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Protein

Importin subunit beta-1

Gene

Kpnb1

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Functions in nuclear protein import, either in association with an adapter protein, like an importin-alpha subunit, which binds to nuclear localization signals (NLS) in cargo substrates, or by acting as autonomous nuclear transport receptor. Acting autonomously, serves itself as NLS receptor. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. Mediates autonomously the nuclear import of ribosomal proteins RPL23A, RPS7 and RPL5. Binds to a beta-like import receptor binding (BIB) domain of RPL23A. In association with IPO7 mediates the nuclear import of H1 histone. In vitro, mediates nuclear import of H2A, H2B, H3 and H4 histones. In case of HIV-1 infection, binds and mediates the nuclear import of HIV-1 Rev. Imports SNAI1 and PRKCI into the nucleus (By similarity).By similarity

GO - Molecular functioni

  • kinesin binding Source: RGD
  • nuclear localization sequence binding Source: GO_Central
  • protein complex binding Source: RGD
  • protein transporter activity Source: GO_Central

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit beta-1
Alternative name(s):
Karyopherin subunit beta-1
Nuclear factor p97
Pore targeting complex 97 kDa subunit
Short name:
PTAC97
Gene namesi
Name:Kpnb1
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi2909. Kpnb1.

Subcellular locationi

GO - Cellular componenti

  • cytoplasm Source: GO_Central
  • nuclear membrane Source: GO_Central
  • nuclear periphery Source: GO_Central
  • nuclear pore Source: RGD
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 875875Importin subunit beta-1PRO_0000120747Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei1 – 11N-acetylmethionineBy similarity
Modified residuei12 – 121PhosphoserineBy similarity
Cross-linki205 – 205Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin)By similarity
Modified residuei210 – 2101N6-acetyllysine; alternateBy similarity
Cross-linki210 – 210Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in ubiquitin); alternateBy similarity
Modified residuei834 – 8341N6-acetyllysineBy similarity
Modified residuei866 – 8661N6-acetyllysineBy similarity

Post-translational modificationi

Mono-ADP-ribosylated by PARP16.By similarity

Keywords - PTMi

Acetylation, ADP-ribosylation, Isopeptide bond, Phosphoprotein, Ubl conjugation

Proteomic databases

PaxDbiP52296.
PeptideAtlasiP52296.
PRIDEiP52296.

PTM databases

iPTMnetiP52296.
PhosphoSiteiP52296.

Interactioni

Subunit structurei

Forms a complex with an importin alpha subunit. Forms a heterodimer with IPO7. Interacts with IPO7, SNUPN, RPL23A and XPO1. The KPNB1/IPO7 heterodimer interacts with H1 histone. Interacts with H2A, H2B, H3 and H4 histones (By similarity). Component of an import snRNP complex composed of KPNB1, SNUPN, SMN1 and ZNF259. Component of a nuclear export receptor complex composed of KPNB1, Ran, SNUPN and XPO1. Binds to HIV-1 Rev and Tat. Interacts with HTLV-1 Rex. Interacts with SRY. Interacts with PRKCI/atypical protein kinase C iota (By similarity). Interacts with KPNA7. Interacts with SNAI1 (via zinc fingers) and SNAI2 (via zinc fingers) (By similarity). Interacts with SLC35G1 and STIM1 (By similarity). Interacts with DCAF8. Interacts with RAN.By similarity

GO - Molecular functioni

  • kinesin binding Source: RGD
  • protein complex binding Source: RGD

Protein-protein interaction databases

BioGridi247024. 2 interactions.
DIPiDIP-37024N.
IntActiP52296. 3 interactions.
MINTiMINT-4592883.
STRINGi10116.ENSRNOP00000012376.

Structurei

3D structure databases

ProteinModelPortaliP52296.
SMRiP52296. Positions 1-875.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati2 – 3130HEAT 1By similarityAdd
BLAST
Domaini21 – 10080Importin N-terminalPROSITE-ProRule annotationAdd
BLAST
Repeati33 – 6432HEAT 2By similarityAdd
BLAST
Repeati84 – 12239HEAT 3By similarityAdd
BLAST
Repeati128 – 15932HEAT 4By similarityAdd
BLAST
Repeati169 – 20133HEAT 5By similarityAdd
BLAST
Repeati211 – 24636HEAT 6By similarityAdd
BLAST
Repeati252 – 30150HEAT 7By similarityAdd
BLAST
Repeati313 – 35947HEAT 8By similarityAdd
BLAST
Repeati363 – 39331HEAT 9By similarityAdd
BLAST
Repeati401 – 43737HEAT 10By similarityAdd
BLAST
Repeati448 – 48437HEAT 11By similarityAdd
BLAST
Repeati499 – 53638HEAT 12By similarityAdd
BLAST
Repeati543 – 59149HEAT 13By similarityAdd
BLAST
Repeati599 – 63840HEAT 14By similarityAdd
BLAST
Repeati643 – 68038HEAT 15By similarityAdd
BLAST
Repeati685 – 72339HEAT 16By similarityAdd
BLAST
Repeati731 – 77545HEAT 17By similarityAdd
BLAST
Repeati785 – 82844HEAT 18By similarityAdd
BLAST
Repeati830 – 87243HEAT 19By similarityAdd
BLAST

