ID   MTX2_XANOR              Reviewed;         424 AA.
AC   P52311; Q5H5A9;
DT   01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT   29-MAR-2005, sequence version 2.
DT   24-JAN-2024, entry version 125.
DE   RecName: Full=Type II methyltransferase M.XorII {ECO:0000303|PubMed:12654995};
DE            Short=M.XorII {ECO:0000303|PubMed:8078464};
DE            EC=2.1.1.37;
DE   AltName: Full=Cytosine-specific methyltransferase XorII;
DE   AltName: Full=Modification methylase XorII;
GN   Name=xorIIM; OrderedLocusNames=XOO0607;
OS   Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85).
OC   Bacteria; Pseudomonadota; Gammaproteobacteria; Xanthomonadales;
OC   Xanthomonadaceae; Xanthomonas.
OX   NCBI_TaxID=291331;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND FUNCTION.
RC   STRAIN=JW89011;
RX   PubMed=8078464; DOI=10.1007/bf00286690;
RA   Choi S.H., Leach J.E.;
RT   "Identification of the XorII methyltransferase gene and a vsr homolog from
RT   Xanthomonas oryzae pv. oryzae.";
RL   Mol. Gen. Genet. 244:383-390(1994).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=KACC10331 / KXO85;
RX   PubMed=15673718; DOI=10.1093/nar/gki206;
RA   Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S.,
RA   Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H.,
RA   Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S.,
RA   Go S.-J.;
RT   "The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the
RT   bacterial blight pathogen of rice.";
RL   Nucleic Acids Res. 33:577-586(2005).
RN   [3]
RP   NOMENCLATURE.
RX   PubMed=12654995; DOI=10.1093/nar/gkg274;
RA   Roberts R.J., Belfort M., Bestor T., Bhagwat A.S., Bickle T.A.,
RA   Bitinaite J., Blumenthal R.M., Degtyarev S.K., Dryden D.T., Dybvig K.,
RA   Firman K., Gromova E.S., Gumport R.I., Halford S.E., Hattman S.,
RA   Heitman J., Hornby D.P., Janulaitis A., Jeltsch A., Josephsen J., Kiss A.,
RA   Klaenhammer T.R., Kobayashi I., Kong H., Krueger D.H., Lacks S.,
RA   Marinus M.G., Miyahara M., Morgan R.D., Murray N.E., Nagaraja V.,
RA   Piekarowicz A., Pingoud A., Raleigh E., Rao D.N., Reich N., Repin V.E.,
RA   Selker E.U., Shaw P.C., Stein D.C., Stoddard B.L., Szybalski W.,
RA   Trautner T.A., Van Etten J.L., Vitor J.M., Wilson G.G., Xu S.Y.;
RT   "A nomenclature for restriction enzymes, DNA methyltransferases, homing
RT   endonucleases and their genes.";
RL   Nucleic Acids Res. 31:1805-1812(2003).
CC   -!- FUNCTION: A methylase that recognizes the double-stranded sequence 5'-
CC       CGATCG-3', methylates C-? on both strands and protects the DNA from
CC       cleavage by the XorII endonuclease. {ECO:0000303|PubMed:12654995,
CC       ECO:0000305|PubMed:8078464}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 2'-deoxycytidine in DNA + S-adenosyl-L-methionine = a 5-
CC         methyl-2'-deoxycytidine in DNA + H(+) + S-adenosyl-L-homocysteine;
CC         Xref=Rhea:RHEA:13681, Rhea:RHEA-COMP:11369, Rhea:RHEA-COMP:11370,
CC         ChEBI:CHEBI:15378, ChEBI:CHEBI:57856, ChEBI:CHEBI:59789,
CC         ChEBI:CHEBI:85452, ChEBI:CHEBI:85454; EC=2.1.1.37;
CC         Evidence={ECO:0000255|PROSITE-ProRule:PRU10018};
CC   -!- SIMILARITY: Belongs to the class I-like SAM-binding methyltransferase
CC       superfamily. C5-methyltransferase family. {ECO:0000255|PROSITE-
CC       ProRule:PRU01016}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAW73861.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; U06424; AAA50432.1; -; Genomic_DNA.
