Tyrosine-protein kinase JAK1 - Mus musculus (Mouse)

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P52332 (JAK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified May 1, 2013. Version 123. History...

Clusters with 100%, 90%, 50% identity |

Documents (3) |

Third-party data

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Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents Customize order

Names and origin

Protein names

Recommended name:
Tyrosine-protein kinase JAK1
EC=2.7.10.2

Alternative name(s):
Janus kinase 1
Short name=JAK-1

Gene names

Name:

Jak1

Organism

Mus musculus (Mouse) [Reference proteome]

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus › Mus

Protein attributes

Sequence length	1153 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with IL31RA, IFNAR2, JAKMIP1 and SHB By similarity. Interacts with FER. Ref.5
Subcellular location	Endomembrane system; Peripheral membrane protein. Note: Wholly intracellular, possibly membrane associated.
Domain	The FERM domain mediates interaction with JAKMIP1 By similarity. Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 2 protein kinase domains. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Acetylation Phosphoprotein
Technical term	Complete proteome Direct protein sequencing Reference proteome
Gene Ontology (GO)
Biological_process	cytokine-mediated signaling pathway Inferred from direct assay PubMed 8022486. Source: MGI enzyme linked receptor protein signaling pathway Inferred from direct assay PubMed 8022486. Source: MGI intracellular protein kinase cascade Inferred from direct assay PubMed 8022486. Source: MGI peptidyl-tyrosine phosphorylation Inferred from direct assay PubMed 10872802. Source: MGI response to antibiotic Inferred from sequence orthology PubMed 16280321. Source: MGI
Cellular_component	cytoskeleton Inferred from electronic annotation. Source: InterPro cytosol Traceable author statement. Source: Reactome endomembrane system Inferred from electronic annotation. Source: UniProtKB-SubCell membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC protein tyrosine kinase activity Inferred from direct assay PubMed 10872802. Source: MGI
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Chain

1 – 1153

1153

Tyrosine-protein kinase JAK1

PRO_0000088109

Regions

Domain

34 – 420

387

FERM

Domain

439 – 542

104

SH2

Domain

582 – 854

273

Protein kinase 1

Domain

874 – 1152

279

Protein kinase 2

Nucleotide binding

880 – 888

ATP By similarity

Sites

Active site

1002

Proton acceptor By similarity

Binding site

907

ATP By similarity

Amino acid modifications

Modified residue

N-acetylmethionine By similarity

Modified residue

Phosphotyrosine By similarity

Modified residue

228

Phosphoserine By similarity

Modified residue

1033

Phosphotyrosine; by autocatalysis By similarity

Experimental info

Sequence conflict

999

V → I Ref.3

Sequence conflict

999

V → I Ref.4

Sequence conflict

1068 – 1069

VT → IP Ref.3

Sequence conflict

1068 – 1069

VT → IP Ref.4

Sequences

Sequence

Length

Mass (Da)

Tools

P52332 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 0B9816AEA5B3868B
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FASTA

1,153

133,367

        10         20         30         40         50         60 
MQYLNIKEDC NAMAFCAKMR SFKKTEVKQV VPEPGVEVTF YLLDREPLRL GSGEYTAEEL 

        70         80         90        100        110        120 
CIRAAQECSI SPLCHNLFAL YDESTKLWYA PNRIITVDDK TSLRLHYRMR FYFTNWHGTN 

       130        140        150        160        170        180 
DNEQSVWRHS PKKQKNGYEK KRVPEATPLL DASSLEYLFA QGQYDLIKCL APIRDPKTEQ 

       190        200        210        220        230        240 
DGHDIENECL GMAVLAISHY AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI 

       250        260        270        280        290        300 
NNVFKDFLKE FNNKTICDSS VHDLKVKYLA TLETSTLTKH YGAEIFETSM LLISSENELS 

       310        320        330        340        350        360 
RCHSNDSGNV LYEVMVTGNL GIQWRQKPNV VPVEKEKNKL KRKKLEYNKH KKDDERNKLR 

       370        380        390        400        410        420 
EEWNNFSYFP EITHIVIKES VVSINKQDNK NMELKLSSRE EALSFVSLVD GYFRLTADAH 

       430        440        450        460        470        480 
HYLCTDVAPP LIVHNIQNGC HGPICTEYAI NKLRQEGSEE GMYVLRWSCT DFDNILMTVT 

       490        500        510        520        530        540 
CFEKSEVLGG QKQFKNFQIE VQKGRYSLHG SMDHFPSLRD LMNHLKKQIL RTDNISFVLK 

       550        560        570        580        590        600 
RCCQPKPREI SNLLVATKKA QEWQPVYSMS QLSFDRILKK DIIQGEHLGR GTRTHIYSGT 

       610        620        630        640        650        660 
LLDYKDEEGI AEEKKIKVIL KVLDPSHRDI SLAFFEAASM MRQVSHKHIV YLYGVCVRDV 

       670        680        690        700        710        720 
ENIMVEEFVE GGPLDLFMHR KSDALTTPWK FKVAKQLASA LSYLEDKDLV HGNVCTKNLL 

       730        740        750        760        770        780 
LAREGIDSDI GPFIKLSDPG IPVSVLTRQE CIERIPWIAP ECVEDSKNLS VAADKWSFGT 

       790        800        810        820        830        840 
TLWEICYNGE IPLKDKTLIE KERFYESRCR PVTPSCKELA DLMTRCMNYD PNQRPFFRAI 

       850        860        870        880        890        900 
MRDINKLEEQ NPDIVSEKQP TTEVDPTHFE KRFLKRIRDL GEGHFGKVEL CRYDPEGDNT 

       910        920        930        940        950        960 
GEQVAVKSLK PESGGNHIAD LKKEIEILRN LYHENIVKYK GICMEDGGNG IKLIMEFLPS 

       970        980        990       1000       1010       1020 
GSLKEYLPKN KNKINLKQQL KYAIQICKGM DYLGSRQYVH RDLAARNVLV ESEHQVKIGD 

      1030       1040       1050       1060       1070       1080 
FGLTKAIETD KEYYTVKDDR DSPVFWYAPE CLIQCKFYIA SDVWSFGVTL HELLTYCDSD 

      1090       1100       1110       1120       1130       1140 
FSPMALFLKM IGPTHGQMTV TRLVKTLKEG KRLPCPPNCP DEVYQLMRKC WEFQPSNRTT 

      1150 
FQNLIEGFEA LLK

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References

[1]	"Molecular cloning of the murine JAK1 protein tyrosine kinase and its expression in the mouse central nervous system." Yang X., Chung D., Cepko C.L. J. Neurosci. 13:3006-3017(1993) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 718-723, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[3]	"The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family." Wilks A.F., Kurban R.R., Hovens C.M., Ralph S.J. Gene 85:67-74(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
[4]	"Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction." Wilks A.F. Proc. Natl. Acad. Sci. U.S.A. 86:1603-1607(1989) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
[5]	"Fer is a downstream effector of insulin and mediates the activation of signal transducer and activator of transcription 3 in myogenic cells." Taler M., Shpungin S., Salem Y., Malovani H., Pasder O., Nir U. Mol. Endocrinol. 17:1580-1592(2003) [PubMed] [Europe PMC] [Abstract] Cited for: INTERACTION WITH FER.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	S63728 mRNA. Translation: AAB27517.2. M33425 mRNA. Translation: AAA40016.1.
IPI	IPI00126078.
UniGene	Mm.289657.
3D structure databases
ProteinModelPortal	P52332.
SMR	P52332. Positions 495-1153.
ModBase	Search...
Protein-protein interaction databases
DIP	DIP-41965N.
IntAct	P52332. 1 interaction.
MINT	MINT-188445.
PTM databases
PhosphoSite	P52332.
Proteomic databases
PaxDb	P52332.
PRIDE	P52332.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Organism-specific databases
MGI	MGI:96628. Jak1.
Phylogenomic databases
eggNOG	COG0515.
HOGENOM	HOG000049158.
HOVERGEN	HBG006195.
InParanoid	P52332.
OrthoDB	EOG4PVNXT.
Enzyme and pathway databases
BRENDA	2.7.10.2. 3474.
Reactome	REACT_107772. Immune System.
Gene expression databases
CleanEx	MM_JAK1.
Genevestigator	P52332.
GermOnline	ENSMUSG00000028530. Mus musculus.
Family and domain databases
Gene3D	3.30.505.10. 1 hit.
InterPro	IPR019749. Band_41_domain. IPR019748. FERM_central. IPR000299. FERM_domain. IPR011009. Kinase-like_dom. IPR000719. Prot_kinase_cat_dom. IPR017441. Protein_kinase_ATP_BS. IPR001245. Ser-Thr/Tyr_kinase_cat_dom. IPR000980. SH2. IPR008266. Tyr_kinase_AS. IPR020635. Tyr_kinase_cat_dom. IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2. IPR020776. Tyr_kinase_non-rcpt_Jak1. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 2 hits. [Graphical view]
PIRSF	PIRSF000636. TyrPK_Jak. 1 hit.
PRINTS	PR01823. JANUSKINASE. PR01824. JANUSKINASE1. PR00109. TYRKINASE.
SMART	SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view]
SUPFAM	SSF47031. FERM_3-hlx. 1 hit. SSF56112. Kinase_like. 2 hits.
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other
BindingDB	P52332.
ChEMBL	CHEMBL2968.
ChiTaRS	JAK1. mouse.
SOURCE	Search...

Entry information

Entry name

JAK1_MOUSE

Accession

Primary (citable) accession number: P52332
Secondary accession number(s): Q62126

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	May 1, 2013
This is version 123 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program

Relevant documents

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot

SIMILARITY comments

Index of protein domains and families

Names·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents

Preferred order of sections

Names and origin

Protein attributes

General annotation (Comments)

Ontologies

Sequence annotation (Features)

Molecule processing

Regions

Sites

Amino acid modifications

Experimental info

Sequences

References

Cross-references

Sequence databases

3D structure databases

Protein-protein interaction databases

PTM databases

Proteomic databases

Protocols and materials databases

Organism-specific databases

Phylogenomic databases

Enzyme and pathway databases

Gene expression databases

Family and domain databases

Other

Entry information

Relevant documents