Reviewed, UniProtKB/Swiss-Prot P52408 (E13B_PRUPE)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase, basic isoform
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase
      Short name=(1->3)-beta-glucanase
    Beta-1,3-endoglucanase
    PpGns1

Gene names

Name:

GNS1

Organism

Prunus persica (Peach)

Taxonomic identifier

3760 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids I › Rosales › Rosaceae › Amygdaloideae › Prunus

Protein attributes

Sequence length	350 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Domain	Signal
Molecular function	Glycosidase Hydrolase Pathogenesis-related protein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW response to biotic stimulus Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 31	31	Potential
	Chain	32 – 350	319	Glucan endo-1,3-beta-glucosidase, basic isoform		PRO_0000011859
Sites
	Active site	274	1	Nucleophile By similarity
	Active site	331	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52408-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 68C9A1E80DE6FAD9	FASTA	350	38,232
	10 20 30 40 50 60 MTKSNSSSVG RLLSLISIVL LLGQLVVGSL ATKQHTGAPI GVCNGMVGDD LPPQAEVVAL 70 80 90 100 110 120 YKTNNIPRMR LYDPNPAALE ALRGSNIKLL LGVPNENLQY IALSQANANA WVQNNVRNYA 130 140 150 160 170 180 NVKFKYIAVG NEVKPSDSFA QFLVPAMRNI QEAISLAGLA KKIKVSTAID TGVLGETFPP 190 200 210 220 230 240 SIGSFKSEYN ALLYPIIRFL VSHQSPLLVN LYPYFAYSGN TQDIRLDYAL FTAPSVVVQD 250 260 270 280 290 300 GNFGYRNLFD AMLDGVYAAL EKAGGGSLKV VISETGWPSA AGTATTIDNA RTFISNLIQH 310 320 330 340 350 VKEGTPRRPG RPIETYIFAM FDENRKTPEL EKHWGLFSPT KQPKYQISFN

References

[1]	"Characterization and expression of beta-1,3-glucanase genes in peach." Thimmapuram J., Ko T.S., Korban S.S. Mol. Genet. Genomics 265:469-479(2001) [PubMed: 11405630] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	U49454 Genomic DNA. Translation: AAA92013.1.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 39734.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13B_PRUPE

Accession

Primary (citable) accession number: P52408

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)