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P52410

- KASC1_ARATH

UniProt

P52410 - KASC1_ARATH

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Protein

3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic

Gene

KAS1

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli

Functioni

Catalyzes the condensation reaction of fatty acid synthesis by the addition to an acyl acceptor of two carbons from malonyl-ACP. Specific for elongation from C-10 to unsaturated C-16 and C-18 fatty acids (By similarity).By similarity

Catalytic activityi

Acyl-[acyl-carrier-protein] + malonyl-[acyl-carrier-protein] = 3-oxoacyl-[acyl-carrier-protein] + CO2 + [acyl-carrier-protein].

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei224 – 2241PROSITE-ProRule annotation

GO - Molecular functioni

  1. 3-oxoacyl-[acyl-carrier-protein] synthase activity Source: UniProtKB-EC

GO - Biological processi

  1. fatty acid biosynthetic process Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Acyltransferase, Transferase

Keywords - Biological processi

Fatty acid biosynthesis, Fatty acid metabolism, Lipid biosynthesis, Lipid metabolism

Enzyme and pathway databases

BioCyciARA:AT5G46290-MONOMER.
ARA:GQT-472-MONOMER.
ARA:GQT-473-MONOMER.
MetaCyc:AT5G46290-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
3-oxoacyl-[acyl-carrier-protein] synthase I, chloroplastic (EC:2.3.1.41)
Alternative name(s):
Beta-ketoacyl-ACP synthase I
Short name:
KAS I
Gene namesi
Name:KAS1
Ordered Locus Names:At5g46290
ORF Names:MPL12.7
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
ProteomesiUP000006548: Chromosome 5

Organism-specific databases

TAIRiAT5G46290.

Subcellular locationi

Plastidchloroplast stroma 4 Publications

GO - Cellular componenti

  1. chloroplast Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Chloroplast, Plastid

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 4646ChloroplastSequence AnalysisAdd
BLAST
Chaini47 – 4734273-oxoacyl-[acyl-carrier-protein] synthase I, chloroplasticPRO_0000000586Add
BLAST

Proteomic databases

PaxDbiP52410.
PRIDEiP52410.

Expressioni

Gene expression databases

ExpressionAtlasiP52410. baseline and differential.
GenevestigatoriP52410.

Interactioni

Subunit structurei

Homodimer.By similarity

Protein-protein interaction databases

STRINGi3702.AT5G46290.1-P.

Structurei

3D structure databases

ProteinModelPortaliP52410.
SMRiP52410. Positions 60-471.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the beta-ketoacyl-ACP synthases family.Curated

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiCOG0304.
HOGENOMiHOG000060166.
InParanoidiP52410.
KOiK09458.
PhylomeDBiP52410.

Family and domain databases

Gene3Di3.40.47.10. 2 hits.
InterProiIPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view]
PfamiPF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view]
SUPFAMiSSF53901. SSF53901. 2 hits.
TIGRFAMsiTIGR03150. fabF. 1 hit.
PROSITEiPS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. Align

Isoform 1 (identifier: P52410-1) [UniParc]FASTAAdd to Basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MQALQSSSLR ASPPNPLRLP SNRQSHQLIT NARPLRRQQR SFISASASTV
60 70 80 90 100
SAPKRETDPK KRVVITGMGL VSVFGNDVDA YYEKLLSGES GISLIDRFDA
110 120 130 140 150
SKFPTRFGGQ IRGFSSEGYI DGKNERRLDD CLKYCIVAGK KALESANLGG
160 170 180 190 200
DKLNTIDKRK AGVLVGTGMG GLTVFSEGVQ NLIEKGHRRI SPFFIPYAIT
210 220 230 240 250
NMGSALLAID LGLMGPNYSI STACATSNYC FYAAANHIRR GEADMMIAGG
260 270 280 290 300
TEAAIIPIGL GGFVACRALS QRNDDPQTAS RPWDKARDGF VMGEGAGVLV
310 320 330 340 350
MESLEHAMKR GAPIVAEYLG GAVNCDAHHM TDPRADGLGV SSCIERCLED
360 370 380 390 400
AGVSPEEVNY INAHATSTLA GDLAEINAIK KVFKSTSGIK INATKSMIGH
410 420 430 440 450
CLGAAGGLEA IATVKAINTG WLHPSINQFN PEQAVDFDTV PNEKKQHEVD
460 470
VAISNSFGFG GHNSVVAFSA FKP
Length:473
Mass (Da):50,413
Last modified:March 27, 2002 - v2
Checksum:i38CA54382419411C
GO
Isoform 2 (identifier: P52410-2) [UniParc]FASTAAdd to Basket

The sequence of this isoform differs from the canonical sequence as follows:
     198-252: Missing.

Show »
Length:418
Mass (Da):44,730
Checksum:i1B656C5985A93752
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti74 – 741F → C in AAC49118. 1 PublicationCurated
Sequence conflicti238 – 2392IR → NH in AAC49118. 1 PublicationCurated

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei198 – 25255Missing in isoform 2. CuratedVSP_040746Add
BLAST

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24177 mRNA. Translation: AAC49118.1.
AB010698 Genomic DNA. Translation: BAB11084.1.
CP002688 Genomic DNA. Translation: AED95363.1.
CP002688 Genomic DNA. Translation: AED95364.1.
AY037261 mRNA. Translation: AAK59862.1.
AY094005 mRNA. Translation: AAM16266.1.
AY123979 mRNA. Translation: AAM74493.1.
AY087843 mRNA. Translation: AAM65396.1.
RefSeqiNP_001032018.1. NM_001036941.1. [P52410-2]
NP_199441.1. NM_123998.2. [P52410-1]
UniGeneiAt.9183.

Genome annotation databases

EnsemblPlantsiAT5G46290.1; AT5G46290.1; AT5G46290. [P52410-1]
GeneIDi834671.
KEGGiath:AT5G46290.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
U24177 mRNA. Translation: AAC49118.1 .
AB010698 Genomic DNA. Translation: BAB11084.1 .
CP002688 Genomic DNA. Translation: AED95363.1 .
CP002688 Genomic DNA. Translation: AED95364.1 .
AY037261 mRNA. Translation: AAK59862.1 .
AY094005 mRNA. Translation: AAM16266.1 .
AY123979 mRNA. Translation: AAM74493.1 .
AY087843 mRNA. Translation: AAM65396.1 .
RefSeqi NP_001032018.1. NM_001036941.1. [P52410-2 ]
NP_199441.1. NM_123998.2. [P52410-1 ]
UniGenei At.9183.

3D structure databases

ProteinModelPortali P52410.
SMRi P52410. Positions 60-471.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 3702.AT5G46290.1-P.

Proteomic databases

PaxDbi P52410.
PRIDEi P52410.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblPlantsi AT5G46290.1 ; AT5G46290.1 ; AT5G46290 . [P52410-1 ]
GeneIDi 834671.
KEGGi ath:AT5G46290.

Organism-specific databases

TAIRi AT5G46290.

Phylogenomic databases

eggNOGi COG0304.
HOGENOMi HOG000060166.
InParanoidi P52410.
KOi K09458.
PhylomeDBi P52410.

Enzyme and pathway databases

BioCyci ARA:AT5G46290-MONOMER.
ARA:GQT-472-MONOMER.
ARA:GQT-473-MONOMER.
MetaCyc:AT5G46290-MONOMER.

Gene expression databases

ExpressionAtlasi P52410. baseline and differential.
Genevestigatori P52410.

Family and domain databases

Gene3Di 3.40.47.10. 2 hits.
InterProi IPR017568. 3-oxoacyl-ACP_synth-2.
IPR018201. Ketoacyl_synth_AS.
IPR014031. Ketoacyl_synth_C.
IPR014030. Ketoacyl_synth_N.
IPR016039. Thiolase-like.
IPR016038. Thiolase-like_subgr.
[Graphical view ]
Pfami PF00109. ketoacyl-synt. 1 hit.
PF02801. Ketoacyl-synt_C. 1 hit.
[Graphical view ]
SUPFAMi SSF53901. SSF53901. 2 hits.
TIGRFAMsi TIGR03150. fabF. 1 hit.
PROSITEi PS00606. B_KETOACYL_SYNTHASE. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Isolation of an Arabidopsis cDNA encoding 3-ketoacyl-acyl carrier protein synthase I.1."
    Millar A.A., Kunst L.
    Plant Gene Register PGR95-027
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  2. "Structural analysis of Arabidopsis thaliana chromosome 5. V. Sequence features of the regions of 1,381,565 bp covered by twenty one physically assigned P1 and TAC clones."
    Kaneko T., Kotani H., Nakamura Y., Sato S., Asamizu E., Miyajima N., Tabata S.
    DNA Res. 5:131-145(1998) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "Empirical analysis of transcriptional activity in the Arabidopsis genome."
    Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M., Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G., Liu S.X., Lam B., Sakano H., Wu T., Yu G.
    , Miranda M., Quach H.L., Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C., Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J., Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A., Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C., Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X., Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M., Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K., Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A., Ecker J.R.
    Science 302:842-846(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
    Strain: cv. Columbia.
  5. "Full-length cDNA from Arabidopsis thaliana."
    Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B., Feldmann K.A.
    Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
  6. "Proteomic study of the Arabidopsis thaliana chloroplastic envelope membrane utilizing alternatives to traditional two-dimensional electrophoresis."
    Froehlich J.E., Wilkerson C.G., Ray W.K., McAndrew R.S., Osteryoung K.W., Gage D.A., Phinney B.S.
    J. Proteome Res. 2:413-425(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  7. Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  8. "Sorting signals, N-terminal modifications and abundance of the chloroplast proteome."
    Zybailov B., Rutschow H., Friso G., Rudella A., Emanuelsson O., Sun Q., van Wijk K.J.
    PLoS ONE 3:E1994-E1994(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].
  9. "AT_CHLORO, a comprehensive chloroplast proteome database with subplastidial localization and curated information on envelope proteins."
    Ferro M., Brugiere S., Salvi D., Seigneurin-Berny D., Court M., Moyet L., Ramus C., Miras S., Mellal M., Le Gall S., Kieffer-Jaquinod S., Bruley C., Garin J., Joyard J., Masselon C., Rolland N.
    Mol. Cell. Proteomics 9:1063-1084(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: SUBCELLULAR LOCATION [LARGE SCALE ANALYSIS].

Entry informationi

Entry nameiKASC1_ARATH
AccessioniPrimary (citable) accession number: P52410
Secondary accession number(s): Q2V312, Q9FL32
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: March 27, 2002
Last modified: October 29, 2014
This is version 117 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3