P52311 (MTX2_XANOR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase XorII
Short name=M.XorII
EC=2.1.1.37

Alternative name(s):
Cytosine-specific methyltransferase XorII

Gene names

Name:	xorIIM
Ordered Locus Names:	XOO0607

Organism

Xanthomonas oryzae pv. oryzae (strain KACC10331 / KXO85) [Complete proteome] [HAMAP]

Taxonomic identifier

291331 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas › Xanthomonas oryzae › Xanthomonas oryzae pv. oryzae

Protein attributes

Sequence length	424 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence CGATCG, causes specific methylation on C-? on both strands and protects the DNA from cleavage by the XorII endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
Sequence similarities	Belongs to the C5-methyltransferase family.
Sequence caution	The sequence AAW73861.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological_process	C-5 methylation of cytosine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	DNA (cytosine-5-)-methyltransferase activity Inferred from electronic annotation. Source: EC DNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 424	424	Modification methylase XorII		PRO_0000087910
Sites
	Active site	83	1	By similarity
Experimental info
	Sequence conflict	60 – 61	2	GD → RS in AAA50432. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52311 [UniParc]. Last modified March 29, 2005. Version 2. Checksum: EBC3FD08DBACADEC	FASTA	424	46,900
	10 20 30 40 50 60 MTRPIGIDLF AGAGGLSLGF EQAGFDLVAA VDIDPIHCAA HKFNFPKCAT VCKSVVDVTG 70 80 90 100 110 120 DELRRIAGIG KRDIDIVIGG APCQGFSLIG KRALDDSRNQ LVHHYVRVVM ELKPKYFVFE 130 140 150 160 170 180 NVKGLTVGKH RQFLKEVIEA FQNGGYDVVT DYRVLNAADY GVPQDRRRLI LMGARKGLPL 190 200 210 220 230 240 PAYPEPTGRT TVGDAIGDIP DAESFPELWE RDWVKAKYGK PSTYAAYLRG KKDDPTDFGY 250 260 270 280 290 300 RRQFDPMLLT GSLLTDHTEL SRQRFAAIAP DDVEPVSRFK KLALNGICNT LRAGTASDRG 310 320 330 340 350 360 AFTSPRPIHP TVPRVITVRE AARLHSYPDW FRFHATKWHG FRQIGNSVPP LLARAVGGQI 370 380 390 400 410 420 MKALRKKPEK PSEMLALGDQ SLIGISMTDA AAMFGVSSTV IARRSRPVDR PAPRRHEERE LVTA

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Identification of the XorII methyltransferase gene and a vsr homolog from Xanthomonas oryzae pv. oryzae." Choi S.H., Leach J.E. Mol. Gen. Genet. 244:383-390(1994) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: JW89011.
[2]	"The genome sequence of Xanthomonas oryzae pathovar oryzae KACC10331, the bacterial blight pathogen of rice." Lee B.-M., Park Y.-J., Park D.-S., Kang H.-W., Kim J.-G., Song E.-S., Park I.-C., Yoon U.-H., Hahn J.-H., Koo B.-S., Lee G.-B., Kim H., Park H.-S., Yoon K.-O., Kim J.-H., Jung C.-H., Koh N.-H., Seo J.-S., Go S.-J. Nucleic Acids Res. 33:577-586(2005) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: KACC10331 / KXO85.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U06424 Genomic DNA. Translation: AAA50432.1. AE013598 Genomic DNA. Translation: AAW73861.1. Different initiation.
PIR	S46293.
RefSeq	YP_199246.1. NC_006834.1.
3D structure databases
ProteinModelPortal	P52311.
ModBase	Search...
Protein-protein interaction databases
STRING	291331.XOO0607.
Protein family/group databases
REBASE	3534. M.XorII.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
EnsemblBacteria	AAW73861; AAW73861; XOO0607.
GeneID	3264300.
KEGG	xoo:XOO0607.
PATRIC	24100062. VBIXanOry111333_0681.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0270.
HOGENOM	HOG000225505.
KO	K00558.
ProtClustDB	CLSK880091.
Family and domain databases
InterPro	IPR018117. C5_DNA_meth_AS. IPR001525. C5_MeTfrase. [Graphical view]
PANTHER	PTHR10629. PTHR10629. 1 hit.
Pfam	PF00145. DNA_methylase. 1 hit. [Graphical view]
PRINTS	PR00105. C5METTRFRASE.
TIGRFAMs	TIGR00675. dcm. 1 hit.
PROSITE	PS00094. C5_MTASE_1. 1 hit. PS00095. C5_MTASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTX2_XANOR

Accession

Primary (citable) accession number: P52311
Secondary accession number(s): Q5H5A9

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 29, 2005
Last modified:	May 1, 2013
This is version 78 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program