DR   EMBL; AE013598; AAW73861.1; ALT_INIT; Genomic_DNA.
DR   PIR; S46293; S46293.
DR   AlphaFoldDB; P52311; -.
DR   SMR; P52311; -.
DR   STRING; 291331.XOO0607; -.
DR   REBASE; 10811; M.XorKI.
DR   REBASE; 203186; M.Bam1267ORF544P.
DR   REBASE; 3534; M.XorII.
DR   KEGG; xoo:XOO0607; -.
DR   HOGENOM; CLU_006958_2_4_6; -.
DR   PRO; PR:P52311; -.
DR   Proteomes; UP000006735; Chromosome.
DR   GO; GO:0003886; F:DNA (cytosine-5-)-methyltransferase activity; IEA:UniProtKB-EC.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0009307; P:DNA restriction-modification system; IEA:UniProtKB-KW.
DR   CDD; cd00315; Cyt_C5_DNA_methylase; 1.
DR   Gene3D; 3.90.120.10; DNA Methylase, subunit A, domain 2; 1.
DR   Gene3D; 3.40.50.150; Vaccinia Virus protein VP39; 1.
DR   InterPro; IPR018117; C5_DNA_meth_AS.
DR   InterPro; IPR001525; C5_MeTfrase.
DR   InterPro; IPR031303; C5_meth_CS.
DR   InterPro; IPR029063; SAM-dependent_MTases_sf.
DR   NCBIfam; TIGR00675; dcm; 1.
DR   PANTHER; PTHR10629; CYTOSINE-SPECIFIC METHYLTRANSFERASE; 1.
DR   PANTHER; PTHR10629:SF52; DNA (CYTOSINE-5)-METHYLTRANSFERASE 1; 1.
DR   Pfam; PF00145; DNA_methylase; 1.
DR   PRINTS; PR00105; C5METTRFRASE.
DR   SUPFAM; SSF53335; S-adenosyl-L-methionine-dependent methyltransferases; 1.
DR   PROSITE; PS00094; C5_MTASE_1; 1.
DR   PROSITE; PS00095; C5_MTASE_2; 1.
DR   PROSITE; PS51679; SAM_MT_C5; 1.
PE   3: Inferred from homology;
KW   DNA-binding; Methyltransferase; Reference proteome; Restriction system;
KW   S-adenosyl-L-methionine; Transferase.
FT   CHAIN           1..424
FT                   /note="Type II methyltransferase M.XorII"
FT                   /id="PRO_0000087910"
FT   DOMAIN          4..367
FT                   /note="SAM-dependent MTase C5-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01016"
FT   REGION          404..424
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COMPBIAS        407..424
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   ACT_SITE        83
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU01016,
FT                   ECO:0000255|PROSITE-ProRule:PRU10018"
FT   CONFLICT        60..61
FT                   /note="GD -> RS (in Ref. 1; AAA50432)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   424 AA;  46900 MW;  EBC3FD08DBACADEC CRC64;
     MTRPIGIDLF AGAGGLSLGF EQAGFDLVAA VDIDPIHCAA HKFNFPKCAT VCKSVVDVTG
     DELRRIAGIG KRDIDIVIGG APCQGFSLIG KRALDDSRNQ LVHHYVRVVM ELKPKYFVFE
     NVKGLTVGKH RQFLKEVIEA FQNGGYDVVT DYRVLNAADY GVPQDRRRLI LMGARKGLPL
     PAYPEPTGRT TVGDAIGDIP DAESFPELWE RDWVKAKYGK PSTYAAYLRG KKDDPTDFGY
     RRQFDPMLLT GSLLTDHTEL SRQRFAAIAP DDVEPVSRFK KLALNGICNT LRAGTASDRG
     AFTSPRPIHP TVPRVITVRE AARLHSYPDW FRFHATKWHG FRQIGNSVPP LLARAVGGQI
     MKALRKKPEK PSEMLALGDQ SLIGISMTDA AAMFGVSSTV IARRSRPVDR PAPRRHEERE
     LVTA
//