Reviewed, UniProtKB/Swiss-Prot P52306 (GDS1_HUMAN)

Names and origin

Protein names

Recommended name:
    Rap1 GTPase-GDP dissociation stimulator 1
Alternative name(s):
    SMG GDS protein
    SMG P21 stimulatory GDP/GTP exchange protein
    Exchange factor smgGDS

Gene names

Name:

RAP1GDS1

Organism

Homo sapiens (Human) [Complete proteome]

Taxonomic identifier

9606 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Homo

Protein attributes

Sequence length	607 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Stimulates GDP/GTP exchange reaction of a group of small GTP-binding proteins (G proteins) including Rap1a/Rap1b, RhoA, RhoB and KRas, by stimulating the dissociation of GDP from and the subsequent binding of GTP to each small G protein.
Sequence similarities	Contains 5 ARM repeats.

Ontologies

Keywords
Coding sequence diversity	Alternative splicing Polymorphism
Domain	Repeat
Molecular function	GTPase activation
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Molecular function	GTPase activator activity Ref.1 Non-traceable author statement. Source: UniProtKB binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 (identifier: P52306-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: P52306-2)

The sequence of this isoform differs from the canonical sequence as follows:
     122-170: Missing.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 607	607	Rap1 GTPase-GDP dissociation stimulator 1		PRO_0000056759
Regions
	Repeat	77 – 118	42	ARM 1
	Repeat	169 – 211	43	ARM 2
	Repeat	346 – 390	45	ARM 3
	Repeat	391 – 431	41	ARM 4
	Repeat	479 – 519	41	ARM 5
Amino acid modifications
	Modified residue	230	1	N6-acetyllysine Ref.8
	Modified residue	421	1	N6-acetyllysine Ref.8
Natural variations
	Alternative sequence	122 – 170	49	Missing in isoform 2.		VSP_001658
	Natural variant	314	1	K → E: dbSNP rs34392334.		VAR_049158
Experimental info
	Sequence conflict	376	1	T → P in AAF43211. Ref.3
	Sequence conflict	394	1	N → D in CAA45067. Ref.1
	Sequence conflict	394	1	N → D in AAF32290. Ref.2
	Sequence conflict	593	1	A → R in CAA45067. Ref.1
	Sequence conflict	593	1	A → R in AAF32290. Ref.2

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 4, 2008. Version 3. Checksum: ED0F359DFA3F0510	FASTA	607	66,317
	10 20 30 40 50 60 MDNLSDTLKK LKITAVDKTE DSLEGCLDCL LQALAQNNTE TSEKIQASGI LQLFASLLTP 70 80 90 100 110 120 QSSCKAKVAN IIAEVAKNEF MRIPCVDAGL ISPLVQLLNS KDQEVLLQTG RALGNICYDS 130 140 150 160 170 180 HEGRSAVDQA GGAQIVIDHL RSLCSITDPA NEKLLTVFCG MLMNYSNEND SLQAQLINMG 190 200 210 220 230 240 VIPTLVKLLG IHCQNAALTE MCLVAFGNLA ELESSKEQFA STNIAEELVK LFKKQIEHDK 250 260 270 280 290 300 REMIFEVLAP LAENDAIKLQ LVEAGLVECL LEIVQQKVDS DKEDDITELK TGSDLMVLLL 310 320 330 340 350 360 LGDESMQKLF EGGKGSVFQR VLSWIPSNNH QLQLAGALAI ANFARNDANC IHMVDNGIVE 370 380 390 400 410 420 KLMDLLDRHV EDGNVTVQHA ALSALRNLAI PVINKAKMLS AGVTEAVLKF LKSEMPPVQF 430 440 450 460 470 480 KLLGTLRMLI DAQAEAAEQL GKNVKLVERL VEWCEAKDHA GVMGESNRLL SALIRHSKSK 490 500 510 520 530 540 DVIKTIVQSG GIKHLVTMAT SEHVIMQNEA LVALALIAAL ELGTAEKDLE SAKLVQILHR 550 560 570 580 590 600 LLADERSAPE IKYNSMVLIC ALMGSECLHK EVQDLAFLDV VSKLRSHENK SVAQQASLTE QRLTVES
	Isoform 2. Checksum: 7DCE981DA08F4629	FASTA	558	61,037
	10 20 30 40 50 60 MDNLSDTLKK LKITAVDKTE DSLEGCLDCL LQALAQNNTE TSEKIQASGI LQLFASLLTP 70 80 90 100 110 120 QSSCKAKVAN IIAEVAKNEF MRIPCVDAGL ISPLVQLLNS KDQEVLLQTG RALGNICYDS 130 140 150 160 170 180 HSLQAQLINM GVIPTLVKLL GIHCQNAALT EMCLVAFGNL AELESSKEQF ASTNIAEELV 190 200 210 220 230 240 KLFKKQIEHD KREMIFEVLA PLAENDAIKL QLVEAGLVEC LLEIVQQKVD SDKEDDITEL 250 260 270 280 290 300 KTGSDLMVLL LLGDESMQKL FEGGKGSVFQ RVLSWIPSNN HQLQLAGALA IANFARNDAN 310 320 330 340 350 360 CIHMVDNGIV EKLMDLLDRH VEDGNVTVQH AALSALRNLA IPVINKAKML SAGVTEAVLK 370 380 390 400 410 420 FLKSEMPPVQ FKLLGTLRML IDAQAEAAEQ LGKNVKLVER LVEWCEAKDH AGVMGESNRL 430 440 450 460 470 480 LSALIRHSKS KDVIKTIVQS GGIKHLVTMA TSEHVIMQNE ALVALALIAA LELGTAEKDL 490 500 510 520 530 540 ESAKLVQILH RLLADERSAP EIKYNSMVLI CALMGSECLH KEVQDLAFLD VVSKLRSHEN 550 KSVAQQASLT EQRLTVES

