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Protein

AP-1 complex subunit beta-1

Gene

Ap1b1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Subunit of clathrin-associated adaptor protein complex 1 that plays a role in protein sorting in the late-Golgi/trans-Golgi network (TGN) and/or endosomes. The AP complexes mediate both the recruitment of clathrin to membranes and the recognition of sorting signals within the cytosolic tails of transmembrane cargo molecules.

GO - Molecular functioni

  • clathrin binding Source: BHF-UCL
  • protein transporter activity Source: InterPro

GO - Biological processi

  • clathrin coat assembly Source: BHF-UCL
  • intracellular protein transport Source: InterPro
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
AP-1 complex subunit beta-1
Alternative name(s):
Adaptor protein complex AP-1 subunit beta-1
Adaptor-related protein complex 1 subunit beta-1
Beta-1-adaptin
Beta-adaptin 1
Clathrin assembly protein complex 1 beta large chain
Golgi adaptor HA1/AP1 adaptin beta subunit
Gene namesi
Name:Ap1b1
Synonyms:Adtb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2064. Ap1b1.

Subcellular locationi

GO - Cellular componenti

  • clathrin adaptor complex Source: InterPro
  • clathrin coat Source: BHF-UCL
  • clathrin-coated vesicle membrane Source: UniProtKB-SubCell
  • Golgi apparatus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasmic vesicle, Golgi apparatus, Membrane

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 949949AP-1 complex subunit beta-1PRO_0000193740Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei318 – 3181N6-acetyllysineBy similarity
Modified residuei574 – 5741Nitrated tyrosineBy similarity

Post-translational modificationi

The N-terminus is blocked.

Keywords - PTMi

Acetylation, Nitration

Proteomic databases

PaxDbiP52303.
PRIDEiP52303.

PTM databases

iPTMnetiP52303.
PhosphoSiteiP52303.

Interactioni

Subunit structurei

Adaptor protein complex 1 (AP-1) is a heterotetramer composed of two large adaptins (gamma-type subunit AP1G1 and beta-type subunit AP1B1), a medium adaptin (mu-type subunit AP1M1 or AP1M2) and a small adaptin (sigma-type subunit AP1S1 or AP1S2 or AP1S3).1 Publication

GO - Molecular functioni

  • clathrin binding Source: BHF-UCL

Protein-protein interaction databases

IntActiP52303. 3 interactions.
MINTiMINT-1344522.
STRINGi10116.ENSRNOP00000054218.

Structurei

Secondary structure

1
949
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Beta strandi10 – 134
Helixi14 – 2310
Beta strandi24 – 263
Helixi27 – 4216
Turni48 – 503
Helixi51 – 555
Helixi63 – 7917
Helixi81 – 855
Helixi88 – 958
Helixi100 – 11112
Helixi118 – 13114
Helixi135 – 15016
Helixi158 – 1603
Helixi161 – 17111
Helixi176 – 18813
Helixi203 – 2119
Helixi216 – 22611
Helixi234 – 24916
Helixi254 – 26613
Helixi277 – 29216
Helixi296 – 31217
Helixi314 – 3163
Turni317 – 3193
Beta strandi328 – 3303
Helixi332 – 34413
Helixi348 – 36316
Helixi367 – 38317
Helixi385 – 3873
Helixi388 – 39912
Helixi404 – 42017
Helixi428 – 4336
Helixi442 – 45413
Turni455 – 4584
Helixi462 – 47110
Beta strandi474 – 4763
Helixi478 – 49215
Helixi500 – 51213
Helixi517 – 53216
Helixi534 – 5418
Helixi557 – 5648
Turni565 – 5684
Helixi571 – 5744
Helixi578 – 5814

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W63X-ray4.00B/D/F/H/J/L1-584[»]
ProteinModelPortaliP52303.
SMRiP52303. Positions 2-583, 717-949.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52303.

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi576 – 728153Pro-rich (stalk region)Add
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG1061. Eukaryota.
COG5096. LUCA.
HOGENOMiHOG000163270.
HOVERGENiHBG050515.
InParanoidiP52303.
PhylomeDBiP52303.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1150. 1 hit.
3.30.310.10. 1 hit.
InterProiIPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR012295. Beta2_adaptin/TBP_C_dom.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013037. Clathrin_b-adaptin_app_Ig-like.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 2 hits.
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002291. AP_complex_beta. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform A (identifier: P52303-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MTDSKYFTTT KKGEIFELKA ELNSDKKEKK KEAVKKVIAS MTVGKDVSAL
60 70 80 90 100
FPDVVNCMQT DNLELKKLVY LYLMNYAKSQ PDMAIMAVNT FVKDCEDPNP
110 120 130 140 150
LIRALAVRTM GCIRVDKITE YLCEPLRKCL KDEDPYVRKT AAVCVAKLHD
160 170 180 190 200
INAQLVEDQG FLDTLKDLIS DSNPMVVANR VAALSEIAES HPSSNLLDLK
210 220 230 240 250
AQSINKLLTA LNECTEWAQI FILDCLGNYM PKDDREAQSI CERVTPRLSH
260 270 280 290 300
ANSAVVLSAV KVLMKFMEML SKDLDYYATL LKKLAPPLVT LLSAEPEPQY
310 320 330 340 350
VPLRNINLIV QKRPEILKHE MKVFFVKYND PIYVKLEKLD IMIRLASQAN
360 370 380 390 400
IAQVLAELKE YATEVDVDFV RKAVRAIGRC AIKVEQSAER CVSTLLDLIQ
410 420 430 440 450
TKVNYVVQEA IVVIKDIFRK YPNKYESVIA TLCENLDSLD EPEARAAMIW
460 470 480 490 500
IVGEYAERID NADELLESFL DGFHDESTQV QLQLLTAIVK LFLKKPTETQ
510 520 530 540 550
ELVQQVLSLA TQDSDNPDLR DRGYIYWRLL STDPVAAKEV VLAEKPLISE
560 570 580 590 600
ETDLIEPTLL DELICYIGTL ASVYHKPPNA FVEGGRGVVH KSLPPRTASS
610 620 630 640 650
ESTESPEAAP AGAPASDQPD VIPAQGDLLG DLLNLDLGPP VSGPPLAASS
660 670 680 690 700
VQMGAVDLLG GGLDSLMGDE PEGIGDSNFG APPASVAAAA PARLGAPVSS
710 720 730 740 750
GLSDLFDLTS GVGTLSGSYV APKAVWLPAM KAKGLEISGT FTRQVGSISM
760 770 780 790 800
DLQLTNKALQ VMTDFAIQFN RNSFGLAPAA PLQVHAPLSP NQTVEISLPL
810 820 830 840 850
NTVGSVMKME PLNNLQVAVK NNIDVFYFST LYPLHVLFVE DGKMDRQMFL
860 870 880 890 900
ATWKDIPNEN EAQFQIRDCP LNTEAASSKL QSSNIFTVAK RTVEGQDMLY
910 920 930 940
QSLKLTNGIW VLAELRIQPG NPSFTLSLKC RAPEVSQHDI QAYETILKN
Length:949
Mass (Da):104,589
Last modified:October 1, 1996 - v1
Checksum:iCC5AE54E8AACCD44
GO
Isoform B (identifier: P52303-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     667-673: Missing.

Show »
Length:942
Mass (Da):103,873
Checksum:i69F7E7D2234C7BC1
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei667 – 6737Missing in isoform B. CuratedVSP_000164

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77245 mRNA. Translation: AAA40807.1.
PIRiB32105.
RefSeqiNP_058973.1. NM_017277.1. [P52303-1]
UniGeneiRn.37383.

Genome annotation databases

GeneIDi29663.
UCSCiRGD:2064. rat. [P52303-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M77245 mRNA. Translation: AAA40807.1.
PIRiB32105.
RefSeqiNP_058973.1. NM_017277.1. [P52303-1]
UniGeneiRn.37383.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1W63X-ray4.00B/D/F/H/J/L1-584[»]
ProteinModelPortaliP52303.
SMRiP52303. Positions 2-583, 717-949.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52303. 3 interactions.
MINTiMINT-1344522.
STRINGi10116.ENSRNOP00000054218.

PTM databases

iPTMnetiP52303.
PhosphoSiteiP52303.

Proteomic databases

PaxDbiP52303.
PRIDEiP52303.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi29663.
UCSCiRGD:2064. rat. [P52303-1]

Organism-specific databases

CTDi162.
RGDi2064. Ap1b1.

Phylogenomic databases

eggNOGiKOG1061. Eukaryota.
COG5096. LUCA.
HOGENOMiHOG000163270.
HOVERGENiHBG050515.
InParanoidiP52303.
PhylomeDBiP52303.

Miscellaneous databases

EvolutionaryTraceiP52303.
NextBioi609967.
PROiP52303.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
2.60.40.1150. 1 hit.
3.30.310.10. 1 hit.
InterProiIPR026739. AP_beta.
IPR016342. AP_complex_bsu_1_2_4.
IPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR015151. B-adaptin_app_sub_C.
IPR012295. Beta2_adaptin/TBP_C_dom.
IPR002553. Clathrin/coatomer_adapt-like_N.
IPR008152. Clathrin_a/b/g-adaptin_app_Ig.
IPR013037. Clathrin_b-adaptin_app_Ig-like.
IPR009028. Coatomer/calthrin_app_sub_C.
IPR013041. Coatomer/clathrin_app_Ig-like.
[Graphical view]
PANTHERiPTHR11134. PTHR11134. 2 hits.
PfamiPF01602. Adaptin_N. 1 hit.
PF02883. Alpha_adaptinC2. 1 hit.
PF09066. B2-adapt-app_C. 1 hit.
[Graphical view]
PIRSFiPIRSF002291. AP_complex_beta. 1 hit.
SMARTiSM00809. Alpha_adaptinC2. 1 hit.
SM01020. B2-adapt-app_C. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
SSF49348. SSF49348. 1 hit.
SSF55711. SSF55711. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Structural and functional division into two domains of the large (100- to 115-kDa) chains of the clathrin-associated protein complex AP-2."
    Kirchhausen T., Nathanson K.L., Matsui W., Vaisberg A., Chow E.P., Burne C., Keen J.H., Davis A.E.
    Proc. Natl. Acad. Sci. U.S.A. 86:2612-2616(1989) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], PARTIAL PROTEIN SEQUENCE.
    Tissue: Brain.
  2. "Characterization of a new member of the human beta-adaptin gene family from chromosome 22q12, a candidate meningioma gene."
    Peyrard M., Fransson I., Xie Y.-G., Han F.-Y., Ruttledge M.H., Swahn S., Collins J.E., Dunham I., Collins V.P., Dumanski J.P.
    Hum. Mol. Genet. 3:1393-1399(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: CHARACTERIZATION OF ISOFORMS A AND B.
  3. Cited for: X-RAY CRYSTALLOGRAPHY (4.0 ANGSTROMS) OF 1-584, SUBUNIT.

Entry informationi

Entry nameiAP1B1_RAT
AccessioniPrimary (citable) accession number: P52303
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 125 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Was originally thought to be part of the complex AP-2.1 Publication

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.