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P52332

- JAK1_MOUSE

UniProt

P52332 - JAK1_MOUSE

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Protein

Tyrosine-protein kinase JAK1

Gene

Jak1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei907 – 9071ATPPROSITE-ProRule annotation
Active sitei1002 – 10021Proton acceptorPROSITE-ProRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi880 – 8889ATPPROSITE-ProRule annotation

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-KW
  2. growth hormone receptor binding Source: BHF-UCL
  3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
  4. protein tyrosine kinase activity Source: MGI

GO - Biological processi

  1. cytokine-mediated signaling pathway Source: MGI
  2. enzyme linked receptor protein signaling pathway Source: MGI
  3. interleukin-2-mediated signaling pathway Source: UniProtKB
  4. intracellular signal transduction Source: MGI
  5. peptidyl-tyrosine phosphorylation Source: MGI
  6. response to antibiotic Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase, Tyrosine-protein kinase

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2. 3474.
ReactomeiREACT_198701. Interleukin-7 signaling.
SABIO-RKP52332.

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
Alternative name(s):
Janus kinase 1
Short name:
JAK-1
Gene namesi
Name:Jak1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
ProteomesiUP000000589: Unplaced

Organism-specific databases

MGIiMGI:96628. Jak1.

Subcellular locationi

Endomembrane system; Peripheral membrane protein
Note: Wholly intracellular, possibly membrane associated.

GO - Cellular componenti

  1. cytoskeleton Source: InterPro
  2. cytosol Source: Reactome
  3. membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 11531153Tyrosine-protein kinase JAK1PRO_0000088109Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei3 – 31PhosphotyrosineBy similarity
Modified residuei228 – 2281PhosphoserineBy similarity
Modified residuei1033 – 10331Phosphotyrosine; by autocatalysisBy similarity
Modified residuei1034 – 10341Phosphotyrosine; by autocatalysisBy similarity

Post-translational modificationi

Phosphorylated. Dephosphorylation of Tyr-1033 and Tyr-1034 by PTPN2 negatively regulates cytokine-mediated signaling (By similarity).By similarity

Keywords - PTMi

Acetylation, Phosphoprotein

Proteomic databases

MaxQBiP52332.
PaxDbiP52332.
PRIDEiP52332.

PTM databases

PhosphoSiteiP52332.

Expressioni

Gene expression databases

CleanExiMM_JAK1.
GenevestigatoriP52332.

Interactioni

Subunit structurei

Interacts with IL31RA, IFNAR2, JAKMIP1 and SHB (By similarity). Interacts with FER.By similarity1 Publication

Protein-protein interaction databases

DIPiDIP-41965N.
IntActiP52332. 2 interactions.
MINTiMINT-188445.

Structurei

3D structure databases

ProteinModelPortaliP52332.
SMRiP52332. Positions 515-1153.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini34 – 420387FERMPROSITE-ProRule annotationAdd
BLAST
Domaini439 – 542104SH2PROSITE-ProRule annotationAdd
BLAST
Domaini582 – 854273Protein kinase 1PROSITE-ProRule annotationAdd
BLAST
Domaini874 – 1152279Protein kinase 2PROSITE-ProRule annotationAdd
BLAST

Domaini

The FERM domain mediates interaction with JAKMIP1.By similarity
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain (By similarity), while the presence of slight differences suggest a different role for domain 1.

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation
Contains 1 FERM domain.PROSITE-ProRule annotation
Contains 2 protein kinase domains.PROSITE-ProRule annotation
Contains 1 SH2 domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiCOG0515.
HOGENOMiHOG000049158.
HOVERGENiHBG006195.
InParanoidiP52332.
PhylomeDBiP52332.

