P52420 (PUR2_ARATH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phosphoribosylamine--glycine ligase, chloroplastic
EC=6.3.4.13

Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name=GARS
Phosphoribosylglycinamide synthetase

Gene names

Name:	PUR2
Ordered Locus Names:	At1g09830
ORF Names:	F21M12.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Reference proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length	532 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Catalytic activity	ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 2/2.
Subcellular location	Plastid › chloroplast.
Sequence similarities	Belongs to the GARS family. Contains 1 ATP-grasp domain.
Sequence caution	The sequence CAA52778.2 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Transit peptide
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro purine nucleotide biosynthetic process Inferred from genetic interaction Ref.1. Source: TAIR
Cellular_component	chloroplast stroma Inferred from direct assay PubMed 20061580. Source: TAIR
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: InterPro phosphoribosylamine-glycine ligase activity Inferred from genetic interaction Ref.1. Source: TAIR
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Chloroplast Potential
	Chain	? – 532		Phosphoribosylamine--glycine ligase, chloroplastic		PRO_0000029875
Regions
	Domain	204 – 412	209	ATP-grasp
Experimental info
	Sequence conflict	1 – 91	91	MSSLC…GNGGS → LNPCLRWSESRVLYFDSLPP CRRCVHLIAILHLLLSISSP TTTTQLNPFFSPSDSPPPIL FHLLLLSNSLLPITSLVTLV SLRTESKDAFFCSDAFQKNL SRLFLIG in CAA52778. Ref.1
	Sequence conflict	377 – 380	4	LPKL → FLSF in CAA52778. Ref.1
	Sequence conflict	511 – 532	22	GFFRH…ATKEE → RFL Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52420 [UniParc]. Last modified December 8, 2000. Version 2. Checksum: 30C81EAB7497152B	FASTA	532	56,473
	10 20 30 40 50 60 MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS TTNHVLSNSR 70 80 90 100 110 120 FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI GGGGREHALC HALKRSPSCD 130 140 150 160 170 180 SVLCAPGNAG ISSSGDATCV PDLDISDSLA VISFCQKWNV GLVVVGPEVP LVAGLANDLV 190 200 210 220 230 240 KAGILTFGPS SQAAALEGSK NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK 250 260 270 280 290 300 ADGLAAGKGV TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN 310 320 330 340 350 360 AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT VKGMAEEGCK 370 380 390 400 410 420 FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE SDLAKVLLAA CKGELSGVSL 430 440 450 460 470 480 DWSKDSAMVV VMASNGYPGS YEKGSIIKNL EEAERVAPGV KVFHAGTGLD SEGNVVATGG 490 500 510 520 530 RVLGVTAKGK DLEEARERAY SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular characterization of Arabidopsis thaliana cDNAs encoding three purine biosynthetic enzymes." Schnorr K.M., Nygaard P., Laloue M. Plant J. 6:113-121(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Columbia.
[2]	"Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana." Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K. , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W. Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	The Arabidopsis Information Resource (TAIR) Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases Cited for: GENOME REANNOTATION. Strain: cv. Columbia.
[4]	"Empirical analysis of transcriptional activity in the Arabidopsis genome." Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G. , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R. Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA]. Strain: cv. Columbia.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	X74766 mRNA. Translation: CAA52778.2. Different initiation. AC000132 Genomic DNA. Translation: AAB60737.1. CP002684 Genomic DNA. Translation: AEE28500.1. AY050820 mRNA. Translation: AAK92755.1. AY091422 mRNA. Translation: AAM14361.1.
IPI	IPI00527695.
PIR	E86232. S37104.
RefSeq	NP_172454.1. NM_100857.3.
UniGene	At.42208. At.67297.
3D structure databases
ProteinModelPortal	P52420.
SMR	P52420. Positions 95-522.
ModBase	Search...
Proteomic databases
PaxDb	P52420.
PRIDE	P52420.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblPlants	AT1G09830.1; AT1G09830.1; AT1G09830.
GeneID	837515.
KEGG	ath:AT1G09830.
Organism-specific databases
TAIR	At1g09830.
Phylogenomic databases
eggNOG	COG0151.
HOGENOM	HOG000033463.
InParanoid	P52420.
KO	K01945.
OMA	MGAYTPL.
PhylomeDB	P52420.
ProtClustDB	PLN02257.
Enzyme and pathway databases
BioCyc	ARA:AT1G09830-MONOMER.
UniPathway	UPA00074; UER00125.
Gene expression databases
Genevestigator	P52420.
GermOnline	AT1G09830. Arabidopsis thaliana.
Family and domain databases
Gene3D	3.30.1490.20. 1 hit. 3.30.470.20. 1 hit. 3.40.50.20. 1 hit. 3.90.600.10. 1 hit.
InterPro	IPR011761. ATP-grasp. IPR013815. ATP_grasp_subdomain_1. IPR013816. ATP_grasp_subdomain_2. IPR016185. PreATP-grasp_dom. IPR020561. PRibGlycinamid_synth_ATP-grasp. IPR000115. PRibGlycinamide_synth. IPR020560. PRibGlycinamide_synth_C-dom. IPR020559. PRibGlycinamide_synth_CS. IPR020562. PRibGlycinamide_synth_N. IPR011054. Rudment_hybrid_motif. [Graphical view]
Pfam	PF01071. GARS_A. 1 hit. PF02843. GARS_C. 1 hit. PF02844. GARS_N. 1 hit. [Graphical view]
SUPFAM	SSF52440. PreATP-grasp-like. 1 hit. SSF51246. Rudmnt_hyb_motif. 1 hit.
TIGRFAMs	TIGR00877. purD. 1 hit.
PROSITE	PS50975. ATP_GRASP. 1 hit. PS00184. GARS. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR2_ARATH

Accession

Primary (citable) accession number: P52420
Secondary accession number(s): O04505

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	December 8, 2000
Last modified:	May 1, 2013
This is version 106 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program