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni328 – 34114IAB-bindingAdd
BLAST
Regioni333 – 41886Ran-GTP bindingBy similarityAdd
BLAST

Sequence similaritiesi

Contains 19 HEAT repeats.By similarity
Contains 1 importin N-terminal domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG1241. Eukaryota.
COG5215. LUCA.
HOGENOMiHOG000204108.
HOVERGENiHBG002369.
InParanoidiP52296.
KOiK14293.
PhylomeDBiP52296.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52296-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MELITILEKT VSPDRLELEA AQKFLERAAV ENLPTFLVEL SRVLANPGNS
60 70 80 90 100
QVARVAAGLQ IRLLTSKDPD IKAQYQQRWL AIDANARREV KNYVLQTLGT
110 120 130 140 150
ETYRPSSASQ CVAGIACAEI PVSQWPELIP QLVANVTNPN STEHMKESTL
160 170 180 190 200
EAIGYICQDI DPEQLQDKSN EILTAIIQGM RKEEPSNNVK LAATNALLNS
210 220 230 240 250
LEFTKANFDK ESERHFIMQV VCEATQCPDT RVRVAALQNL VKIMSLYYQY
260 270 280 290 300
METYMGPALF AITIEAMKSD IDEVALQGIE FWSNVCDEEM DLAIEASEAA
310 320 330 340 350
EQGRPPEHTS KFYAKGALQY LVPILTQTLT KQDENDDDDD WNPCKAAGVC
360 370 380 390 400
LMLLSTCCED DIVPHVLPFI KEHIKNPDWR YRDAAVMAFG SILEGPEPNQ
410 420 430 440 450
LKPLVIQAMP TLIELMKDPS VVVRDTTAWT VGRICELLPE AAINDVYLAP
460 470 480 490 500
LLQCLIEGLS AEPRVASNVC WAFSSLAEAA YEAADVADDQ EEPATYCLSS
510 520 530 540 550
SFELIVQKLL ETTDRPDGHQ NNLRSSAYES LMEIVKNSAK DCYPAVQKTT
560 570 580 590 600
LVIMERLQQV LQMESHIQST SDRIQFNDLQ SLLCATLQNV LWKVQHQDAL
610 620 630 640 650
QISDVVMASL LRMFQSTAGS GGVQEDALMA VSTLVEVLGG EFLKYMEAFK
660 670 680 690 700
PFLGIGLKNY AECQVCLAAV GLVGDLCRAL QSNILPFCDE VMQLLLENLG
710 720 730 740 750
NENVHRSVKP QILSVFGDIT LAIGGEFKKY LEVVLNTLQQ ASQAQVDKSD
760 770 780 790 800
FDMVDYLNEL RESCLEAYTG IVQGLKGDQE NVHPDVMLVQ PRVEFILSFI
810 820 830 840 850
DHIAGDEDHT DGVVACAAGL IGDLCTAFGK DVLKLVEARP MIHELLTEGR
860 870
RSKTNKAKTL ATWATKELRK LKNQA
Length:875
Mass (Da):97,124
Last modified:October 1, 1996 - v1
Checksum:i9CD77A05744014C4
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38644 mRNA. Translation: AAC42047.1.
PIRiI59350.
RefSeqiNP_058759.1. NM_017063.1.
UniGeneiRn.11061.

Genome annotation databases

GeneIDi24917.
KEGGirno:24917.
UCSCiRGD:2909. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L38644 mRNA. Translation: AAC42047.1.
PIRiI59350.
RefSeqiNP_058759.1. NM_017063.1.
UniGeneiRn.11061.

3D structure databases

ProteinModelPortaliP52296.
SMRiP52296. Positions 1-875.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi247024. 2 interactions.
DIPiDIP-37024N.
IntActiP52296. 3 interactions.
MINTiMINT-4592883.
STRINGi10116.ENSRNOP00000012376.

PTM databases

iPTMnetiP52296.
PhosphoSiteiP52296.

Proteomic databases

PaxDbiP52296.
PeptideAtlasiP52296.
PRIDEiP52296.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi24917.
KEGGirno:24917.
UCSCiRGD:2909. rat.

Organism-specific databases

CTDi3837.
RGDi2909. Kpnb1.

Phylogenomic databases

eggNOGiKOG1241. Eukaryota.
COG5215. LUCA.
HOGENOMiHOG000204108.
HOVERGENiHBG002369.
InParanoidiP52296.
KOiK14293.
PhylomeDBiP52296.

Miscellaneous databases

PROiP52296.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR000225. Armadillo.
IPR021133. HEAT_type_2.
IPR001494. Importin-beta_N.
[Graphical view]
PfamiPF03810. IBN_N. 1 hit.
[Graphical view]
SMARTiSM00185. ARM. 3 hits.
SM00913. IBN_N. 1 hit.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50077. HEAT_REPEAT. 1 hit.
PS50166. IMPORTIN_B_NT. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMB1_RAT
AccessioniPrimary (citable) accession number: P52296
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: September 7, 2016
This is version 129 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.