Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Basket 0
(max 400 entries)x

Your basket is currently empty.

Select item(s) and click on "Add to basket" to create your own collection here
(400 entries max)

P52332

- JAK1_MOUSE

UniProt

P52332 - JAK1_MOUSE

Protein

Tyrosine-protein kinase JAK1

Gene

Jak1

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli
    • BLAST
    • Align
    • Format
    • Add to basket
    • History
      Entry version 136 (01 Oct 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
    • Help video
    • Feedback
    • Comment

    Functioni

    Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

    Catalytic activityi

    ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei907 – 9071ATPPROSITE-ProRule annotation
    Active sitei1002 – 10021Proton acceptorPROSITE-ProRule annotation

    Regions

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Nucleotide bindingi880 – 8889ATPPROSITE-ProRule annotation

    GO - Molecular functioni

    1. ATP binding Source: UniProtKB-KW
    2. growth hormone receptor binding Source: BHF-UCL
    3. non-membrane spanning protein tyrosine kinase activity Source: UniProtKB-EC
    4. protein binding Source: MGI
    5. protein tyrosine kinase activity Source: MGI

    GO - Biological processi

    1. cytokine-mediated signaling pathway Source: MGI
    2. enzyme linked receptor protein signaling pathway Source: MGI
    3. interleukin-2-mediated signaling pathway Source: UniProtKB
    4. intracellular signal transduction Source: MGI
    5. peptidyl-tyrosine phosphorylation Source: MGI
    6. response to antibiotic Source: MGI

    Keywords - Molecular functioni

    Kinase, Transferase, Tyrosine-protein kinase

    Keywords - Ligandi

    ATP-binding, Nucleotide-binding

    Enzyme and pathway databases

    BRENDAi2.7.10.2. 3474.
    ReactomeiREACT_198701. Interleukin-7 signaling.
    SABIO-RKP52332.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
    Alternative name(s):
    Janus kinase 1
    Short name:
    JAK-1
    Gene namesi
    Name:Jak1
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Unplaced

    Organism-specific databases

    MGIiMGI:96628. Jak1.

    Subcellular locationi

    Endomembrane system; Peripheral membrane protein
    Note: Wholly intracellular, possibly membrane associated.

    GO - Cellular componenti

    1. cytoskeleton Source: InterPro
    2. cytosol Source: Reactome
    3. endomembrane system Source: UniProtKB-SubCell
    4. membrane Source: UniProtKB-KW

    Keywords - Cellular componenti

    Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 11531153Tyrosine-protein kinase JAK1PRO_0000088109Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei1 – 11N-acetylmethionineBy similarity
    Modified residuei3 – 31PhosphotyrosineBy similarity
    Modified residuei228 – 2281PhosphoserineBy similarity
    Modified residuei1033 – 10331Phosphotyrosine; by autocatalysisBy similarity
    Modified residuei1034 – 10341Phosphotyrosine; by autocatalysisBy similarity

    Post-translational modificationi

    Phosphorylated. Dephosphorylation of Tyr-1033 and Tyr-1034 by PTPN2 negatively regulates cytokine-mediated signaling By similarity.By similarity

    Keywords - PTMi

    Acetylation, Phosphoprotein

    Proteomic databases

    MaxQBiP52332.
    PaxDbiP52332.
    PRIDEiP52332.

    PTM databases

    PhosphoSiteiP52332.

    Expressioni

    Gene expression databases

    CleanExiMM_JAK1.
    GenevestigatoriP52332.

    Interactioni

    Subunit structurei

    Interacts with IL31RA, IFNAR2, JAKMIP1 and SHB By similarity. Interacts with FER.By similarity1 Publication

    Protein-protein interaction databases

    DIPiDIP-41965N.
    IntActiP52332. 2 interactions.
    MINTiMINT-188445.

    Structurei

    3D structure databases

    ProteinModelPortaliP52332.
    SMRiP52332. Positions 34-1153.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini34 – 420387FERMPROSITE-ProRule annotationAdd
    BLAST
    Domaini439 – 542104SH2PROSITE-ProRule annotationAdd
    BLAST
    Domaini582 – 854273Protein kinase 1PROSITE-ProRule annotationAdd
    BLAST
    Domaini874 – 1152279Protein kinase 2PROSITE-ProRule annotationAdd
    BLAST

    Domaini

    The FERM domain mediates interaction with JAKMIP1.By similarity
    Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.

    Sequence similaritiesi

    Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation
    Contains 1 FERM domain.PROSITE-ProRule annotation
    Contains 2 protein kinase domains.PROSITE-ProRule annotation
    Contains 1 SH2 domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Repeat, SH2 domain

    Phylogenomic databases

    eggNOGiCOG0515.
    HOGENOMiHOG000049158.
    HOVERGENiHBG006195.
    InParanoidiP52332.
    PhylomeDBiP52332.

    Family and domain databases

    Gene3Di3.30.505.10. 1 hit.
    InterProiIPR019749. Band_41_domain.
    IPR019748. FERM_central.
    IPR000299. FERM_domain.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
    IPR020776. Tyr_kinase_non-rcpt_Jak1.
    [Graphical view]
    PfamiPF07714. Pkinase_Tyr. 2 hits.
    [Graphical view]
    PIRSFiPIRSF000636. TyrPK_Jak. 1 hit.
    PRINTSiPR01823. JANUSKINASE.
    PR01824. JANUSKINASE1.
    PR00109. TYRKINASE.
    SMARTiSM00295. B41. 1 hit.
    SM00252. SH2. 1 hit.
    SM00219. TyrKc. 2 hits.
    [Graphical view]
    SUPFAMiSSF47031. SSF47031. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEiPS50057. FERM_3. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 2 hits.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    P52332-1 [UniParc]FASTAAdd to Basket

    « Hide

    MQYLNIKEDC NAMAFCAKMR SFKKTEVKQV VPEPGVEVTF YLLDREPLRL     50
    GSGEYTAEEL CIRAAQECSI SPLCHNLFAL YDESTKLWYA PNRIITVDDK 100
    TSLRLHYRMR FYFTNWHGTN DNEQSVWRHS PKKQKNGYEK KRVPEATPLL 150
    DASSLEYLFA QGQYDLIKCL APIRDPKTEQ DGHDIENECL GMAVLAISHY 200
    AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI NNVFKDFLKE 250
    FNNKTICDSS VHDLKVKYLA TLETSTLTKH YGAEIFETSM LLISSENELS 300
    RCHSNDSGNV LYEVMVTGNL GIQWRQKPNV VPVEKEKNKL KRKKLEYNKH 350
    KKDDERNKLR EEWNNFSYFP EITHIVIKES VVSINKQDNK NMELKLSSRE 400
    EALSFVSLVD GYFRLTADAH HYLCTDVAPP LIVHNIQNGC HGPICTEYAI 450
    NKLRQEGSEE GMYVLRWSCT DFDNILMTVT CFEKSEVLGG QKQFKNFQIE 500
    VQKGRYSLHG SMDHFPSLRD LMNHLKKQIL RTDNISFVLK RCCQPKPREI 550
    SNLLVATKKA QEWQPVYSMS QLSFDRILKK DIIQGEHLGR GTRTHIYSGT 600
    LLDYKDEEGI AEEKKIKVIL KVLDPSHRDI SLAFFEAASM MRQVSHKHIV 650
    YLYGVCVRDV ENIMVEEFVE GGPLDLFMHR KSDALTTPWK FKVAKQLASA 700
    LSYLEDKDLV HGNVCTKNLL LAREGIDSDI GPFIKLSDPG IPVSVLTRQE 750
    CIERIPWIAP ECVEDSKNLS VAADKWSFGT TLWEICYNGE IPLKDKTLIE 800
    KERFYESRCR PVTPSCKELA DLMTRCMNYD PNQRPFFRAI MRDINKLEEQ 850
    NPDIVSEKQP TTEVDPTHFE KRFLKRIRDL GEGHFGKVEL CRYDPEGDNT 900
    GEQVAVKSLK PESGGNHIAD LKKEIEILRN LYHENIVKYK GICMEDGGNG 950
    IKLIMEFLPS GSLKEYLPKN KNKINLKQQL KYAIQICKGM DYLGSRQYVH 1000
    RDLAARNVLV ESEHQVKIGD FGLTKAIETD KEYYTVKDDR DSPVFWYAPE 1050
    CLIQCKFYIA SDVWSFGVTL HELLTYCDSD FSPMALFLKM IGPTHGQMTV 1100
    TRLVKTLKEG KRLPCPPNCP DEVYQLMRKC WEFQPSNRTT FQNLIEGFEA 1150
    LLK 1153
    Length:1,153
    Mass (Da):133,367
    Last modified:October 1, 1996 - v1
    Checksum:i0B9816AEA5B3868B
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti999 – 9991V → I(PubMed:2482828)Curated
    Sequence conflicti999 – 9991V → I(PubMed:2466296)Curated
    Sequence conflicti1068 – 10692VT → IP(PubMed:2482828)Curated
    Sequence conflicti1068 – 10692VT → IP(PubMed:2466296)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S63728 mRNA. Translation: AAB27517.2.
    M33425 mRNA. Translation: AAA40016.1.
    UniGeneiMm.289657.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    S63728 mRNA. Translation: AAB27517.2 .
    M33425 mRNA. Translation: AAA40016.1 .
    UniGenei Mm.289657.

