Reviewed, UniProtKB/Swiss-Prot P52332 (JAK1_MOUSE)

Names and origin

Protein names

Recommended name:
    Tyrosine-protein kinase JAK1
    EC=2.7.10.2
Alternative name(s):
    Janus kinase 1
      Short name=JAK-1

Gene names

Name:

Jak1

Organism

Mus musculus (Mouse)

Taxonomic identifier

10090 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Mus

Protein attributes

Sequence length	1153 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.
Catalytic activity	ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
Subunit structure	Interacts with IL31RA, JAKMIP1 and SHB By similarity.
Subcellular location	Endomembrane system; Peripheral membrane protein. Note: Wholly intracellular, possibly membrane associated.
Domain	The FERM domain mediates interaction with JAKMIP1 By similarity. Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.
Sequence similarities	Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily. Contains 1 FERM domain. Contains 1 protein kinase domain. Contains 1 SH2 domain.

Ontologies

Keywords
Cellular component	Membrane
Domain	Repeat SH2 domain
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase Tyrosine-protein kinase
PTM	Phosphoprotein
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	cytokine-mediated signaling pathway Inferred from direct assay. Source: MGI enzyme linked receptor protein signaling pathway Inferred from direct assay. Source: MGI peptidyl-tyrosine phosphorylation Inferred from direct assay. Source: MGI protein kinase cascade Inferred from direct assay. Source: MGI
Cellular component	cytoskeleton Inferred from electronic annotation. Source: InterPro extrinsic to membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW Janus kinase activity Inferred from electronic annotation. Source: InterPro growth hormone receptor binding Inferred from physical interaction. Source: UniProtKB non-membrane spanning protein tyrosine kinase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1153	1153	Tyrosine-protein kinase JAK1		PRO_0000088109
Regions
	Domain	34 – 420	387	FERM
	Domain	439 – 542	104	SH2
	Domain	582 – 854	273	Protein kinase 1
	Domain	874 – 1152	279	Protein kinase 2
	Nucleotide binding	880 – 888	9	ATP By similarity
Sites
	Active site	1002	1	Proton acceptor By similarity
	Binding site	907	1	ATP By similarity
Amino acid modifications
	Modified residue	1033	1	Phosphotyrosine; by autocatalysis By similarity
	Modified residue	1034	1	Phosphotyrosine By similarity
Experimental info
	Sequence conflict	999	1	V → I Ref.3
	Sequence conflict	999	1	V → I Ref.4
	Sequence conflict	1068 – 1069	2	VT → IP Ref.3
	Sequence conflict	1068 – 1069	2	VT → IP Ref.4

Sequences

	Sequence		Length	Mass (Da)
	P52332-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 0B9816AEA5B3868B	FASTA	1,153	133,367
	10 20 30 40 50 60 MQYLNIKEDC NAMAFCAKMR SFKKTEVKQV VPEPGVEVTF YLLDREPLRL GSGEYTAEEL 70 80 90 100 110 120 CIRAAQECSI SPLCHNLFAL YDESTKLWYA PNRIITVDDK TSLRLHYRMR FYFTNWHGTN 130 140 150 160 170 180 DNEQSVWRHS PKKQKNGYEK KRVPEATPLL DASSLEYLFA QGQYDLIKCL APIRDPKTEQ 190 200 210 220 230 240 DGHDIENECL GMAVLAISHY AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI 250 260 270 280 290 300 NNVFKDFLKE FNNKTICDSS VHDLKVKYLA TLETSTLTKH YGAEIFETSM LLISSENELS 310 320 330 340 350 360 RCHSNDSGNV LYEVMVTGNL GIQWRQKPNV VPVEKEKNKL KRKKLEYNKH KKDDERNKLR 370 380 390 400 410 420 EEWNNFSYFP EITHIVIKES VVSINKQDNK NMELKLSSRE EALSFVSLVD GYFRLTADAH 430 440 450 460 470 480 HYLCTDVAPP LIVHNIQNGC HGPICTEYAI NKLRQEGSEE GMYVLRWSCT DFDNILMTVT 490 500 510 520 530 540 CFEKSEVLGG QKQFKNFQIE VQKGRYSLHG SMDHFPSLRD LMNHLKKQIL RTDNISFVLK 550 560 570 580 590 600 RCCQPKPREI SNLLVATKKA QEWQPVYSMS QLSFDRILKK DIIQGEHLGR GTRTHIYSGT 610 620 630 640 650 660 LLDYKDEEGI AEEKKIKVIL KVLDPSHRDI SLAFFEAASM MRQVSHKHIV YLYGVCVRDV 670 680 690 700 710 720 ENIMVEEFVE GGPLDLFMHR KSDALTTPWK FKVAKQLASA LSYLEDKDLV HGNVCTKNLL 730 740 750 760 770 780 LAREGIDSDI GPFIKLSDPG IPVSVLTRQE CIERIPWIAP ECVEDSKNLS VAADKWSFGT 790 800 810 820 830 840 TLWEICYNGE IPLKDKTLIE KERFYESRCR PVTPSCKELA DLMTRCMNYD PNQRPFFRAI 850 860 870 880 890 900 MRDINKLEEQ NPDIVSEKQP TTEVDPTHFE KRFLKRIRDL GEGHFGKVEL CRYDPEGDNT 910 920 930 940 950 960 GEQVAVKSLK PESGGNHIAD LKKEIEILRN LYHENIVKYK GICMEDGGNG IKLIMEFLPS 970 980 990 1000 1010 1020 GSLKEYLPKN KNKINLKQQL KYAIQICKGM DYLGSRQYVH RDLAARNVLV ESEHQVKIGD 1030 1040 1050 1060 1070 1080 FGLTKAIETD KEYYTVKDDR DSPVFWYAPE CLIQCKFYIA SDVWSFGVTL HELLTYCDSD 1090 1100 1110 1120 1130 1140 FSPMALFLKM IGPTHGQMTV TRLVKTLKEG KRLPCPPNCP DEVYQLMRKC WEFQPSNRTT 1150 FQNLIEGFEA LLK

References

[1]	"Molecular cloning of the murine JAK1 protein tyrosine kinase and its expression in the mouse central nervous system." Yang X., Chung D., Cepko C.L. J. Neurosci. 13:3006-3017(1993) [PubMed: 8331382] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]	Lubec G., Sunyer B., Chen W.-Q. Submitted (JAN-2009) to UniProtKB Cited for: PROTEIN SEQUENCE OF 718-723, MASS SPECTROMETRY. Strain: OF1. Tissue: Hippocampus.
[3]	"The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family." Wilks A.F., Kurban R.R., Hovens C.M., Ralph S.J. Gene 85:67-74(1989) [PubMed: 2482828] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
[4]	"Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction." Wilks A.F. Proc. Natl. Acad. Sci. U.S.A. 86:1603-1607(1989) [PubMed: 2466296] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBLGenBankDDBJ	S63728 mRNA. Translation: AAB27517.2. M33425 mRNA. Translation: AAA40016.1.
IPI	IPI00126078.
UniGene	Mm.289657
3D structure databases
HSSP	HSSP built from PDB template 1JQH based on UniProtKB P08069.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52332. 1 interaction.
STRING	P52332.
PTM databases
PhosphoSite	P52332.
Proteomic databases
PRIDE	P52332.
Genome annotation databases
Ensembl	ENSMUST00000102781; ENSMUSP00000099842; ENSMUSG00000028530; Mus musculus. [Genome view]
Organism-specific databases
MGI	MGI:96628. Jak1.
Phylogenomic databases
HOGENOM	P52332.
HOVERGEN	P52332.
Enzyme and pathway databases
BRENDA	2.7.10.2. 244.
Gene expression databases
ArrayExpress	P52332.
Bgee	P52332.
CleanEx	MM_JAK1.
Genevestigator	P52332.
GermOnline	ENSMUSG00000028530. Mus musculus.
Family and domain databases
InterPro	IPR019749. Band_41_domain. IPR019747. FERM_CS. IPR000299. FERM_domain. IPR009127. JAK. IPR009128. JAK1. IPR000719. Prot_kinase_core. IPR017441. Protein_kinase_ATP_BS. IPR000980. SH2. IPR001245. Tyr_pkinase. IPR008266. Tyr_pkinase_AS. IPR016251. TyrPK_Jak. [Graphical view]
Pfam	PF07714. Pkinase_Tyr. 2 hits. [Graphical view]
PIRSF	PIRSF000636. TyrPK_Jak. 1 hit.
PRINTS	PR01823. JANUSKINASE. PR01824. JANUSKINASE1. PR00109. TYRKINASE.
ProDom	PD000001. Prot_kinase. 2 hits. PD000093. SH2. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00295. B41. 1 hit. SM00252. SH2. 1 hit. SM00219. TyrKc. 2 hits. [Graphical view]
PROSITE	PS00660. FERM_1. False negative. PS00661. FERM_2. False negative. PS50057. FERM_3. 1 hit. PS00107. PROTEIN_KINASE_ATP. 1 hit. PS50011. PROTEIN_KINASE_DOM. 2 hits. PS00109. PROTEIN_KINASE_TYR. 1 hit. PS50001. SH2. 1 hit. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

JAK1_MOUSE

Accession

Primary (citable) accession number: P52332
Secondary accession number(s): Q62126

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	October 13, 2009
This is version 91 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)