ID   E13B_PRUPE              Reviewed;         350 AA.
AC   P52408;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-1996, sequence version 1.
DT   27-MAR-2024, entry version 89.
DE   RecName: Full=Glucan endo-1,3-beta-glucosidase, basic isoform;
DE            EC=3.2.1.39;
DE   AltName: Full=(1->3)-beta-glucan endohydrolase;
DE            Short=(1->3)-beta-glucanase;
DE   AltName: Full=Beta-1,3-endoglucanase;
DE   AltName: Full=PpGns1;
DE   Flags: Precursor;
GN   Name=GNS1;
OS   Prunus persica (Peach) (Amygdalus persica).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; fabids; Rosales; Rosaceae; Amygdaloideae; Amygdaleae; Prunus.
OX   NCBI_TaxID=3760;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=11405630; DOI=10.1007/s004380000435;
RA   Thimmapuram J., Ko T.S., Korban S.S.;
RT   "Characterization and expression of beta-1,3-glucanase genes in peach.";
RL   Mol. Genet. Genomics 265:469-479(2001).
CC   -!- FUNCTION: Is thought to be an important plant defense-related product
CC       against fungal pathogens.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-
CC         beta-D-glucans.; EC=3.2.1.39;
CC   -!- SIMILARITY: Belongs to the glycosyl hydrolase 17 family. {ECO:0000305}.
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DR   EMBL; U49454; AAA92013.1; -; Genomic_DNA.
DR   RefSeq; XP_007203676.1; XM_007203614.1.
DR   AlphaFoldDB; P52408; -.
DR   SMR; P52408; -.
DR   CAZy; GH17; Glycoside Hydrolase Family 17.
DR   EnsemblPlants; ONH95098; ONH95098; PRUPE_7G052000.
DR   GeneID; 18771149; -.
DR   Gramene; ONH95098; ONH95098; PRUPE_7G052000.
DR   KEGG; pper:18771149; -.
DR   eggNOG; ENOG502QQ3M; Eukaryota.
DR   OrthoDB; 46602at2759; -.
DR   PhylomeDB; P52408; -.
DR   GO; GO:0042973; F:glucan endo-1,3-beta-D-glucosidase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005975; P:carbohydrate metabolic process; IEA:InterPro.
DR   GO; GO:0006952; P:defense response; IEA:UniProtKB-KW.
DR   GO; GO:0009607; P:response to biotic stimulus; IEA:UniProtKB-KW.
DR   Gene3D; 3.20.20.80; Glycosidases; 1.
DR   InterPro; IPR000490; Glyco_hydro_17.
DR   InterPro; IPR044965; Glyco_hydro_17_plant.
DR   InterPro; IPR017853; Glycoside_hydrolase_SF.
DR   PANTHER; PTHR32227; GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATED; 1.
DR   PANTHER; PTHR32227:SF232; GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG3-RELATED; 1.
DR   Pfam; PF00332; Glyco_hydro_17; 1.
DR   SUPFAM; SSF51445; (Trans)glycosidases; 1.
DR   PROSITE; PS00587; GLYCOSYL_HYDROL_F17; 1.
PE   3: Inferred from homology;
KW   Glycosidase; Hydrolase; Pathogenesis-related protein; Plant defense;
KW   Signal.
FT   SIGNAL          1..31
FT                   /evidence="ECO:0000255"
FT   CHAIN           32..350
FT                   /note="Glucan endo-1,3-beta-glucosidase, basic isoform"
FT                   /id="PRO_0000011859"
FT   ACT_SITE        132
FT                   /note="Proton donor"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
FT   ACT_SITE        274
FT                   /note="Nucleophile"
FT                   /evidence="ECO:0000250|UniProtKB:O22317"
SQ   SEQUENCE   350 AA;  38232 MW;  68C9A1E80DE6FAD9 CRC64;
     MTKSNSSSVG RLLSLISIVL LLGQLVVGSL ATKQHTGAPI GVCNGMVGDD LPPQAEVVAL
     YKTNNIPRMR LYDPNPAALE ALRGSNIKLL LGVPNENLQY IALSQANANA WVQNNVRNYA
     NVKFKYIAVG NEVKPSDSFA QFLVPAMRNI QEAISLAGLA KKIKVSTAID TGVLGETFPP
     SIGSFKSEYN ALLYPIIRFL VSHQSPLLVN LYPYFAYSGN TQDIRLDYAL FTAPSVVVQD
     GNFGYRNLFD AMLDGVYAAL EKAGGGSLKV VISETGWPSA AGTATTIDNA RTFISNLIQH
     VKEGTPRRPG RPIETYIFAM FDENRKTPEL EKHWGLFSPT KQPKYQISFN
//