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Protein

Large tegument protein deneddylase

Gene

U31

Organism
Human herpesvirus 7 (strain JI) (HHV-7) (Human T lymphotropic virus)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Large tegument protein that plays multiple roles in the viral cycle. During viral entry, remains associated with the capsid while most of the tegument is detached and participates in the capsid transport toward the host nucleus. Plays a role in the routing of the capsid at the nuclear pore complex and subsequent uncoating. Within the host nucleus, acts as a deneddylase and promotes the degradation of nuclear CRLs (cullin-RING ubiquitin ligases) and thereby stabilizes nuclear CRL substrates, while cytoplasmic CRLs remain unaffected. These modifications prevent host cell cycle S-phase progression and create a favorable environment allowing efficient viral genome replication. Participates later in the secondary envelopment of capsids. Indeed, plays a linker role for the association of the outer viral tegument to the capsids together with the inner tegument protein.By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei10Important for catalytic activityBy similarity1
Active sitei23By similarity1
Active sitei155By similarity1
Active sitei157By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Large tegument protein deneddylase (EC:3.4.19.12By similarity, EC:3.4.22.-By similarity)
Gene namesi
Name:U31
OrganismiHuman herpesvirus 7 (strain JI) (HHV-7) (Human T lymphotropic virus)
Taxonomic identifieri57278 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeRoseolovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
Proteomesi
  • UP000009246 Componenti: Genome

Subcellular locationi

  • Virion tegument By similarity
  • Host cytoplasm By similarity
  • Host nucleus By similarity

  • Note: Tightly associated with the capsid.By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001160421 – 2059Large tegument protein deneddylaseAdd BLAST2059

Proteomic databases

PRIDEiP52362.

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins. Interacts with inner tegument protein. Interacts with capsid vertex specific component CVC2. Interacts with the major capsid protein/MCP.By similarity

Structurei

3D structure databases

ProteinModelPortaliP52362.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini3 – 220Peptidase C76By similarityAdd BLAST218

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni1 – 230Deubiquitination activityBy similarityAdd BLAST230
Regioni278Interaction with inner tegument proteinBy similarity1

Sequence similaritiesi

Belongs to the herpesviridae large tegument protein family.By similarity
Contains 1 peptidase C76 domain.By similarity

