Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P52420

- PUR2_ARATH

UniProt

P52420 - PUR2_ARATH

Protein

Phosphoribosylamine--glycine ligase, chloroplastic

Gene

PUR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at transcript leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 115 (01 Oct 2014)
      Sequence version 2 (08 Dec 2000)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Catalytic activityi

    ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.

    Pathwayi

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. metal ion binding Source: InterPro
    3. phosphoribosylamine-glycine ligase activity Source: TAIR

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. purine nucleobase biosynthetic process Source: InterPro
    3. purine nucleotide biosynthetic process Source: TAIR

    Keywords - Molecular functioni

    Ligase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BioCyciARA:AT1G09830-MONOMER.
    UniPathwayiUPA00074; UER00125.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Phosphoribosylamine--glycine ligase, chloroplastic (EC:6.3.4.13)
    Alternative name(s):
    Glycinamide ribonucleotide synthetase
    Short name:
    GARS
    Phosphoribosylglycinamide synthetase
    Gene namesi
    Name:PUR2
    Ordered Locus Names:At1g09830
    ORF Names:F21M12.22
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 1

    Organism-specific databases

    TAIRiAT1G09830.

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: TAIR
    2. chloroplast stroma Source: TAIR

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini? – 532Phosphoribosylamine--glycine ligase, chloroplasticPRO_0000029875
    Transit peptidei1 – ?ChloroplastSequence Analysis

    Proteomic databases

    PaxDbiP52420.
    PRIDEiP52420.

    Expressioni

    Gene expression databases

    GenevestigatoriP52420.

    Interactioni

    Protein-protein interaction databases

    MINTiMINT-8067347.

    Structurei

    3D structure databases

    ProteinModelPortaliP52420.
    SMRiP52420. Positions 95-522.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini204 – 412209ATP-graspAdd
    BLAST

    Sequence similaritiesi

    Belongs to the GARS family.Curated
    Contains 1 ATP-grasp domain.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0151.
    HOGENOMiHOG000033463.
    InParanoidiP52420.
    KOiK01945.
    OMAiYKGFLYA.
    PhylomeDBiP52420.

    Family and domain databases

    Gene3Di3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPiMF_00138. GARS.
    InterProiIPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view]
    PfamiPF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view]
    SUPFAMiSSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsiTIGR00877. purD. 1 hit.
    PROSITEiPS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52420-1 [UniParc]FASTAAdd to Basket

    « Hide

    MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS    50
    TTNHVLSNSR FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI 100
    GGGGREHALC HALKRSPSCD SVLCAPGNAG ISSSGDATCV PDLDISDSLA 150
    VISFCQKWNV GLVVVGPEVP LVAGLANDLV KAGILTFGPS SQAAALEGSK 200
    NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK ADGLAAGKGV 250
    TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN 300
    AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT 350
    VKGMAEEGCK FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE 400
    SDLAKVLLAA CKGELSGVSL DWSKDSAMVV VMASNGYPGS YEKGSIIKNL 450
    EEAERVAPGV KVFHAGTGLD SEGNVVATGG RVLGVTAKGK DLEEARERAY 500
    SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE 532
    Length:532
    Mass (Da):56,473
    Last modified:December 8, 2000 - v2
    Checksum:i30C81EAB7497152B
    GO

    Sequence cautioni

    The sequence CAA52778.2 differs from that shown. Reason: Erroneous initiation.

