Amidophosphoribosyltransferase, chloroplastic precursor

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P52418 (PUR1_SOYBN) Reviewed, UniProtKB/Swiss-Prot

Last modified April 3, 2013. Version 84. History...

Clusters with 100%, 90%, 50% identity |

Documents (2) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Amidophosphoribosyltransferase, chloroplastic
Short name=ATase
EC=2.4.2.14

Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name=GPAT

Gene names

Name:

PUR1

Organism

Glycine max (Soybean) (Glycine hispida) [Reference proteome]

Taxonomic identifier

3847 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › fabids › Fabales › Fabaceae › Papilionoideae › Phaseoleae › Glycine

Protein attributes

Sequence length	569 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Catalytic activity	5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H₂O.
Cofactor	Binds 1 magnesium ion per subunit By similarity. Binds 1 4Fe-4S cluster per subunit By similarity.
Pathway	Purine metabolism; IMP biosynthesis via de novo pathway; N(1)-(5-phospho-D-ribosyl)glycinamide from 5-phospho-alpha-D-ribose 1-diphosphate: step 1/2.
Subcellular location	Plastid › chloroplast.
Sequence similarities	In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family. Contains 1 glutamine amidotransferase type-2 domain.

Ontologies

Keywords
Biological process	Purine biosynthesis
Cellular component	Chloroplast Plastid
Domain	Glutamine amidotransferase Transit peptide
Ligand	4Fe-4S Iron Iron-sulfur Magnesium Metal-binding
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	'de novo' IMP biosynthetic process Inferred from electronic annotation. Source: UniProtKB-UniPathway glutamine metabolic process Inferred from electronic annotation. Source: UniProtKB-KW nucleoside metabolic process Inferred from electronic annotation. Source: InterPro purine nucleobase biosynthetic process Inferred from electronic annotation. Source: InterPro
Cellular_component	chloroplast Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW amidophosphoribosyltransferase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Transit peptide

1 – 80

Chloroplast Potential

Chain

81 – 569

489

Amidophosphoribosyltransferase, chloroplastic

PRO_0000029289

Regions

Domain

81 – 301

221

Glutamine amidotransferase type-2

Sites

Active site

For GATase activity By similarity

Metal binding

317

Iron-sulfur (4Fe-4S) By similarity

Metal binding

364

Magnesium By similarity

Metal binding

426

Magnesium By similarity

Metal binding

427

Magnesium By similarity

Metal binding

463

Iron-sulfur (4Fe-4S) By similarity

Metal binding

514

Iron-sulfur (4Fe-4S) By similarity

Metal binding

517

Iron-sulfur (4Fe-4S) By similarity

Sequences

Sequence

Length

Mass (Da)

Tools

P52418 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 33E36AB22F616491
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FASTA

569

62,205

        10         20         30         40         50         60 
MAAASNLSTL SSSSLSKPSS SFPFRNTPTN NNASFLHNKS LPNQNSLSHK LSSPLPLACN 

        70         80         90        100        110        120 
PKNQNTCVFF DDEDQKPREE CGVVGIYGDP EASRLCSLAL HALQHRGQEG AGIVAVHDNH 

       130        140        150        160        170        180 
LQSVTGVGLV SDVFEQSKLS RLPGTSAIGH VRYSTAGQSM LKNVQPFLAD YRFAAVAVAH 

       190        200        210        220        230        240 
NGNFVNYRSL RARLEHNNGS IFNTTSDTEV VLHLIATSKH RPFLLRIVDA CEHLQGAYSL 

       250        260        270        280        290        300 
VFVTEDKLVA VRDPFGFRPL VMGRRTNGAV VLASETCALD LIEATYEREV YPGEVIVVDH 

       310        320        330        340        350        360 
TGIQSLCLVS HPEPKQCIFE HIYFALPNSV VFGRSVYESR KKFGEILASE SPVECDVVIA 

       370        380        390        400        410        420 
VPDSGVVAAL GYAAKAGVPF QQGLIRSHYV GRTFIEPSQK IRDFGVKLKL SPVHAVLEGK 

       430        440        450        460        470        480 
RVVVVDDSIV RGTTSSKIVR LLKEAGAKEV HMRIACPPIV ASCYYGVDTP SSEELISNRM 

       490        500        510        520        530        540 
SVEEIRKFIG SDSLAFLPLD KLKTLLGDDA LNYCYACFSG KYPVEPEELQ MKRLGVAHFN 

       550        560 
WDDDFNGNFE SIDVGGWVTN QDGFKIGSV

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References

[1]

"Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
Kim J.H., Delauney A.J., Verma D.P.S.
Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Prize.
Tissue: Root nodule.

Cross-references

Sequence databases
EMBL GenBank DDBJ	L23833 mRNA. Translation: AAA73943.1.
PIR	T07158.
RefSeq	NP_001238270.1. NM_001251341.1.
UniGene	Gma.47.
3D structure databases
ProteinModelPortal	P52418.
ModBase	Search...
Protein family/group databases
MEROPS	C44.001.
Proteomic databases
ProMEX	P52418.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	548065.
KEGG	gmx:548065.
Enzyme and pathway databases
UniPathway	UPA00074; UER00124.
Gene expression databases
Genevestigator	P52418.
Family and domain databases
InterPro	IPR005854. Amd_phspho_trans. IPR017932. GATase_2_dom. IPR000583. GATase_dom. IPR000836. PRibTrfase_dom. [Graphical view]
Pfam	PF13537. GATase_7. 1 hit. PF00156. Pribosyltran. 1 hit. [Graphical view]
PIRSF	PIRSF000485. Amd_phspho_trans. 1 hit.
TIGRFAMs	TIGR01134. purF. 1 hit.
PROSITE	PS51278. GATASE_TYPE_2. 1 hit. PS00103. PUR_PYR_PR_TRANSFER. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PUR1_SOYBN

Accession

Primary (citable) accession number: P52418

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 84 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program

Relevant documents

PATHWAY comments

Index of metabolic and biosynthesis pathways

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein family/group databases

Proteomic databases

Protocols and materials databases

Genome annotation databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Entry information

Relevant documents