P52284 (MTR1_PBCVX) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Modification methylase CviRI
Short name=M.CviRI
EC=2.1.1.72

Alternative name(s):
Adenine-specific methyltransferase CviRI

Gene names

Name:

CVIRIM

Organism

Paramecium bursaria Chlorella virus XZ-6E (PBCV-XZ-6E)

Taxonomic identifier

36360 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus › unclassified Chlorovirus

Virus host

Chlorella [TaxID: 114049]

Protein attributes

Sequence length	379 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	This methylase recognizes the double-stranded sequence TGCA, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the CviRI endonuclease.
Catalytic activity	S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.
Sequence similarities	Belongs to the N(4)/N(6)-methyltransferase family.

Ontologies

Keywords
Biological process	Restriction system
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
Gene Ontology (GO)
Biological_process	DNA methylation on adenine Inferred from electronic annotation. Source: GOC DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular_function	nucleic acid binding Inferred from electronic annotation. Source: InterPro site-specific DNA-methyltransferase (adenine-specific) activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 379	379	Modification methylase CviRI		PRO_0000087949

Sequences

	Sequence		Length	Mass (Da)
	P52284 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 22758935BB702BFA	FASTA	379	42,815
	10 20 30 40 50 60 MKLFEDKSVE FHKRLSKKER SDGGVFFTPK DIRDIVFEEL GDFEPTNILE PTCGTGEFIS 70 80 90 100 110 120 DCRKVYKNSR IIGVEIDPRS AELARDGSKN EIIVHDFMTW DTDEKFDLII GNPPYFTRPT 130 140 150 160 170 180 GFKHDPSVVK CRSNICIEVL HKCITRHLAD NGMLAMVLPV SILNSKFYTP TIDLITDTMD 190 200 210 220 230 240 VVSARAIKKN NFMGTNVRVM VFIIRKRTPG FVSKYTFKTS LGKVIINPDG ERLGSIVSGK 250 260 270 280 290 300 KTIGSLNVNI SFGVTLASVK EYFVDKSCSG SFPLICYNNI AKKGDLLFVS DKYSKKRFNG 310 320 330 340 350 360 RAILIPRGYA HGDYSFNFID YTNDYFIIEN HVIAITGEDC VLDIIAKSFA DHRTREFCRL 370 LCSSGDISKD YVKEIPVFG

References

[1]	"Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E." Stefan G., Xia Y., van Etten J.L. Nucleic Acids Res. 19:307-311(1991) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	M38173 Genomic DNA. Translation: AAA42900.1.
3D structure databases
ProteinModelPortal	P52284.
ModBase	Search...
Protein family/group databases
REBASE	3355. M.CviRI.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR002052. DNA_methylase_N6_adenine_CS. IPR002296. N12N6_MeTrfase. [Graphical view]
PRINTS	PR00507. N12N6MTFRASE.
PROSITE	PS00092. N6_MTASE. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MTR1_PBCVX

Accession

Primary (citable) accession number: P52284

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	April 3, 2013
This is version 54 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Viral Protein Annotation Program