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P52419 (PUR1_VIGAC) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 82. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase, chloroplastic

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPAT
Gene names
Name:PUR1
OrganismVigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Taxonomic identifier3918 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Protein attributes

Sequence length485 AA.
Sequence statusFragment.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Subcellular location

Plastidchloroplast.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide‹1 – 18›18Chloroplast Potential
Chain19 – 485467Amidophosphoribosyltransferase, chloroplastic
PRO_0000029290

Regions

Domain19 – 237219Glutamine amidotransferase type-2

Sites

Active site191For GATase activity By similarity
Metal binding2531Iron-sulfur (4Fe-4S) By similarity
Metal binding3001Magnesium By similarity
Metal binding3621Magnesium By similarity
Metal binding3631Magnesium By similarity
Metal binding3991Iron-sulfur (4Fe-4S) By similarity
Metal binding4501Iron-sulfur (4Fe-4S) By similarity
Metal binding4531Iron-sulfur (4Fe-4S) By similarity

Experimental info

Non-terminal residue11

Sequences

Sequence LengthMass (Da)Tools
P52419 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: EE9CD20AE5BEDD82

FASTA48553,205
        10         20         30         40         50         60 
KTTNTFASVN DDEKPREECG VVGIYGDPEA SRLCSLALHA LQHRGQEGAG IVAVHDNLFH 

        70         80         90        100        110        120 
QVNGVGLVSD VFNEAKLSEL PGSCAIGHVR YSTAGHSKLV NVQPFVAGYR FGSVAVAHNG 

       130        140        150        160        170        180 
NFVNYRSLRA KLEDNGSIFN TTSDTEVVLH LIATSKHRPF LLRVVDACEN LKGAYSLVFL 

       190        200        210        220        230        240 
TEDKLVAVRD FGFRPLVMGR RKNGAVVFAS ETCALDLIDA TYEREVNPGE VVVVDHTGIQ 

       250        260        270        280        290        300 
SLCLVTHQEP KQCIFEHIYF ALPNSVVFGR SVYESRRKFG EILATESPVE CDVVIAVPDS 

       310        320        330        340        350        360 
GVVAALGYAA KAGVPFQQGL IRSHHVGRTF IEPSQKIRDF GVKLKLFPVR GVLEGKRVVV 

       370        380        390        400        410        420 
VDDSIVRGTT SSKIVRLIKE AGAKEVHMRI ACPPIVASCY YGVDTPSKEE LISNRMDVEE 

       430        440        450        460        470        480 
IRKFIGSDSL AFLPLDTLKS LLEDDAPNYC YACFSGKYPV QPENLNPTAS MSLTGTMFQW 


QFETY 

« Hide

References

[1]"Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
Kim J.H., Delauney A.J., Verma D.P.S.
Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Tissue: Root nodule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23834 mRNA. Translation: AAA73944.1.
PIRT10792.

3D structure databases

ProteinModelPortalP52419.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.001.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Enzyme and pathway databases

UniPathwayUPA00074; UER00124.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_VIGAC
AccessionPrimary (citable) accession number: P52419
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 82 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways