS RCFAAAASAK PNSGKSNSTA ATSLVISSPI GHDGAVSSVS RSRKTSRIVA 70 80 90 100 110 120 EASQGLTYRD AGVDIDAGAE LVRRIAKMAP GIGGFGGLYP LGDSYLVAGT DGVGTKLMLA 130 140 150 160 170 180 FETGIHDTIG IDLVAMSVND IVTSGAKPLF FLDYFATGRL DVDVAEKVVK GIVDGCKQSD 190 200 210 220 230 240 CVLLGGETAE MPGLYKEGEY DLSGCAVGIV KKDSVINGKN IVAGDVIIGL PSSGVHSNGF 250 260 270 280 290 300 SLVRRVLAQS GLSLKDQLPG SNITLAEALM APTVIYVKQV LDLISKGGVK GIAHITGGGF 310 320 330 340 350 360 TDNIPRVFPE GLGALIYDGS WEVPAVFRWL QEAGKIEDSE MRRTFNMGIG MILVVSPEAA 370 380 NRILENKGQA DKFYRIGEII SGNGVTFS

References

[1]	"AIR synthetase in cowpea nodules: a single gene product targeted to two organelles?" Smith P.M.C., Mann A.J., Goggin D.E., Atkins C.A. Plant Mol. Biol. 36:811-820(1998) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Vita 3. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30895 mRNA. Translation: AAC14578.1.
PIR	T10963.
3D structure databases
ProteinModelPortal	P52424.
SMR	P52424. Positions 76-388.
ModBase	Search...
Proteomic databases
ProMEX	P52424.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
BRENDA	6.3.3.1. 6657.
UniPathway	UPA00074; UER00129.
Family and domain databases
Gene3D	3.90.650.10. 1 hit.
InterPro	IPR010918. AIR_synth_C_dom. IPR000728. AIR_synth_N_dom. IPR004733. PurM_cligase. IPR016188. PurM_N-like. [Graphical view]
Pfam	PF00586. AIRS. 1 hit. PF02769. AIRS_C. 1 hit. [Graphical view]
SUPFAM	SSF56042. AIR_synth_C. 1 hit. SSF55326. PurM_N-like. 1 hit.
TIGRFAMs	TIGR00878. purM. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PUR5_VIGUN

Accession

Primary (citable) accession number: P52424

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 29, 2013
This is version 73 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program