Reviewed, UniProtKB/Swiss-Prot P52420 (PUR2_ARATH)

Names and origin

Protein names

Recommended name:
    Phosphoribosylamine--glycine ligase, chloroplastic
    EC=6.3.4.13
Alternative name(s):
    Glycinamide ribonucleotide synthetase
      Short name=GARS
    Phosphoribosylglycinamide synthetase

Gene names

Name:	PUR2
Ordered Locus Names:	At1g09830
ORF Names:	F21M12.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	purine base biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Ref.1 Inferred from genetic interaction. Source: TAIR
Cellular component	chloroplast Inferred from direct assay. Source: TAIR
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW phosphoribosylamine-glycine ligase activity Ref.1 Inferred from genetic interaction. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 532		Phosphoribosylamine--glycine ligase, chloroplastic		PRO_0000029875
Regions
	Domain	204 – 412	209	ATP-grasp
Experimental info
	Sequence conflict	1 – 91	91	MSSLC…GNGGS → LNPCLRWSESRVLYFDSLPP CRRCVHLIAILHLLLSISSP TTTTQLNPFFSPSDSPPPIL FHLLLLSNSLLPITSLVTLV SLRTESKDAFFCSDAFQKNL SRLFLIG in CAA52778. Ref.1
	Sequence conflict	377 – 380	4	LPKL → FLSF in CAA52778. Ref.1
	Sequence conflict	511 – 532	22	GFFRH…ATKEE → RFL Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52420-1 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 30C81EAB7497152B	FASTA	532	56,473
	10 20 30 40 50 60 MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS TTNHVLSNSR 70 80 90 100 110 120 FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI GGGGREHALC HALKRSPSCD 130 140 150 160 170 180 SVLCAPGNAG ISSSGDATCV PDLDISDSLA VISFCQKWNV GLVVVGPEVP LVAGLANDLV 190 200 210 220 230 240 KAGILTFGPS SQAAALEGSK NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK 250 260 270 280 290 300 ADGLAAGKGV TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN 310 320 330 340 350 360 AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT VKGMAEEGCK 370 380 390 400 410 420 FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE SDLAKVLLAA CKGELSGVSL 430 440 450 460 470 480 DWSKDSAMVV VMASNGYPGS YEKGSIIKNL EEAERVAPGV KVFHAGTGLD SEGNVVATGG 490 500 510 520 530 RVLGVTAKGK DLEEARERAY SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of Arabidopsis thaliana cDNAs encoding three purine biosynthetic enzymes." Schnorr K.M., Nygaard P., Laloue M. Plant J. 6:113-121(1994) [PubMed: 7920700] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed: 11130712] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed: 14593172] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74766 mRNA. Translation: CAA52778.2. Different initiation. AC000132 Genomic DNA. Translation: AAB60737.1. AY050820 mRNA. Translation: AAK92755.1. AY091422 mRNA. Translation: AAM14361.1.
IPI	IPI00527695.
PIR	E86232. S37104.
RefSeq	NP_172454.1.
UniGene	At.42208 At.67297 Rra.26439 Rsa.25587
3D structure databases
SMR	P52420. Positions 94-523.
ModBase	Search...
Protein-protein interaction databases
STRING	P52420.
Proteomic databases
PRIDE	P52420.
Genome annotation databases
GeneID	837515.
GenomeReviews	Gene locus AT1G09830 in contig CT485782_GR.
KEGG	ath:AT1G09830.
NMPDR	fig|3702.1.peg.1215.
Organism-specific databases
TAIR	At1g09830.
Phylogenomic databases
eggNOG	KOG0237.
HOGENOM	HBG404060.
InParanoid	P52420.
OMA	NPHAYEL.
PhylomeDB	P52420.
Enzyme and pathway databases
BRENDA	6.3.4.13. 302.
Gene expression databases
ArrayExpress	P52420.
Genevestigator	P52420.
GermOnline	AT1G09830. Arabidopsis thaliana.
Family and domain databases
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR013817. Pre-ATP_grasp. IPR016185. PreATP-grasp-like. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N-dom. IPR011054. Rudment_hybrid_motif. [Graphical view]
Gene3D	G3DSA:3.30.1490.20. ATP_grasp_subdomain_1. 1 hit. G3DSA:3.30.470.20. ATP_grasp_subdomain_2. 1 hit. G3DSA:3.90.600.10. Gars. 1 hit. G3DSA:3.40.50.20. Pre-ATP_grasp. 1 hit.
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_ARATH

Accession

Primary (citable) accession number: P52420
Secondary accession number(s): O04505

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 8, 2000
Last modified:	February 9, 2010
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)