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Protein

Glucan endo-1,3-beta-glucosidase, acidic isoform GL153

Gene

GGL4

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Is thought to be an important plant defense-related product against fungal pathogens.

Catalytic activityi

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei264NucleophileBy similarity1
Active sitei324Proton donorBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Glycosidase, Hydrolase

Keywords - Biological processi

Plant defense

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Names & Taxonomyi

Protein namesi
Recommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform GL153 (EC:3.2.1.39)
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name:
(1->3)-beta-glucanase
Beta-1,3-endoglucanase
Gene namesi
Name:GGL4
OrganismiNicotiana tabacum (Common tobacco)
Taxonomic identifieri4097 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Secreted

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 29Sequence analysisAdd BLAST29
ChainiPRO_000001188030 – 356Glucan endo-1,3-beta-glucosidase, acidic isoform GL153Add BLAST327

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei30Pyrrolidone carboxylic acidCurated1
Glycosylationi95N-linked (GlcNAc...)Sequence analysis1

Keywords - PTMi

Glycoprotein, Pyrrolidone carboxylic acid

Expressioni

Tissue specificityi

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Inductioni

By viral infection.

Structurei

3D structure databases

ProteinModelPortaliP52399.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 17 family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52399-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MALCIKNGFL AAALVLVGLL MCSIQMIGAQ SIGVCYGKIA NNLPSEQDVI
60 70 80 90 100
NLYKANGIRK MRIYYPDKNI FKALKGSNIE IILDVPNQDL EALANSSIAN
110 120 130 140 150
GWVQDNIRSH FPYVKFKYIS IGNEVSPINN GQYSQFLLHA MENVYNALAA
160 170 180 190 200
SGLQDKIKVT TATYSGLLAN TYPPKASIFR GEFNSFINPI IQFLAQNNLP
210 220 230 240 250
LLANVYPYFV HISNTADVPL SYALFTQRGK NSAGYQNLFD AILDSMYFAV
260 270 280 290 300
EKAGGPNVEI IVSESGWPSE GNSAATIENA QTYYRNLIDH VKRGAGTPKK
310 320 330 340 350
PGKSIETYLF AMFDENVKKG EITEKHFGLF SPDQRAKYQL NFNSLMPIYI

DISRVI
Length:356
Mass (Da):39,489
Last modified:October 1, 1996 - v1
Checksum:i640A904884C4A4DE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60463 mRNA. Translation: AAA34079.1.
AF141654 Genomic DNA. Translation: AAD33881.1.
PIRiT03249.
RefSeqiNP_001312401.1. NM_001325472.1.
UniGeneiNta.3488.

Genome annotation databases

GeneIDi107789548.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M60463 mRNA. Translation: AAA34079.1.
AF141654 Genomic DNA. Translation: AAD33881.1.
PIRiT03249.
RefSeqiNP_001312401.1. NM_001325472.1.
UniGeneiNta.3488.

3D structure databases

ProteinModelPortaliP52399.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

CAZyiGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi107789548.

Family and domain databases

Gene3Di3.20.20.80. 1 hit.
InterProiIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamiPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMiSSF51445. SSF51445. 1 hit.
PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiE13L_TOBAC
AccessioniPrimary (citable) accession number: P52399
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 5, 2016
This is version 71 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.