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P52362 (DEN_HHV7J) Reviewed, UniProtKB/Swiss-Prot

Last modified July 24, 2013. Version 49. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Alternative name(s):
Ubiquitin thioesterase
Gene names
Name:U31
OrganismHuman herpesvirus 7 (strain JI) (HHV-7) (Human T lymphotropic virus) [Complete proteome]
Taxonomic identifier57278 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeRoseolovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length2059 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 14 LRR (leucine-rich) repeats.

Contains 1 peptidase C76 domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20592059Deneddylase
PRO_0000116042

Regions

Domain3 – 220218Peptidase C76
Repeat273 – 29725LRR 1
Repeat536 – 55924LRR 2
Repeat714 – 74128LRR 3
Repeat747 – 77024LRR 4
Repeat852 – 87524LRR 5
Repeat969 – 99123LRR 6
Repeat1525 – 154723LRR 7
Repeat1624 – 164017LRR 8
Repeat1641 – 166525LRR 9
Repeat1678 – 170124LRR 10
Repeat1718 – 174124LRR 11
Repeat1828 – 185225LRR 12
Repeat1978 – 200225LRR 13
Repeat2027 – 204923LRR 14
Coiled coil1103 – 114038 Potential

Sites

Active site231 By similarity
Active site1551 By similarity
Active site1571 By similarity
Site101Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P52362 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: AAE6CA6DEDC5D316

FASTA2,059239,477
        10         20         30         40         50         60 
MRIIAGSTNQ NDPKYGPRAG KQCMSNCFSF LHTVYLNGIN NVLNKESIDI IMENGALLDN 

        70         80         90        100        110        120 
ISTTTLKLET GNIPEYRFFT EIPKKISSNF GETIHELSRP FNGTLESQHI DNEVYLGLLD 

       130        140        150        160        170        180 
FLLYGKNKKP AFIVITIGVM ARAIFIVDEL FYLFDSHASD TENSAAIYIC EDIDELYALL 

       190        200        210        220        230        240 
AIENVAEFYY DAVFSYFIET TDLSLEDGDA TILILKTYKD PDIALSLNDF LTMYSSTSST 

       250        260        270        280        290        300 
KTAETNTLIS KQSPSKRKQE KTSLNSNSLE KKRKQGSSLK YYNNEVDLVP SFYELRPQFN 

       310        320        330        340        350        360 
NILFELSNFP IVKENVNWTL YIQKFATKST QPFTKPFIWN RVFHLFSQVV DALIMIKNDH 

       370        380        390        400        410        420 
WDETQQQKQF FTHFLPFKEF SEEFENAIEA CRENNLDLIL LYKNYLSKTT AFKNLERILL 

       430        440        450        460        470        480 
TKFSAIVSPV HEKHYTLVNT WLTNLIQKLV KHPEDTNAFI NDYVLKNPLN HFICLNKKEK 

       490        500        510        520        530        540 
QSIALLLNKK RMSMLKDVEI EKNGFVQLQA FIENIGEAPA NYLDPENARK VNVEEVSEKD 

       550        560        570        580        590        600 
IPTLSTDKVS IPNESMFTSN KKHSIEKLIH AKLKAILSTM GQRLTRIIQE NYNNIAAGFL 

       610        620        630        640        650        660 
PVNDLNNLFA YLVKLYFDVY SITINGFVVE NELIKNIEQI YDNTQYLRFG LTRFNMQNLT 

       670        680        690        700        710        720 
PFTISVRKMF LDFFLSQKTL IDRAEEIIEN LEFKSVTPEG KQKLATKNML REQLEQLNAM 

       730        740        750        760        770        780 
DVDDTINLKT DTLTHQVLFS DQELRMIQDF ILQLSIHNIP SINFVKSLKL HIILEKRPDI 

       790        800        810        820        830        840 
LLALQEKVQN ILYFYFQDLV NEIPAQENVL STMLFIIELF PADSRIHLLE TGYISRHIVK 

       850        860        870        880        890        900 
KWLNMKSLQD AEDLIRFINI NKEQLGKFEH QPFGKEIQKL IEKIHLFYKQ KVIEYQEDVW 

       910        920        930        940        950        960 
SEMAKNIILT SPSELSQFLA SAPTQRIIQK HKNNLDQKLL IHMENQAKQA MEDDKKRVAC 

