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P52398 (E13K_TOBAC) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Glucan endo-1,3-beta-glucosidase, acidic isoform GL161

EC=3.2.1.39
Alternative name(s):
(1->3)-beta-glucan endohydrolase
Short name=(1->3)-beta-glucanase
Beta-1,3-endoglucanase
OrganismNicotiana tabacum (Common tobacco)
Taxonomic identifier4097 [NCBI]
Taxonomic lineageEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaeasteridslamiidsSolanalesSolanaceaeNicotianoideaeNicotianeaeNicotiana

Protein attributes

Sequence length331 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Is thought to be an important plant defense-related product against fungal pathogens.

Catalytic activity

Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.

Subcellular location

Secretedextracellular space.

Tissue specificity

Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.

Induction

By viral infection.

Sequence similarities

Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
   Biological processPlant defense
   Cellular componentSecreted
   DomainSignal
   Molecular functionGlycosidase
Hydrolase
   PTMGlycoprotein
Pyrrolidone carboxylic acid
Gene Ontology (GO)
   Biological_processcarbohydrate metabolic process

Inferred from electronic annotation. Source: InterPro

defense response

Inferred from electronic annotation. Source: UniProtKB-KW

   Cellular_componentextracellular space

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionglucan endo-1,3-beta-D-glucosidase activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 2626 Potential
Chain27 – 331305Glucan endo-1,3-beta-glucosidase, acidic isoform GL161
PRO_0000011879

Sites

Active site2441Nucleophile By similarity
Active site3041Proton donor By similarity

Amino acid modifications

Modified residue271Pyrrolidone carboxylic acid Probable
Glycosylation551N-linked (GlcNAc...) Potential
Glycosylation751N-linked (GlcNAc...) Potential

Sequences

Sequence LengthMass (Da)Tools
P52398 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 5F0171DE53451BBA

FASTA33136,891
        10         20         30         40         50         60 
MCSIQIIGAQ SIGVCYGKAA NNLPSDQDVI NLYNANGIRK LRIYYPDKNI FKALNGSNIE 

        70         80         90        100        110        120 
IILGVPNQDL EALANSSIAN GWVQDNIRSH FPYVKFKYIS IGNKVSPTNN DQYSEFLLQA 

       130        140        150        160        170        180 
MKNVYNALAA AGLQDMIKVS TVTYSGVLAN TYPPERSIFR EEFKSFINPI IQFLARNNLP 

       190        200        210        220        230        240 
LLANVYPYFV HVSNTADVSL SYALFTQQGT NSAGYQNLFD AILDSMYFAV EKAGGPNVEI 

       250        260        270        280        290        300 
IVSESGWPSE GSSAATIENA QTYYRNLINH VKSGAGTPKK PGKTIETYLF AMFDENDKIG 

       310        320        330 
EITEKHFGLF SPDQRAKYQL NFNYLPIYIL R 

« Hide

References

[1]"Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection."
Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A.
Plant Physiol. 96:390-397(1991) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
Strain: cv. Xanthi NC.
Tissue: Leaf.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
M60464 mRNA. Translation: AAA34053.1.
PIRT02343.

3D structure databases

ProteinModelPortalP52398.
ModBaseSearch...
MobiDBSearch...

Protein family/group databases

CAZyGH17. Glycoside Hydrolase Family 17.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

Gene3D3.20.20.80. 1 hit.
InterProIPR000490. Glyco_hydro_17.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
[Graphical view]
PfamPF00332. Glyco_hydro_17. 1 hit.
[Graphical view]
SUPFAMSSF51445. SSF51445. 1 hit.
PROSITEPS00587. GLYCOSYL_HYDROL_F17. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameE13K_TOBAC
AccessionPrimary (citable) accession number: P52398
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 19, 2014
This is version 66 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Glycosyl hydrolases

Classification of glycosyl hydrolase families and list of entries