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P52420 (PUR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified May 14, 2014. Version 114. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Phosphoribosylamine--glycine ligase, chloroplastic

EC=6.3.4.13
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name=GARS
Phosphoribosylglycinamide synthetase
Gene names
Name:PUR2
Ordered Locus Names:At1g09830
ORF Names:F21M12.22
OrganismArabidopsis thaliana (Mouse-ear cress) [Reference proteome]
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis

Protein attributes

Sequence length532 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide. HAMAP-Rule MF_00138

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2. HAMAP-Rule MF_00138

Subcellular location

Plastidchloroplast HAMAP-Rule MF_00138.

Sequence similarities

Belongs to the GARS family.

Contains 1 ATP-grasp domain.

Sequence caution

The sequence CAA52778.2 differs from that shown. Reason: Erroneous initiation.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – ?Chloroplast Potential
Chain? – 532Phosphoribosylamine--glycine ligase, chloroplastic HAMAP-Rule MF_00138PRO_0000029875

Regions

Domain204 – 412209ATP-grasp

Experimental info

Sequence conflict1 – 9191MSSLC…GNGGS → LNPCLRWSESRVLYFDSLPP CRRCVHLIAILHLLLSISSP TTTTQLNPFFSPSDSPPPIL FHLLLLSNSLLPITSLVTLV SLRTESKDAFFCSDAFQKNL SRLFLIG in CAA52778. Ref.1
Sequence conflict377 – 3804LPKL → FLSF in CAA52778. Ref.1
Sequence conflict511 – 53222GFFRH…ATKEE → RFL Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52420 [UniParc].

Last modified December 8, 2000. Version 2.
Checksum: 30C81EAB7497152B

FASTA53256,473
        10         20         30         40         50         60 
MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS TTNHVLSNSR 

        70         80         90        100        110        120 
FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI GGGGREHALC HALKRSPSCD 

       130        140        150        160        170        180 
SVLCAPGNAG ISSSGDATCV PDLDISDSLA VISFCQKWNV GLVVVGPEVP LVAGLANDLV 

       190        200        210        220        230        240 
KAGILTFGPS SQAAALEGSK NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK 

       250        260        270        280        290        300 
ADGLAAGKGV TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN 

       310        320        330        340        350        360 
AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT VKGMAEEGCK 

       370        380        390        400        410        420 
FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE SDLAKVLLAA CKGELSGVSL 

       430        440        450        460        470        480 
DWSKDSAMVV VMASNGYPGS YEKGSIIKNL EEAERVAPGV KVFHAGTGLD SEGNVVATGG 

       490        500        510        520        530 
RVLGVTAKGK DLEEARERAY SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE 

« Hide

References

« Hide 'large scale' references
[1]"Molecular characterization of Arabidopsis thaliana cDNAs encoding three purine biosynthetic enzymes."
Schnorr K.M., Nygaard P., Laloue M.
Plant J. 6:113-121(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Columbia.
[2]"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. expand/collapse author list , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"Empirical analysis of transcriptional activity in the Arabidopsis genome."
Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. expand/collapse author list , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
Strain: cv. Columbia.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
X74766 mRNA. Translation: CAA52778.2. Different initiation.
AC000132 Genomic DNA. Translation: AAB60737.1.
CP002684 Genomic DNA. Translation: AEE28500.1.
AY050820 mRNA. Translation: AAK92755.1.
AY091422 mRNA. Translation: AAM14361.1.
PIRE86232.
S37104.
RefSeqNP_172454.1. NM_100857.3.
UniGeneAt.42208.
At.67297.

3D structure databases

ProteinModelPortalP52420.
SMRP52420. Positions 95-522.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

MINTMINT-8067347.

Proteomic databases

PaxDbP52420.
PRIDEP52420.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT1G09830.1; AT1G09830.1; AT1G09830.
GeneID837515.
KEGGath:AT1G09830.

Organism-specific databases

TAIRAT1G09830.

Phylogenomic databases

eggNOGCOG0151.
HOGENOMHOG000033463.
InParanoidP52420.
KOK01945.
OMAYKGFLYA.
PhylomeDBP52420.

Enzyme and pathway databases

BioCycARA:AT1G09830-MONOMER.
UniPathwayUPA00074; UER00125.

Gene expression databases

GenevestigatorP52420.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPMF_00138. GARS.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR00877. purD. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

PROP52420.

Entry information

Entry namePUR2_ARATH
AccessionPrimary (citable) accession number: P52420
Secondary accession number(s): O04505
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: May 14, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names