P52401 (E132_SOLTU) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase, basic isoform 2
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase

Gene names

Name:

GLUB2

Organism

Solanum tuberosum (Potato) [Reference proteome]

Taxonomic identifier

4113 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Solanoideae › Solaneae › Solanum

Protein attributes

Sequence length	363 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Vacuole By similarity.
Tissue specificity	High levels in leaves. Appreciable amounts in stems, roots, and sepals. Tubers, root tips, and all other flower organs contain very low levels of enzyme.
Developmental stage	Highest levels in old segments of leaves, stems and roots.
Induction	In leaves, in response to infection, elicitor, ethylene, or wounding.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Vacuole
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Pyrrolidone carboxylic acid
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	vacuole Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 340	315	Glucan endo-1,3-beta-glucosidase, basic isoform 2		PRO_0000011862
	Propeptide	341 – 363	23	Removed in mature form By similarity		PRO_0000011863
Sites
	Active site	265	1	Nucleophile By similarity
	Active site	321	1	Proton donor By similarity
Amino acid modifications
	Modified residue	26	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	353	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P52401 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 201A1A6A50C30DCC	FASTA	363	39,759
	10 20 30 40 50 60 MATSQIAVIV LLGLLVATNI HITEAQLGVC YGMMGNNLPS HSEVIQLYKS RNIGRLRLYD 70 80 90 100 110 120 PNQGALNALR GSNIEVILGL PNVDVKHIAS GMEHARWWVQ KNVKDFWPDV KIKYIAVGNE 130 140 150 160 170 180 ISPVTGTSSL TSFQVPALVN IYKAVGEAGL GNDIKVSTSV DMTLIGNSYP PSQGSFRNDV 190 200 210 220 230 240 RWFTDPIVGF LRDTRAPLLV NIYPYFSYSG NPGQISLPYA LFTAPNVVVQ DGSRQYRNLF 250 260 270 280 290 300 DAMLDSVYAA MERTGGGSVG IVVSECGWPS AGAFGATQDN AATYLRNLIQ HAKEGSPRKP 310 320 330 340 350 360 GPIETYIFAM FDENNKNPEL EKHFGLFSPN KQPKYNLNFG VSERVWDISA ETNSTASSLI SEM

References

[1]	"Primary structure and expression of mRNAs encoding basic chitinase and 1,3-beta-glucanase in potato." Beerhues L., Kombrink E. Plant Mol. Biol. 24:353-367(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Datura. Tissue: Leaf.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U01901 mRNA. Translation: AAA18928.1.
PIR	S43318.
3D structure databases
ProteinModelPortal	P52401.
SMR	P52401. Positions 27-339.
ModBase	Search...
Protein family/group databases
Allergome	2551. Sola t Glucanase.
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E132_SOLTU

Accession

Primary (citable) accession number: P52401

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 65 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program