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P52399 (E13L_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform GL153

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase
Gene names
Name:GGL4
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length356 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Is thought to be an important plant defense-related product against fungal pathogens.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Secretedextracellular space.

Tissue specificity

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Induction

By viral infection.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Pyrrolidone carboxylic acid
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucan endo-1,3-beta-D-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2929 Potential
Chain30 – 356327Glucan endo-1,3-beta-glucosidase, acidic isoform GL153
PRO_0000011880

Sites

Active site2641Nucleophile By similarity
Active site3241Proton donor By similarity

Amino acid modifications

Modified residue301Pyrrolidone carboxylic acid Probable
Glycosylation951N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P52399 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 640A904884C4A4DE

FASTA35639,489
        10         20         30         40         50         60 
MALCIKNGFL AAALVLVGLL MCSIQMIGAQ SIGVCYGKIA NNLPSEQDVI NLYKANGIRK 

        70         80         90        100        110        120 
MRIYYPDKNI FKALKGSNIE IILDVPNQDL EALANSSIAN GWVQDNIRSH FPYVKFKYIS 

       130        140        150        160        170        180 
IGNEVSPINN GQYSQFLLHA MENVYNALAA SGLQDKIKVT TATYSGLLAN TYPPKASIFR 

       190        200        210        220        230        240 
GEFNSFINPI IQFLAQNNLP LLANVYPYFV HISNTADVPL SYALFTQRGK NSAGYQNLFD 

       250        260        270        280        290        300 
AILDSMYFAV EKAGGPNVEI IVSESGWPSE GNSAATIENA QTYYRNLIDH VKRGAGTPKK 

       310        320        330        340        350 
PGKSIETYLF AMFDENVKKG EITEKHFGLF SPDQRAKYQL NFNSLMPIYI DISRVI 

« Hide

References

[1]"Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection."
Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A.
Plant Physiol. 96:390-397(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Xanthi NC.
Tissue: Leaf.
[2]"Isolation of a genomic DNA clone (GGL4) encoding a beta-1,3-glucanase from Nicotiana tabacum cv. Xanthi nc."
Chen H.-J., Hou W.-C., Lin Y.-H.
Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
Cited for: NUCLEOTIDE SEQUENCE.
Strain: cv. Xanthi NC.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60463 mRNA. Translation: AAA34079.1.
AF141654 Genomic DNA. Translation: AAD33881.1.
PIRT03249.

3D structure databases

ProteinModelPortalP52399.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE13L_TOBAC
AccessionPrimary (citable) accession number: P52399
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 9, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries