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Protein

3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic

Gene

KAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids (By similarity).By similarity

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei224PROSITE-ProRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAcyltransferase, Transferase
Biological processFatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT5G46290-MONOMER.
MetaCyc:AT5G46290-MONOMER.
BRENDAi2.3.1.41. 399.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl-ACP synthase I
Short name:
KAS I
Gene namesi
Name:KAS1
Ordered Locus Names:At5g46290
ORF Names:MPL12.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 5

Organism-specific databases

AraportiAT5G46290.

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cell wall Cytoskeleton Vacuole Chloroplast Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertion Graphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 46ChloroplastSequence analysisAdd BLAST46
ChainiPRO_000000058647 – 4733-oxoacyl-[acyl-carrier-protein] synthase I, chloroplasticAdd BLAST427

Proteomic databases

PaxDbiP52410.
PRIDEiP52410.

Expressioni

Gene expression databases

ExpressionAtlasiP52410. baseline and differential.
GenevisibleiP52410. AT.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G46290.3.

Structurei

3D structure databases

ProteinModelPortaliP52410.
SMRiP52410.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG1394. Eukaryota.
COG0304. LUCA.
HOGENOMiHOG000060166.
InParanoidiP52410.
KOiK09458.
PhylomeDBiP52410.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiView protein in InterPro
IPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR020841. PKS_Beta-ketoAc_synthase_dom.
IPR016039. Thiolase-like.
PfamiView protein in Pfam
PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
SMARTiView protein in SMART
SM00825. PKS_KS. 1 hit.
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiView protein in PROSITE
PS00606. B_KETOACYL_SYNTHASE. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 1 (identifier: P52410-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQALQSSSLR ASPPNPLRLP SNRQSHQLIT NARPLRRQQR SFISASASTV
60 70 80 90 100
SAPKRETDPK KRVVITGMGL VSVFGNDVDA YYEKLLSGES GISLIDRFDA
110 120 130 140 150
SKFPTRFGGQ IRGFSSEGYI DGKNERRLDD CLKYCIVAGK KALESANLGG
160 170 180 190 200
DKLNTIDKRK AGVLVGTGMG GLTVFSEGVQ NLIEKGHRRI SPFFIPYAIT
210 220 230 240 250
NMGSALLAID LGLMGPNYSI STACATSNYC FYAAANHIRR GEADMMIAGG
260 270 280 290 300
TEAAIIPIGL GGFVACRALS QRNDDPQTAS RPWDKARDGF VMGEGAGVLV
310 320 330 340 350
MESLEHAMKR GAPIVAEYLG GAVNCDAHHM TDPRADGLGV SSCIERCLED
360 370 380 390 400
AGVSPEEVNY INAHATSTLA GDLAEINAIK KVFKSTSGIK INATKSMIGH
410 420 430 440 450
CLGAAGGLEA IATVKAINTG WLHPSINQFN PEQAVDFDTV PNEKKQHEVD
460 470
VAISNSFGFG GHNSVVAFSA FKP
Length:473
Mass (Da):50,413
Last modified:March 27, 2002 - v2
Checksum:i38CA54382419411C
GO
Isoform 2 (identifier: P52410-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-252: Missing.

Show »
Length:418
Mass (Da):44,730
Checksum:i1B656C5985A93752
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti74F → C in AAC49118 (Ref. 1) Curated1
Sequence conflicti238 – 239IR → NH in AAC49118 (Ref. 1) Curated2

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_040746198 – 252Missing in isoform 2. CuratedAdd BLAST55

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U24177 mRNA. Translation: AAC49118.1.
AB010698 Genomic DNA. Translation: BAB11084.1.
CP002688 Genomic DNA. Translation: AED95363.1.
CP002688 Genomic DNA. Translation: AED95364.1.
AY037261 mRNA. Translation: AAK59862.1.
AY094005 mRNA. Translation: AAM16266.1.
AY123979 mRNA. Translation: AAM74493.1.
AY087843 mRNA. Translation: AAM65396.1.
RefSeqiNP_001032018.1. NM_001036941.1. [P52410-2]
NP_199441.1. NM_123998.3. [P52410-1]
UniGeneiAt.9183.

Genome annotation databases

EnsemblPlantsiAT5G46290.1; AT5G46290.1; AT5G46290. [P52410-1]
AT5G46290.2; AT5G46290.2; AT5G46290. [P52410-2]
GeneIDi834671.
GrameneiAT5G46290.1; AT5G46290.1; AT5G46290.
AT5G46290.2; AT5G46290.2; AT5G46290.
KEGGiath:AT5G46290.

Keywords - Coding sequence diversityi

Alternative splicing

Similar proteinsi

Entry informationi

Entry nameiKASC1_ARATH
AccessioniPrimary (citable) accession number: P52410
Secondary accession number(s): Q2V312, Q9FL32
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 27, 2002
Last modified: November 22, 2017
This is version 135 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families