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Protein

Tyrosine-protein kinase JAK1

Gene

Jak1

Organism
Mus musculus (Mouse)
Status
Reviewed-Annotation score: -Experimental evidence at protein leveli

Functioni

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.By similarity

Catalytic activityi

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.PROSITE-ProRule annotationBy similarity

Cofactori

Mg2+By similarityNote: Mn2+ was used in the in vitro kinase assay but Mg2+ is likely to be the in vivo cofactor.By similarity

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei907ATPPROSITE-ProRule annotation1
Active sitei1002Proton acceptorPROSITE-ProRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi880 – 888ATPPROSITE-ProRule annotation9

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionKinase, Transferase, Tyrosine-protein kinase
LigandATP-binding, Magnesium, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BRENDAi2.7.10.2 3474
SABIO-RKP52332

Names & Taxonomyi

Protein namesi
Recommended name:
Tyrosine-protein kinase JAK1 (EC:2.7.10.2)
Alternative name(s):
Janus kinase 1
Short name:
JAK-1
Gene namesi
Name:Jak1
OrganismiMus musculus (Mouse)
Taxonomic identifieri10090 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaMyomorphaMuroideaMuridaeMurinaeMusMus
Proteomesi
  • UP000000589 Componenti: Unplaced

Organism-specific databases

MGIiMGI:96628 Jak1

Subcellular locationi

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionGraphics by Christian Stolte; Source: COMPARTMENTS

Keywords - Cellular componenti

Membrane

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL2968

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000881091 – 1153Tyrosine-protein kinase JAK1Add BLAST1153

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei1N-acetylmethionineBy similarity1
Modified residuei3PhosphotyrosineBy similarity1
Modified residuei228PhosphoserineBy similarity1
Modified residuei1033Phosphotyrosine; by autocatalysisBy similarity1
Modified residuei1034Phosphotyrosine; by autocatalysisBy similarity1

Post-translational modificationi

Ubiquitinated by RNF125; leading to its degradation by the proteasome.By similarity
Autophosphorylated. Phosphorylated on tyrosine residues in response to interferon gamma signaling. Dephosphorylation of Tyr-1034 and Tyr-1034 by PTPN2 negatively regulates cytokine-mediated signaling.By similarity

Keywords - PTMi

Acetylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP52332
MaxQBiP52332
PaxDbiP52332
PeptideAtlasiP52332
PRIDEiP52332

PTM databases

iPTMnetiP52332
PhosphoSitePlusiP52332
SwissPalmiP52332

Expressioni

Gene expression databases

CleanExiMM_JAK1

Interactioni

Subunit structurei

Interacts with IL31RA, IFNAR2, IFNGR1, JAKMIP1 and SHB (By similarity). Interacts with FER (PubMed:12738762).By similarity1 Publication

GO - Molecular functioni

Protein-protein interaction databases

CORUMiP52332
DIPiDIP-41965N
IntActiP52332, 5 interactors
MINTiP52332
STRINGi10090.ENSMUSP00000099842

Chemistry databases

BindingDBiP52332

Structurei

3D structure databases

ProteinModelPortaliP52332
SMRiP52332
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini34 – 420FERMPROSITE-ProRule annotationAdd BLAST387
Domaini439 – 542SH2PROSITE-ProRule annotationAdd BLAST104
Domaini582 – 854Protein kinase 1PROSITE-ProRule annotationAdd BLAST273
Domaini874 – 1152Protein kinase 2PROSITE-ProRule annotationAdd BLAST279

Domaini

The FERM domain mediates interaction with JAKMIP1.By similarity
Possesses two phosphotransferase domains. The second one probably contains the catalytic domain, while the presence of slight differences suggest a different role for domain 1.By similarity

Sequence similaritiesi

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, SH2 domain

Phylogenomic databases

eggNOGiKOG0197 Eukaryota
COG0515 LUCA
HOGENOMiHOG000049158
HOVERGENiHBG006195
InParanoidiP52332
PhylomeDBiP52332

Family and domain databases

CDDicd14473 FERM_B-lobe, 1 hit
Gene3Di3.30.505.10, 1 hit
InterProiView protein in InterPro
IPR019749 Band_41_domain
IPR035963 FERM_2
IPR019748 FERM_central
IPR000299 FERM_domain
IPR011009 Kinase-like_dom_sf
IPR000719 Prot_kinase_dom
IPR017441 Protein_kinase_ATP_BS
IPR001245 Ser-Thr/Tyr_kinase_cat_dom
IPR000980 SH2
IPR036860 SH2_dom_sf
IPR008266 Tyr_kinase_AS
IPR020635 Tyr_kinase_cat_dom
IPR016251 Tyr_kinase_non-rcpt_Jak/Tyk2
IPR020776 Tyr_kinase_non-rcpt_Jak1
PfamiView protein in Pfam
PF07714 Pkinase_Tyr, 2 hits
PIRSFiPIRSF000636 TyrPK_Jak, 1 hit
PRINTSiPR01823 JANUSKINASE
PR01824 JANUSKINASE1
PR00109 TYRKINASE
SMARTiView protein in SMART
SM00295 B41, 1 hit
SM00252 SH2, 1 hit
SM00219 TyrKc, 2 hits
SUPFAMiSSF47031 SSF47031, 1 hit
SSF55550 SSF55550, 1 hit
SSF56112 SSF56112, 2 hits
PROSITEiView protein in PROSITE
PS50057 FERM_3, 1 hit
PS00107 PROTEIN_KINASE_ATP, 1 hit
PS50011 PROTEIN_KINASE_DOM, 2 hits
PS00109 PROTEIN_KINASE_TYR, 1 hit
PS50001 SH2, 1 hit

