Reviewed, UniProtKB/Swiss-Prot P52398 (E13K_TOBAC)

Names and origin

Protein names	Recommended name:     Glucan endo-1,3-beta-glucosidase, acidic isoform GL161     EC=3.2.1.39 Alternative name(s):     (1->3)-beta-glucan endohydrolase       Short name=(1->3)-beta-glucanase     Beta-1,3-endoglucanase
Organism	Nicotiana tabacum (Common tobacco)
Taxonomic identifier	4097 [NCBI]
Taxonomic lineage	Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	331 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Is thought to be an important plant defense-related product against fungal pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space.
Tissue specificity	Is expressed primarily in epidermal cell of healthy plant, and following induction by ethylene, accumulates in mesophyll cells.
Induction	By viral infection.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Domain	Signal
Molecular function	Glycosidase Hydrolase
PTM	Glycoprotein Pyrrolidone carboxylic acid
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 26	26	Potential
	Chain	27 – 331	305	Glucan endo-1,3-beta-glucosidase, acidic isoform GL161		PRO_0000011879
Sites
	Active site	244	1	Nucleophile By similarity
	Active site	304	1	Proton donor By similarity
Amino acid modifications
	Modified residue	27	1	Pyrrolidone carboxylic acid Probable
	Glycosylation	55	1	N-linked (GlcNAc...) Potential
	Glycosylation	75	1	N-linked (GlcNAc...) Potential

Sequences

	Sequence		Length	Mass (Da)
	P52398-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 5F0171DE53451BBA	FASTA	331	36,891
	10 20 30 40 50 60 MCSIQIIGAQ SIGVCYGKAA NNLPSDQDVI NLYNANGIRK LRIYYPDKNI FKALNGSNIE 70 80 90 100 110 120 IILGVPNQDL EALANSSIAN GWVQDNIRSH FPYVKFKYIS IGNKVSPTNN DQYSEFLLQA 130 140 150 160 170 180 MKNVYNALAA AGLQDMIKVS TVTYSGVLAN TYPPERSIFR EEFKSFINPI IQFLARNNLP 190 200 210 220 230 240 LLANVYPYFV HVSNTADVSL SYALFTQQGT NSAGYQNLFD AILDSMYFAV EKAGGPNVEI 250 260 270 280 290 300 IVSESGWPSE GSSAATIENA QTYYRNLINH VKSGAGTPKK PGKTIETYLF AMFDENDKIG 310 320 330 EITEKHFGLF SPDQRAKYQL NFNYLPIYIL R

References

[1]

"Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection." Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A. Plant Physiol. 96:390-397(1991) [PubMed: 16668198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA]. Strain: cv. Xanthi NC. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60464 mRNA. Translation: AAA34053.1.
PIR	T02343.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 298.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13K_TOBAC

Accession

Primary (citable) accession number: P52398

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 52 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)