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti1 – 9191MSSLC…GNGGS → LNPCLRWSESRVLYFDSLPP CRRCVHLIAILHLLLSISSP TTTTQLNPFFSPSDSPPPIL FHLLLLSNSLLPITSLVTLV SLRTESKDAFFCSDAFQKNL SRLFLIG in CAA52778. (PubMed:7920700)CuratedAdd
    BLAST
    Sequence conflicti377 – 3804LPKL → FLSF in CAA52778. (PubMed:7920700)Curated
    Sequence conflicti511 – 53222GFFRH…ATKEE → RFL(PubMed:7920700)CuratedAdd
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74766 mRNA. Translation: CAA52778.2. Different initiation.
    AC000132 Genomic DNA. Translation: AAB60737.1.
    CP002684 Genomic DNA. Translation: AEE28500.1.
    AY050820 mRNA. Translation: AAK92755.1.
    AY091422 mRNA. Translation: AAM14361.1.
    PIRiE86232.
    S37104.
    RefSeqiNP_172454.1. NM_100857.3.
    UniGeneiAt.42208.
    At.67297.

    Genome annotation databases

    EnsemblPlantsiAT1G09830.1; AT1G09830.1; AT1G09830.
    GeneIDi837515.
    KEGGiath:AT1G09830.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    X74766 mRNA. Translation: CAA52778.2 . Different initiation.
    AC000132 Genomic DNA. Translation: AAB60737.1 .
    CP002684 Genomic DNA. Translation: AEE28500.1 .
    AY050820 mRNA. Translation: AAK92755.1 .
    AY091422 mRNA. Translation: AAM14361.1 .
    PIRi E86232.
    S37104.
    RefSeqi NP_172454.1. NM_100857.3.
    UniGenei At.42208.
    At.67297.

    3D structure databases

    ProteinModelPortali P52420.
    SMRi P52420. Positions 95-522.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    MINTi MINT-8067347.

    Proteomic databases

    PaxDbi P52420.
    PRIDEi P52420.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT1G09830.1 ; AT1G09830.1 ; AT1G09830 .
    GeneIDi 837515.
    KEGGi ath:AT1G09830.

    Organism-specific databases

    TAIRi AT1G09830.

    Phylogenomic databases

    eggNOGi COG0151.
    HOGENOMi HOG000033463.
    InParanoidi P52420.
    KOi K01945.
    OMAi YKGFLYA.
    PhylomeDBi P52420.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00125 .
    BioCyci ARA:AT1G09830-MONOMER.

    Miscellaneous databases

    PROi P52420.

    Gene expression databases

    Genevestigatori P52420.

    Family and domain databases

    Gene3Di 3.30.1490.20. 1 hit.
    3.30.470.20. 1 hit.
    3.40.50.20. 1 hit.
    3.90.600.10. 1 hit.
    HAMAPi MF_00138. GARS.
    InterProi IPR011761. ATP-grasp.
    IPR013815. ATP_grasp_subdomain_1.
    IPR013816. ATP_grasp_subdomain_2.
    IPR016185. PreATP-grasp_dom.
    IPR020561. PRibGlycinamid_synth_ATP-grasp.
    IPR000115. PRibGlycinamide_synth.
    IPR020560. PRibGlycinamide_synth_C-dom.
    IPR020559. PRibGlycinamide_synth_CS.
    IPR020562. PRibGlycinamide_synth_N.
    IPR011054. Rudment_hybrid_motif.
    [Graphical view ]
    Pfami PF01071. GARS_A. 1 hit.
    PF02843. GARS_C. 1 hit.
    PF02844. GARS_N. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51246. SSF51246. 1 hit.
    SSF52440. SSF52440. 1 hit.
    TIGRFAMsi TIGR00877. purD. 1 hit.
    PROSITEi PS50975. ATP_GRASP. 1 hit.
    PS00184. GARS. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular characterization of Arabidopsis thaliana cDNAs encoding three purine biosynthetic enzymes."
      Schnorr K.M., Nygaard P., Laloue M.
      Plant J. 6:113-121(1994) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Columbia.
    2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
      Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
      , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
      Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
      Strain: cv. Columbia.

    Entry informationi

    Entry nameiPUR2_ARATH
    AccessioniPrimary (citable) accession number: P52420
    Secondary accession number(s): O04505
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: December 8, 2000
    Last modified: October 1, 2014
    This is version 115 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3