Sequencei

Sequence statusi: Complete.

P52332-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQYLNIKEDC NAMAFCAKMR SFKKTEVKQV VPEPGVEVTF YLLDREPLRL
60 70 80 90 100
GSGEYTAEEL CIRAAQECSI SPLCHNLFAL YDESTKLWYA PNRIITVDDK
110 120 130 140 150
TSLRLHYRMR FYFTNWHGTN DNEQSVWRHS PKKQKNGYEK KRVPEATPLL
160 170 180 190 200
DASSLEYLFA QGQYDLIKCL APIRDPKTEQ DGHDIENECL GMAVLAISHY
210 220 230 240 250
AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI NNVFKDFLKE
260 270 280 290 300
FNNKTICDSS VHDLKVKYLA TLETSTLTKH YGAEIFETSM LLISSENELS
310 320 330 340 350
RCHSNDSGNV LYEVMVTGNL GIQWRQKPNV VPVEKEKNKL KRKKLEYNKH
360 370 380 390 400
KKDDERNKLR EEWNNFSYFP EITHIVIKES VVSINKQDNK NMELKLSSRE
410 420 430 440 450
EALSFVSLVD GYFRLTADAH HYLCTDVAPP LIVHNIQNGC HGPICTEYAI
460 470 480 490 500
NKLRQEGSEE GMYVLRWSCT DFDNILMTVT CFEKSEVLGG QKQFKNFQIE
510 520 530 540 550
VQKGRYSLHG SMDHFPSLRD LMNHLKKQIL RTDNISFVLK RCCQPKPREI
560 570 580 590 600
SNLLVATKKA QEWQPVYSMS QLSFDRILKK DIIQGEHLGR GTRTHIYSGT
610 620 630 640 650
LLDYKDEEGI AEEKKIKVIL KVLDPSHRDI SLAFFEAASM MRQVSHKHIV
660 670 680 690 700
YLYGVCVRDV ENIMVEEFVE GGPLDLFMHR KSDALTTPWK FKVAKQLASA
710 720 730 740 750
LSYLEDKDLV HGNVCTKNLL LAREGIDSDI GPFIKLSDPG IPVSVLTRQE
760 770 780 790 800
CIERIPWIAP ECVEDSKNLS VAADKWSFGT TLWEICYNGE IPLKDKTLIE
810 820 830 840 850
KERFYESRCR PVTPSCKELA DLMTRCMNYD PNQRPFFRAI MRDINKLEEQ
860 870 880 890 900
NPDIVSEKQP TTEVDPTHFE KRFLKRIRDL GEGHFGKVEL CRYDPEGDNT
910 920 930 940 950
GEQVAVKSLK PESGGNHIAD LKKEIEILRN LYHENIVKYK GICMEDGGNG
960 970 980 990 1000
IKLIMEFLPS GSLKEYLPKN KNKINLKQQL KYAIQICKGM DYLGSRQYVH
1010 1020 1030 1040 1050
RDLAARNVLV ESEHQVKIGD FGLTKAIETD KEYYTVKDDR DSPVFWYAPE
1060 1070 1080 1090 1100
CLIQCKFYIA SDVWSFGVTL HELLTYCDSD FSPMALFLKM IGPTHGQMTV
1110 1120 1130 1140 1150
TRLVKTLKEG KRLPCPPNCP DEVYQLMRKC WEFQPSNRTT FQNLIEGFEA

LLK
Length:1,153
Mass (Da):133,367
Last modified:October 1, 1996 - v1
Checksum:i0B9816AEA5B3868B
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti999V → I (PubMed:2482828).Curated1
Sequence conflicti999V → I (PubMed:2466296).Curated1
Sequence conflicti1068 – 1069VT → IP (PubMed:2482828).Curated2
Sequence conflicti1068 – 1069VT → IP (PubMed:2466296).Curated2

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
S63728 mRNA Translation: AAB27517.2
M33425 mRNA Translation: AAA40016.1
UniGeneiMm.289657

Similar proteinsi

Entry informationi

Entry nameiJAK1_MOUSE
AccessioniPrimary (citable) accession number: P52332
Secondary accession number(s): Q62126
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 23, 2018
This is version 169 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

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