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Molecular cloning of the human cDNA for a stimulatory GDP/GTP exchange protein for c-Ki-ras p21 and smg p21." Kikuchi A., Kaibuchi K., Hori Y., Nonaka H., Sakoda T., Kawamura M., Mizuno T., Takai Y. Oncogene 7:289-293(1992) [PubMed: 1549351] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 2). Tissue: Brain.
[2]	"SmgGDS displays differential binding and exchange activity towards different Ras isoforms." Vikis H.G., Stewart S., Guan K.L. Oncogene 21:2425-2432(2002) [PubMed: 11948427] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1).
[3]	"The major isoform of RAP1GDS1 mRNA in peripheral blood mononuclear cells encodes an isoform of smgGDS with 12 armadillo repeats." Hussey D.J., Albanese N.O., Dobrovic A. Submitted (FEB-2000) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [MRNA] (ISOFORM 1). Tissue: Peripheral blood.
[4]	"Generation and annotation of the DNA sequences of human chromosomes 2 and 4." Hillier L.W., Graves T.A., Fulton R.S., Fulton L.A., Pepin K.H., Minx P., Wagner-McPherson C., Layman D., Wylie K., Sekhon M., Becker M.C., Fewell G.A., Delehaunty K.D., Miner T.L., Nash W.E., Kremitzki C., Oddy L., Du H. , Sun H., Bradshaw-Cordum H., Ali J., Carter J., Cordes M., Harris A., Isak A., van Brunt A., Nguyen C., Du F., Courtney L., Kalicki J., Ozersky P., Abbott S., Armstrong J., Belter E.A., Caruso L., Cedroni M., Cotton M., Davidson T., Desai A., Elliott G., Erb T., Fronick C., Gaige T., Haakenson W., Haglund K., Holmes A., Harkins R., Kim K., Kruchowski S.S., Strong C.M., Grewal N., Goyea E., Hou S., Levy A., Martinka S., Mead K., McLellan M.D., Meyer R., Randall-Maher J., Tomlinson C., Dauphin-Kohlberg S., Kozlowicz-Reilly A., Shah N., Swearengen-Shahid S., Snider J., Strong J.T., Thompson J., Yoakum M., Leonard S., Pearman C., Trani L., Radionenko M., Waligorski J.E., Wang C., Rock S.M., Tin-Wollam A.-M., Maupin R., Latreille P., Wendl M.C., Yang S.-P., Pohl C., Wallis J.W., Spieth J., Bieri T.A., Berkowicz N., Nelson J.O., Osborne J., Ding L., Meyer R., Sabo A., Shotland Y., Sinha P., Wohldmann P.E., Cook L.L., Hickenbotham M.T., Eldred J., Williams D., Jones T.A., She X., Ciccarelli F.D., Izaurralde E., Taylor J., Schmutz J., Myers R.M., Cox D.R., Huang X., McPherson J.D., Mardis E.R., Clifton S.W., Warren W.C., Chinwalla A.T., Eddy S.R., Marra M.A., Ovcharenko I., Furey T.S., Miller W., Eichler E.E., Bork P., Suyama M., Torrents D., Waterston R.H., Wilson R.K. Nature 434:724-731(2005) [PubMed: 15815621] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[5]	Mural R.J., Istrail S., Sutton G.G., Florea L., Halpern A.L., Mobarry C.M., Lippert R., Walenz B., Shatkay H., Dew I., Miller J.R., Flanigan M.J., Edwards N.J., Bolanos R., Fasulo D., Halldorsson B.V., Hannenhalli S., Turner R. , Yooseph S., Lu F., Nusskern D.R., Shue B.C., Zheng X.H., Zhong F., Delcher A.L., Huson D.H., Kravitz S.A., Mouchard L., Reinert K., Remington K.A., Clark A.G., Waterman M.S., Eichler E.E., Adams M.D., Hunkapiller M.W., Myers E.W., Venter J.C. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
[6]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed: 15489334] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
[7]	Colinge J., Superti-Furga G., Bennett K.L. Submitted (OCT-2008) to UniProtKB Cited for: IDENTIFICATION [LARGE SCALE ANALYSIS], MASS SPECTROMETRY.
[8]	"Lysine acetylation targets protein complexes and co-regulates major cellular functions." Choudhary C., Kumar C., Gnad F., Nielsen M.L., Rehman M., Walther T., Olsen J.V., Mann M. Science 325:834-840(2009) [PubMed: 19608861] [Abstract] Cited for: ACETYLATION [LARGE SCALE ANALYSIS] AT LYS-230 AND LYS-421, MASS SPECTROMETRY.
+	Additional computationally mapped references.

Web resources

Atlas of Genetics and Cytogenetics in Oncology and Haematology

Cross-references

Sequence databases
EMBL GenBank DDBJ	X63465 mRNA. Translation: CAA45067.1. AF215923 mRNA. Translation: AAF32290.1. AF237413 mRNA. Translation: AAF43211.1. AC019077 Genomic DNA. No translation available. AC058823 Genomic DNA. No translation available. CH471057 Genomic DNA. Translation: EAX06071.1. BC098334 mRNA. Translation: AAH98334.1. BC099708 mRNA. Translation: AAH99708.1.
IPI	IPI00607591. IPI00856100.
PIR	I37456.
RefSeq	NP_001093896.1. NP_001093897.1. NP_001093899.1. NP_001093900.1.
UniGene	Hs.132858
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	P52306.
PTM databases
PhosphoSite	P52306.
Proteomic databases
PRIDE	P52306.
Genome annotation databases
Ensembl	ENST00000408927; ENSP00000386153; ENSG00000138698; Homo sapiens. [Genome view]
GeneID	5910.
KEGG	hsa:5910.
Organism-specific databases
CTD	5910.
GeneCards	GC04P099428.
H-InvDB	HIX0004387.
HGNC	HGNC:9859. RAP1GDS1.
HPA	HPA019060.
MIM	179502. gene.
PharmGKB	PA34221.
GenAtlas	Search...
Phylogenomic databases
eggNOG	prNOG17879.
HOVERGEN	P52306.
Gene expression databases
ArrayExpress	P52306.
Bgee	P52306.
CleanEx	HS_RAP1GDS1.
Genevestigator	P52306.
GermOnline	ENSG00000138698. Homo sapiens.
Family and domain databases
InterPro	IPR011989. ARM-like. IPR016024. ARM-type_fold. IPR000225. Armadillo. [Graphical view]
Gene3D	G3DSA:1.25.10.10. ARM-like. 2 hits.
Pfam	PF00514. Arm. 4 hits. [Graphical view]
SMART	SM00185. ARM. 5 hits. [Graphical view]
PROSITE	PS50176. ARM_REPEAT. 2 hits. [Graphical view]
ProtoNet	Search...
Other Resources
SOURCE	Search...

Entry information

Entry name

GDS1_HUMAN

Accession

Primary (citable) accession number: P52306
Secondary accession number(s): Q4KMV2, Q9NYM2, Q9NZA8

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	November 4, 2008
Last modified:	January 19, 2010
This is version 74 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)

Disclaimer

Any medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.