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P52418 (PUR1_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 89. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Amidophosphoribosyltransferase, chloroplastic

Short name=ATase
EC=2.4.2.14
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPAT
Gene names
Name:PUR1
OrganismGlycine max (Soybean) (Glycine hispida) [Reference proteome]
Taxonomic identifier3847 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja

Protein attributes

Sequence length569 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level

General annotation (Comments)

Catalytic activity

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactor

Binds 1 magnesium ion per subunit By similarity.

Binds 1 4Fe-4S cluster per subunit By similarity.

Pathway

Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.

Subcellular location

Plastidchloroplast.

Sequence similarities

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Contains 1 glutamine amidotransferase type-2 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 8080Chloroplast Potential
Chain81 – 569489Amidophosphoribosyltransferase, chloroplastic
PRO_0000029289

Regions

Domain81 – 301221Glutamine amidotransferase type-2

Sites

Active site811For GATase activity By similarity
Metal binding3171Iron-sulfur (4Fe-4S) By similarity
Metal binding3641Magnesium By similarity
Metal binding4261Magnesium By similarity
Metal binding4271Magnesium By similarity
Metal binding4631Iron-sulfur (4Fe-4S) By similarity
Metal binding5141Iron-sulfur (4Fe-4S) By similarity
Metal binding5171Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence LengthMass (Da)Tools
P52418 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 33E36AB22F616491

FASTA56962,205
        10         20         30         40         50         60 
MAAASNLSTL SSSSLSKPSS SFPFRNTPTN NNASFLHNKS LPNQNSLSHK LSSPLPLACN 

        70         80         90        100        110        120 
PKNQNTCVFF DDEDQKPREE CGVVGIYGDP EASRLCSLAL HALQHRGQEG AGIVAVHDNH 

       130        140        150        160        170        180 
LQSVTGVGLV SDVFEQSKLS RLPGTSAIGH VRYSTAGQSM LKNVQPFLAD YRFAAVAVAH 

       190        200        210        220        230        240 
NGNFVNYRSL RARLEHNNGS IFNTTSDTEV VLHLIATSKH RPFLLRIVDA CEHLQGAYSL 

       250        260        270        280        290        300 
VFVTEDKLVA VRDPFGFRPL VMGRRTNGAV VLASETCALD LIEATYEREV YPGEVIVVDH 

       310        320        330        340        350        360 
TGIQSLCLVS HPEPKQCIFE HIYFALPNSV VFGRSVYESR KKFGEILASE SPVECDVVIA 

       370        380        390        400        410        420 
VPDSGVVAAL GYAAKAGVPF QQGLIRSHYV GRTFIEPSQK IRDFGVKLKL SPVHAVLEGK 

       430        440        450        460        470        480 
RVVVVDDSIV RGTTSSKIVR LLKEAGAKEV HMRIACPPIV ASCYYGVDTP SSEELISNRM 

       490        500        510        520        530        540 
SVEEIRKFIG SDSLAFLPLD KLKTLLGDDA LNYCYACFSG KYPVEPEELQ MKRLGVAHFN 

       550        560 
WDDDFNGNFE SIDVGGWVTN QDGFKIGSV 

« Hide

References

[1]"Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
Kim J.H., Delauney A.J., Verma D.P.S.
Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Prize.
Tissue: Root nodule.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L23833 mRNA. Translation: AAA73943.1.
PIRT07158.
RefSeqNP_001238270.1. NM_001251341.1.
UniGeneGma.47.

3D structure databases

ProteinModelPortalP52418.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

MEROPSC44.001.

Proteomic databases

ProMEXP52418.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID548065.
KEGGgmx:548065.

Enzyme and pathway databases

BioCycRETL1328306-WGS:GSTH-1440-MONOMER.
UniPathwayUPA00074; UER00124.

Gene expression databases

GenevestigatorP52418.

Family and domain databases

Gene3D3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsTIGR01134. purF. 1 hit.
PROSITEPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry namePUR1_SOYBN
AccessionPrimary (citable) accession number: P52418
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways