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P52340 (DEN_HHV6U) Reviewed, UniProtKB/Swiss-Prot

Last modified June 26, 2013. Version 54. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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Names and origin

Protein namesRecommended name:
Deneddylase

EC=3.4.19.12
EC=3.4.22.-
Gene names
Name:U31
Synonyms:HHRF1
OrganismHuman herpesvirus 6A (strain Uganda-1102) (HHV-6 variant A) (Human B lymphotropic virus) [Reference proteome]
Taxonomic identifier10370 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeRoseolovirus
Virus hostHomo sapiens (Human) [TaxID: 9606]

Protein attributes

Sequence length2077 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activity

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Subunit structure

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Subcellular location

Virion tegument. Host cytoplasm. Host nucleus By similarity.

Sequence similarities

Belongs to the herpesviridae deneddylase family.

Contains 1 peptidase C76 domain.

Sequence caution

The sequence AAA16739.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 20772077Deneddylase
PRO_0000116041

Regions

Domain3 – 221219Peptidase C76
Coiled coil496 – 52429 Potential
Coiled coil1114 – 115441 Potential
Coiled coil1185 – 121430 Potential
Compositional bias280 – 2834Poly-Lys
Compositional bias375 – 3784Poly-Gln

Sites

Active site231 By similarity
Active site1561 By similarity
Active site1581 By similarity
Site101Important for catalytic activity By similarity

Sequences

Sequence LengthMass (Da)Tools
P52340 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: C1CA4BDC26650511

FASTA2,077239,947
        10         20         30         40         50         60 
MKIITSSTNQ NDSKYGPRAG KQCMSNSFSF LHTVYLNGIN NSLNAGTIDA IMEEGYHLDT 

        70         80         90        100        110        120 
AGTLALMLNN SDSQDYRLPT EIPKRIHSRY GVTQHELSRP FNGTLDTQKI DNEVYLGLID 

       130        140        150        160        170        180 
FILYGKSKNG PTFAVITIGV LSRALFFLNN TLYLFDSHPT EREATAAIYI CQNIEEVYEL 

       190        200        210        220        230        240 
LTTHGTEGFY YDASLIFFIE TSNLSLSSHD AELLILKTYK DPDIAIALDK FSSTEIHEIK 

       250        260        270        280        290        300 
KTDDIGSQQD LVADKTTDLE HAPHKRKKNS HSLELELNDK KKKDTASLTY YATEVDLIPS 

       310        320        330        340        350        360 
FYELRSQFQS LFHDLKSFPI MKSNFNWTIY LQDSPINPNQ PFATPFLWNR VFHLLCQIID 

       370        380        390        400        410        420 
VFVGVGSTND DSSKQQQQTI FINYLLPFKD FSEVFNEALA ACQENNLDII FIYNNYLCKT 

       430        440        450        460        470        480 
TTFRTLERIL LSKFLAIVDN DHKKHYEWVK SWTTQMFQGM PKKLDDIENY LKAYVDHNPV 

       490        500        510        520        530        540 
KHFHEFICLN KAEKYKVAVL LNEKRKEIQE AIEREKNSFA QLSNFIDKLG ETPALPIESE 

       550        560        570        580        590        600 
NVHKVHTSDI TEGIVPRFIT ESIELPNIST LNNTQQISLD KQLNEKLTNT IHTLTNKFTK 

       610        620        630        640        650        660 
IVQDNYNNIA AGFMPVTELN CLFAYLVNLY FNIEVLKHSG LNINTVLLQE VEKLYDNTQF 

       670        680        690        700        710        720 
LRFGTSHFNI NNLSNFTLSI RKMFVDFYNS QKPSDRASEI LAAIESILAD PSKNKTVVNI 

       730        740        750        760        770        780 
EMIKSQLEEL GKMEISTTEN KQTAAITKQI LGDQELTPIY DFLHHLSAYN LPNTTTVKNL 

       790        800        810        820        830        840 
HLHFILEQRP DIAMTLHDKI QSILDIYVDD MLNDITVPEQ TFSTVLFLVD LFPNSTEKTA 

       850        860        870        880        890        900 
LFESVLTLRQ LAKKCANLKT LDEFDDLAQF ITTNSEQLQN MMRQHFGKKI PTLMGHIKFL 

       910        920        930        940        950        960 
YSQKIITTEE KNWIQRAKTV VITSPEELTA FLATAPTKHA LQTCKPDLDK ALQRHMEEQM 

       970        980        990       1000       1010       1020 
KQTAENDKKH ILTIRSTLEK RLTDILLILK DGQFSSLETM HLNLLETFLK QLQDNNVIIH 

