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P52410

- KASC1_ARATH

UniProt

P52410 - KASC1_ARATH

Protein

3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic

Gene

KAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 116 (01 Oct 2014)
      Sequence version 2 (27 Mar 2002)
      Previous versions | rss
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    Functioni

    Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids By similarity.By similarity

    Catalytic activityi

    Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei224 – 2241PROSITE-ProRule annotation

    GO - Molecular functioni

    1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC

    GO - Biological processi

    1. fatty acid biosynthetic process Source: UniProtKB-KW

    Keywords - Molecular functioni

    Acyltransferase, Transferase

    Keywords - Biological processi

    Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

    Enzyme and pathway databases

    BioCyciARA:AT5G46290-MONOMER.
    ARA:GQT-472-MONOMER.
    ARA:GQT-473-MONOMER.
    MetaCyc:AT5G46290-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic (EC:2.3.1.41)
    Alternative name(s):
    Beta-ketoacyl-ACP synthase I
    Short name:
    KAS I
    Gene namesi
    Name:KAS1
    Ordered Locus Names:At5g46290
    ORF Names:MPL12.7
    OrganismiArabidopsis thaliana (Mouse-ear cress)
    Taxonomic identifieri3702 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
    ProteomesiUP000006548: Chromosome 5

    Organism-specific databases

    TAIRiAT5G46290.

    Subcellular locationi

    Plastidchloroplast stroma 4 Publications

    GO - Cellular componenti

    1. chloroplast stroma Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Chloroplast, Plastid

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transit peptidei1 – 4646ChloroplastSequence AnalysisAdd
    BLAST
    Chaini47 – 4734273-oxoacyl-[acyl-carrier-protein] synthase I, chloroplasticPRO_0000000586Add
    BLAST

    Proteomic databases

    PaxDbiP52410.
    PRIDEiP52410.

    Expressioni

    Gene expression databases

    GenevestigatoriP52410.

    Interactioni

    Subunit structurei

    Homodimer.By similarity

    Protein-protein interaction databases

    STRINGi3702.AT5G46290.1-P.

    Structurei

    3D structure databases

    ProteinModelPortaliP52410.
    SMRiP52410. Positions 60-471.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the beta-ketoacyl-ACP synthases family.Curated

    Keywords - Domaini

    Transit peptide

    Phylogenomic databases

    eggNOGiCOG0304.
    HOGENOMiHOG000060166.
    InParanoidiP52410.
    KOiK09458.
    PhylomeDBiP52410.

    Family and domain databases

    Gene3Di3.40.47.10. 2 hits.
    InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view]
    PfamiPF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view]
    SUPFAMiSSF53901. SSF53901. 2 hits.
    TIGRFAMsiTIGR03150. fabF. 1 hit.
    PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: P52410-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

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    MQALQSSSLR ASPPNPLRLP SNRQSHQLIT NARPLRRQQR SFISASASTV    50
    SAPKRETDPK KRVVITGMGL VSVFGNDVDA YYEKLLSGES GISLIDRFDA 100
    SKFPTRFGGQ IRGFSSEGYI DGKNERRLDD CLKYCIVAGK KALESANLGG 150
    DKLNTIDKRK AGVLVGTGMG GLTVFSEGVQ NLIEKGHRRI SPFFIPYAIT 200
    NMGSALLAID LGLMGPNYSI STACATSNYC FYAAANHIRR GEADMMIAGG 250
    TEAAIIPIGL GGFVACRALS QRNDDPQTAS RPWDKARDGF VMGEGAGVLV 300
    MESLEHAMKR GAPIVAEYLG GAVNCDAHHM TDPRADGLGV SSCIERCLED 350
    AGVSPEEVNY INAHATSTLA GDLAEINAIK KVFKSTSGIK INATKSMIGH 400
    CLGAAGGLEA IATVKAINTG WLHPSINQFN PEQAVDFDTV PNEKKQHEVD 450
    VAISNSFGFG GHNSVVAFSA FKP 473
    Length:473
    Mass (Da):50,413
    Last modified:March 27, 2002 - v2
    Checksum:i38CA54382419411C
    GO
    Isoform 2 (identifier: P52410-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         198-252: Missing.

    Show »
    Length:418
    Mass (Da):44,730
    Checksum:i1B656C5985A93752
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti74 – 741F → C in AAC49118. 1 PublicationCurated
    Sequence conflicti238 – 2392IR → NH in AAC49118. 1 PublicationCurated

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei198 – 25255Missing in isoform 2. CuratedVSP_040746Add
    BLAST

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24177 mRNA. Translation: AAC49118.1.
    AB010698 Genomic DNA. Translation: BAB11084.1.
    CP002688 Genomic DNA. Translation: AED95363.1.
    CP002688 Genomic DNA. Translation: AED95364.1.
    AY037261 mRNA. Translation: AAK59862.1.
    AY094005 mRNA. Translation: AAM16266.1.
    AY123979 mRNA. Translation: AAM74493.1.
    AY087843 mRNA. Translation: AAM65396.1.
    RefSeqiNP_001032018.1. NM_001036941.1. [P52410-2]
    NP_199441.1. NM_123998.2. [P52410-1]
    UniGeneiAt.9183.

    Genome annotation databases

    EnsemblPlantsiAT5G46290.1; AT5G46290.1; AT5G46290. [P52410-1]
    GeneIDi834671.
    KEGGiath:AT5G46290.

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U24177 mRNA. Translation: AAC49118.1 .
    AB010698 Genomic DNA. Translation: BAB11084.1 .
    CP002688 Genomic DNA. Translation: AED95363.1 .
    CP002688 Genomic DNA. Translation: AED95364.1 .
    AY037261 mRNA. Translation: AAK59862.1 .
    AY094005 mRNA. Translation: AAM16266.1 .
    AY123979 mRNA. Translation: AAM74493.1 .
    AY087843 mRNA. Translation: AAM65396.1 .
    RefSeqi NP_001032018.1. NM_001036941.1. [P52410-2 ]
    NP_199441.1. NM_123998.2. [P52410-1 ]
    UniGenei At.9183.

    3D structure databases

    ProteinModelPortali P52410.
    SMRi P52410. Positions 60-471.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 3702.AT5G46290.1-P.

    Proteomic databases

    PaxDbi P52410.
    PRIDEi P52410.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblPlantsi AT5G46290.1 ; AT5G46290.1 ; AT5G46290 . [P52410-1 ]
    GeneIDi 834671.
    KEGGi ath:AT5G46290.

    Organism-specific databases

    TAIRi AT5G46290.

    Phylogenomic databases

    eggNOGi COG0304.
    HOGENOMi HOG000060166.
    InParanoidi P52410.
    KOi K09458.
    PhylomeDBi P52410.

    Enzyme and pathway databases

    BioCyci ARA:AT5G46290-MONOMER.
    ARA:GQT-472-MONOMER.
    ARA:GQT-473-MONOMER.
    MetaCyc:AT5G46290-MONOMER.

    Gene expression databases

    Genevestigatori P52410.

    Family and domain databases

    Gene3Di 3.40.47.10. 2 hits.
    InterProi IPR017568. 3-oxoacyl-ACP_synth-2.
    IPR018201. Ketoacyl_synth_AS.
    IPR014031. Ketoacyl_synth_C.
    IPR014030. Ketoacyl_synth_N.
    IPR016039. Thiolase-like.
    IPR016038. Thiolase-like_subgr.
    [Graphical view ]
    Pfami PF00109. ketoacyl-synt. 1 hit.
    PF02801. Ketoacyl-synt_C. 1 hit.
    [Graphical view ]
    SUPFAMi SSF53901. SSF53901. 2 hits.
    TIGRFAMsi TIGR03150. fabF. 1 hit.
    PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Isolation of an Arabidopsis cDNA encoding 3-ketoacyl-acyl carrier protein synthase I.1."
      Millar A.A., Kunst L.
      Plant Gene Register PGR95-027
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
      Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
      DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: cv. Columbia.
    3. The Arabidopsis Information Resource (TAIR)
      Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
      Cited for: GENOME REANNOTATION.
      Strain: cv. Columbia.
    4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
      Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
      , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
      Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
      Strain: cv. Columbia.
    5. "Full-length cDNA from Arabidopsis thaliana."
      Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
      Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    6. "Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis."
      Froehlich J.E., Wilkerson C.G., Ray W.K., McAndrew R.S., Osteryoung K.W., Gage D.A., Phinney B.S.
      J. Proteome Res. 2:413-425(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    8. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
      Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
      PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
    9. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
      Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
      Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
      Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

    Entry informationi

    Entry nameiKASC1_ARATH
    AccessioniPrimary (citable) accession number: P52410
    Secondary accession number(s): Q2V312, Q9FL32
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: March 27, 2002
    Last modified: October 1, 2014
    This is version 116 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Arabidopsis thaliana
      Arabidopsis thaliana: entries and gene names
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3