Reviewed, UniProtKB/Swiss-Prot P52289 (PPA5_KLULA)

Names and origin

Protein names

Recommended name:
    Repressible acid phosphatase
    EC=3.1.3.2

Gene names

Name:	PHO5
Ordered Locus Names:	KLLA0B00286g

Organism

Kluyveromyces lactis (Yeast) (Candida sphaerica) [Complete proteome]

Taxonomic identifier

28985 [NCBI]

Taxonomic lineage

Eukaryota › Fungi › Dikarya › Ascomycota › Saccharomycotina › Saccharomycetes › Saccharomycetales › Saccharomycetaceae › Kluyveromyces

Protein attributes

Sequence length	469 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Catalytic activity	A phosphate monoester + H2O = an alcohol + phosphate.
Subcellular location	Secreted.
Induction	Repressed by inorganic phosphate By similarity.
Post-translational modification	Glycosylated during secretion across the membrane.
Sequence similarities	Belongs to the histidine acid phosphatase family.

Ontologies

Keywords
Cellular component	Secreted
Domain	Signal
Molecular function	Hydrolase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	extracellular region Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	acid phosphatase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 16	16	Potential
	Chain	17 – 469	453	Repressible acid phosphatase		PRO_0000023955
Sites
	Active site	77	1	Nucleophile By similarity
	Active site	340	1	Proton donor By similarity
Amino acid modifications
	Glycosylation	23	1	N-linked (GlcNAc...) Potential
	Glycosylation	31	1	N-linked (GlcNAc...) Potential
	Glycosylation	129	1	N-linked (GlcNAc...) Potential
	Glycosylation	201	1	N-linked (GlcNAc...) Potential
	Glycosylation	229	1	N-linked (GlcNAc...) Potential
	Glycosylation	250	1	N-linked (GlcNAc...) Potential
	Glycosylation	317	1	N-linked (GlcNAc...) Potential
	Glycosylation	392	1	N-linked (GlcNAc...) Potential
	Glycosylation	447	1	N-linked (GlcNAc...) Potential
Experimental info
	Sequence conflict	7	1	S → G in CAA83964. Ref.1
	Sequence conflict	32	1	S → N in CAA83964. Ref.1
	Sequence conflict	41	1	P → S in CAA83964. Ref.1
	Sequence conflict	106 – 108	3	GPM → DPL in CAA83964. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52289-1 [UniParc]. Last modified September 27, 2004. Version 2. Checksum: C6169701E485691A	FASTA	469	52,505
	10 20 30 40 50 60 MLSILLSLLS LSGTHAAPIS KDNGTVCYAL NSSTTDESIF PLLNGQGPHY DYPQSFGIPV 70 80 90 100 110 120 EVPDQCTVEH VQMLARHGER YPTASKGKLM IALWDKLKEF QGQYNGPMEV FNDYEFFVSN 130 140 150 160 170 180 TKYFDQLTNS TDVDPSNPYA GAKTAQHLGK YIAYNYGDLF SDSNPVFTSS SGRVHQTAKY 190 200 210 220 230 240 VVSSLEEELD IQLDLQIIQE NETSGANSLT PADSCMTYNG DLGDEYFENA TLPYLTDIKN 250 260 270 280 290 300 RWMKKNSNLN LTLEHDDIEL LVDWCAFETN VKGSSAVCDL FERNDLVAYS YYANVNNFYR 310 320 330 340 350 360 RGAGNPMSNP IGSVLVNASY NLLTQADELD NKVWLSFSHD TDIQQFISAL GLIDNGVTEY 370 380 390 400 410 420 SLDQVDFQNI QQLSWVTPMG GRIFTEKLKC GNASYVRYII NDVIIPVPGC TSGPGFSCPI 430 440 450 460 EDFDDYITNR LNGIDYVSSC EVQQVSNTTE LTFYWDYNEV EYNGPVSNK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	Ferminan E. Thesis (1995), University of Salamanca, Spain Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 76492 / CBS 2359/152 / CLIB 210.
[2]	"Genome evolution in yeasts." Dujon B., Sherman D., Fischer G., Durrens P., Casaregola S., Lafontaine I., de Montigny J., Marck C., Neuveglise C., Talla E., Goffard N., Frangeul L., Aigle M., Anthouard V., Babour A., Barbe V., Barnay S., Blanchin S. , Beckerich J.-M., Beyne E., Bleykasten C., Boisrame A., Boyer J., Cattolico L., Confanioleri F., de Daruvar A., Despons L., Fabre E., Fairhead C., Ferry-Dumazet H., Groppi A., Hantraye F., Hennequin C., Jauniaux N., Joyet P., Kachouri R., Kerrest A., Koszul R., Lemaire M., Lesur I., Ma L., Muller H., Nicaud J.-M., Nikolski M., Oztas S., Ozier-Kalogeropoulos O., Pellenz S., Potier S., Richard G.-F., Straub M.-L., Suleau A., Swennen D., Tekaia F., Wesolowski-Louvel M., Westhof E., Wirth B., Zeniou-Meyer M., Zivanovic Y., Bolotin-Fukuhara M., Thierry A., Bouchier C., Caudron B., Scarpelli C., Gaillardin C., Weissenbach J., Wincker P., Souciet J.-L. Nature 430:35-44(2004) [PubMed: 15229592] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 8585 / CBS 2359 / DSM 70799 / NRRL Y-1140 / WM37.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z33995 Genomic DNA. Translation: CAA83964.1. CR382122 Genomic DNA. Translation: CAH01935.1.
RefSeq	XP_451542.1.
3D structure databases
SMR	P52289. Positions 35-469.
ModBase	Search...
Genome annotation databases
GeneID	2896996.
GenomeReviews	Gene locus KLLA0B00286g in contig CR382122_GR.
KEGG	kla:KLLA0B00286g.
Phylogenomic databases
HOGENOM	HBG331692.
OMA	YMRHGER.
OrthoDB	EOG9RBS1T.
PhylomeDB	P52289.
Enzyme and pathway databases
BRENDA	3.1.3.2. 74088.
Family and domain databases
InterPro	IPR000560. Histidine_acid_Pase. IPR016274. Histidine_acid_Pase_euk. [Graphical view]
Pfam	PF00328. Acid_phosphat_A. 1 hit. [Graphical view]
PIRSF	PIRSF000894. Acid_phosphatase. 1 hit.
PROSITE	PS00616. HIS_ACID_PHOSPHAT_1. 1 hit. PS00778. HIS_ACID_PHOSPHAT_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PPA5_KLULA

Accession

Primary (citable) accession number: P52289
Secondary accession number(s): Q6CWZ7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	September 27, 2004
Last modified:	January 19, 2010
This is version 60 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

FPAP (Fungal Proteome Annotation Project)