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P52340

- DEN_HHV6U

UniProt

P52340 - DEN_HHV6U

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Protein

Deneddylase

Gene

U31

Organism
Human herpesvirus 6A (strain Uganda-1102) (HHV-6 variant A) (Human B lymphotropic virus)
Status
Reviewed - Annotation score: 4 out of 5- Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains (By similarity).By similarity

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101Important for catalytic activityPROSITE-ProRule annotation
Active sitei23 – 231PROSITE-ProRule annotation
Active sitei156 – 1561PROSITE-ProRule annotation
Active sitei158 – 1581PROSITE-ProRule annotation

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Name:U31
Synonyms:HHRF1
OrganismiHuman herpesvirus 6A (strain Uganda-1102) (HHV-6 variant A) (Human B lymphotropic virus)
Taxonomic identifieri10370 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeRoseolovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009295: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-KW
  2. host cell nucleus Source: UniProtKB-KW
  3. viral tegument Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20772077DeneddylasePRO_0000116041Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins.By similarity

Structurei

3D structure databases

ProteinModelPortaliP52340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 221219Peptidase C76PROSITE-ProRule annotationAdd
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili496 – 52429Sequence AnalysisAdd
BLAST
Coiled coili1114 – 115441Sequence AnalysisAdd
BLAST
Coiled coili1185 – 121430Sequence AnalysisAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi280 – 2834Poly-Lys
Compositional biasi375 – 3784Poly-Gln

Sequence similaritiesi

Belongs to the herpesviridae deneddylase family.Curated
Contains 1 peptidase C76 domain.PROSITE-ProRule annotation

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52340-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MKIITSSTNQ NDSKYGPRAG KQCMSNSFSF LHTVYLNGIN NSLNAGTIDA
60 70 80 90 100
IMEEGYHLDT AGTLALMLNN SDSQDYRLPT EIPKRIHSRY GVTQHELSRP
110 120 130 140 150
FNGTLDTQKI DNEVYLGLID FILYGKSKNG PTFAVITIGV LSRALFFLNN
160 170 180 190 200
TLYLFDSHPT EREATAAIYI CQNIEEVYEL LTTHGTEGFY YDASLIFFIE
210 220 230 240 250
TSNLSLSSHD AELLILKTYK DPDIAIALDK FSSTEIHEIK KTDDIGSQQD
260 270 280 290 300
LVADKTTDLE HAPHKRKKNS HSLELELNDK KKKDTASLTY YATEVDLIPS
310 320 330 340 350
FYELRSQFQS LFHDLKSFPI MKSNFNWTIY LQDSPINPNQ PFATPFLWNR
360 370 380 390 400
VFHLLCQIID VFVGVGSTND DSSKQQQQTI FINYLLPFKD FSEVFNEALA
410 420 430 440 450
ACQENNLDII FIYNNYLCKT TTFRTLERIL LSKFLAIVDN DHKKHYEWVK
460 470 480 490 500
SWTTQMFQGM PKKLDDIENY LKAYVDHNPV KHFHEFICLN KAEKYKVAVL
510 520 530 540 550
LNEKRKEIQE AIEREKNSFA QLSNFIDKLG ETPALPIESE NVHKVHTSDI
560 570 580 590 600
TEGIVPRFIT ESIELPNIST LNNTQQISLD KQLNEKLTNT IHTLTNKFTK
610 620 630 640 650
IVQDNYNNIA AGFMPVTELN CLFAYLVNLY FNIEVLKHSG LNINTVLLQE
660 670 680 690 700
VEKLYDNTQF LRFGTSHFNI NNLSNFTLSI RKMFVDFYNS QKPSDRASEI
710 720 730 740 750
LAAIESILAD PSKNKTVVNI EMIKSQLEEL GKMEISTTEN KQTAAITKQI
760 770 780 790 800
LGDQELTPIY DFLHHLSAYN LPNTTTVKNL HLHFILEQRP DIAMTLHDKI
810 820 830 840 850
QSILDIYVDD MLNDITVPEQ TFSTVLFLVD LFPNSTEKTA LFESVLTLRQ
860 870 880 890 900
LAKKCANLKT LDEFDDLAQF ITTNSEQLQN MMRQHFGKKI PTLMGHIKFL
910 920 930 940 950
YSQKIITTEE KNWIQRAKTV VITSPEELTA FLATAPTKHA LQTCKPDLDK
960 970 980 990 1000
ALQRHMEEQM KQTAENDKKH ILTIRSTLEK RLTDILLILK DGQFSSLETM
1010 1020 1030 1040 1050
HLNLLETFLK QLQDNNVIIH FTHALLPVLK DIETTISKII SDVIEKILIK
1060 1070 1080 1090 1100
TPLNPEQMSK EEQKYTPLLS FLSKFKKTTF CTEDVKTEIE QVQKSITFLK
1110 1120 1130 1140 1150
KIATSTNKHT RLSHSIYGQE LNLYEERITE LRKETNKMKE QLSKEYALAE
1160 1170 1180 1190 1200
KKILLSSQDA KTDKIYLVLN THTLKEIKNT QFKETAFAKA LTVEVNNKEN
1210 1220 1230 1240 1250
QLQELLNHFN AHLKAKMDQN HITKLSFDTK WTAFVSDSRL YIPDFINIKL
1260 1270 1280 1290 1300
QDFISDPFKV ISQLMNKATN EMPYIQAEIT LKWLTKLIHD INKFCLSAIS
1310 1320 1330 1340 1350
EFGKEAIPFN YAALRDLEYQ INTKYVEIEN KVICNETVEN TKNIPKLTKL
1360 1370 1380 1390 1400
LKQLDPKRVA GGQEQYQTLM NKILTSETSM QQTYEKEQLK KEYFEIVNNV
1410 1420 1430 1440 1450
ASFKLAFNFP QQLQNVERLI EKFKSLPKSQ PFEKFPQEND LLSDSLNTEN
1460 1470 1480 1490 1500
YINGLRALLN FITAAQNYIQ NTLLKQWAVF QQQNFIPIDY SVANVKPISD
1510 1520 1530 1540 1550
LYARLRIERE RQVFYQVNSV FGTHLIVDDT GVPLQFHNIF NNAIVKFFSL
1560 1570 1580 1590 1600
NYKQINVPED TPRLVSSQYK LLSVCKSFIM ILQQFWENII TLDLGPYLRD
1610 1620 1630 1640 1650
GTQNFKRELI PIVNLKLFIY CITQAWTASE DSTVSTAFEL PIKQFTLLIL
1660 1670 1680 1690 1700
CSHPEYLYGC LSHSTDLVIN SLAKSIDKNS LYNTFVVSHN PPEKPMHLMR
1710 1720 1730 1740 1750
NICIDTQLWQ PAKLMKDTFQ QTFFTQLCPK NEKFFIYLTA FLILPYKFMN
1760 1770 1780 1790 1800
YIWIQYKPAI FTQRSYQNLI KDLCSEYVHQ NKITTSSVTP HEPDTIKSGE
1810 1820 1830 1840 1850
RITSKITVHK AQNTPTLTRL QAQEYVFDYI LYSFLTGYEM TFAMYIDIIE
1860 1870 1880 1890 1900
KTYLLCMRHL ENVLHDKDFQ SVLRARTFDI DYILKQSWTK NIVEHSLFSV
1910 1920 1930 1940 1950
QLDKIVSYLN HTNRATPNIP LILFNYDNEV VNVYLPPMST NPKKVAFYIK
1960 1970 1980 1990 2000
NPFHFPVQEY EATNLISFHL YPKTTDILNQ LPPNNTESTR PGKQTSETLT
2010 2020 2030 2040 2050
NKNLSEPKFK KPAVTGLMPK SQSIILSTDT NVPETSPDVK ANTASAAIKD
2060 2070
VTLAREKINE FSESINTTIS KLKSMYL
Length:2,077
Mass (Da):239,947
Last modified:October 1, 1996 - v1
Checksum:iC1CA4BDC26650511
GO

Sequence cautioni

The sequence AAA16739.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25528 Genomic DNA. Translation: AAA16739.1. Different initiation.
X83413 Genomic DNA. Translation: CAA58411.1.
PIRiT09326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L25528 Genomic DNA. Translation: AAA16739.1 . Different initiation.
X83413 Genomic DNA. Translation: CAA58411.1 .
PIRi T09326.

3D structure databases

ProteinModelPortali P52340.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of a 38.5-kilobase-pair region of the genome of human herpesvirus 6 encoding human cytomegalovirus immediate-early gene homologs and transactivating functions."
    Nicholas J., Martin M.E.D.
    J. Virol. 68:597-610(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution."
    Gompels U.A., Nicholas J., Lawrence G.L., Jones M., Thomson B.J., Martin M.E.D., Efstathiou S., Craxton M.A., Macaulay H.A.
    Virology 209:29-51(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_HHV6U
AccessioniPrimary (citable) accession number: P52340
Secondary accession number(s): Q69055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 57 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3