Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

Tyrosine-protein kinase JAK1

Gene

Jak1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Cofactori

Mg2+CuratedNote: Mn2+ was used in the in vitro kinase assay but Mg2+ is likely to be the in vivo cofactor.Curated

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei907ATPPROSITE-ProRule annotation1
Active sitei1002Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi880 – 888ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiR-MMU-1266695. Interleukin-7 signaling.
SABIO-RKP52332.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
Alternative name(s):
Janus kinase 1
Short name:
JAK-1
Gene namesi
Name:Jak1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96628. Jak1.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2968.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881091 – 1153Tyrosine-protein kinase JAK1Add BLAST1153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3PhosphotyrosineBy similarity1
Modified residuei228PhosphoserineBy similarity1
Modified residuei1033Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1034Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1033 and Tyr-1034 by PTPN2 negatively regulates cytokine-mediated signaling.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

EPDiP52332.
MaxQBiP52332.
PaxDbiP52332.
PeptideAtlasiP52332.
PRIDEiP52332.

PTM databases

iPTMnetiP52332.
PhosphoSitePlusiP52332.
SwissPalmiP52332.

Expressioni

Gene expression databases

CleanExiMM_JAK1.

Interactioni

Subunit structurei

Interacts with IL31RA, IFNAR2, IFNGR1, JAKMIP1 and SHB (By similarity). Interacts with FER (PubMed:12738762).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

DIPiDIP-41965N.
IntActiP52332. 3 interactors.
MINTiMINT-188445.
STRINGi10090.ENSMUSP00000099842.

Chemistry databases

BindingDBiP52332.

Structurei

3D structure databases

ProteinModelPortaliP52332.
SMRiP52332.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 420FERMPROSITE-ProRule annotationAdd BLAST387
Domaini439 – 542SH2PROSITE-ProRule annotationAdd BLAST104
Domaini582 – 854Protein kinase 1PROSITE-ProRule annotationAdd BLAST273
Domaini874 – 1152Protein kinase 2PROSITE-ProRule annotationAdd BLAST279

Domaini

The FERM domain mediates interaction with JAKMIP1.By similarity
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation
Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 2 protein kinase domains.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000049158.
HOVERGENiHBG006195.
InParanoidiP52332.
PhylomeDBiP52332.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSiPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTiSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYLNIKEDC NAMAFCAKMR SFKKTEVKQV VPEPGVEVTF YLLDREPLRL
60 70 80 90 100
GSGEYTAEEL CIRAAQECSI SPLCHNLFAL YDESTKLWYA PNRIITVDDK
110 120 130 140 150
TSLRLHYRMR FYFTNWHGTN DNEQSVWRHS PKKQKNGYEK KRVPEATPLL
160 170 180 190 200
DASSLEYLFA QGQYDLIKCL APIRDPKTEQ DGHDIENECL GMAVLAISHY
210 220 230 240 250
AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI NNVFKDFLKE
260 270 280 290 300
FNNKTICDSS VHDLKVKYLA TLETSTLTKH YGAEIFETSM LLISSENELS
310 320 330 340 350
RCHSNDSGNV LYEVMVTGNL GIQWRQKPNV VPVEKEKNKL KRKKLEYNKH
360 370 380 390 400
KKDDERNKLR EEWNNFSYFP EITHIVIKES VVSINKQDNK NMELKLSSRE
410 420 430 440 450
EALSFVSLVD GYFRLTADAH HYLCTDVAPP LIVHNIQNGC HGPICTEYAI
460 470 480 490 500
NKLRQEGSEE GMYVLRWSCT DFDNILMTVT CFEKSEVLGG QKQFKNFQIE
510 520 530 540 550
VQKGRYSLHG SMDHFPSLRD LMNHLKKQIL RTDNISFVLK RCCQPKPREI
560 570 580 590 600
SNLLVATKKA QEWQPVYSMS QLSFDRILKK DIIQGEHLGR GTRTHIYSGT
610 620 630 640 650
LLDYKDEEGI AEEKKIKVIL KVLDPSHRDI SLAFFEAASM MRQVSHKHIV
660 670 680 690 700
YLYGVCVRDV ENIMVEEFVE GGPLDLFMHR KSDALTTPWK FKVAKQLASA
710 720 730 740 750
LSYLEDKDLV HGNVCTKNLL LAREGIDSDI GPFIKLSDPG IPVSVLTRQE
760 770 780 790 800
CIERIPWIAP ECVEDSKNLS VAADKWSFGT TLWEICYNGE IPLKDKTLIE
810 820 830 840 850
KERFYESRCR PVTPSCKELA DLMTRCMNYD PNQRPFFRAI MRDINKLEEQ
860 870 880 890 900
NPDIVSEKQP TTEVDPTHFE KRFLKRIRDL GEGHFGKVEL CRYDPEGDNT
910 920 930 940 950
GEQVAVKSLK PESGGNHIAD LKKEIEILRN LYHENIVKYK GICMEDGGNG
960 970 980 990 1000
IKLIMEFLPS GSLKEYLPKN KNKINLKQQL KYAIQICKGM DYLGSRQYVH
1010 1020 1030 1040 1050
RDLAARNVLV ESEHQVKIGD FGLTKAIETD KEYYTVKDDR DSPVFWYAPE
1060 1070 1080 1090 1100
CLIQCKFYIA SDVWSFGVTL HELLTYCDSD FSPMALFLKM IGPTHGQMTV
1110 1120 1130 1140 1150
TRLVKTLKEG KRLPCPPNCP DEVYQLMRKC WEFQPSNRTT FQNLIEGFEA

LLK
Length:1,153
Mass (Da):133,367
Last modified:October 1, 1996 - v1
Checksum:i0B9816AEA5B3868B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti999V → I (PubMed:2482828).Curated1
Sequence conflicti999V → I (PubMed:2466296).Curated1
Sequence conflicti1068 – 1069VT → IP (PubMed:2482828).Curated2
Sequence conflicti1068 – 1069VT → IP (PubMed:2466296).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63728 mRNA. Translation: AAB27517.2.
M33425 mRNA. Translation: AAA40016.1.
UniGeneiMm.289657.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63728 mRNA. Translation: AAB27517.2.
M33425 mRNA. Translation: AAA40016.1.
UniGeneiMm.289657.

3D structure databases

ProteinModelPortaliP52332.
SMRiP52332.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

DIPiDIP-41965N.
IntActiP52332. 3 interactors.
MINTiMINT-188445.
STRINGi10090.ENSMUSP00000099842.

Chemistry databases

BindingDBiP52332.
ChEMBLiCHEMBL2968.

PTM databases

iPTMnetiP52332.
PhosphoSitePlusiP52332.
SwissPalmiP52332.

Proteomic databases

EPDiP52332.
MaxQBiP52332.
PaxDbiP52332.
PeptideAtlasiP52332.
PRIDEiP52332.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Organism-specific databases

MGIiMGI:96628. Jak1.

Phylogenomic databases

eggNOGiKOG0197. Eukaryota.
COG0515. LUCA.
HOGENOMiHOG000049158.
HOVERGENiHBG006195.
InParanoidiP52332.
PhylomeDBiP52332.

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiR-MMU-1266695. Interleukin-7 signaling.
SABIO-RKP52332.

Miscellaneous databases

ChiTaRSiJak1. mouse.
PROiP52332.
SOURCEiSearch...

Gene expression databases

CleanExiMM_JAK1.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR011993. PH_dom-like.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSiPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTiSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF50729. SSF50729. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiJAK1_MOUSE
AccessioniPrimary (citable) accession number: P52332
Secondary accession number(s): Q62126
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 157 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.