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P52421 (PUR2_VIGUN) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 71. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase

EC=6.3.4.13
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name=GARS
Phosphoribosylglycinamide synthetase
Gene names
Name:PUR2
OrganismVigna unguiculata (Cowpea)
Taxonomic identifier3917 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Protein attributes

Sequence length311 AA.
Sequence statusFragment.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.

Subcellular location

Plastidchloroplast.

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Ontologies

Keywords
   Biological processPurine biosynthesis
   Cellular componentChloroplast
Plastid
   LigandATP-binding
Nucleotide-binding
   Molecular functionLigase
Gene Ontology (GO)
   Biological_process'de novo' IMP biosynthetic process

Inferred from electronic annotation. Source: UniProtKB-UniPathway

purine nucleobase biosynthetic process

Inferred from electronic annotation. Source: InterPro

   Cellular_componentchloroplast

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

metal ion binding

Inferred from electronic annotation. Source: InterPro

phosphoribosylamine-glycine ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain‹1 – 311›311Phosphoribosylamine--glycine ligase
PRO_0000151518

Regions

Domain‹1 – 191›191ATP-grasp

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P52421 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B0CA31A1E2FCB227

FASTA31133,070
        10         20         30         40         50         60 
DPRVRKQYIQ EQGAPIVIKA DGLAAGKGVT VAMTLEEAYK AVDSMLVQGD FGSAGCRVIV 

        70         80         90        100        110        120 
EEFLEGEEAS FFALVDGENA IPLESAQDHK RVGDGDTGPN TGGMGAYSPA PVLTKELQSI 

       130        140        150        160        170        180 
VMDSIIIPTV KGMSAEGSKF VGVLYAGLMI EKKSGMPKLI EYNVRFGDPE CQVLMVRLES 

       190        200        210        220        230        240 
DLVQVLLAAC RGELNGVSLK WSPGSAMVVV MASKGYPGSY QKGTVIENLE EAEAVAPGIK 

       250        260        270        280        290        300 
IFHAGTAFDS EGRFIATGGR VLGVTAKGND LEEACDRAYL AVENVNWPGG FLPSGYWLES 

       310 
STSETSMLER E 

« Hide

References

[1]Smith P.M.C., Mann A.J., Hall D.J., Atkins C.A.
Submitted (JUL-1995) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Vita 3.
Tissue: Root nodule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U30896 mRNA. Translation: AAA74447.1.
PIRT11532.

3D structure databases

ProteinModelPortalP52421.
SMRP52421. Positions 6-294.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00074; UER00125.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.90.600.10. 1 hit.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR2_VIGUN
AccessionPrimary (citable) accession number: P52421
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways