Reviewed, UniProtKB/Swiss-Prot P52402 (E133_SOLTU)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase, basic isoform 3
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase
      Short name=(1->3)-beta-glucanase
    Beta-1,3-endoglucanase

Gene names

Name:

GLUB3

Organism

Solanum tuberosum (Potato)

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	328 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole By similarity.
Developmental stage	Highest levels in old segments of leaves, stems and roots.
Induction	In response to infection, elicitor, ethylene, wounding.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 305	›305	Glucan endo-1,3-beta-glucosidase, basic isoform 3		PRO_0000011864
	Propeptide	306 – 328	23	Removed in mature form By similarity		PRO_0000011865
Sites
	Active site	230	1	Nucleophile By similarity
	Active site	286	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	318	1	N-linked (GlcNAc...) Potential
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P52402-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: F2F9266304B89454	FASTA	328	36,182
	10 20 30 40 50 60 NNLPSHSEVI QLYKSRNIGR LRLYDPNHGA LNALRGSNIE VILGLPNVDV KHIASGMEHA 70 80 90 100 110 120 RWWVQKNVKD FWPDVKIKYI AVGNEISPVT GTSSLTSFQV PALVNIYKAI GEAGLGNDIK 130 140 150 160 170 180 VSTSVDMTLI GNSYPPSQGS FRNDVRWFTD PIVGFLRDTR APLLVNIYPY FSYSGNPGQI 190 200 210 220 230 240 SLPYALFTAP NVVVQDGSRQ YRNLFDAMLD SVYAAMERTG GGSVGIVVSE SGWPSAGAFG 250 260 270 280 290 300 ATQDNAATYL RNLIQHAKEG SPRKPGPIET YIFAMFDENN KNPELEKHFG LFSPNKQPKY 310 320 NLNFGVSERV WDISAETNST TSSLISEM

References

[1]	"Primary structure and expression of mRNAs encoding basic chitinase and 1,3-beta-glucanase in potato." Beerhues L., Kombrink E. Plant Mol. Biol. 24:353-367(1994) [PubMed: 8111037] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Datura. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U01902 mRNA. Translation: AAA19111.1.
PIR	S65023.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 296.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E133_SOLTU

Accession

Primary (citable) accession number: P52402

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 51 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)