      1030       1040       1050       1060       1070       1080 
FTHALLPVLK DIETTISKII SDVIEKILIK TPLNPEQMSK EEQKYTPLLS FLSKFKKTTF 

      1090       1100       1110       1120       1130       1140 
CTEDVKTEIE QVQKSITFLK KIATSTNKHT RLSHSIYGQE LNLYEERITE LRKETNKMKE 

      1150       1160       1170       1180       1190       1200 
QLSKEYALAE KKILLSSQDA KTDKIYLVLN THTLKEIKNT QFKETAFAKA LTVEVNNKEN 

      1210       1220       1230       1240       1250       1260 
QLQELLNHFN AHLKAKMDQN HITKLSFDTK WTAFVSDSRL YIPDFINIKL QDFISDPFKV 

      1270       1280       1290       1300       1310       1320 
ISQLMNKATN EMPYIQAEIT LKWLTKLIHD INKFCLSAIS EFGKEAIPFN YAALRDLEYQ 

      1330       1340       1350       1360       1370       1380 
INTKYVEIEN KVICNETVEN TKNIPKLTKL LKQLDPKRVA GGQEQYQTLM NKILTSETSM 

      1390       1400       1410       1420       1430       1440 
QQTYEKEQLK KEYFEIVNNV ASFKLAFNFP QQLQNVERLI EKFKSLPKSQ PFEKFPQEND 

      1450       1460       1470       1480       1490       1500 
LLSDSLNTEN YINGLRALLN FITAAQNYIQ NTLLKQWAVF QQQNFIPIDY SVANVKPISD 

      1510       1520       1530       1540       1550       1560 
LYARLRIERE RQVFYQVNSV FGTHLIVDDT GVPLQFHNIF NNAIVKFFSL NYKQINVPED 

      1570       1580       1590       1600       1610       1620 
TPRLVSSQYK LLSVCKSFIM ILQQFWENII TLDLGPYLRD GTQNFKRELI PIVNLKLFIY 

      1630       1640       1650       1660       1670       1680 
CITQAWTASE DSTVSTAFEL PIKQFTLLIL CSHPEYLYGC LSHSTDLVIN SLAKSIDKNS 

      1690       1700       1710       1720       1730       1740 
LYNTFVVSHN PPEKPMHLMR NICIDTQLWQ PAKLMKDTFQ QTFFTQLCPK NEKFFIYLTA 

      1750       1760       1770       1780       1790       1800 
FLILPYKFMN YIWIQYKPAI FTQRSYQNLI KDLCSEYVHQ NKITTSSVTP HEPDTIKSGE 

      1810       1820       1830       1840       1850       1860 
RITSKITVHK AQNTPTLTRL QAQEYVFDYI LYSFLTGYEM TFAMYIDIIE KTYLLCMRHL 

      1870       1880       1890       1900       1910       1920 
ENVLHDKDFQ SVLRARTFDI DYILKQSWTK NIVEHSLFSV QLDKIVSYLN HTNRATPNIP 

      1930       1940       1950       1960       1970       1980 
LILFNYDNEV VNVYLPPMST NPKKVAFYIK NPFHFPVQEY EATNLISFHL YPKTTDILNQ 

      1990       2000       2010       2020       2030       2040 
LPPNNTESTR PGKQTSETLT NKNLSEPKFK KPAVTGLMPK SQSIILSTDT NVPETSPDVK 

      2050       2060       2070 
ANTASAAIKD VTLAREKINE FSESINTTIS KLKSMYL 

« Hide

References

« Hide 'large scale' references
[1]"Nucleotide sequence analysis of a 38.5-kilobase-pair region of the genome of human herpesvirus 6 encoding human cytomegalovirus immediate-early gene homologs and transactivating functions."
Nicholas J., Martin M.E.D.
J. Virol. 68:597-610(1994) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution."
Gompels U.A., Nicholas J., Lawrence G.L., Jones M., Thomson B.J., Martin M.E.D., Efstathiou S., Craxton M.A., Macaulay H.A.
Virology 209:29-51(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
L25528 Genomic DNA. Translation: AAA16739.1. Different initiation.
X83413 Genomic DNA. Translation: CAA58411.1.
PIRT09326.

3D structure databases

ProteinModelPortalP52340.
ModBaseSearch...
MobiDBSearch...

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Phylogenomic databases

ProtClustDBCLSP2510059.

Family and domain databases

InterProIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEPS51521. HTUSP. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDEN_HHV6U
AccessionPrimary (citable) accession number: P52340
Secondary accession number(s): Q69055
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 26, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries