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P52332 (JAK1_MOUSE) Reviewed, UniProtKB/Swiss-Prot

Last modified June 11, 2014. Version 134. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Tyrosine-protein kinase JAK1

EC=2.7.10.2
Alternative name(s):
Janus kinase 1
Short name=JAK-1
Gene names
Name:Jak1
OrganismMus musculus (Mouse) [Reference proteome]
Taxonomic identifier10090 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus

Protein attributes

Sequence length1153 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Tyrosine kinase of the non-receptor type, involved in the IFN-alpha/beta/gamma signal pathway. Kinase partner for the interleukin (IL)-2 receptor.

Catalytic activity

ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.

Subunit structure

Interacts with IL31RA, IFNAR2, JAKMIP1 and SHB By similarity. Interacts with FER. Ref.5

Subcellular location

Endomembrane system; Peripheral membrane protein. Note: Wholly intracellular, possibly membrane associated.

Domain

The FERM domain mediates interaction with JAKMIP1 By similarity.

Possesses two phosphotransferase domains. The second one probably contains the catalytic domain By similarity, while the presence of slight differences suggest a different role for domain 1.

Post-translational modification

Phosphorylated. Dephosphorylation of Tyr-1033 and Tyr-1034 by PTPN2 negatively regulates cytokine-mediated signaling By similarity.

Sequence similarities

Belongs to the protein kinase superfamily. Tyr protein kinase family. JAK subfamily.

Contains 1 FERM domain.

Contains 2 protein kinase domains.

Contains 1 SH2 domain.

Ontologies

Keywords
   Cellular componentMembrane
   DomainRepeat
SH2 domain
   LigandATP-binding
Nucleotide-binding
   Molecular functionKinase
Transferase
Tyrosine-protein kinase
   PTMAcetylation
Phosphoprotein
   Technical termComplete proteome
Direct protein sequencing
Reference proteome
Gene Ontology (GO)
   Biological_processcytokine-mediated signaling pathway

Inferred from direct assay PubMed 8022486. Source: MGI

enzyme linked receptor protein signaling pathway

Inferred from direct assay PubMed 8022486. Source: MGI

interleukin-2-mediated signaling pathway

Inferred from sequence or structural similarity. Source: UniProtKB

intracellular signal transduction

Inferred from direct assay PubMed 8022486. Source: MGI

peptidyl-tyrosine phosphorylation

Inferred from direct assay PubMed 10872802. Source: MGI

response to antibiotic

Inferred from sequence orthology PubMed 16280321. Source: MGI

   Cellular_componentcytoskeleton

Inferred from electronic annotation. Source: InterPro

cytosol

Traceable author statement. Source: Reactome

endomembrane system

Inferred from electronic annotation. Source: UniProtKB-SubCell

membrane

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

growth hormone receptor binding

Inferred from physical interaction PubMed 10502458. Source: BHF-UCL

non-membrane spanning protein tyrosine kinase activity

Inferred from electronic annotation. Source: UniProtKB-EC

protein binding

Inferred from physical interaction Ref.5PubMed 15659653. Source: MGI

protein tyrosine kinase activity

Inferred from direct assay PubMed 10872802. Source: MGI

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 11531153Tyrosine-protein kinase JAK1
PRO_0000088109

Regions

Domain34 – 420387FERM
Domain439 – 542104SH2
Domain582 – 854273Protein kinase 1
Domain874 – 1152279Protein kinase 2
Nucleotide binding880 – 8889ATP By similarity

Sites

Active site10021Proton acceptor By similarity
Binding site9071ATP By similarity

Amino acid modifications

Modified residue11N-acetylmethionine By similarity
Modified residue31Phosphotyrosine By similarity
Modified residue2281Phosphoserine By similarity
Modified residue10331Phosphotyrosine; by autocatalysis By similarity
Modified residue10341Phosphotyrosine; by autocatalysis By similarity

Experimental info

Sequence conflict9991V → I Ref.3
Sequence conflict9991V → I Ref.4
Sequence conflict1068 – 10692VT → IP Ref.3
Sequence conflict1068 – 10692VT → IP Ref.4

