P52421 (PUR2_VIGUN) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase
EC=6.3.4.13

Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name=GARS
Phosphoribosylglycinamide synthetase

Gene names

Name:

PUR2

Organism

Vigna unguiculata (Cowpea)

Taxonomic identifier

3917 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Vigna

Protein attributes

Sequence length	311 AA.
Sequence status	Fragment.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylamine-glycine ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 311	›311	Phosphoribosylamine--glycine ligase		PRO_0000151518
Regions
	Domain	‹1 – 191	›191	ATP-grasp
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P52421 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B0CA31A1E2FCB227	FASTA	311	33,070
	10 20 30 40 50 60 DPRVRKQYIQ EQGAPIVIKA DGLAAGKGVT VAMTLEEAYK AVDSMLVQGD FGSAGCRVIV 70 80 90 100 110 120 EEFLEGEEAS FFALVDGENA IPLESAQDHK RVGDGDTGPN TGGMGAYSPA PVLTKELQSI 130 140 150 160 170 180 VMDSIIIPTV KGMSAEGSKF VGVLYAGLMI EKKSGMPKLI EYNVRFGDPE CQVLMVRLES 190 200 210 220 230 240 DLVQVLLAAC RGELNGVSLK WSPGSAMVVV MASKGYPGSY QKGTVIENLE EAEAVAPGIK 250 260 270 280 290 300 IFHAGTAFDS EGRFIATGGR VLGVTAKGND LEEACDRAYL AVENVNWPGG FLPSGYWLES 310 STSETSMLER E

References

[1]	Smith P.M.C., Mann A.J., Hall D.J., Atkins C.A. Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Vita 3. Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U30896 mRNA. Translation: AAA74447.1.
PIR	T11532.
3D structure databases
ProteinModelPortal	P52421.
SMR	P52421. Positions 6-294.
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Enzyme and pathway databases
UniPathway	UPA00074; UER00125.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.90.600.10. 1 hit.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. [Graphical view]
SUPFAM	SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_VIGUN

Accession

Primary (citable) accession number: P52421

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	March 6, 2013
This is version 69 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program