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Protein

Suppressor of kinetochore protein 1

Gene

SKP1

Organism
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Essential component of the E3 ubiquitin ligase complex SCF (SKP1-CUL1-F-box protein) ubiquitin ligase complex, which mediates the ubiquitination and subsequent proteasomal degradation of target proteins like phosphorylated SIC1. Participates in the attachment of chromosomes to the spindle. Acts as a regulatory component of the centromere DNA-binding protein complex CBF3, which is essential for chromosome segregation and movement of centromeres along microtubules. CBF3 is required for the recruitment of other kinetochore complexes to CEN DNA. It plays a role in the attachment of chromosomes to the spindle and binds selectively to a highly conserved DNA sequence called CDEIII, found in centromeres and in several promoters. The association of CBF3C with CBF3D and SGT1 is required for CBF3C activation and CBF3 assembly. SKP1/CBF3D could retrieve cyclins or cyclin-CDK-like proteins into the kinetochore thus providing cell cycle-regulated kinetochore activity. Involved in the regulation of methionine biosynthesis genes. Facilitates association of CDC53 with CDC4 and of ROY1 with YPT52.7 Publications

Pathwayi: protein ubiquitination

This protein is involved in the pathway protein ubiquitination, which is part of Protein modification.
View all proteins of this organism that are known to be involved in the pathway protein ubiquitination and in Protein modification.

GO - Molecular functioni

  • DNA replication origin binding Source: SGD

GO - Biological processi

  • cellular protein complex assembly Source: SGD
  • exit from mitosis Source: SGD
  • G1/S transition of mitotic cell cycle Source: SGD
  • G2/M transition of mitotic cell cycle Source: SGD
  • kinetochore assembly Source: SGD
  • negative regulation of cytoplasmic translation Source: SGD
  • protein neddylation Source: SGD
  • protein ubiquitination involved in ubiquitin-dependent protein catabolic process Source: SGD
  • regulation of exit from mitosis Source: SGD
  • regulation of protein complex assembly Source: SGD
  • SCF-dependent proteasomal ubiquitin-dependent protein catabolic process Source: SGD
  • septin ring assembly Source: SGD
  • vacuolar acidification Source: SGD

Keywordsi

Molecular functionDNA-binding
Biological processUbl conjugation pathway

Enzyme and pathway databases

BioCyciYEAST:G3O-29884-MONOMER.
ReactomeiR-SCE-174113. SCF-beta-TrCP mediated degradation of Emi1.
R-SCE-8854050. FBXL7 down-regulates AURKA during mitotic entry and in early mitosis.
R-SCE-8951664. Neddylation.
R-SCE-917937. Iron uptake and transport.
R-SCE-983168. Antigen processing: Ubiquitination & Proteasome degradation.
UniPathwayiUPA00143.

Names & Taxonomyi

Protein namesi
Recommended name:
Suppressor of kinetochore protein 1
Alternative name(s):
Centromere DNA-binding protein complex CBF3 subunit D
E3 ubiquitin ligase complex SCF subunit SKP1
Gene namesi
Name:SKP1
Synonyms:CBF3D
Ordered Locus Names:YDR328C
ORF Names:D9798.14
OrganismiSaccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Taxonomic identifieri559292 [NCBI]
Taxonomic lineageiEukaryotaFungiDikaryaAscomycotaSaccharomycotinaSaccharomycetesSaccharomycetalesSaccharomycetaceaeSaccharomyces
Proteomesi
  • UP000002311 Componenti: Chromosome IV

Organism-specific databases

EuPathDBiFungiDB:YDR328C.
SGDiS000002736. SKP1.

Subcellular locationi

GO - Cellular componenti

  • CBF3 complex Source: SGD
  • condensed chromosome kinetochore Source: UniProtKB-SubCell
  • cytoplasm Source: SGD
  • kinetochore Source: SGD
  • nucleus Source: SGD
  • RAVE complex Source: SGD
  • SCF ubiquitin ligase complex Source: SGD

Keywords - Cellular componenti

Centromere, Chromosome, Cytoplasm, Kinetochore, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemoved1 Publication
ChainiPRO_00001872572 – 194Suppressor of kinetochore protein 1Add BLAST193

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei177PhosphothreonineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

MaxQBiP52286.
PRIDEiP52286.

PTM databases

iPTMnetiP52286.

Interactioni

Subunit structurei

Component of the E3 ubiquitin ligase complexes SCF with HRT1, some cullins like CDC53, and some F-box proteins like MET30 CDC4 and SAF1. Interacts with CDC53 and MET30 to form the E3 ubiquitin ligase complex SCF(Met30) which also contains MET4. Forms complex SCF(Cdc4) together with CDC4 and CDC53. Component of the CBF3 complex, which is formed of CBF3A/CBF2, CBF3B/CEP3, CBF3C/CTF13 and CBF3D. Component of the RAVE complex composed of RAV1, RAV2 and SKP1/CBF3D. Interacts with RCY1, ROY1, CBF3D and SGT1.11 Publications

Binary interactionsi

Show more details

Protein-protein interaction databases

BioGridi32385. 148 interactors.
DIPiDIP-1236N.
IntActiP52286. 74 interactors.
MINTiMINT-384072.
STRINGi4932.YDR328C.

Structurei

Secondary structure

1194
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi5 – 9Combined sources5
Beta strandi15 – 19Combined sources5
Helixi20 – 23Combined sources4
Helixi27 – 34Combined sources8
Beta strandi76 – 78Combined sources3
Helixi84 – 96Combined sources13
Turni97 – 99Combined sources3
Helixi118 – 123Combined sources6
Helixi128 – 140Combined sources13
Helixi144 – 158Combined sources15
Helixi163 – 170Combined sources8
Helixi178 – 185Combined sources8

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1NEXX-ray2.70A/C1-194[»]
3MKSX-ray2.60A/C2-194[»]
3V7DX-ray2.31A/C1-194[»]
5AN3X-ray2.82D1-35[»]
D65-158[»]
ProteinModelPortaliP52286.
SMRiP52286.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52286.

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni135 – 194Interaction with the F-box domain of F-box proteinsBy similarityAdd BLAST60

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi66 – 74Asp/Glu-rich (highly acidic)9

Sequence similaritiesi

Belongs to the SKP1 family.Curated

Phylogenomic databases

GeneTreeiENSGT00390000012652.
HOGENOMiHOG000172184.
InParanoidiP52286.
KOiK03094.
OMAiENKWCEE.
OrthoDBiEOG092C54Q0.

Family and domain databases

InterProiView protein in InterPro
IPR016897. SKP1.
IPR001232. SKP1-like.
IPR011333. SKP1/BTB/POZ.
IPR016072. Skp1_comp_dimer.
IPR016073. Skp1_comp_POZ.
PfamiView protein in Pfam
PF01466. Skp1. 1 hit.
PF03931. Skp1_POZ. 2 hits.
PIRSFiPIRSF028729. E3_ubiquit_lig_SCF_Skp. 1 hit.
SMARTiView protein in SMART
SM00512. Skp1. 1 hit.
SUPFAMiSSF54695. SSF54695. 2 hits.
SSF81382. SSF81382. 1 hit.

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52286-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MVTSNVVLVS GEGERFTVDK KIAERSLLLK NYLNDMHDSN LQNNSDSESD
60 70 80 90 100
SDSETNHKSK DNNNGDDDDE DDDEIVMPVP NVRSSVLQKV IEWAEHHRDS
110 120 130 140 150
NFPDEDDDDS RKSAPVDSWD REFLKVDQEM LYEIILAANY LNIKPLLDAG
160 170 180 190
CKVVAEMIRG RSPEEIRRTF NIVNDFTPEE EAAIRRENEW AEDR
Length:194
Mass (Da):22,330
Last modified:January 23, 2007 - v2
Checksum:i746DDE6470A69432
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti48E → D in AAC49492 (PubMed:8706131).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U43179 Genomic DNA. Translation: AAB17500.1.
U61764 mRNA. Translation: AAC49492.1.
U32517 Genomic DNA. Translation: AAB64763.1.
AY557730 Genomic DNA. Translation: AAS56056.1.
BK006938 Genomic DNA. Translation: DAA12170.1.
PIRiS59793.
RefSeqiNP_010615.3. NM_001180636.3.

Genome annotation databases

EnsemblFungiiYDR328C; YDR328C; YDR328C.
GeneIDi851928.
KEGGisce:YDR328C.

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.

Entry informationi

Entry nameiSKP1_YEAST
AccessioniPrimary (citable) accession number: P52286
Secondary accession number(s): D6VSW0, Q07186
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: July 5, 2017
This is version 169 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programFungal Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  3. SIMILARITY comments
    Index of protein domains and families
  4. Yeast
    Yeast (Saccharomyces cerevisiae): entries, gene names and cross-references to SGD
  5. Yeast chromosome IV
    Yeast (Saccharomyces cerevisiae) chromosome IV: entries and gene names