Sequences

Sequence LengthMass (Da)Tools
P52332 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: 0B9816AEA5B3868B

FASTA1,153133,367
        10         20         30         40         50         60 
MQYLNIKEDC NAMAFCAKMR SFKKTEVKQV VPEPGVEVTF YLLDREPLRL GSGEYTAEEL 

        70         80         90        100        110        120 
CIRAAQECSI SPLCHNLFAL YDESTKLWYA PNRIITVDDK TSLRLHYRMR FYFTNWHGTN 

       130        140        150        160        170        180 
DNEQSVWRHS PKKQKNGYEK KRVPEATPLL DASSLEYLFA QGQYDLIKCL APIRDPKTEQ 

       190        200        210        220        230        240 
DGHDIENECL GMAVLAISHY AMMKKMQLPE LPKDISYKRY IPETLNKSIR QRNLLTRMRI 

       250        260        270        280        290        300 
NNVFKDFLKE FNNKTICDSS VHDLKVKYLA TLETSTLTKH YGAEIFETSM LLISSENELS 

       310        320        330        340        350        360 
RCHSNDSGNV LYEVMVTGNL GIQWRQKPNV VPVEKEKNKL KRKKLEYNKH KKDDERNKLR 

       370        380        390        400        410        420 
EEWNNFSYFP EITHIVIKES VVSINKQDNK NMELKLSSRE EALSFVSLVD GYFRLTADAH 

       430        440        450        460        470        480 
HYLCTDVAPP LIVHNIQNGC HGPICTEYAI NKLRQEGSEE GMYVLRWSCT DFDNILMTVT 

       490        500        510        520        530        540 
CFEKSEVLGG QKQFKNFQIE VQKGRYSLHG SMDHFPSLRD LMNHLKKQIL RTDNISFVLK 

       550        560        570        580        590        600 
RCCQPKPREI SNLLVATKKA QEWQPVYSMS QLSFDRILKK DIIQGEHLGR GTRTHIYSGT 

       610        620        630        640        650        660 
LLDYKDEEGI AEEKKIKVIL KVLDPSHRDI SLAFFEAASM MRQVSHKHIV YLYGVCVRDV 

       670        680        690        700        710        720 
ENIMVEEFVE GGPLDLFMHR KSDALTTPWK FKVAKQLASA LSYLEDKDLV HGNVCTKNLL 

       730        740        750        760        770        780 
LAREGIDSDI GPFIKLSDPG IPVSVLTRQE CIERIPWIAP ECVEDSKNLS VAADKWSFGT 

       790        800        810        820        830        840 
TLWEICYNGE IPLKDKTLIE KERFYESRCR PVTPSCKELA DLMTRCMNYD PNQRPFFRAI 

       850        860        870        880        890        900 
MRDINKLEEQ NPDIVSEKQP TTEVDPTHFE KRFLKRIRDL GEGHFGKVEL CRYDPEGDNT 

       910        920        930        940        950        960 
GEQVAVKSLK PESGGNHIAD LKKEIEILRN LYHENIVKYK GICMEDGGNG IKLIMEFLPS 

       970        980        990       1000       1010       1020 
GSLKEYLPKN KNKINLKQQL KYAIQICKGM DYLGSRQYVH RDLAARNVLV ESEHQVKIGD 

      1030       1040       1050       1060       1070       1080 
FGLTKAIETD KEYYTVKDDR DSPVFWYAPE CLIQCKFYIA SDVWSFGVTL HELLTYCDSD 

      1090       1100       1110       1120       1130       1140 
FSPMALFLKM IGPTHGQMTV TRLVKTLKEG KRLPCPPNCP DEVYQLMRKC WEFQPSNRTT 

      1150 
FQNLIEGFEA LLK 

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References

[1]"Molecular cloning of the murine JAK1 protein tyrosine kinase and its expression in the mouse central nervous system."
Yang X., Chung D., Cepko C.L.
J. Neurosci. 13:3006-3017(1993) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA].
[2]Lubec G., Sunyer B., Chen W.-Q.
Submitted (JAN-2009) to UniProtKB
Cited for: PROTEIN SEQUENCE OF 718-723, IDENTIFICATION BY MASS SPECTROMETRY.
Strain: OF1.
Tissue: Hippocampus.
[3]"The application of the polymerase chain reaction to cloning members of the protein tyrosine kinase family."
Wilks A.F., Kurban R.R., Hovens C.M., Ralph S.J.
Gene 85:67-74(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
[4]"Two putative protein-tyrosine kinases identified by application of the polymerase chain reaction."
Wilks A.F.
Proc. Natl. Acad. Sci. U.S.A. 86:1603-1607(1989) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA] OF 999-1069.
[5]"Fer is a downstream effector of insulin and mediates the activation of signal transducer and activator of transcription 3 in myogenic cells."
Taler M., Shpungin S., Salem Y., Malovani H., Pasder O., Nir U.
Mol. Endocrinol. 17:1580-1592(2003) [PubMed] [Europe PMC] [Abstract]
Cited for: INTERACTION WITH FER.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
S63728 mRNA. Translation: AAB27517.2.
M33425 mRNA. Translation: AAA40016.1.
UniGeneMm.289657.

3D structure databases

ProteinModelPortalP52332.
SMRP52332. Positions 34-1153.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

DIPDIP-41965N.
IntActP52332. 2 interactions.
MINTMINT-188445.

Chemistry

BindingDBP52332.
ChEMBLCHEMBL2968.

PTM databases

PhosphoSiteP52332.

Proteomic databases

MaxQBP52332.
PaxDbP52332.
PRIDEP52332.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Organism-specific databases

MGIMGI:96628. Jak1.

Phylogenomic databases

eggNOGCOG0515.
HOGENOMHOG000049158.
HOVERGENHBG006195.
InParanoidP52332.
PhylomeDBP52332.

Enzyme and pathway databases

BRENDA2.7.10.2. 3474.
SABIO-RKP52332.

Gene expression databases

CleanExMM_JAK1.
GenevestigatorP52332.

Family and domain databases

Gene3D3.30.505.10. 1 hit.
InterProIPR019749. Band_41_domain.
IPR019748. FERM_central.
IPR000299. FERM_domain.
IPR011009. Kinase-like_dom.
IPR000719. Prot_kinase_dom.
IPR017441. Protein_kinase_ATP_BS.
IPR001245. Ser-Thr/Tyr_kinase_cat_dom.
IPR000980. SH2.
IPR008266. Tyr_kinase_AS.
IPR020635. Tyr_kinase_cat_dom.
IPR016251. Tyr_kinase_non-rcpt_Jak/Tyk2.
IPR020776. Tyr_kinase_non-rcpt_Jak1.
[Graphical view]
PfamPF07714. Pkinase_Tyr. 2 hits.
[Graphical view]
PIRSFPIRSF000636. TyrPK_Jak. 1 hit.
PRINTSPR01823. JANUSKINASE.
PR01824. JANUSKINASE1.
PR00109. TYRKINASE.
SMARTSM00295. B41. 1 hit.
SM00252. SH2. 1 hit.
SM00219. TyrKc. 2 hits.
[Graphical view]
SUPFAMSSF47031. SSF47031. 1 hit.
SSF55550. SSF55550. 1 hit.
SSF56112. SSF56112. 2 hits.
PROSITEPS50057. FERM_3. 1 hit.
PS00107. PROTEIN_KINASE_ATP. 1 hit.
PS50011. PROTEIN_KINASE_DOM. 2 hits.
PS00109. PROTEIN_KINASE_TYR. 1 hit.
PS50001. SH2. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

ChiTaRSJAK1. mouse.
PROP52332.
SOURCESearch...

Entry information

Entry nameJAK1_MOUSE
AccessionPrimary (citable) accession number: P52332
Secondary accession number(s): Q62126
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: June 11, 2014
This is version 134 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Human and mouse protein kinases

Human and mouse protein kinases: classification and index

MGD cross-references

Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot