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Protein

Phosphoribosylamine--glycine ligase, chloroplastic

Gene

PUR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: -Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N1-(5-phospho-D-ribosyl)glycinamide.

Pathwayi: IMP biosynthesis via de novo pathway

This protein is involved in step 2 of the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Amidophosphoribosyltransferase 2, chloroplastic (ASE2), Amidophosphoribosyltransferase (AXX17_At4g39730), Amidophosphoribosyltransferase 1, chloroplastic (ASE1), Amidophosphoribosyltransferase 3, chloroplastic (ASE3), Amidophosphoribosyltransferase (AXX17_At4g44210), Amidophosphoribosyltransferase (AXX17_At2g11630)
  2. Phosphoribosylamine--glycine ligase, chloroplastic (PUR2)
This subpathway is part of the pathway IMP biosynthesis via de novo pathway, which is itself part of Purine metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate, the pathway IMP biosynthesis via de novo pathway and in Purine metabolism.

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processPurine biosynthesis
LigandATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G09830-MONOMER
ReactomeiR-ATH-73817 Purine ribonucleoside monophosphate biosynthesis
UniPathwayiUPA00074; UER00125

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase, chloroplastic (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Gene namesi
Name:PUR2
Ordered Locus Names:At1g09830
ORF Names:F21M12.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 1

Organism-specific databases

AraportiAT1G09830
TAIRilocus:2024321 AT1G09830

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 75ChloroplastCuratedAdd BLAST75
ChainiPRO_000002987576 – 532Phosphoribosylamine--glycine ligase, chloroplasticAdd BLAST457

Proteomic databases

PaxDbiP52420
PRIDEiP52420

Expressioni

Gene expression databases

ExpressionAtlasiP52420 baseline and differential
GenevisibleiP52420 AT

Interactioni

Protein-protein interaction databases

BioGridi22756, 1 interactor
STRINGi3702.AT1G09830.1

Structurei

3D structure databases

ProteinModelPortaliP52420
SMRiP52420
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini204 – 412ATP-graspAdd BLAST209

Sequence similaritiesi

Belongs to the GARS family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0237 Eukaryota
COG0151 LUCA
HOGENOMiHOG000033463
InParanoidiP52420
KOiK01945
OMAiHIQNASF
OrthoDBiEOG0936071A
PhylomeDBiP52420

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
3.90.600.10, 1 hit
HAMAPiMF_00138 GARS, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR016185 PreATP-grasp_dom_sf
IPR020561 PRibGlycinamid_synth_ATP-grasp
IPR000115 PRibGlycinamide_synth
IPR020560 PRibGlycinamide_synth_C-dom
IPR037123 PRibGlycinamide_synth_C_sf
IPR020559 PRibGlycinamide_synth_CS
IPR020562 PRibGlycinamide_synth_N
IPR011054 Rudment_hybrid_motif
PfamiView protein in Pfam
PF01071 GARS_A, 1 hit
PF02843 GARS_C, 1 hit
PF02844 GARS_N, 1 hit
SMARTiView protein in SMART
SM01210 GARS_C, 1 hit
SUPFAMiSSF51246 SSF51246, 1 hit
SSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR00877 purD, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00184 GARS, 1 hit

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS
60 70 80 90 100
TTNHVLSNSR FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI
110 120 130 140 150
GGGGREHALC HALKRSPSCD SVLCAPGNAG ISSSGDATCV PDLDISDSLA
160 170 180 190 200
VISFCQKWNV GLVVVGPEVP LVAGLANDLV KAGILTFGPS SQAAALEGSK
210 220 230 240 250
NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK ADGLAAGKGV
260 270 280 290 300
TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN
310 320 330 340 350
AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT
360 370 380 390 400
VKGMAEEGCK FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE
410 420 430 440 450
SDLAKVLLAA CKGELSGVSL DWSKDSAMVV VMASNGYPGS YEKGSIIKNL
460 470 480 490 500
EEAERVAPGV KVFHAGTGLD SEGNVVATGG RVLGVTAKGK DLEEARERAY
510 520 530
SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE
Length:532
Mass (Da):56,473
Last modified:December 8, 2000 - v2
Checksum:i30C81EAB7497152B
GO

Sequence cautioni

The sequence CAA52778 differs from that shown. Reason: Erroneous initiation.Curated

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti1 – 91MSSLC…GNGGS → LNPCLRWSESRVLYFDSLPP CRRCVHLIAILHLLLSISSP TTTTQLNPFFSPSDSPPPIL FHLLLLSNSLLPITSLVTLV SLRTESKDAFFCSDAFQKNL SRLFLIG in CAA52778 (PubMed:7920700).CuratedAdd BLAST91
Sequence conflicti377 – 380LPKL → FLSF in CAA52778 (PubMed:7920700).Curated4
Sequence conflicti511 – 532GFFRH…ATKEE → RFL (PubMed:7920700).CuratedAdd BLAST22

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74766 mRNA Translation: CAA52778.2 Different initiation.
AC000132 Genomic DNA Translation: AAB60737.1
CP002684 Genomic DNA Translation: AEE28500.1
AY050820 mRNA Translation: AAK92755.1
AY091422 mRNA Translation: AAM14361.1
PIRiE86232
S37104
RefSeqiNP_172454.1, NM_100857.4
UniGeneiAt.42208
At.67297

Genome annotation databases

EnsemblPlantsiAT1G09830.1; AT1G09830.1; AT1G09830
GeneIDi837515
GrameneiAT1G09830.1; AT1G09830.1; AT1G09830
KEGGiath:AT1G09830

Similar proteinsi

Entry informationi

Entry nameiPUR2_ARATH
AccessioniPrimary (citable) accession number: P52420
Secondary accession number(s): O04505
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: May 23, 2018
This is version 137 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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