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P52419

- PUR1_VIGAC

UniProt

P52419 - PUR1_VIGAC

Protein

Amidophosphoribosyltransferase, chloroplastic

Gene

PUR1

Organism
Vigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 83 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei19 – 191For GATase activityBy similarity
    Metal bindingi253 – 2531Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi300 – 3001MagnesiumBy similarity
    Metal bindingi362 – 3621MagnesiumBy similarity
    Metal bindingi363 – 3631MagnesiumBy similarity
    Metal bindingi399 – 3991Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi450 – 4501Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi453 – 4531Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase, chloroplastic (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPAT
    Gene namesi
    Name:PUR1
    OrganismiVigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
    Taxonomic identifieri3918 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei‹1 – 18›18ChloroplastSequence AnalysisAdd
    BLAST
    Chaini19 – 485467Amidophosphoribosyltransferase, chloroplasticPRO_0000029290Add
    BLAST

    Structurei

    3D structure databases

    ProteinModelPortaliP52419.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini19 – 237219Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Transit peptide

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Fragment.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52419-1 [UniParc]FASTAAdd to Basket

    « Hide

    KTTNTFASVN DDEKPREECG VVGIYGDPEA SRLCSLALHA LQHRGQEGAG    50
    IVAVHDNLFH QVNGVGLVSD VFNEAKLSEL PGSCAIGHVR YSTAGHSKLV 100
    NVQPFVAGYR FGSVAVAHNG NFVNYRSLRA KLEDNGSIFN TTSDTEVVLH 150
    LIATSKHRPF LLRVVDACEN LKGAYSLVFL TEDKLVAVRD FGFRPLVMGR 200
    RKNGAVVFAS ETCALDLIDA TYEREVNPGE VVVVDHTGIQ SLCLVTHQEP 250
    KQCIFEHIYF ALPNSVVFGR SVYESRRKFG EILATESPVE CDVVIAVPDS 300
    GVVAALGYAA KAGVPFQQGL IRSHHVGRTF IEPSQKIRDF GVKLKLFPVR 350
    GVLEGKRVVV VDDSIVRGTT SSKIVRLIKE AGAKEVHMRI ACPPIVASCY 400
    YGVDTPSKEE LISNRMDVEE IRKFIGSDSL AFLPLDTLKS LLEDDAPNYC 450
    YACFSGKYPV QPENLNPTAS MSLTGTMFQW QFETY 485
    Length:485
    Mass (Da):53,205
    Last modified:October 1, 1996 - v1
    Checksum:iEE9CD20AE5BEDD82
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Non-terminal residuei1 – 11

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23834 mRNA. Translation: AAA73944.1.
    PIRiT10792.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23834 mRNA. Translation: AAA73944.1 .
    PIRi T10792.

    3D structure databases

    ProteinModelPortali P52419.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
      Kim J.H., Delauney A.J., Verma D.P.S.
      Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Tissue: Root nodule.

    Entry informationi

    Entry nameiPUR1_VIGAC
    AccessioniPrimary (citable) accession number: P52419
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 83 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3