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P52418

- PUR1_SOYBN

UniProt

P52418 - PUR1_SOYBN

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Protein

Amidophosphoribosyltransferase, chloroplastic

Gene

PUR1

Organism
Glycine max (Soybean) (Glycine hispida)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli

Functioni

Catalytic activityi

5-phospho-beta-D-ribosylamine + diphosphate + L-glutamate = L-glutamine + 5-phospho-alpha-D-ribose 1-diphosphate + H2O.

Cofactori

Binds 1 magnesium ion per subunit.By similarity
Binds 1 4Fe-4S cluster per subunit.By similarity

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei81 – 811NucleophilePROSITE-ProRule annotation
Metal bindingi317 – 3171Iron-sulfur (4Fe-4S)By similarity
Metal bindingi364 – 3641MagnesiumBy similarity
Metal bindingi426 – 4261MagnesiumBy similarity
Metal bindingi427 – 4271MagnesiumBy similarity
Metal bindingi463 – 4631Iron-sulfur (4Fe-4S)By similarity
Metal bindingi514 – 5141Iron-sulfur (4Fe-4S)By similarity
Metal bindingi517 – 5171Iron-sulfur (4Fe-4S)By similarity

GO - Molecular functioni

  1. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  2. amidophosphoribosyltransferase activity Source: UniProtKB-EC
  3. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. 'de novo' IMP biosynthetic process Source: UniProtKB-UniPathway
  2. glutamine metabolic process Source: UniProtKB-KW
  3. nucleoside metabolic process Source: EnsemblPlants/Gramene
  4. purine nucleobase biosynthetic process Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Purine biosynthesis

Keywords - Ligandi

4Fe-4S, Iron, Iron-sulfur, Magnesium, Metal-binding

Enzyme and pathway databases

BioCyciRETL1328306-WGS:GSTH-1440-MONOMER.
UniPathwayiUPA00074; UER00124.

Protein family/group databases

MEROPSiC44.001.

Names & Taxonomyi

Protein namesi
Recommended name:
Amidophosphoribosyltransferase, chloroplastic (EC:2.4.2.14)
Short name:
ATase
Alternative name(s):
Glutamine phosphoribosylpyrophosphate amidotransferase
Short name:
GPAT
Gene namesi
Name:PUR1
OrganismiGlycine max (Soybean) (Glycine hispida)
Taxonomic identifieri3847 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsfabidsFabalesFabaceaePapilionoideaePhaseoleaeGlycineSoja
ProteomesiUP000008827: Unplaced

Subcellular locationi

GO - Cellular componenti

  1. chloroplast stroma Source: EnsemblPlants/Gramene
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 8080ChloroplastSequence AnalysisAdd
BLAST
Chaini81 – 569489Amidophosphoribosyltransferase, chloroplasticPRO_0000029289Add
BLAST

Proteomic databases

ProMEXiP52418.

Expressioni

Gene expression databases

GenevestigatoriP52418.

Structurei

3D structure databases

ProteinModelPortaliP52418.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini81 – 301221Glutamine amidotransferase type-2PROSITE-ProRule annotationAdd
BLAST

Sequence similaritiesi

In the C-terminal section; belongs to the purine/pyrimidine phosphoribosyltransferase family.Curated
Contains 1 glutamine amidotransferase type-2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Glutamine amidotransferase, Transit peptide

Phylogenomic databases

InParanoidiP52418.
KOiK00764.

