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Protein

Importin subunit alpha

Gene

Pen

Organism
Drosophila melanogaster (Fruit fly)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Promotes docking of import substrates to the nuclear envelope. Seems to act as a cytosolic receptor for both simple and bipartite NLS motifs (By similarity).By similarity
It is required for normal cell proliferation and may serve as an adapter molecule to form complexes with other proteins. May act as a tumor suppressor in hematopoietic cells. May play a role in the nuclear import of karyophilic proteins and some of these may be required for the normal transmission and function of proliferative signals in the cells.

GO - Molecular functioni

GO - Biological processi

  • cell proliferation Source: FlyBase
  • centrosome duplication Source: FlyBase
  • female germline ring canal formation Source: FlyBase
  • gravitaxis Source: FlyBase
  • lymph gland development Source: FlyBase
  • NLS-bearing protein import into nucleus Source: FlyBase
  • protein import into nucleus Source: FlyBase
  • protein import into nucleus, docking Source: FlyBase
  • sensory perception of pain Source: FlyBase
  • sperm individualization Source: FlyBase
Complete GO annotation...

Keywords - Biological processi

Protein transport, Transport

Enzyme and pathway databases

ReactomeiR-DME-5693548. Sensing of DNA Double Strand Breaks.
SignaLinkiP52295.

Names & Taxonomyi

Protein namesi
Recommended name:
Importin subunit alpha
Alternative name(s):
Karyopherin subunit alpha
Pendulin
Gene namesi
Name:Pen
ORF Names:CG4799
OrganismiDrosophila melanogaster (Fruit fly)
Taxonomic identifieri7227 [NCBI]
Taxonomic lineageiEukaryotaMetazoaEcdysozoaArthropodaHexapodaInsectaPterygotaNeopteraEndopterygotaDipteraBrachyceraMuscomorphaEphydroideaDrosophilidaeDrosophilaSophophora
Proteomesi
  • UP000000803 Componenti: Chromosome 2L

Organism-specific databases

FlyBaseiFBgn0267727. Pen.

Subcellular locationi

  • Cytoplasm
  • Nucleus

  • Note: Shuttles between the cytoplasm and nucleus in a cell cycle-dependent manner.

GO - Cellular componenti

  • cytoplasm Source: FlyBase
  • cytosol Source: GO_Central
  • lipid particle Source: FlyBase
  • microtubule associated complex Source: FlyBase
  • nuclear pore Source: GO_Central
  • nucleoplasm Source: GO_Central
  • nucleus Source: FlyBase
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 522522Importin subunit alphaPRO_0000120733Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei12 – 121Phosphoserine1 Publication
Modified residuei17 – 171Phosphoserine1 Publication
Modified residuei56 – 561Phosphoserine1 Publication
Modified residuei65 – 651Phosphoserine1 Publication

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiP52295.

PTM databases

iPTMnetiP52295.

Expressioni

Tissue specificityi

Predominantly expressed in the neuroblast stem cells.

Developmental stagei

High levels are detected during the first half of embryogenesis reaching a maximum between 4 and 8 hours of development. Protein expression increases again from the third larval instar onwards. It is expressed in a maternal/early embryonic phase, and again during morphogenesis in late larval and pupal stages.

Gene expression databases

BgeeiFBgn0011823.
GenevisibleiP52295. DM.

Interactioni

Subunit structurei

Forms a complex with importin beta subunit.

Protein-protein interaction databases

BioGridi60430. 40 interactions.
DIPiDIP-17105N.
IntActiP52295. 5 interactions.
MINTiMINT-292464.
STRINGi7227.FBpp0079527.

Structurei

3D structure databases

ProteinModelPortaliP52295.
SMRiP52295. Positions 32-492.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini1 – 5656IBBPROSITE-ProRule annotationAdd
BLAST
Repeati64 – 10542ARM 1Add
BLAST
Repeati106 – 14843ARM 2Add
BLAST
Repeati149 – 19042ARM 3Add
BLAST
Repeati191 – 23343ARM 4Add
BLAST
Repeati234 – 27542ARM 5Add
BLAST
Repeati276 – 31742ARM 6Add
BLAST
Repeati318 – 35942ARM 7Add
BLAST
Repeati360 – 40041ARM 8Add
BLAST
Repeati401 – 44747ARM 9Add
BLAST
Repeati448 – 48942ARM 10Add
BLAST

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi490 – 52233Asp/Glu-rich (acidic)Add
BLAST

Sequence similaritiesi

Belongs to the importin alpha family.Curated
Contains 10 ARM repeats.PROSITE-ProRule annotation
Contains 1 IBB domain.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
GeneTreeiENSGT00760000119094.
InParanoidiP52295.
KOiK15043.
OMAiIVPICIR.
OrthoDBiEOG091G095Z.
PhylomeDBiP52295.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 7 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 3 hits.
PS51214. IBB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52295-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSKADSNSRQ GSYKANSINT QDSRMRRHEV TIELRKSKKE DQMFKRRNIN
60 70 80 90 100
DEDLTSPLKE LNGQSPVQLS VDEIVAAMNS EDQERQFLGM QSARKMLSRE
110 120 130 140 150
RNPPIDLMIG HGIVPICIRF LQNTNNSMLQ FEAAWALTNI ASGTSDQTRC
160 170 180 190 200
VIEHNAVPHF VALLQSKSMN LAEQAVWALG NIAGDGAAAR DIVIHHNVID
210 220 230 240 250
GILPLINNET PLSFLRNIVW LMSNLCRNKN PSPPFDQVKR LLPVLSQLLL
260 270 280 290 300
SQDIQVLADA CWALSYVTDD DNTKIQAVVD SDAVPRLVKL LQMDEPSIIV
310 320 330 340 350
PALRSVGNIV TGTDQQTDVV IASGGLPRLG LLLQHNKSNI VKEAAWTVSN
360 370 380 390 400
ITAGNQKQIQ AVIQAGIFQQ LRTVLEKGDF KAQKEAAWAV TNTTTSGTPE
410 420 430 440 450
QIVDLIEKYK ILKPFIDLLD TKDPRTIKVV QTGLSNLFAL AEKLGGTENL
460 470 480 490 500
CLMVEEMGGL DKLETLQQHE NEEVYKKAYA IIDTYFSNGD DEAEQELAPQ
510 520
EVNGALEFNA TQPKAPEGGY TF
Length:522
Mass (Da):57,822
Last modified:December 1, 2000 - v2
Checksum:iB02773EB76812AD1
GO

Sequence cautioni

The sequence AAL29091 differs from that shown. Reason: Erroneous translation. Wrong choice of frame.Curated

Experimental Info

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sequence conflicti69 – 691L → P in AAA85260 (PubMed:7790350).Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12269 mRNA. Translation: AAA85260.1.
X85752 Genomic DNA. Translation: CAA59753.1.
AE014134 Genomic DNA. Translation: AAF52853.1.
AY061543 mRNA. Translation: AAL29091.1. Sequence problems.
BT003258 mRNA. Translation: AAO25015.1.
PIRiA57319.
RefSeqiNP_477041.1. NM_057693.5.
UniGeneiDm.3560.

Genome annotation databases

EnsemblMetazoaiFBtr0079937; FBpp0079527; FBgn0267727.
GeneIDi34338.
KEGGidme:Dmel_CG4799.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U12269 mRNA. Translation: AAA85260.1.
X85752 Genomic DNA. Translation: CAA59753.1.
AE014134 Genomic DNA. Translation: AAF52853.1.
AY061543 mRNA. Translation: AAL29091.1. Sequence problems.
BT003258 mRNA. Translation: AAO25015.1.
PIRiA57319.
RefSeqiNP_477041.1. NM_057693.5.
UniGeneiDm.3560.

3D structure databases

ProteinModelPortaliP52295.
SMRiP52295. Positions 32-492.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi60430. 40 interactions.
DIPiDIP-17105N.
IntActiP52295. 5 interactions.
MINTiMINT-292464.
STRINGi7227.FBpp0079527.

PTM databases

iPTMnetiP52295.

Proteomic databases

PaxDbiP52295.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblMetazoaiFBtr0079937; FBpp0079527; FBgn0267727.
GeneIDi34338.
KEGGidme:Dmel_CG4799.

Organism-specific databases

CTDi34338.
FlyBaseiFBgn0267727. Pen.

Phylogenomic databases

eggNOGiKOG0166. Eukaryota.
COG5064. LUCA.
GeneTreeiENSGT00760000119094.
InParanoidiP52295.
KOiK15043.
OMAiIVPICIR.
OrthoDBiEOG091G095Z.
PhylomeDBiP52295.

Enzyme and pathway databases

ReactomeiR-DME-5693548. Sensing of DNA Double Strand Breaks.
SignaLinkiP52295.

Miscellaneous databases

ChiTaRSiPen. fly.
GenomeRNAii34338.
PROiP52295.

Gene expression databases

BgeeiFBgn0011823.
GenevisibleiP52295. DM.

Family and domain databases

Gene3Di1.25.10.10. 1 hit.
InterProiIPR011989. ARM-like.
IPR016024. ARM-type_fold.
IPR032413. Arm_3.
IPR000225. Armadillo.
IPR002652. Importin-a_IBB.
IPR024931. Importing_su_alpha.
[Graphical view]
PfamiPF00514. Arm. 7 hits.
PF16186. Arm_3. 1 hit.
PF01749. IBB. 1 hit.
[Graphical view]
PIRSFiPIRSF005673. Importin_alpha. 1 hit.
SMARTiSM00185. ARM. 8 hits.
[Graphical view]
SUPFAMiSSF48371. SSF48371. 1 hit.
PROSITEiPS50176. ARM_REPEAT. 3 hits.
PS51214. IBB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiIMA_DROME
AccessioniPrimary (citable) accession number: P52295
Secondary accession number(s): Q24431, Q95R96, Q9VL45
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: December 1, 2000
Last modified: September 7, 2016
This is version 148 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programDrosophila annotation project

Miscellaneousi

Caution

Was originally thought to be the overgrown hematopoietic organs-31 protein (OHO-31).1 Publication

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Drosophila
    Drosophila: entries, gene names and cross-references to FlyBase
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.