Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Phosphoribosylamine--glycine ligase, chloroplastic

Gene

PUR2

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at transcript leveli

Functioni

Catalytic activityi

ATP + 5-phospho-D-ribosylamine + glycine = ADP + phosphate + N(1)-(5-phospho-D-ribosyl)glycinamide.

Pathwayi

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. metal ion binding Source: InterPro
  3. phosphoribosylamine-glycine ligase activity Source: UniProtKB-EC

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciARA:AT1G09830-MONOMER.
ReactomeiREACT_350544. Purine ribonucleoside monophosphate biosynthesis.
UniPathwayiUPA00074; UER00125.

Names & Taxonomyi

Protein namesi
Recommended name:
Phosphoribosylamine--glycine ligase, chloroplastic (EC:6.3.4.13)
Alternative name(s):
Glycinamide ribonucleotide synthetase
Short name:
GARS
Phosphoribosylglycinamide synthetase
Gene namesi
Name:PUR2
Ordered Locus Names:At1g09830
ORF Names:F21M12.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548 Componenti: Chromosome 1

Organism-specific databases

TAIRiAT1G09830.

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: TAIR
  2. chloroplast stroma Source: TAIR
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini? – 532Phosphoribosylamine--glycine ligase, chloroplasticPRO_0000029875
Transit peptidei1 – ?ChloroplastSequence Analysis

Proteomic databases

PaxDbiP52420.
PRIDEiP52420.

Expressioni

Gene expression databases

GenevestigatoriP52420.

Interactioni

Protein-protein interaction databases

MINTiMINT-8067347.

Structurei

3D structure databases

ProteinModelPortaliP52420.
SMRiP52420. Positions 95-522.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini204 – 412209ATP-graspAdd
BLAST

Sequence similaritiesi

Belongs to the GARS family.Curated
Contains 1 ATP-grasp domain.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033463.
InParanoidiP52420.
KOiK01945.
OMAiGPMTGGM.
PhylomeDBiP52420.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52420-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSSLCASNCY PSSSSINLFS NNNNPTKPFL LSLRFASSNS LPFVAPLKFS
60 70 80 90 100
TTNHVLSNSR FSSNRIQRRL FLLRCVSEES QPSLSIGNGG SEERVNVLVI
110 120 130 140 150
GGGGREHALC HALKRSPSCD SVLCAPGNAG ISSSGDATCV PDLDISDSLA
160 170 180 190 200
VISFCQKWNV GLVVVGPEVP LVAGLANDLV KAGILTFGPS SQAAALEGSK
210 220 230 240 250
NFMKNLCHKY NIPTAKYKTF SDASAAKEYI QEQGAPIVIK ADGLAAGKGV
260 270 280 290 300
TVAMELEEAF EAVDSMLVKG VFGSAGCQVV VEEFLEGEEA SFFALVDGEN
310 320 330 340 350
AIPLESAQDH KRVGDGDTGP NTGGMGAYSP APVLTKELQD FVMESIIHPT
360 370 380 390 400
VKGMAEEGCK FVGVLFAGLM IEKKSGLPKL IEFNVRFGDP ECQVLMMRLE
410 420 430 440 450
SDLAKVLLAA CKGELSGVSL DWSKDSAMVV VMASNGYPGS YEKGSIIKNL
460 470 480 490 500
EEAERVAPGV KVFHAGTGLD SEGNVVATGG RVLGVTAKGK DLEEARERAY
510 520 530
SAVQQINWPG GFFRHDIGWR ALRQKQVATK EE
Length:532
Mass (Da):56,473
Last modified:December 8, 2000 - v2
Checksum:i30C81EAB7497152B
GO

