P52423 (PUR3_VIGUN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylglycinamide formyltransferase, chloroplastic
EC=2.1.2.2

Alternative name(s):
5'-phosphoribosylglycinamide transformylase
GAR transformylase
Short name=GART

Gene names

Name:

PUR3

Organism

Vigna unguiculata (Cowpea)

Taxonomic identifier

3917 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	312 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	10-formyltetrahydrofolate + N(1)-(5-phospho-D-ribosyl)glycinamide = tetrahydrofolate + N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(2)-formyl-N(1)-(5-phospho-D-ribosyl)glycinamide from N(1)-(5-phospho-D-ribosyl)glycinamide (10-formyl THF route): step 1/1.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the GART family.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Molecular function	Transferase
Gene Ontology (GO)
Biological process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	methyltransferase activity Inferred from electronic annotation. Source: InterPro phosphoribosylglycinamide formyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 73	73	Chloroplast Potential
	Chain	74 – 312	239	Phosphoribosylglycinamide formyltransferase, chloroplastic		PRO_0000029878
Regions
	Region	109 – 111	3	5'-phosphoribosylglycinamide binding By similarity
	Region	187 – 190	4	10-formyltetrahydrofolate binding By similarity
Sites
	Active site	206	1	Proton donor By similarity
	Binding site	162	1	10-formyltetrahydrofolate By similarity
	Binding site	204	1	10-formyltetrahydrofolate By similarity
	Binding site	247	1	10-formyltetrahydrofolate; via amide nitrogen By similarity
	Binding site	276	1	5'-phosphoribosylglycinamide By similarity
	Site	247	1	Raises pKa of active site His By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52423 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 51196B04E3D1385D	FASTA	312	34,583
	10 20 30 40 50 60 MEAQQIISRF CPKSSLAPSI PMVKQPFSLN FPPLHSLSSY PFLQSQNLGF PTGALHAISF 70 80 90 100 110 120 VHKEVCSSSW RIWCSKSSSS TAEPEEDHEV RAQVTVRRKK LAVFVSGGGS NFRSIHEASK 130 140 150 160 170 180 KGSLHGDVTV LVTNKSECGG AQYARNNGIP VILFPKAKDE PKGLSPCDLV DTLRKFEVDF 190 200 210 220 230 240 VLLAGYLKLI PVELIRAFER SIFNIHPSLL PAFGGKGYYG MKVHKAVIAS GARFSGPTIH 250 260 270 280 290 300 FVDEHYDTGR ILAQRVVPVL ANDTAEELAA RVLNEEHQLY VEVVEALCEE RIVWRKDGVP 310 LIQSRENPNE FL

References

[1]	"Vupur3 mRNA from cowpea nodules encoding glycinamide ribonucleotide transformylase." Hall D.J., Bussell J.D., Mann A.J., Goggin D.E., Atkins C.A., Smith P.M.C. Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Vita 3. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30875 mRNA. Translation: AAA75367.2. AF160196 Genomic DNA. Translation: AAD45353.2. AY189137 mRNA. Translation: AAO25114.1. AY189138 mRNA. Translation: AAO25115.1.
PIR	T11574.
3D structure databases
ProteinModelPortal	P52423.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002376. Formyl_transf_N. IPR001555. GART_AS. IPR004607. PurN_trans. [Graphical view]
Gene3D	G3DSA:3.40.50.170. Formyl_transf_N. 1 hit.
Pfam	PF00551. Formyl_trans_N. 1 hit. [Graphical view]
SUPFAM	SSF53328. formyl_transf. 1 hit.
TIGRFAMs	TIGR00639. PurN. 1 hit.
PROSITE	PS00373. GART. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR3_VIGUN

Accession

Primary (citable) accession number: P52423
Secondary accession number(s): Q9XGS3

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 8, 2000
Last modified:	September 21, 2011
This is version 68 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program