Family and domain databases

Gene3Di3.30.505.10. 1 hit.
InterProiIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamiPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSiPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTiSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMiSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEiPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52332 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
MQYLNIKEDC NAMAFCAKMR SFKKTEVKQV VPEPGVEVTF YLLDREPLRL
60 70 80 90 100
GSGEYTAEEL CIRAAQECSI SPLCHNLFAL YDESTKLWYA PNRIITVDDK
110 120 130 140 150
TSLRLHYRMR FYFTNWHGTN DNEQSVWRHS PKKQKNGYEK KRVPEATPLL
160 170 180 190 200
DASSLEYLFA QGQYDLIKCL APIRDPKTEQ DGHDIENECL GMAVLAISHY
210 220 230 240 250
AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI NNVFKDFLKE
260 270 280 290 300
FNNKTICDSS VHDLKVKYLA TLETSTLTKH YGAEIFETSM LLISSENELS
310 320 330 340 350
RCHSNDSGNV LYEVMVTGNL GIQWRQKPNV VPVEKEKNKL KRKKLEYNKH
360 370 380 390 400
KKDDERNKLR EEWNNFSYFP EITHIVIKES VVSINKQDNK NMELKLSSRE
410 420 430 440 450
EALSFVSLVD GYFRLTADAH HYLCTDVAPP LIVHNIQNGC HGPICTEYAI
460 470 480 490 500
NKLRQEGSEE GMYVLRWSCT DFDNILMTVT CFEKSEVLGG QKQFKNFQIE
510 520 530 540 550
VQKGRYSLHG SMDHFPSLRD LMNHLKKQIL RTDNISFVLK RCCQPKPREI
560 570 580 590 600
SNLLVATKKA QEWQPVYSMS QLSFDRILKK DIIQGEHLGR GTRTHIYSGT
610 620 630 640 650
LLDYKDEEGI AEEKKIKVIL KVLDPSHRDI SLAFFEAASM MRQVSHKHIV
660 670 680 690 700
YLYGVCVRDV ENIMVEEFVE GGPLDLFMHR KSDALTTPWK FKVAKQLASA
710 720 730 740 750
LSYLEDKDLV HGNVCTKNLL LAREGIDSDI GPFIKLSDPG IPVSVLTRQE
760 770 780 790 800
CIERIPWIAP ECVEDSKNLS VAADKWSFGT TLWEICYNGE IPLKDKTLIE
810 820 830 840 850
KERFYESRCR PVTPSCKELA DLMTRCMNYD PNQRPFFRAI MRDINKLEEQ
860 870 880 890 900
NPDIVSEKQP TTEVDPTHFE KRFLKRIRDL GEGHFGKVEL CRYDPEGDNT
910 920 930 940 950
GEQVAVKSLK PESGGNHIAD LKKEIEILRN LYHENIVKYK GICMEDGGNG
960 970 980 990 1000
IKLIMEFLPS GSLKEYLPKN KNKINLKQQL KYAIQICKGM DYLGSRQYVH
1010 1020 1030 1040 1050
RDLAARNVLV ESEHQVKIGD FGLTKAIETD KEYYTVKDDR DSPVFWYAPE
1060 1070 1080 1090 1100
CLIQCKFYIA SDVWSFGVTL HELLTYCDSD FSPMALFLKM IGPTHGQMTV
1110 1120 1130 1140 1150
TRLVKTLKEG KRLPCPPNCP DEVYQLMRKC WEFQPSNRTT FQNLIEGFEA

LLK
Length:1,153
Mass (Da):133,367
Last modified:October 1, 1996 - v1
Checksum:i0B9816AEA5B3868B
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti999 – 9991V → I(PubMed:2482828)Curated
Sequence conflicti999 – 9991V → I(PubMed:2466296)Curated
Sequence conflicti1068 – 10692VT → IP(PubMed:2482828)Curated
Sequence conflicti1068 – 10692VT → IP(PubMed:2466296)Curated

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S63728 mRNA. Translation: AAB27517.2.
M33425 mRNA. Translation: AAA40016.1.
UniGeneiMm.289657.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
S63728 mRNA. Translation: AAB27517.2 .
M33425 mRNA. Translation: AAA40016.1 .
UniGenei Mm.289657.

3D structure databases

ProteinModelPortali P52332.
SMRi P52332. Positions 515-1153.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

DIPi DIP-41965N.
IntActi P52332. 2 interactions.
MINTi MINT-188445.

Chemistry

BindingDBi P52332.
ChEMBLi CHEMBL2968.

PTM databases

PhosphoSitei P52332.

Proteomic databases

MaxQBi P52332.
PaxDbi P52332.
PRIDEi P52332.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Organism-specific databases

MGIi MGI:96628. Jak1.

Phylogenomic databases

eggNOGi COG0515.
HOGENOMi HOG000049158.
HOVERGENi HBG006195.
InParanoidi P52332.
PhylomeDBi P52332.

Enzyme and pathway databases

BRENDAi 2.7.10.2. 3474.
Reactomei REACT_198701. Interleukin-7 signaling.
SABIO-RK P52332.

Miscellaneous databases

ChiTaRSi JAK1. mouse.
PROi P52332.
SOURCEi Search...

Gene expression databases

CleanExi MM_JAK1.
Genevestigatori P52332.

Family and domain databases

Gene3Di 3.30.505.10. 1 hit.
InterProi IPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view ]
Pfami PF07714. Pkinase_Tyr. 2 hits.
[Graphical view ]
PIRSFi PIRSF000636. TyrPK_Jak. 1 hit.
PRINTSi PR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTi SM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view ]
SUPFAMi SSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEi PS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Molecular cloning of the murine JAK1 protein tyrosine kinase and its expression in the mouse central nervous system."
    Yang X., Chung D., Cepko C.L.
    J. Neurosci. 13:3006-3017(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. Lubec G., Sunyer B., Chen W.-Q.
    Submitted (JAN-2009) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 718-723, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: OF1.
    Tissue: Hippocampus.
  3. "The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family."
    Wilks A.F., Kurban R.R., Hovens C.M., Ralph S.J.
    Gene 85:67-74(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
  4. "Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction."
    Wilks A.F.
    Proc. Natl. Acad. Sci. U.S.A. 86:1603-1607(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
  5. "Fer is a downstream effector of insulin and mediates the activation of signal transducer and activator of transcription 3 in myogenic cells."
    Taler M., Shpungin S., Salem Y., Malovani H., Pasder O., Nir U.
    Mol. Endocrinol. 17:1580-1592(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH FER.

Entry informationi

Entry nameiJAK1_MOUSE
AccessioniPrimary (citable) accession number: P52332
Secondary accession number(s): Q62126
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: October 29, 2014
This is version 137 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. MGD cross-references
    Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
  2. Human and mouse protein kinases
    Human and mouse protein kinases: classification and index
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3