P52399 (E13L_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform GL153
EC=3.2.1.39

Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase

Gene names

Name:

GGL4

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Viridiplantae › Streptophyta › Streptophytina › Embryophyta › Tracheophyta › Euphyllophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	356 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.
Induction	By viral infection.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological_process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 29	29	Potential
	Chain	30 – 356	327	Glucan endo-1,3-beta-glucosidase, acidic isoform GL153		PRO_0000011880
Sites
	Active site	264	1	Nucleophile By similarity
	Active site	324	1	Proton donor By similarity
Amino acid modifications
	Modified residue	30	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	95	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P52399 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 640A904884C4A4DE	FASTA	356	39,489
	10 20 30 40 50 60 MALCIKNGFL AAALVLVGLL MCSIQMIGAQ SIGVCYGKIA NNLPSEQDVI NLYKANGIRK 70 80 90 100 110 120 MRIYYPDKNI FKALKGSNIE IILDVPNQDL EALANSSIAN GWVQDNIRSH FPYVKFKYIS 130 140 150 160 170 180 IGNEVSPINN GQYSQFLLHA MENVYNALAA SGLQDKIKVT TATYSGLLAN TYPPKASIFR 190 200 210 220 230 240 GEFNSFINPI IQFLAQNNLP LLANVYPYFV HISNTADVPL SYALFTQRGK NSAGYQNLFD 250 260 270 280 290 300 AILDSMYFAV EKAGGPNVEI IVSESGWPSE GNSAATIENA QTYYRNLIDH VKRGAGTPKK 310 320 330 340 350 PGKSIETYLF AMFDENVKKG EITEKHFGLF SPDQRAKYQL NFNSLMPIYI DISRVI

References

[1]	"Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection." Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A. Plant Physiol. 96:390-397(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Xanthi NC. Tissue: Leaf.
[2]	"Isolation of a genomic DNA clone (GGL4) encoding a beta-1,3-glucanase from Nicotiana tabacum cv. Xanthi nc." Chen H.-J., Hou W.-C., Lin Y.-H. Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE. Strain: cv. Xanthi NC.

Cross-references

Sequence databases
EMBL GenBank DDBJ	M60463 mRNA. Translation: AAA34079.1. AF141654 Genomic DNA. Translation: AAD33881.1.
PIR	T03249.
3D structure databases
ProteinModelPortal	P52399.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
Gene3D	3.20.20.80. 1 hit.
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_catalytic_dom. IPR017853. Glycoside_hydrolase_SF. [Graphical view]
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
SUPFAM	SSF51445. Glyco_hydro_cat. 1 hit.
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13L_TOBAC

Accession

Primary (citable) accession number: P52399

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 63 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Plant Protein Annotation Program