    3D structure databases

    ProteinModelPortali P52332.
    SMRi P52332. Positions 34-1153.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    DIPi DIP-41965N.
    IntActi P52332. 2 interactions.
    MINTi MINT-188445.

    Chemistry

    BindingDBi P52332.
    ChEMBLi CHEMBL2968.

    PTM databases

    PhosphoSitei P52332.

    Proteomic databases

    MaxQBi P52332.
    PaxDbi P52332.
    PRIDEi P52332.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Organism-specific databases

    MGIi MGI:96628. Jak1.

    Phylogenomic databases

    eggNOGi COG0515.
    HOGENOMi HOG000049158.
    HOVERGENi HBG006195.
    InParanoidi P52332.
    PhylomeDBi P52332.

    Enzyme and pathway databases

    BRENDAi 2.7.10.2. 3474.
    Reactomei REACT_198701. Interleukin-7 signaling.
    SABIO-RK P52332.

    Miscellaneous databases

    ChiTaRSi JAK1. mouse.
    PROi P52332.
    SOURCEi Search...

    Gene expression databases

    CleanExi MM_JAK1.
    Genevestigatori P52332.

    Family and domain databases

    Gene3Di 3.30.505.10. 1 hit.
    InterProi IPR019749. Band_41_domain.
    IPR019748. FERM_central.
    IPR000299. FERM_domain.
    IPR011009. Kinase-like_dom.
    IPR000719. Prot_kinase_dom.
    IPR017441. Protein_kinase_ATP_BS.
    IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
    IPR000980. SH2.
    IPR008266. Tyr_kinase_AS.
    IPR020635. Tyr_kinase_cat_dom.
    IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
    IPR020776. Tyr_kinase_non-rcpt_Jak1.
    [Graphical view ]
    Pfami PF07714. Pkinase_Tyr. 2 hits.
    [Graphical view ]
    PIRSFi PIRSF000636. TyrPK_Jak. 1 hit.
    PRINTSi PR01823. JANUSKINASE.
    PR01824. JANUSKINASE1.
    PR00109. TYRKINASE.
    SMARTi SM00295. B41. 1 hit.
    SM00252. SH2. 1 hit.
    SM00219. TyrKc. 2 hits.
    [Graphical view ]
    SUPFAMi SSF47031. SSF47031. 1 hit.
    SSF55550. SSF55550. 1 hit.
    SSF56112. SSF56112. 2 hits.
    PROSITEi PS50057. FERM_3. 1 hit.
    PS00107. PROTEIN_KINASE_ATP. 1 hit.
    PS50011. PROTEIN_KINASE_DOM. 2 hits.
    PS00109. PROTEIN_KINASE_TYR. 1 hit.
    PS50001. SH2. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "Molecular cloning of the murine JAK1 protein tyrosine kinase and its expression in the mouse central nervous system."
      Yang X., Chung D., Cepko C.L.
      J. Neurosci. 13:3006-3017(1993) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    2. Lubec G., Sunyer B., Chen W.-Q.
      Submitted (JAN-2009) to UniProtKB
      Cited for: PROTEIN SEQUENCE OF 718-723, IDENTIFICATION BY MASS SPECTROMETRY.
      Strain: OF1.
      Tissue: Hippocampus.
    3. "The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family."
      Wilks A.F., Kurban R.R., Hovens C.M., Ralph S.J.
      Gene 85:67-74(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
    4. "Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction."
      Wilks A.F.
      Proc. Natl. Acad. Sci. U.S.A. 86:1603-1607(1989) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
    5. "Fer is a downstream effector of insulin and mediates the activation of signal transducer and activator of transcription 3 in myogenic cells."
      Taler M., Shpungin S., Salem Y., Malovani H., Pasder O., Nir U.
      Mol. Endocrinol. 17:1580-1592(2003) [PubMed] [Europe PMC] [Abstract]
      Cited for: INTERACTION WITH FER.

    Entry informationi

    Entry nameiJAK1_MOUSE
    AccessioniPrimary (citable) accession number: P52332
    Secondary accession number(s): Q62126
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: October 1, 2014
    This is version 136 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Direct protein sequencing, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. Human and mouse protein kinases
      Human and mouse protein kinases: classification and index
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3