Family and domain databases

Gene3Di3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPiMF_01931. PurF.
InterProiIPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view]
PfamiPF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view]
PIRSFiPIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMiSSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsiTIGR01134. purF. 1 hit.
PROSITEiPS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52418-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MAAASNLSTL SSSSLSKPSS SFPFRNTPTN NNASFLHNKS LPNQNSLSHK
60 70 80 90 100
LSSPLPLACN PKNQNTCVFF DDEDQKPREE CGVVGIYGDP EASRLCSLAL
110 120 130 140 150
HALQHRGQEG AGIVAVHDNH LQSVTGVGLV SDVFEQSKLS RLPGTSAIGH
160 170 180 190 200
VRYSTAGQSM LKNVQPFLAD YRFAAVAVAH NGNFVNYRSL RARLEHNNGS
210 220 230 240 250
IFNTTSDTEV VLHLIATSKH RPFLLRIVDA CEHLQGAYSL VFVTEDKLVA
260 270 280 290 300
VRDPFGFRPL VMGRRTNGAV VLASETCALD LIEATYEREV YPGEVIVVDH
310 320 330 340 350
TGIQSLCLVS HPEPKQCIFE HIYFALPNSV VFGRSVYESR KKFGEILASE
360 370 380 390 400
SPVECDVVIA VPDSGVVAAL GYAAKAGVPF QQGLIRSHYV GRTFIEPSQK
410 420 430 440 450
IRDFGVKLKL SPVHAVLEGK RVVVVDDSIV RGTTSSKIVR LLKEAGAKEV
460 470 480 490 500
HMRIACPPIV ASCYYGVDTP SSEELISNRM SVEEIRKFIG SDSLAFLPLD
510 520 530 540 550
KLKTLLGDDA LNYCYACFSG KYPVEPEELQ MKRLGVAHFN WDDDFNGNFE
560
SIDVGGWVTN QDGFKIGSV
Length:569
Mass (Da):62,205
Last modified:October 1, 1996 - v1
Checksum:i33E36AB22F616491
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23833 mRNA. Translation: AAA73943.1.
PIRiT07158.
RefSeqiNP_001238270.1. NM_001251341.1.
UniGeneiGma.47.

Genome annotation databases

GeneIDi548065.
KEGGigmx:548065.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L23833 mRNA. Translation: AAA73943.1 .
PIRi T07158.
RefSeqi NP_001238270.1. NM_001251341.1.
UniGenei Gma.47.

3D structure databases

ProteinModelPortali P52418.
ModBasei Search...
MobiDBi Search...

Protein family/group databases

MEROPSi C44.001.

Proteomic databases

ProMEXi P52418.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

GeneIDi 548065.
KEGGi gmx:548065.

Phylogenomic databases

InParanoidi P52418.
KOi K00764.

Enzyme and pathway databases

UniPathwayi UPA00074 ; UER00124 .
BioCyci RETL1328306-WGS:GSTH-1440-MONOMER.

Gene expression databases

Genevestigatori P52418.

Family and domain databases

Gene3Di 3.40.50.2020. 1 hit.
3.60.20.10. 1 hit.
HAMAPi MF_01931. PurF.
InterProi IPR005854. Amd_phspho_trans.
IPR017932. GATase_2_dom.
IPR000583. GATase_dom.
IPR029055. Ntn_hydrolases_N.
IPR000836. PRibTrfase_dom.
IPR029057. PRTase-like.
[Graphical view ]
Pfami PF13537. GATase_7. 1 hit.
PF00156. Pribosyltran. 1 hit.
[Graphical view ]
PIRSFi PIRSF000485. Amd_phspho_trans. 1 hit.
SUPFAMi SSF53271. SSF53271. 1 hit.
SSF56235. SSF56235. 1 hit.
TIGRFAMsi TIGR01134. purF. 1 hit.
PROSITEi PS51278. GATASE_TYPE_2. 1 hit.
PS00103. PUR_PYR_PR_TRANSFER. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Control of de novo purine biosynthesis genes in ureide-producing legumes: induction of glutamine phosphoribosylpyrophosphate amidotransferase gene and characterization of its cDNA from soybean and Vigna."
    Kim J.H., Delauney A.J., Verma D.P.S.
    Plant J. 7:77-86(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: cv. Prize.
    Tissue: Root nodule.

Entry informationi

Entry nameiPUR1_SOYBN
AccessioniPrimary (citable) accession number: P52418
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 92 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3