Sequence cautioni

The sequence CAA52778.2 differs from that shown. Reason: Erroneous initiation. Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti1 – 9191MSSLC…GNGGS → LNPCLRWSESRVLYFDSLPP CRRCVHLIAILHLLLSISSP TTTTQLNPFFSPSDSPPPIL FHLLLLSNSLLPITSLVTLV SLRTESKDAFFCSDAFQKNL SRLFLIG in CAA52778 (PubMed:7920700).CuratedAdd
BLAST
Sequence conflicti377 – 3804LPKL → FLSF in CAA52778 (PubMed:7920700).Curated
Sequence conflicti511 – 53222GFFRH…ATKEE → RFL (PubMed:7920700).CuratedAdd
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74766 mRNA. Translation: CAA52778.2. Different initiation.
AC000132 Genomic DNA. Translation: AAB60737.1.
CP002684 Genomic DNA. Translation: AEE28500.1.
AY050820 mRNA. Translation: AAK92755.1.
AY091422 mRNA. Translation: AAM14361.1.
PIRiE86232.
S37104.
RefSeqiNP_172454.1. NM_100857.3.
UniGeneiAt.42208.
At.67297.

Genome annotation databases

EnsemblPlantsiAT1G09830.1; AT1G09830.1; AT1G09830.
GeneIDi837515.
KEGGiath:AT1G09830.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
X74766 mRNA. Translation: CAA52778.2. Different initiation.
AC000132 Genomic DNA. Translation: AAB60737.1.
CP002684 Genomic DNA. Translation: AEE28500.1.
AY050820 mRNA. Translation: AAK92755.1.
AY091422 mRNA. Translation: AAM14361.1.
PIRiE86232.
S37104.
RefSeqiNP_172454.1. NM_100857.3.
UniGeneiAt.42208.
At.67297.

3D structure databases

ProteinModelPortaliP52420.
SMRiP52420. Positions 95-522.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

MINTiMINT-8067347.

Proteomic databases

PaxDbiP52420.
PRIDEiP52420.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT1G09830.1; AT1G09830.1; AT1G09830.
GeneIDi837515.
KEGGiath:AT1G09830.

Organism-specific databases

TAIRiAT1G09830.

Phylogenomic databases

eggNOGiCOG0151.
HOGENOMiHOG000033463.
InParanoidiP52420.
KOiK01945.
OMAiGPMTGGM.
PhylomeDBiP52420.

Enzyme and pathway databases

UniPathwayiUPA00074; UER00125.
BioCyciARA:AT1G09830-MONOMER.
ReactomeiREACT_350544. Purine ribonucleoside monophosphate biosynthesis.

Miscellaneous databases

PROiP52420.

Gene expression databases

GenevestigatoriP52420.

Family and domain databases

Gene3Di3.30.1490.20. 1 hit.
3.30.470.20. 1 hit.
3.40.50.20. 1 hit.
3.90.600.10. 1 hit.
HAMAPiMF_00138. GARS.
InterProiIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR016185. PreATP-grasp_dom.
IPR020561. PRibGlycinamid_synth_ATP-grasp.
IPR000115. PRibGlycinamide_synth.
IPR020560. PRibGlycinamide_synth_C-dom.
IPR020559. PRibGlycinamide_synth_CS.
IPR020562. PRibGlycinamide_synth_N.
IPR011054. Rudment_hybrid_motif.
[Graphical view]
PfamiPF01071. GARS_A. 1 hit.
PF02843. GARS_C. 1 hit.
PF02844. GARS_N. 1 hit.
[Graphical view]
SUPFAMiSSF51246. SSF51246. 1 hit.
SSF52440. SSF52440. 1 hit.
TIGRFAMsiTIGR00877. purD. 1 hit.
PROSITEiPS50975. ATP_GRASP. 1 hit.
PS00184. GARS. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Molecular characterization of Arabidopsis thaliana cDNAs encoding three purine biosynthetic enzymes."
    Schnorr K.M., Nygaard P., Laloue M.
    Plant J. 6:113-121(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Columbia.
  2. "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana."
    Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O., Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E., Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K.
    , Conn L., Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P., Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D., Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J., Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L., Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A., Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A., Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M., Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M., Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P., Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D., Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D., Yu G., Fraser C.M., Venter J.C., Davis R.W.
    Nature 408:816-820(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Strain: cv. Columbia.

Entry informationi

Entry nameiPUR2_ARATH
AccessioniPrimary (citable) accession number: P52420
Secondary accession number(s): O04505
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 8, 2000
Last modified: April 1, 2015
This is version 118 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.