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P52419

- PUR1_VIGAC

UniProt

P52419 - PUR1_VIGAC

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Protein

Amidophosphoribosyltransferase, chloroplastic

Gene

PUR1

Organism
Vigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit.By similarity
Binds 1 4Fe-4S cluster per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei19 – 191NucleophilePROSITE-ProRule annotation
Metal bindingi253 – 2531Iron-sulfur (4Fe-4S)By similarity
Metal bindingi300 – 3001MagnesiumBy similarity
Metal bindingi362 – 3621MagnesiumBy similarity
Metal bindingi363 – 3631MagnesiumBy similarity
Metal bindingi399 – 3991Iron-sulfur (4Fe-4S)By similarity
Metal bindingi450 – 4501Iron-sulfur (4Fe-4S)By similarity
Metal bindingi453 – 4531Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase, chloroplastic (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPAT
Gene namesi
Name:PUR1
OrganismiVigna aconitifolia (Moth bean) (Phaseolus aconitifolius)
Taxonomic identifieri3918 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeVigna

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei‹1 – 18›18ChloroplastSequence AnalysisAdd
BLAST
Chaini19 – 485467Amidophosphoribosyltransferase, chloroplasticPRO_0000029290Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP52419.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini19 – 237219Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Transit peptide

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52419-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
KTTNTFASVN DDEKPREECG VVGIYGDPEA SRLCSLALHA LQHRGQEGAG
60 70 80 90 100
IVAVHDNLFH QVNGVGLVSD VFNEAKLSEL PGSCAIGHVR YSTAGHSKLV
110 120 130 140 150
NVQPFVAGYR FGSVAVAHNG NFVNYRSLRA KLEDNGSIFN TTSDTEVVLH
160 170 180 190 200
LIATSKHRPF LLRVVDACEN LKGAYSLVFL TEDKLVAVRD FGFRPLVMGR
210 220 230 240 250
RKNGAVVFAS ETCALDLIDA TYEREVNPGE VVVVDHTGIQ SLCLVTHQEP
260 270 280 290 300
KQCIFEHIYF ALPNSVVFGR SVYESRRKFG EILATESPVE CDVVIAVPDS
310 320 330 340 350
GVVAALGYAA KAGVPFQQGL IRSHHVGRTF IEPSQKIRDF GVKLKLFPVR
360 370 380 390 400
GVLEGKRVVV VDDSIVRGTT SSKIVRLIKE AGAKEVHMRI ACPPIVASCY
410 420 430 440 450
YGVDTPSKEE LISNRMDVEE IRKFIGSDSL AFLPLDTLKS LLEDDAPNYC
460 470 480
YACFSGKYPV QPENLNPTAS MSLTGTMFQW QFETY
Length:485
Mass (Da):53,205
Last modified:October 1, 1996 - v1
Checksum:iEE9CD20AE5BEDD82
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23834 mRNA. Translation: AAA73944.1.
PIRiT10792.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23834 mRNA. Translation: AAA73944.1 .
PIRi T10792.

3D structure databases

ProteinModelPortali P52419.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.001.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPi MF_01931. PurF.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
    Kim J.H., Delauney A.J., Verma D.P.S.
    Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Tissue: Root nodule.

Entry informationi

Entry nameiPUR1_VIGAC
AccessioniPrimary (citable) accession number: P52419
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 84 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3