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P52418

- PUR1_SOYBN

UniProt

P52418 - PUR1_SOYBN

Protein

Amidophosphoribosyltransferase, chloroplastic

Gene

PUR1

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 91 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Catalytic activityi

    5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

    Cofactori

    Binds 1 magnesium ion per subunit.By similarity
    Binds 1 4Fe-4S cluster per subunit.By similarity

    Pathwayi

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei81 – 811For GATase activityBy similarity
    Metal bindingi317 – 3171Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi364 – 3641MagnesiumBy similarity
    Metal bindingi426 – 4261MagnesiumBy similarity
    Metal bindingi427 – 4271MagnesiumBy similarity
    Metal bindingi463 – 4631Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi514 – 5141Iron-sulfur (4Fe-4S)By similarity
    Metal bindingi517 – 5171Iron-sulfur (4Fe-4S)By similarity

    GO - Molecular functioni

    1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
    2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
    3. metal ion binding Source: UniProtKB-KW

    GO - Biological processi

    1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
    2. glutamine metabolic process Source: UniProtKB-KW
    3. nucleoside metabolic process Source: InterPro
    4. purine nucleobase biosynthetic process Source: InterPro

    Keywords - Molecular functioni

    Glycosyltransferase, Transferase

    Keywords - Biological processi

    Purine biosynthesis

    Keywords - Ligandi

    4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

    Enzyme and pathway databases

    BioCyciRETL1328306-WGS:GSTH-1440-MONOMER.
    UniPathwayiUPA00074; UER00124.

    Protein family/group databases

    MEROPSiC44.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Amidophosphoribosyltransferase, chloroplastic (EC:2.4.2.14)
    Short name:
    ATase
    Alternative name(s):
    Glutamine phosphoribosylpyrophosphate amidotransferase
    Short name:
    GPAT
    Gene namesi
    Name:PUR1
    OrganismiGlycine max (Soybean) (Glycine hispida)
    Taxonomic identifieri3847 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
    ProteomesiUP000008827: Unplaced

    Subcellular locationi

    GO - Cellular componenti

    1. chloroplast Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 8080ChloroplastSequence AnalysisAdd
    BLAST
    Chaini81 – 569489Amidophosphoribosyltransferase, chloroplasticPRO_0000029289Add
    BLAST

    Proteomic databases

    ProMEXiP52418.

    Expressioni

    Gene expression databases

    GenevestigatoriP52418.

    Structurei

    3D structure databases

    ProteinModelPortaliP52418.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini81 – 301221Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
    BLAST

    Sequence similaritiesi

    In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
    Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Glutamine amidotransferase, Transit peptide

    Phylogenomic databases

    KOiK00764.

    Family and domain databases

    Gene3Di3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProiIPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view]
    PfamiPF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view]
    PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMiSSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsiTIGR01134. purF. 1 hit.
    PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52418-1 [UniParc]FASTAAdd to Basket

    « Hide

    MAAASNLSTL SSSSLSKPSS SFPFRNTPTN NNASFLHNKS LPNQNSLSHK    50
    LSSPLPLACN PKNQNTCVFF DDEDQKPREE CGVVGIYGDP EASRLCSLAL 100
    HALQHRGQEG AGIVAVHDNH LQSVTGVGLV SDVFEQSKLS RLPGTSAIGH 150
    VRYSTAGQSM LKNVQPFLAD YRFAAVAVAH NGNFVNYRSL RARLEHNNGS 200
    IFNTTSDTEV VLHLIATSKH RPFLLRIVDA CEHLQGAYSL VFVTEDKLVA 250
    VRDPFGFRPL VMGRRTNGAV VLASETCALD LIEATYEREV YPGEVIVVDH 300
    TGIQSLCLVS HPEPKQCIFE HIYFALPNSV VFGRSVYESR KKFGEILASE 350
    SPVECDVVIA VPDSGVVAAL GYAAKAGVPF QQGLIRSHYV GRTFIEPSQK 400
    IRDFGVKLKL SPVHAVLEGK RVVVVDDSIV RGTTSSKIVR LLKEAGAKEV 450
    HMRIACPPIV ASCYYGVDTP SSEELISNRM SVEEIRKFIG SDSLAFLPLD 500
    KLKTLLGDDA LNYCYACFSG KYPVEPEELQ MKRLGVAHFN WDDDFNGNFE 550
    SIDVGGWVTN QDGFKIGSV 569
    Length:569
    Mass (Da):62,205
    Last modified:October 1, 1996 - v1
    Checksum:i33E36AB22F616491
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23833 mRNA. Translation: AAA73943.1.
    PIRiT07158.
    RefSeqiNP_001238270.1. NM_001251341.1.
    UniGeneiGma.47.

    Genome annotation databases

    GeneIDi548065.
    KEGGigmx:548065.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    L23833 mRNA. Translation: AAA73943.1 .
    PIRi T07158.
    RefSeqi NP_001238270.1. NM_001251341.1.
    UniGenei Gma.47.

    3D structure databases

    ProteinModelPortali P52418.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi C44.001.

    Proteomic databases

    ProMEXi P52418.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    GeneIDi 548065.
    KEGGi gmx:548065.

    Phylogenomic databases

    KOi K00764.

    Enzyme and pathway databases

    UniPathwayi UPA00074 ; UER00124 .
    BioCyci RETL1328306-WGS:GSTH-1440-MONOMER.

    Gene expression databases

    Genevestigatori P52418.

    Family and domain databases

    Gene3Di 3.40.50.2020. 1 hit.
    3.60.20.10. 1 hit.
    InterProi IPR005854. Amd_phspho_trans.
    IPR017932. GATase_2_dom.
    IPR000583. GATase_dom.
    IPR029055. Ntn_hydrolases_N.
    IPR000836. PRibTrfase_dom.
    IPR029057. PRTase-like.
    [Graphical view ]
    Pfami PF13537. GATase_7. 1 hit.
    PF00156. Pribosyltran. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
    SUPFAMi SSF53271. SSF53271. 1 hit.
    SSF56235. SSF56235. 1 hit.
    TIGRFAMsi TIGR01134. purF. 1 hit.
    PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
    PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
      Kim J.H., Delauney A.J., Verma D.P.S.
      Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
      Strain: cv. Prize.
      Tissue: Root nodule.

    Entry informationi

    Entry nameiPUR1_SOYBN
    AccessioniPrimary (citable) accession number: P52418
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 91 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. PATHWAY comments
      Index of metabolic and biosynthesis pathways
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3