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P52340

- DEN_HHV6U

UniProt

P52340 - DEN_HHV6U

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Protein

Deneddylase

Gene
U31, HHRF1
Organism
Human herpesvirus 6A (strain Uganda-1102) (HHV-6 variant A) (Human B lymphotropic virus)
Status
Reviewed - Annotation score: 4 out of 5 - Protein inferred from homologyi

Functioni

Deneddylase that deregulates the host cell cycle S phase to create a favorable environment allowing efficient viral genome replication. Interacts with and deneddylates host cullins including CUL1 and CUL4A, thereby reducing their E3 ubiquitin ligase activity. Inhibition of cullins leads to the stabilization of cullin-RING ligase substrates such as host CDN1A/p21, CDKN1B/p27kip and CDC25A, preventing S phase progression. Additionally, acts as a deubiquitinase and cleaves both 'Lys-48' and 'Lys-63'-linked ubiquitin chains By similarity.

Catalytic activityi

Thiol-dependent hydrolysis of ester, thioester, amide, peptide and isopeptide bonds formed by the C-terminal Gly of ubiquitin (a 76-residue protein attached to proteins as an intracellular targeting signal).

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei10 – 101Important for catalytic activity By similarity
Active sitei23 – 231 By similarity
Active sitei156 – 1561 By similarity
Active sitei158 – 1581 By similarity

GO - Molecular functioni

  1. cysteine-type peptidase activity Source: UniProtKB-KW

GO - Biological processi

  1. modulation by virus of host protein ubiquitination Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Thiol protease

Keywords - Biological processi

Host-virus interaction, Modulation of host ubiquitin pathway by viral deubiquitinase, Modulation of host ubiquitin pathway by virus, Ubl conjugation pathway

Names & Taxonomyi

Protein namesi
Recommended name:
Deneddylase (EC:3.4.19.12, EC:3.4.22.-)
Gene namesi
Name:U31
Synonyms:HHRF1
OrganismiHuman herpesvirus 6A (strain Uganda-1102) (HHV-6 variant A) (Human B lymphotropic virus)
Taxonomic identifieri10370 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stageHerpesviralesHerpesviridaeBetaherpesvirinaeRoseolovirus
Virus hostiHomo sapiens (Human) [TaxID: 9606]
ProteomesiUP000009295: Genome

Subcellular locationi

GO - Cellular componenti

  1. host cell cytoplasm Source: UniProtKB-SubCell
  2. host cell nucleus Source: UniProtKB-SubCell
  3. viral tegument Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Host cytoplasm, Host nucleus, Virion, Virion tegument

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 20772077DeneddylasePRO_0000116041Add
BLAST

Interactioni

Subunit structurei

Interacts with host CUL1 and CUL4A; these interactions inhibit the E3 ligase activity of cullins By similarity.

Structurei

3D structure databases

ProteinModelPortaliP52340.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini3 – 221219Peptidase C76Add
BLAST

Coiled coil

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Coiled coili496 – 52429 Reviewed predictionAdd
BLAST
Coiled coili1114 – 115441 Reviewed predictionAdd
BLAST
Coiled coili1185 – 121430 Reviewed predictionAdd
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi280 – 2834Poly-Lys
Compositional biasi375 – 3784Poly-Gln

Sequence similaritiesi

Keywords - Domaini

Coiled coil, Repeat

Family and domain databases

InterProiIPR006928. Pept_C76_UL36-USP.
[Graphical view]
PfamiPF04843. Herpes_teg_N. 1 hit.
[Graphical view]
PROSITEiPS51521. HTUSP. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52340-1 [UniParc]FASTAAdd to Basket

« Hide

MKIITSSTNQ NDSKYGPRAG KQCMSNSFSF LHTVYLNGIN NSLNAGTIDA     50
IMEEGYHLDT AGTLALMLNN SDSQDYRLPT EIPKRIHSRY GVTQHELSRP 100
FNGTLDTQKI DNEVYLGLID FILYGKSKNG PTFAVITIGV LSRALFFLNN 150
TLYLFDSHPT EREATAAIYI CQNIEEVYEL LTTHGTEGFY YDASLIFFIE 200
TSNLSLSSHD AELLILKTYK DPDIAIALDK FSSTEIHEIK KTDDIGSQQD 250
LVADKTTDLE HAPHKRKKNS HSLELELNDK KKKDTASLTY YATEVDLIPS 300
FYELRSQFQS LFHDLKSFPI MKSNFNWTIY LQDSPINPNQ PFATPFLWNR 350
VFHLLCQIID VFVGVGSTND DSSKQQQQTI FINYLLPFKD FSEVFNEALA 400
ACQENNLDII FIYNNYLCKT TTFRTLERIL LSKFLAIVDN DHKKHYEWVK 450
SWTTQMFQGM PKKLDDIENY LKAYVDHNPV KHFHEFICLN KAEKYKVAVL 500
LNEKRKEIQE AIEREKNSFA QLSNFIDKLG ETPALPIESE NVHKVHTSDI 550
TEGIVPRFIT ESIELPNIST LNNTQQISLD KQLNEKLTNT IHTLTNKFTK 600
IVQDNYNNIA AGFMPVTELN CLFAYLVNLY FNIEVLKHSG LNINTVLLQE 650
VEKLYDNTQF LRFGTSHFNI NNLSNFTLSI RKMFVDFYNS QKPSDRASEI 700
LAAIESILAD PSKNKTVVNI EMIKSQLEEL GKMEISTTEN KQTAAITKQI 750
LGDQELTPIY DFLHHLSAYN LPNTTTVKNL HLHFILEQRP DIAMTLHDKI 800
QSILDIYVDD MLNDITVPEQ TFSTVLFLVD LFPNSTEKTA LFESVLTLRQ 850
LAKKCANLKT LDEFDDLAQF ITTNSEQLQN MMRQHFGKKI PTLMGHIKFL 900
YSQKIITTEE KNWIQRAKTV VITSPEELTA FLATAPTKHA LQTCKPDLDK 950
ALQRHMEEQM KQTAENDKKH ILTIRSTLEK RLTDILLILK DGQFSSLETM 1000
HLNLLETFLK QLQDNNVIIH FTHALLPVLK DIETTISKII SDVIEKILIK 1050
TPLNPEQMSK EEQKYTPLLS FLSKFKKTTF CTEDVKTEIE QVQKSITFLK 1100
KIATSTNKHT RLSHSIYGQE LNLYEERITE LRKETNKMKE QLSKEYALAE 1150
KKILLSSQDA KTDKIYLVLN THTLKEIKNT QFKETAFAKA LTVEVNNKEN 1200
QLQELLNHFN AHLKAKMDQN HITKLSFDTK WTAFVSDSRL YIPDFINIKL 1250
QDFISDPFKV ISQLMNKATN EMPYIQAEIT LKWLTKLIHD INKFCLSAIS 1300
EFGKEAIPFN YAALRDLEYQ INTKYVEIEN KVICNETVEN TKNIPKLTKL 1350
LKQLDPKRVA GGQEQYQTLM NKILTSETSM QQTYEKEQLK KEYFEIVNNV 1400
ASFKLAFNFP QQLQNVERLI EKFKSLPKSQ PFEKFPQEND LLSDSLNTEN 1450
YINGLRALLN FITAAQNYIQ NTLLKQWAVF QQQNFIPIDY SVANVKPISD 1500
LYARLRIERE RQVFYQVNSV FGTHLIVDDT GVPLQFHNIF NNAIVKFFSL 1550
NYKQINVPED TPRLVSSQYK LLSVCKSFIM ILQQFWENII TLDLGPYLRD 1600
GTQNFKRELI PIVNLKLFIY CITQAWTASE DSTVSTAFEL PIKQFTLLIL 1650
CSHPEYLYGC LSHSTDLVIN SLAKSIDKNS LYNTFVVSHN PPEKPMHLMR 1700
NICIDTQLWQ PAKLMKDTFQ QTFFTQLCPK NEKFFIYLTA FLILPYKFMN 1750
YIWIQYKPAI FTQRSYQNLI KDLCSEYVHQ NKITTSSVTP HEPDTIKSGE 1800
RITSKITVHK AQNTPTLTRL QAQEYVFDYI LYSFLTGYEM TFAMYIDIIE 1850
KTYLLCMRHL ENVLHDKDFQ SVLRARTFDI DYILKQSWTK NIVEHSLFSV 1900
QLDKIVSYLN HTNRATPNIP LILFNYDNEV VNVYLPPMST NPKKVAFYIK 1950
NPFHFPVQEY EATNLISFHL YPKTTDILNQ LPPNNTESTR PGKQTSETLT 2000
NKNLSEPKFK KPAVTGLMPK SQSIILSTDT NVPETSPDVK ANTASAAIKD 2050
VTLAREKINE FSESINTTIS KLKSMYL 2077
Length:2,077
Mass (Da):239,947
Last modified:October 1, 1996 - v1
Checksum:iC1CA4BDC26650511
GO

Sequence cautioni

The sequence AAA16739.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25528 Genomic DNA. Translation: AAA16739.1. Different initiation.
X83413 Genomic DNA. Translation: CAA58411.1.
PIRiT09326.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
L25528 Genomic DNA. Translation: AAA16739.1 . Different initiation.
X83413 Genomic DNA. Translation: CAA58411.1 .
PIRi T09326.

3D structure databases

ProteinModelPortali P52340.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

InterProi IPR006928. Pept_C76_UL36-USP.
[Graphical view ]
Pfami PF04843. Herpes_teg_N. 1 hit.
[Graphical view ]
PROSITEi PS51521. HTUSP. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. "Nucleotide sequence analysis of a 38.5-kilobase-pair region of the genome of human herpesvirus 6 encoding human cytomegalovirus immediate-early gene homologs and transactivating functions."
    Nicholas J., Martin M.E.D.
    J. Virol. 68:597-610(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "The DNA sequence of human herpesvirus-6: structure, coding content, and genome evolution."
    Gompels U.A., Nicholas J., Lawrence G.L., Jones M., Thomson B.J., Martin M.E.D., Efstathiou S., Craxton M.A., Macaulay H.A.
    Virology 209:29-51(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Entry informationi

Entry nameiDEN_HHV6U
AccessioniPrimary (citable) accession number: P52340
Secondary accession number(s): Q69055
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 14, 2014
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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