Reviewed, UniProtKB/Swiss-Prot P52396 (E13I_TOBAC)

Names and origin

Protein names

Recommended name:
    Glucan endo-1,3-beta-glucosidase, acidic isoform PR-N
    EC=3.2.1.39
Alternative name(s):
    (1->3)-beta-glucan endohydrolase
      Short name=(1->3)-beta-glucanase
    Beta-1,3-endoglucanase

Gene names

Name:

PRN

Organism

Nicotiana tabacum (Common tobacco)

Taxonomic identifier

4097 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › asterids › lamiids › Solanales › Solanaceae › Nicotianoideae › Nicotianeae › Nicotiana

Protein attributes

Sequence length	275 AA.
Sequence status	Fragment.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Implicated in the defense of plants against pathogens.
Catalytic activity	Hydrolysis of (1->3)-beta-D-glucosidic linkages in (1->3)-beta-D-glucans.
Subcellular location	Secreted › extracellular space.
Induction	Not found in healthy tissues, but accumulates to high levels in the extracellular compartment of leaves in response to pathogen infection or treatment with salicylic acid.
Post-translational modification	The N-terminus is blocked.
Sequence similarities	Belongs to the glycosyl hydrolase 17 family.

Ontologies

Keywords
Biological process	Plant defense
Cellular component	Secreted
Molecular function	Glycosidase Hydrolase
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	carbohydrate metabolic process Inferred from electronic annotation. Source: InterPro defense response Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	extracellular space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	cation binding Inferred from electronic annotation. Source: InterPro glucan endo-1,3-beta-D-glucosidase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	‹1 – 275	›275	Glucan endo-1,3-beta-glucosidase, acidic isoform PR-N		PRO_0000205277
Sites
	Active site	196	1	Nucleophile By similarity
	Active site	256	1	Proton donor By similarity
Experimental info
	Non-terminal residue	1	1

Sequences

	Sequence		Length	Mass (Da)
	P52396-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: EFD9B913A50C8FB5	FASTA	275	30,386
	10 20 30 40 50 60 NVFNALRGSN IEIILDVPLQ DLQSLTDPSR ANGWVQDNII NHFPDVKFKY IAVGNKVSPG 70 80 90 100 110 120 NNGQYAPFVA PAMQNVYNAL AAAGLQDQIK VSTATYSGIL ANTYPPKDSI FRGEFNSFIN 130 140 150 160 170 180 PIIQFLVQHN LPLLANVYPY FGHIFNTADV PLSYALFTQQ EANPAGYQNL FDALLDSMYF 190 200 210 220 230 240 AVEKAGGQNV EIIVSESGWP SEGNSAATIE NAQTYYENLI NHVKSGAGTP KKPGKAIETY 250 260 270 LFAMFDENNK EGDITEKHFG LFSPDQRAKY QLNFN

References

[1]

"Differential regulation of beta-1,3-glucanase messenger RNAs in response to pathogen infection." Ward E.R., Payne G.B., Moyer M.B., Williams S.C., Dincher S.S., Sharkey K.C., Beck J.J., Taylor H.T., Ahl-Goy P., Meins F., Ryals J.A. Plant Physiol. 96:390-397(1991) [PubMed: 16668198] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE. Strain: cv. Xanthi NC. Tissue: Leaf.

Cross-references

Sequence databases
EMBLGenBankDDBJ	M60462 mRNA. Translation: AAA34105.1.
3D structure databases
HSSP	HSSP built from PDB template 1GHS based on UniProtKB P15737.
ModBase	Search...
Protein family/group databases
CAZy	GH17. Glycoside Hydrolase Family 17.
Enzyme and pathway databases
BRENDA	3.2.1.39. 298.
Family and domain databases
InterPro	IPR000490. Glyco_hydro_17. IPR013781. Glyco_hydro_sg_catalytic. [Graphical view]
Gene3D	G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit.
Pfam	PF00332. Glyco_hydro_17. 1 hit. [Graphical view]
PROSITE	PS00587. GLYCOSYL_HYDROL_F17. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

E13I_TOBAC

Accession

Primary (citable) accession number: P52396

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)