Keywords - Domaini

Repeat

Family and domain databases

InterProiIPR006928. htUSP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52362-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRIIAGSTNQ NDPKYGPRAG KQCMSNCFSF LHTVYLNGIN NVLNKESIDI
60 70 80 90 100
IMENGALLDN ISTTTLKLET GNIPEYRFFT EIPKKISSNF GETIHELSRP
110 120 130 140 150
FNGTLESQHI DNEVYLGLLD FLLYGKNKKP AFIVITIGVM ARAIFIVDEL
160 170 180 190 200
FYLFDSHASD TENSAAIYIC EDIDELYALL AIENVAEFYY DAVFSYFIET
210 220 230 240 250
TDLSLEDGDA TILILKTYKD PDIALSLNDF LTMYSSTSST KTAETNTLIS
260 270 280 290 300
KQSPSKRKQE KTSLNSNSLE KKRKQGSSLK YYNNEVDLVP SFYELRPQFN
310 320 330 340 350
NILFELSNFP IVKENVNWTL YIQKFATKST QPFTKPFIWN RVFHLFSQVV
360 370 380 390 400
DALIMIKNDH WDETQQQKQF FTHFLPFKEF SEEFENAIEA CRENNLDLIL
410 420 430 440 450
LYKNYLSKTT AFKNLERILL TKFSAIVSPV HEKHYTLVNT WLTNLIQKLV
460 470 480 490 500
KHPEDTNAFI NDYVLKNPLN HFICLNKKEK QSIALLLNKK RMSMLKDVEI
510 520 530 540 550
EKNGFVQLQA FIENIGEAPA NYLDPENARK VNVEEVSEKD IPTLSTDKVS
560 570 580 590 600
IPNESMFTSN KKHSIEKLIH AKLKAILSTM GQRLTRIIQE NYNNIAAGFL
610 620 630 640 650
PVNDLNNLFA YLVKLYFDVY SITINGFVVE NELIKNIEQI YDNTQYLRFG
660 670 680 690 700
LTRFNMQNLT PFTISVRKMF LDFFLSQKTL IDRAEEIIEN LEFKSVTPEG
710 720 730 740 750
KQKLATKNML REQLEQLNAM DVDDTINLKT DTLTHQVLFS DQELRMIQDF
760 770 780 790 800
ILQLSIHNIP SINFVKSLKL HIILEKRPDI LLALQEKVQN ILYFYFQDLV
810 820 830 840 850
NEIPAQENVL STMLFIIELF PADSRIHLLE TGYISRHIVK KWLNMKSLQD
860 870 880 890 900
AEDLIRFINI NKEQLGKFEH QPFGKEIQKL IEKIHLFYKQ KVIEYQEDVW
910 920 930 940 950
SEMAKNIILT SPSELSQFLA SAPTQRIIQK HKNNLDQKLL IHMENQAKQA
960 970 980 990 1000
MEDDKKRVAC SKINLERHLN DLLLLLKDRQ FASIQASVLI VCENIFKTIP
1010 1020 1030 1040 1050
DDNLIIQFSH ALLSVLLDIE KDLKSYSSEI LEKILINRPL ETSRLLVFKD
1060 1070 1080 1090 1100
AYGNLKEFLN ALKQSLFATA DVQNKADFLI QILDFTYKFR HKTNKGKLLH
1110 1120 1130 1140 1150
SIYNEDFKLY EETLTELRKK ATDAKESLTK LFKASEQKIE LSRTIPLKEI
1160 1170 1180 1190 1200
YLNIETVNFQ GYGNVVFRES AFKRAIEVEI KNYEMKLNDL IKHFNSHLKT
1210 1220 1230 1240 1250
KIDHIQILNL SFDNKWKDFV SKSKISFPPE LTISSQELIK DPIKVITETL
1260 1270 1280 1290 1300
NKASNDLAYV ISEKILKWLI VFVKELNTFF VATMSEFGEV IPFDYKHFRA
1310 1320 1330 1340 1350
LEYEINSKYI EIENKIICNE IIENTDNIEK LSTLIKQIDP NRIAGGKQKF
1360 1370 1380 1390 1400
QDYLSKILTA ETNQQQTRYK EQLKKQYFDL LDNIAHFRFA FDFNHQQNLI
1410 1420 1430 1440 1450
LKLKDKFKTL RTDTVFERFP NLDDTFVSSM NVENFLQALE ALSHFVQAAQ
1460 1470 1480 1490 1500
NFLQNVLTEQ ADLFPQTNFI PVELSTVKTI PKSDINLRMK IHTPQTFFQV
1510 1520 1530 1540 1550
DSVFNTQLIV DEKGIPVQFY NVFHNIVFKF FALNYKKIIV PDKVLNLVST
1560 1570 1580 1590 1600
KYKILTTLKS ILSVVKSFWK EIINFDLTSY FQGKAEFTFQ NVFPIINLKI
1610 1620 1630 1640 1650
FIYIITQAWS VTSDETQHSF ELPLEKFSLL IIANNPEFLF GSLQCPVDLA
1660 1670 1680 1690 1700
INSLIPLLEK KKYFTAFTIS DNPPKLSMDE LKIVCLDLNT WSEITLEKYT
1710 1720 1730 1740 1750
FKKNSLMQLC MGKEKFFIYL LSALVLPQNF LNYIWIQYKP SCCAQDSFQQ
1760 1770 1780 1790 1800
LIQDLCFEYT HQNHIKPISL NLQEPNALKH GERILSKFVL EKNANTSLFS
1810 1820 1830 1840 1850
IFLGKQFLLD YLLFSYLTAT EMTFSYYVDS IKNFLLTIRH LENVQQNVDF
1860 1870 1880 1890 1900
RTILQSRNFD LKYLLTQSWT QNVLEQSIFH VQLDKIIADI KQPQLSLKKI
1910 1920 1930 1940 1950
PLVLFNGDNE VVSTYVPPEQ ASQTEQSFRI KNIFPNPVQE YSSKNVILFT
1960 1970 1980 1990 2000
NYPKNTKFLF NSPPPKTAAK SYKLPDTTDD INTETLSSPT IQRIPIKGLV
2010 2020 2030 2040 2050
PKENEIVFLP EKNTAHTDSK ETKTHLIDTF NILSQTKGEI KTFSTDFDQT

ISKLKHLYF
Length:2,059
Mass (Da):239,477
Last modified:October 1, 1996 - v1
Checksum:iAAE6CA6DEDC5D316
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43400 Genomic DNA. Translation: AAC54693.1.
PIRiT41933.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43400 Genomic DNA. Translation: AAC54693.1.
PIRiT41933.

3D structure databases

ProteinModelPortaliP52362.
ModBaseiSearch...
MobiDBiSearch...

Proteomic databases

PRIDEiP52362.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

InterProiIPR006928. htUSP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiLTP_HHV7J
AccessioniPrimary (citable) accession number: P52362
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 30, 2016
This is version 61 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.