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P52303 (AP1B1_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 93. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
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to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
AP-1 complex subunit beta-1
Alternative name(s):
Adapter-related protein complex 1 subunit beta-1
Adaptor protein complex AP-1 subunit beta-1
Beta-1-adaptin
Beta-adaptin 1
Clathrin assembly protein complex 1 beta large chain
Golgi adaptor HA1/AP1 adaptin beta subunit
Gene names
Name:Ap1b1
Synonyms:Adtb1
OrganismRattus norvegicus (Rat)
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length949 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

Subunit structure

Adaptor protein complex 1 (AP-1) is an heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3). Ref.3

Subcellular location

Golgi apparatus. Cytoplasmic vesicle › clathrin-coated vesicle membrane; Peripheral membrane protein; Cytoplasmic side. Note: Component of the coat surrounding the cytoplasmic face of coated vesicles located at the Golgi complex.

Post-translational modification

The N-terminus is blocked.

Sequence similarities

Belongs to the adaptor complexes large subunit family.

Caution

Was originally (Ref.1) thought to be part of the complex AP-2.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform A (identifier: P52303-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform B (identifier: P52303-2)

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 949949AP-1 complex subunit beta-1
PRO_0000193740

Regions

Compositional bias576 – 728153Pro-rich (stalk region)

Amino acid modifications

Modified residue3181N6-acetyllysine By similarity
Modified residue9001Phosphotyrosine By similarity

Natural variations

Alternative sequence667 – 6737Missing in isoform B.
VSP_000164

Secondary structure

1 .............................................................................. 949
Helix Strand Turn

Details...

Sequences

Sequence LengthMass (Da)Tools
Isoform A [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: CC5AE54E8AACCD44

FASTA949104,589
        10         20         30         40         50         60 
MTDSKYFTTT KKGEIFELKA ELNSDKKEKK KEAVKKVIAS MTVGKDVSAL FPDVVNCMQT 

        70         80         90        100        110        120 
DNLELKKLVY LYLMNYAKSQ PDMAIMAVNT FVKDCEDPNP LIRALAVRTM GCIRVDKITE 

       130        140        150        160        170        180 
YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD INAQLVEDQG FLDTLKDLIS DSNPMVVANR 

       190        200        210        220        230        240 
VAALSEIAES HPSSNLLDLK AQSINKLLTA LNECTEWAQI FILDCLGNYM PKDDREAQSI 

       250        260        270        280        290        300 
CERVTPRLSH ANSAVVLSAV KVLMKFMEML SKDLDYYATL LKKLAPPLVT LLSAEPEPQY 

       310        320        330        340        350        360 
VPLRNINLIV QKRPEILKHE MKVFFVKYND PIYVKLEKLD IMIRLASQAN IAQVLAELKE 

       370        380        390        400        410        420 
YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ TKVNYVVQEA IVVIKDIFRK 

       430        440        450        460        470        480 
YPNKYESVIA TLCENLDSLD EPEARAAMIW IVGEYAERID NADELLESFL DGFHDESTQV 

       490        500        510        520        530        540 
QLQLLTAIVK LFLKKPTETQ ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVAAKEV 

       550        560        570        580        590        600 
VLAEKPLISE ETDLIEPTLL DELICYIGTL ASVYHKPPNA FVEGGRGVVH KSLPPRTASS 

       610        620        630        640        650        660 
ESTESPEAAP AGAPASDQPD VIPAQGDLLG DLLNLDLGPP VSGPPLAASS VQMGAVDLLG 

       670        680        690        700        710        720 
GGLDSLMGDE PEGIGDSNFG APPASVAAAA PARLGAPVSS GLSDLFDLTS GVGTLSGSYV 

       730        740        750        760        770        780 
APKAVWLPAM KAKGLEISGT FTRQVGSISM DLQLTNKALQ VMTDFAIQFN RNSFGLAPAA 

       790        800        810        820        830        840 
PLQVHAPLSP NQTVEISLPL NTVGSVMKME PLNNLQVAVK NNIDVFYFST LYPLHVLFVE 

       850        860        870        880        890        900 
DGKMDRQMFL ATWKDIPNEN EAQFQIRDCP LNTEAASSKL QSSNIFTVAK RTVEGQDMLY 

       910        920        930        940 
QSLKLTNGIW VLAELRIQPG NPSFTLSLKC RAPEVSQHDI QAYETILKN

« Hide

Isoform B [UniParc].

Checksum: 69F7E7D2234C7BC1
Show »

FASTA942103,873

References

[1]"Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2."
Kirchhausen T., Nathanson K.L., Matsui W., Vaisberg A., Chow E.P., Burne C., Keen J.H., Davis A.E.
Proc. Natl. Acad. Sci. U.S.A. 86:2612-2616(1989) [PubMed: 2495531] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
Tissue: Brain.
[2]"Characterization of a new member of the human beta-adaptin gene family from chromosome 22q12, a candidate meningioma gene."
Peyrard M., Fransson I., Xie Y.-G., Han F.-Y., Ruttledge M.H., Swahn S., Collins J.E., Dunham I., Collins V.P., Dumanski J.P.
Hum. Mol. Genet. 3:1393-1399(1994) [PubMed: 7987321] [Abstract]
Cited for: CHARACTERIZATION OF ISOFORMS A AND B.
[3]"Crystal structure of the clathrin adaptor protein 1 core."
Heldwein E.E., Macia E., Wang J., Yin H.L., Kirchhausen T., Harrison S.C.
Proc. Natl. Acad. Sci. U.S.A. 101:14108-14113(2004) [PubMed: 15377783] [Abstract]
Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 1-584, SUBUNIT.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		M77245 mRNA. Translation: AAA40807.1.
IPIIPI00201713.
IPI00781585.
PIRB32105.
RefSeqNP_058973.1. NM_017277.1.
UniGeneRn.37383.

3D structure databases

PDBe
RCSB PDB
PDBj
EntryMethodResolution (Å)ChainPositionsPDBsum
1W63X-ray4.00B/D/F/H/J/L1-584[»]
ProteinModelPortalP52303.
SMRP52303. Positions 2-583, 717-949.
ModBaseSearch...

Protein-protein interaction databases

IntActP52303. 3 interactions.
MINTMINT-1344522.
STRINGP52303.

PTM databases

PhosphoSiteP52303.

Proteomic databases

PRIDEP52303.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID29663.
KEGGrno:29663.
UCSCNM_017277. rat.

Organism-specific databases

CTD162.
RGD2064. Ap1b1.

Phylogenomic databases

eggNOGroNOG10896.
GeneTreeENSGT00530000063138.
HOVERGENHBG050515.
PhylomeDBP52303.

Gene expression databases

ArrayExpressP52303.
GenevestigatorP52303.
GermOnlineENSRNOG00000008786. Rattus norvegicus.

Family and domain databases

InterProIPR016342. AP_complex_bsu.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013037. Clathrin_b-adaptin_app_Ig-like.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
Gene3DG3DSA:1.25.10.10. ARM-like. 1 hit.
G3DSA:2.60.40.1150. Clathrin_b-adaptin_app_Ig-like. 1 hit.
KOK12392.
PfamPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFPIRSF002291. AP_complex_beta. 1 hit.
SMARTSM00809. Alpha_adaptinC2. 1 hit.
SM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMSSF55711. AP2_adap_app. 1 hit.
SSF48371. ARM-type_fold. 1 hit.
SSF49348. Clath_adapt. 1 hit.
ProtoNetSearch...

Other

NextBio609967.

Entry information

Entry nameAP1B1_RAT
AccessionPrimary (citable) accession number: P52303
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 25, 2012
This is version 93 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents