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Protein

Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3

Gene

Gnb3

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Guanine nucleotide-binding proteins (G proteins) are involved as a modulator or transducer in various transmembrane signaling systems. The beta and gamma chains are required for the GTPase activity, for replacement of GDP by GTP, and for G protein-effector interaction.

GO - Molecular functioni

  • signal transducer activity Source: UniProtKB-KW
  • spectrin binding Source: MGI
Complete GO annotation...

Keywords - Molecular functioni

Transducer

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-381771. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Names & Taxonomyi

Protein namesi
Recommended name:
Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3
Alternative name(s):
Transducin beta chain 3
Gene namesi
Name:Gnb3
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Chromosome 4

Organism-specific databases

RGDi708344. Gnb3.

Subcellular locationi

GO - Cellular componenti

  • cell body Source: RGD
  • dendrite Source: Ensembl
  • extracellular exosome Source: Ensembl
  • neuron projection Source: RGD
Complete GO annotation...

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 340340Guanine nucleotide-binding protein G(I)/G(S)/G(T) subunit beta-3PRO_0000127701Add
BLAST

Proteomic databases

PaxDbiP52287.
PRIDEiP52287.

PTM databases

PhosphoSiteiP52287.

Expressioni

Tissue specificityi

Expressed at a high level in the heart and at a much lower level in the brain.

Gene expression databases

GenevisibleiP52287. RN.

Interactioni

Subunit structurei

G proteins are composed of 3 units, alpha, beta and gamma. Interacts with RASD2 (By similarity).By similarity

GO - Molecular functioni

  • spectrin binding Source: MGI

Protein-protein interaction databases

BioGridi248842. 1 interaction.
STRINGi10116.ENSRNOP00000021480.

Structurei

3D structure databases

ProteinModelPortaliP52287.
SMRiP52287. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Repeati53 – 8331WD 1Add
BLAST
Repeati95 – 12531WD 2Add
BLAST
Repeati141 – 17030WD 3Add
BLAST
Repeati182 – 21231WD 4Add
BLAST
Repeati224 – 25431WD 5Add
BLAST
Repeati268 – 29831WD 6Add
BLAST
Repeati310 – 34031WD 7Add
BLAST

Sequence similaritiesi

Belongs to the WD repeat G protein beta family.Curated
Contains 7 WD repeats.PROSITE-ProRule annotation

Keywords - Domaini

Repeat, WD repeat

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP52287.
KOiK07825.
OMAiDSMKCER.
OrthoDBiEOG7GN2N5.
PhylomeDBiP52287.
TreeFamiTF106149.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52287-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MGEMEQLKQE AEQLKKQIAD ARKACADITL AELVSGLEVV GRVQMRTRRT
60 70 80 90 100
LRGHLAKIYA MHWATDSKLL VSASQDGKLI VWDTYTTNKV HAIPLRSSWV
110 120 130 140 150
MTCAYAPSGN FVACGGLDNM CSIYSLKSRE GNVKVSRELS AHTGYLSCCR
160 170 180 190 200
FLDDNNIVTS SGDTTCALWD IETGQQKTVF VGHTGDCMSL AVSPDYKLFI
210 220 230 240 250
SGACDASAKL WDVREGTCRQ TFTGHESDIN AICFFPNGEA ICTGSDDASC
260 270 280 290 300
RLFDLRADQE LTAYSHESII CGITSVAFSL SGRLLFAGYD DFNCNVWDSL
310 320 330 340
KCERVGVLSG HDNRVSCLGV TADGMAVATG SWDSFLKIWN
Length:340
Mass (Da):37,180
Last modified:October 1, 1996 - v1
Checksum:i020A41F754E4B1DA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29090 Genomic DNA. Translation: AAA62620.1.
BC086422 mRNA. Translation: AAH86422.1.
PIRiI53871.
RefSeqiNP_068630.1. NM_021858.3.
UniGeneiRn.27065.

Genome annotation databases

EnsembliENSRNOT00000021481; ENSRNOP00000021480; ENSRNOG00000015541.
ENSRNOT00000090898; ENSRNOP00000069551; ENSRNOG00000015541.
GeneIDi60449.
KEGGirno:60449.
UCSCiRGD:708344. rat.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L29090 Genomic DNA. Translation: AAA62620.1.
BC086422 mRNA. Translation: AAH86422.1.
PIRiI53871.
RefSeqiNP_068630.1. NM_021858.3.
UniGeneiRn.27065.

3D structure databases

ProteinModelPortaliP52287.
SMRiP52287. Positions 1-340.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi248842. 1 interaction.
STRINGi10116.ENSRNOP00000021480.

PTM databases

PhosphoSiteiP52287.

Proteomic databases

PaxDbiP52287.
PRIDEiP52287.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSRNOT00000021481; ENSRNOP00000021480; ENSRNOG00000015541.
ENSRNOT00000090898; ENSRNOP00000069551; ENSRNOG00000015541.
GeneIDi60449.
KEGGirno:60449.
UCSCiRGD:708344. rat.

Organism-specific databases

CTDi2784.
RGDi708344. Gnb3.

Phylogenomic databases

eggNOGiKOG0286. Eukaryota.
ENOG410XQUX. LUCA.
GeneTreeiENSGT00760000119239.
HOGENOMiHOG000176356.
HOVERGENiHBG000188.
InParanoidiP52287.
KOiK07825.
OMAiDSMKCER.
OrthoDBiEOG7GN2N5.
PhylomeDBiP52287.
TreeFamiTF106149.

Enzyme and pathway databases

ReactomeiR-RNO-1296041. Activation of G protein gated Potassium channels.
R-RNO-163359. Glucagon signaling in metabolic regulation.
R-RNO-381676. Glucagon-like Peptide-1 (GLP1) regulates insulin secretion.
R-RNO-381771. Synthesis, secretion, and inactivation of Glucagon-like Peptide-1 (GLP-1).
R-RNO-400042. Adrenaline,noradrenaline inhibits insulin secretion.
R-RNO-420092. Glucagon-type ligand receptors.
R-RNO-432040. Vasopressin regulates renal water homeostasis via Aquaporins.
R-RNO-997272. Inhibition of voltage gated Ca2+ channels via Gbeta/gamma subunits.

Miscellaneous databases

NextBioi612200.
PROiP52287.

Gene expression databases

GenevisibleiP52287. RN.

Family and domain databases

Gene3Di2.130.10.10. 1 hit.
InterProiIPR020472. G-protein_beta_WD-40_rep.
IPR001632. Gprotein_B.
IPR016346. Guanine_nucleotide-bd_bsu.
IPR015943. WD40/YVTN_repeat-like_dom.
IPR001680. WD40_repeat.
IPR019775. WD40_repeat_CS.
IPR017986. WD40_repeat_dom.
[Graphical view]
PANTHERiPTHR19850. PTHR19850. 1 hit.
PfamiPF00400. WD40. 6 hits.
[Graphical view]
PRINTSiPR00319. GPROTEINB.
PR00320. GPROTEINBRPT.
SMARTiSM00320. WD40. 7 hits.
[Graphical view]
SUPFAMiSSF50978. SSF50978. 1 hit.
PROSITEiPS00678. WD_REPEATS_1. 3 hits.
PS50082. WD_REPEATS_2. 5 hits.
PS50294. WD_REPEATS_REGION. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Cloning and sequencing of a rat heart cDNA encoding a G-protein beta subunit related to the human retinal beta 3 subunit."
    Ray K., Robishaw J.D.
    Gene 149:337-340(1994) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA].
    Strain: Sprague-Dawley.
    Tissue: Heart.
  2. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Heart.
  3. Lubec G., Chen W.-Q.
    Submitted (APR-2007) to UniProtKB
    Cited for: PROTEIN SEQUENCE OF 69-78, IDENTIFICATION BY MASS SPECTROMETRY.
    Strain: Sprague-Dawley.
    Tissue: Hippocampus.

Entry informationi

Entry nameiGBB3_RAT
AccessioniPrimary (citable) accession number: P52287
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: May 11, 2016
This is version 115 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.