       970        980        990       1000       1010       1020 
SKINLERHLN DLLLLLKDRQ FASIQASVLI VCENIFKTIP DDNLIIQFSH ALLSVLLDIE 

      1030       1040       1050       1060       1070       1080 
KDLKSYSSEI LEKILINRPL ETSRLLVFKD AYGNLKEFLN ALKQSLFATA DVQNKADFLI 

      1090       1100       1110       1120       1130       1140 
QILDFTYKFR HKTNKGKLLH SIYNEDFKLY EETLTELRKK ATDAKESLTK LFKASEQKIE 

      1150       1160       1170       1180       1190       1200 
LSRTIPLKEI YLNIETVNFQ GYGNVVFRES AFKRAIEVEI KNYEMKLNDL IKHFNSHLKT 

      1210       1220       1230       1240       1250       1260 
KIDHIQILNL SFDNKWKDFV SKSKISFPPE LTISSQELIK DPIKVITETL NKASNDLAYV 

      1270       1280       1290       1300       1310       1320 
ISEKILKWLI VFVKELNTFF VATMSEFGEV IPFDYKHFRA LEYEINSKYI EIENKIICNE 

      1330       1340       1350       1360       1370       1380 
IIENTDNIEK LSTLIKQIDP NRIAGGKQKF QDYLSKILTA ETNQQQTRYK EQLKKQYFDL 

      1390       1400       1410       1420       1430       1440 
LDNIAHFRFA FDFNHQQNLI LKLKDKFKTL RTDTVFERFP NLDDTFVSSM NVENFLQALE 

      1450       1460       1470       1480       1490       1500 
ALSHFVQAAQ NFLQNVLTEQ ADLFPQTNFI PVELSTVKTI PKSDINLRMK IHTPQTFFQV 

      1510       1520       1530       1540       1550       1560 
DSVFNTQLIV DEKGIPVQFY NVFHNIVFKF FALNYKKIIV PDKVLNLVST KYKILTTLKS 

      1570       1580       1590       1600       1610       1620 
ILSVVKSFWK EIINFDLTSY FQGKAEFTFQ NVFPIINLKI FIYIITQAWS VTSDETQHSF 

      1630       1640       1650       1660       1670       1680 
ELPLEKFSLL IIANNPEFLF GSLQCPVDLA INSLIPLLEK KKYFTAFTIS DNPPKLSMDE 

      1690       1700       1710       1720       1730       1740 
LKIVCLDLNT WSEITLEKYT FKKNSLMQLC MGKEKFFIYL LSALVLPQNF LNYIWIQYKP 

      1750       1760       1770       1780       1790       1800 
SCCAQDSFQQ LIQDLCFEYT HQNHIKPISL NLQEPNALKH GERILSKFVL EKNANTSLFS 

      1810       1820       1830       1840       1850       1860 
IFLGKQFLLD YLLFSYLTAT EMTFSYYVDS IKNFLLTIRH LENVQQNVDF RTILQSRNFD 

      1870       1880       1890       1900       1910       1920 
LKYLLTQSWT QNVLEQSIFH VQLDKIIADI KQPQLSLKKI PLVLFNGDNE VVSTYVPPEQ 

      1930       1940       1950       1960       1970       1980 
ASQTEQSFRI KNIFPNPVQE YSSKNVILFT NYPKNTKFLF NSPPPKTAAK SYKLPDTTDD 

      1990       2000       2010       2020       2030       2040 
INTETLSSPT IQRIPIKGLV PKENEIVFLP EKNTAHTDSK ETKTHLIDTF NILSQTKGEI 

      2050 
KTFSTDFDQT ISKLKHLYF 

« Hide

References

[1]"Determination and analysis of the complete nucleotide sequence of human herpesvirus."
Nicholas J.
J. Virol. 70:5975-5989(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U43400 Genomic DNA. Translation: AAC54693.1.
PIRT41933.

3D structure databases

ProteinModelPortalP52362.
ModBaseSearch...
MobiDBSearch...

Proteomic databases

PRIDEP52362.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_HHV7J
AccessionPrimary (citable) accession number: P52362
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: July 24, 2013
This is version 49 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries