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P52307

- 5NTD_RHIMP

UniProt

P52307 - 5NTD_RHIMP

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Protein

Protein 5NUC

Gene
N/A
Organism
Rhipicephalus microplus (Cattle tick) (Boophilus microplus)
Status
Reviewed - Annotation score: 5 out of 5- Experimental evidence at protein leveli

Functioni

Degradation of external UDP-glucose to uridine monophosphate and glucose-1-phosphate, which can then be used by the cell.

Catalytic activityi

UDP-sugar + H2O = UMP + alpha-D-aldose 1-phosphate.
A 5'-ribonucleotide + H2O = a ribonucleoside + phosphate.

Cofactori

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi26 – 261Zinc 1By similarity
Metal bindingi28 – 281Zinc 1By similarity
Metal bindingi79 – 791Zinc 1By similarity
Metal bindingi79 – 791Zinc 2By similarity
Metal bindingi111 – 1111Zinc 2By similarity
Sitei112 – 1121Transition state stabilizerBy similarity
Sitei115 – 1151Transition state stabilizerBy similarity
Metal bindingi212 – 2121Zinc 2By similarity
Metal bindingi235 – 2351Zinc 2By similarity
Binding sitei350 – 3501SubstrateBy similarity
Binding sitei387 – 3871SubstrateBy similarity
Binding sitei392 – 3921SubstrateBy similarity
Binding sitei415 – 4151SubstrateBy similarity

GO - Molecular functioni

  1. 5'-nucleotidase activity Source: UniProtKB-EC
  2. metal ion binding Source: UniProtKB-KW
  3. nucleotide binding Source: UniProtKB-KW
  4. UDP-sugar diphosphatase activity Source: UniProtKB-EC

GO - Biological processi

  1. nucleotide catabolic process Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

SABIO-RKP52307.

Names & Taxonomyi

Protein namesi
Recommended name:
Protein 5NUC
Including the following 2 domains:
UDP-sugar hydrolase (EC:3.6.1.45)
Alternative name(s):
UDP-sugar diphosphatase
UDP-sugar pyrophosphatase
5'-nucleotidase (EC:3.1.3.5)
Short name:
5'-NT
OrganismiRhipicephalus microplus (Cattle tick) (Boophilus microplus)
Taxonomic identifieri6941 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaChelicerataArachnidaAcariParasitiformesIxodidaIxodoideaIxodidaeRhipicephalinaeRhipicephalusBoophilus

Subcellular locationi

GO - Cellular componenti

  1. anchored component of membrane Source: UniProtKB-KW
  2. plasma membrane Source: UniProtKB-KW
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Signal peptidei‹1 – 14›141 PublicationAdd
BLAST
Chaini15 – 555541Protein 5NUCPRO_0000000021Add
BLAST
Propeptidei556 – 58025Removed in mature formSequence AnalysisPRO_0000000022Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Disulfide bondi41 ↔ 51By similarity
Glycosylationi172 – 1721N-linked (GlcNAc...)Curated
Glycosylationi285 – 2851N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi349 ↔ 354By similarity
Glycosylationi423 – 4231N-linked (GlcNAc...)Sequence Analysis
Disulfide bondi478 ↔ 481By similarity
Glycosylationi536 – 5361N-linked (GlcNAc...)Sequence Analysis
Lipidationi555 – 5551GPI-anchor amidated asparagineSequence Analysis

Post-translational modificationi

Glycosylated.

Keywords - PTMi

Disulfide bond, Glycoprotein, GPI-anchor, Lipoprotein

Expressioni

Tissue specificityi

Gut, ovaries and salivary glands.

Interactioni

Subunit structurei

Homodimer.

Structurei

3D structure databases

ProteinModelPortaliP52307.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni502 – 5087Substrate bindingBy similarity

Sequence similaritiesi

Belongs to the 5'-nucleotidase family.Curated

Keywords - Domaini

Signal

Family and domain databases

Gene3Di3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProiIPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view]
PANTHERiPTHR11575. PTHR11575. 1 hit.
PfamiPF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view]
PRINTSiPR01607. APYRASEFAMLY.
SUPFAMiSSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEiPS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Fragment.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52307-1 [UniParc]FASTAAdd to Basket

« Hide

        10         20         30         40         50
GRLLACLLLP GLAATDFTAT VLHTNDVHGR FEQITASGTR CTKQAAEAQQ
60 70 80 90 100
CVGGIARQKT VVSQAAASGA NVLFLNAGDY YQGSIWYYVL GAPIVAEAVN
110 120 130 140 150
YLAHDAMALG NHEFDRGAEG LVPLLTESRV PIVGCNVDFS EEPTLKPLQP
160 170 180 190 200
KPSVVVERAG IKIGLIGYTT MNTTYLSSPG KVRFTDEAEC IQREAQRLRR
210 220 230 240 250
EEGVQVIIAV GHSGVPRDLE ICERVPEVSL VVGGHTHTFL YSGPTENGRV
260 270 280 290 300
SGDKPQGPYP IVVDRAADSR CLVVQDFYMG KYMGNISITW NQRGEVVRWS
310 320 330 340 350
GQPVLLDRSI PEDPDGIALL DRYRDRVAQS QASVVAESKV LLDGDKNRCR
360 370 380 390 400
LDECNLGNLV MDAFLKKMAS LPSPQASWTR VAAVIANAGG IRASIDEQST
410 420 430 440 450
GGRITFEDVV NVLPFGNTLV EMNVTGAQLK GLLEHGVHRH DVKGYVPPAE
460 470 480 490 500
LVLAAGLRVV YDIQREPYDR VVEAHILCTE CRVPRYEPLE YARQYRIAAL
510 520 530 540 550
SYIVHGGDNF DFSFVKPKDM YDTGFQDAEI VMKYMNSTSP ITTALDGRVT
560 570 580
FLKTNQASDA CLNLASPFLV LLVLVVFYHL
Length:580
Mass (Da):63,460
Last modified:January 11, 2001 - v2
Checksum:i588EEF2014071AB7
GO

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Non-terminal residuei1 – 11
Sequence conflicti15 – 151T → K AA sequence (PubMed:8387372)Curated
Sequence conflicti37 – 393SGT → HXG AA sequence (PubMed:8387372)Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80634 mRNA. Translation: AAB38963.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U80634 mRNA. Translation: AAB38963.1 .

3D structure databases

ProteinModelPortali P52307.
ModBasei Search...
MobiDBi Search...

Protocols and materials databases

Structural Biology Knowledgebase Search...

Enzyme and pathway databases

SABIO-RK P52307.

Family and domain databases

Gene3Di 3.60.21.10. 1 hit.
3.90.780.10. 1 hit.
InterProi IPR008334. 5'-Nucleotdase_C.
IPR006146. 5'-Nucleotdase_CS.
IPR006179. 5_nucleotidase/apyrase.
IPR004843. Calcineurin-like_PHP_apaH.
IPR029052. Metallo-depent_PP-like.
[Graphical view ]
PANTHERi PTHR11575. PTHR11575. 1 hit.
Pfami PF02872. 5_nucleotid_C. 1 hit.
PF00149. Metallophos. 1 hit.
[Graphical view ]
PRINTSi PR01607. APYRASEFAMLY.
SUPFAMi SSF55816. SSF55816. 1 hit.
SSF56300. SSF56300. 1 hit.
PROSITEi PS00786. 5_NUCLEOTIDASE_2. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. "Cloning and expression of ecto 5-nucleotidase from the cattle tick Boophilus microplus."
    Liyou N., Hamilton S., Elvin C., Willadsen P.
    Insect Mol. Biol. 8:257-266(1999) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
  2. "The nucleotidase of Boophilus microplus and its relationship to enzymes from the rat and Escherichia coli."
    Willadsen P., Riding G.A., Jarmey J., Atkins A.
    Insect Biochem. Mol. Biol. 23:291-295(1993) [PubMed] [Europe PMC] [Abstract]
    Cited for: PROTEIN SEQUENCE OF 15-40 AND 162-180.

Entry informationi

Entry namei5NTD_RHIMP
AccessioniPrimary (citable) accession number: P52307
Secondary accession number(s): P90696
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 11, 2001
Last modified: November 26, 2014
This is version 93 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3