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P52399

- E13L_TOBAC

UniProt

P52399 - E13L_TOBAC

Protein

Glucan endo-1,3-beta-glucosidase, acidic isoform GL153

Gene

GGL4

Organism
Nicotiana tabacum (Common tobacco)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at transcript leveli
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    • History
      Entry version 67 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Is thought to be an important plant defense-related product against fungal pathogens.

    Catalytic activityi

    Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei264 – 2641NucleophileBy similarity
    Active sitei324 – 3241Proton donorBy similarity

    GO - Molecular functioni

    1. glucan endo-1,3-beta-D-glucosidase activity Source: UniProtKB-EC

    GO - Biological processi

    1. carbohydrate metabolic process Source: InterPro
    2. defense response Source: UniProtKB-KW

    Keywords - Molecular functioni

    Glycosidase, Hydrolase

    Keywords - Biological processi

    Plant defense

    Protein family/group databases

    CAZyiGH17. Glycoside Hydrolase Family 17.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Glucan endo-1,3-beta-glucosidase, acidic isoform GL153 (EC:3.2.1.39)
    Alternative name(s):
    (1->3)-beta-glucan endohydrolase
    Short name:
    (1->3)-beta-glucanase
    Beta-1,3-endoglucanase
    Gene namesi
    Name:GGL4
    OrganismiNicotiana tabacum (Common tobacco)
    Taxonomic identifieri4097 [NCBI]
    Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

    Subcellular locationi

    GO - Cellular componenti

    1. extracellular space Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Secreted

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Signal peptidei1 – 2929Sequence AnalysisAdd
    BLAST
    Chaini30 – 356327Glucan endo-1,3-beta-glucosidase, acidic isoform GL153PRO_0000011880Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei30 – 301Pyrrolidone carboxylic acidCurated
    Glycosylationi95 – 951N-linked (GlcNAc...)Sequence Analysis

    Keywords - PTMi

    Glycoprotein, Pyrrolidone carboxylic acid

    Expressioni

    Tissue specificityi

    Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

    Inductioni

    By viral infection.

    Structurei

    3D structure databases

    ProteinModelPortaliP52399.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the glycosyl hydrolase 17 family.Curated

    Keywords - Domaini

    Signal

    Family and domain databases

    Gene3Di3.20.20.80. 1 hit.
    InterProiIPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view]
    PfamiPF00332. Glyco_hydro_17. 1 hit.
    [Graphical view]
    SUPFAMiSSF51445. SSF51445. 1 hit.
    PROSITEiPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Sequence processingi: The displayed sequence is further processed into a mature form.

    P52399-1 [UniParc]FASTAAdd to Basket

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    MALCIKNGFL AAALVLVGLL MCSIQMIGAQ SIGVCYGKIA NNLPSEQDVI    50
    NLYKANGIRK MRIYYPDKNI FKALKGSNIE IILDVPNQDL EALANSSIAN 100
    GWVQDNIRSH FPYVKFKYIS IGNEVSPINN GQYSQFLLHA MENVYNALAA 150
    SGLQDKIKVT TATYSGLLAN TYPPKASIFR GEFNSFINPI IQFLAQNNLP 200
    LLANVYPYFV HISNTADVPL SYALFTQRGK NSAGYQNLFD AILDSMYFAV 250
    EKAGGPNVEI IVSESGWPSE GNSAATIENA QTYYRNLIDH VKRGAGTPKK 300
    PGKSIETYLF AMFDENVKKG EITEKHFGLF SPDQRAKYQL NFNSLMPIYI 350
    DISRVI 356
    Length:356
    Mass (Da):39,489
    Last modified:October 1, 1996 - v1
    Checksum:i640A904884C4A4DE
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60463 mRNA. Translation: AAA34079.1.
    AF141654 Genomic DNA. Translation: AAD33881.1.
    PIRiT03249.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    M60463 mRNA. Translation: AAA34079.1 .
    AF141654 Genomic DNA. Translation: AAD33881.1 .
    PIRi T03249.

    3D structure databases

    ProteinModelPortali P52399.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    CAZyi GH17. Glycoside Hydrolase Family 17.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    Gene3Di 3.20.20.80. 1 hit.
    InterProi IPR000490. Glyco_hydro_17.
    IPR013781. Glyco_hydro_catalytic_dom.
    IPR017853. Glycoside_hydrolase_SF.
    [Graphical view ]
    Pfami PF00332. Glyco_hydro_17. 1 hit.
    [Graphical view ]
    SUPFAMi SSF51445. SSF51445. 1 hit.
    PROSITEi PS00587. GLYCOSYL_HYDROL_F17. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection."
      Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A.
      Plant Physiol. 96:390-397(1991) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Xanthi NC.
      Tissue: Leaf.
    2. "Isolation of a genomic DNA clone (GGL4) encoding a beta-1,3-glucanase from Nicotiana tabacum cv. Xanthi nc."
      Chen H.-J., Hou W.-C., Lin Y.-H.
      Submitted (APR-1999) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE.
      Strain: cv. Xanthi NC.

    Entry informationi

    Entry nameiE13L_TOBAC
    AccessioniPrimary (citable) accession number: P52399
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 67 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programPlant Protein Annotation Program

    Miscellaneousi

    Documents

    1. Glycosyl hydrolases
      Classification of glycosyl hydrolase families and list of entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3