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P52418

- PUR1_SOYBN

UniProt

P52418 - PUR1_SOYBN

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Protein

Amidophosphoribosyltransferase, chloroplastic

Gene
PUR1
Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 3 out of 5 - Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit By similarity.
Binds 1 4Fe-4S cluster per subunit By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811For GATase activity By similarity
Metal bindingi317 – 3171Iron-sulfur (4Fe-4S) By similarity
Metal bindingi364 – 3641Magnesium By similarity
Metal bindingi426 – 4261Magnesium By similarity
Metal bindingi427 – 4271Magnesium By similarity
Metal bindingi463 – 4631Iron-sulfur (4Fe-4S) By similarity
Metal bindingi514 – 5141Iron-sulfur (4Fe-4S) By similarity
Metal bindingi517 – 5171Iron-sulfur (4Fe-4S) By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: InterPro
  4. purine nucleobase biosynthetic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1440-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase, chloroplastic (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPAT
Gene namesi
Name:PUR1
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8080Chloroplast Reviewed predictionAdd
BLAST
Chaini81 – 569489Amidophosphoribosyltransferase, chloroplasticPRO_0000029289Add
BLAST

Proteomic databases

ProMEXiP52418.

Expressioni

Gene expression databases

GenevestigatoriP52418.

Structurei

3D structure databases

ProteinModelPortaliP52418.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 301221Glutamine amidotransferase type-2Add
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.

Keywords - Domaini

Glutamine amidotransferase, Transit peptide

Phylogenomic databases

KOiK00764.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52418-1 [UniParc]FASTAAdd to Basket

« Hide

MAAASNLSTL SSSSLSKPSS SFPFRNTPTN NNASFLHNKS LPNQNSLSHK    50
LSSPLPLACN PKNQNTCVFF DDEDQKPREE CGVVGIYGDP EASRLCSLAL 100
HALQHRGQEG AGIVAVHDNH LQSVTGVGLV SDVFEQSKLS RLPGTSAIGH 150
VRYSTAGQSM LKNVQPFLAD YRFAAVAVAH NGNFVNYRSL RARLEHNNGS 200
IFNTTSDTEV VLHLIATSKH RPFLLRIVDA CEHLQGAYSL VFVTEDKLVA 250
VRDPFGFRPL VMGRRTNGAV VLASETCALD LIEATYEREV YPGEVIVVDH 300
TGIQSLCLVS HPEPKQCIFE HIYFALPNSV VFGRSVYESR KKFGEILASE 350
SPVECDVVIA VPDSGVVAAL GYAAKAGVPF QQGLIRSHYV GRTFIEPSQK 400
IRDFGVKLKL SPVHAVLEGK RVVVVDDSIV RGTTSSKIVR LLKEAGAKEV 450
HMRIACPPIV ASCYYGVDTP SSEELISNRM SVEEIRKFIG SDSLAFLPLD 500
KLKTLLGDDA LNYCYACFSG KYPVEPEELQ MKRLGVAHFN WDDDFNGNFE 550
SIDVGGWVTN QDGFKIGSV 569
Length:569
Mass (Da):62,205
Last modified:October 1, 1996 - v1
Checksum:i33E36AB22F616491
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23833 mRNA. Translation: AAA73943.1.
PIRiT07158.
RefSeqiNP_001238270.1. NM_001251341.1.
UniGeneiGma.47.

Genome annotation databases

GeneIDi548065.
KEGGigmx:548065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23833 mRNA. Translation: AAA73943.1 .
PIRi T07158.
RefSeqi NP_001238270.1. NM_001251341.1.
UniGenei Gma.47.

3D structure databases

ProteinModelPortali P52418.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.001.

Proteomic databases

ProMEXi P52418.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 548065.
KEGGi gmx:548065.

Phylogenomic databases

KOi K00764.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci RETL1328306-WGS:GSTH-1440-MONOMER.

Gene expression databases

Genevestigatori P52418.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
    Kim J.H., Delauney A.J., Verma D.P.S.
    Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Prize.
    Tissue: Root nodule.

Entry informationi

Entry nameiPUR1_SOYBN
AccessioniPrimary (citable) accession number: P52418
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 3, 2014
This is version 90 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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