SubmitCancel

Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.

P52420

- PUR2_ARATH

UniProt

P52420 - PUR2_ARATH

(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

Protein
Phosphoribosylamine--glycine ligase, chloroplastic
Gene
PUR2, At1g09830, F21M12.22
Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.UniRule annotation

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: InterPro
  3. phosphoribosylamine-glycine ligase activity Source: TAIR

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
  3. purine nucleotide biosynthetic process Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G09830-MONOMER.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase, chloroplastic (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Gene namesi
Name:PUR2
Ordered Locus Names:At1g09830
ORF Names:F21M12.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 1

Organism-specific databases

TAIRiAT1G09830.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 532Phosphoribosylamine--glycine ligase, chloroplasticUniRule annotationPRO_0000029875
Transit peptidei1 – ?Chloroplast Reviewed prediction

Proteomic databases

PaxDbiP52420.
PRIDEiP52420.

Expressioni

Gene expression databases

GenevestigatoriP52420.

Interactioni

Protein-protein interaction databases

MINTiMINT-8067347.

Structurei

3D structure databases

ProteinModelPortaliP52420.
SMRiP52420. Positions 95-522.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 412209ATP-grasp
Add
BLAST

Sequence similaritiesi

Belongs to the GARS family.
Contains 1 ATP-grasp domain.

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033463.
InParanoidiP52420.
KOiK01945.
OMAiYKGFLYA.
PhylomeDBiP52420.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52420-1 [UniParc]FASTAAdd to Basket

« Hide

MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS    50
TTNHVLSNSR FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI 100
GGGGREHALC HALKRSPSCD SVLCAPGNAG ISSSGDATCV PDLDISDSLA 150
VISFCQKWNV GLVVVGPEVP LVAGLANDLV KAGILTFGPS SQAAALEGSK 200
NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK ADGLAAGKGV 250
TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN 300
AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT 350
VKGMAEEGCK FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE 400
SDLAKVLLAA CKGELSGVSL DWSKDSAMVV VMASNGYPGS YEKGSIIKNL 450
EEAERVAPGV KVFHAGTGLD SEGNVVATGG RVLGVTAKGK DLEEARERAY 500
SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE 532
Length:532
Mass (Da):56,473
Last modified:December 8, 2000 - v2
Checksum:i30C81EAB7497152B
GO

Sequence cautioni

The sequence CAA52778.2 differs from that shown. Reason: Erroneous initiation.

Sequence conflict

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 9191MSSLC…GNGGS → LNPCLRWSESRVLYFDSLPP CRRCVHLIAILHLLLSISSP TTTTQLNPFFSPSDSPPPIL FHLLLLSNSLLPITSLVTLV SLRTESKDAFFCSDAFQKNL SRLFLIG in CAA52778. 1 Publication
Add
BLAST
Sequence conflicti377 – 3804LPKL → FLSF in CAA52778. 1 Publication
Sequence conflicti511 – 53222GFFRH…ATKEE → RFL1 Publication
Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74766 mRNA. Translation: CAA52778.2. Different initiation.
AC000132 Genomic DNA. Translation: AAB60737.1.
CP002684 Genomic DNA. Translation: AEE28500.1.
AY050820 mRNA. Translation: AAK92755.1.
AY091422 mRNA. Translation: AAM14361.1.
PIRiE86232.
S37104.
RefSeqiNP_172454.1. NM_100857.3.
UniGeneiAt.42208.
At.67297.

Genome annotation databases

EnsemblPlantsiAT1G09830.1; AT1G09830.1; AT1G09830.
GeneIDi837515.
KEGGiath:AT1G09830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
X74766 mRNA. Translation: CAA52778.2 . Different initiation.
AC000132 Genomic DNA. Translation: AAB60737.1 .
CP002684 Genomic DNA. Translation: AEE28500.1 .
AY050820 mRNA. Translation: AAK92755.1 .
AY091422 mRNA. Translation: AAM14361.1 .
PIRi E86232.
S37104.
RefSeqi NP_172454.1. NM_100857.3.
UniGenei At.42208.
At.67297.

3D structure databases

ProteinModelPortali P52420.
SMRi P52420. Positions 95-522.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

MINTi MINT-8067347.

Proteomic databases

PaxDbi P52420.
PRIDEi P52420.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT1G09830.1 ; AT1G09830.1 ; AT1G09830 .
GeneIDi 837515.
KEGGi ath:AT1G09830.

Organism-specific databases

TAIRi AT1G09830.

Phylogenomic databases

eggNOGi COG0151.
HOGENOMi HOG000033463.
InParanoidi P52420.
KOi K01945.
OMAi YKGFLYA.
PhylomeDBi P52420.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00125 .
BioCyci ARA:AT1G09830-MONOMER.

Miscellaneous databases

PROi P52420.

Gene expression databases

Genevestigatori P52420.

Family and domain databases

Gene3Di 3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPi MF_00138. GARS.
InterProi IPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view ]
Pfami PF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view ]
SUPFAMi SSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsi TIGR00877. purD. 1 hit.
PROSITEi PS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of Arabidopsis thaliana cDNAs encoding three purine biosynthetic enzymes."
    Schnorr K.M., Nygaard P., Laloue M.
    Plant J. 6:113-121(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPUR2_ARATH
AccessioniPrimary (citable) accession number: P52420
Secondary accession number(s): O04505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: May 14, 2014
This is version 114 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi