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Protein

Modification methylase CviRI

Gene

CVIRIM

Organism
Paramecium bursaria Chlorella virus XZ-6E (PBCV-XZ-6E)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

This methylase recognizes the double-stranded sequence TGCA, causes specific methylation on A-4 on both strands, and protects the DNA from cleavage by the CviRI endonuclease.

Catalytic activityi

S-adenosyl-L-methionine + DNA adenine = S-adenosyl-L-homocysteine + DNA 6-methylaminopurine.

GO - Molecular functioni

  1. nucleic acid binding Source: InterPro
  2. site-specific DNA-methyltransferase (adenine-specific) activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

Restriction system

Keywords - Ligandi

S-adenosyl-L-methionine

Protein family/group databases

REBASEi3355. M.CviRI.

Names & Taxonomyi

Protein namesi
Recommended name:
Modification methylase CviRI (EC:2.1.1.72)
Short name:
M.CviRI
Alternative name(s):
Adenine-specific methyltransferase CviRI
Gene namesi
Name:CVIRIM
OrganismiParamecium bursaria Chlorella virus XZ-6E (PBCV-XZ-6E)
Taxonomic identifieri36360 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirusunclassified Chlorovirus
Virus hostiChlorella [TaxID: 114049]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 379379Modification methylase CviRIPRO_0000087949Add
BLAST

Structurei

3D structure databases

ProteinModelPortaliP52284.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the N(4)/N(6)-methyltransferase family.Curated

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52284-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKLFEDKSVE FHKRLSKKER SDGGVFFTPK DIRDIVFEEL GDFEPTNILE
60 70 80 90 100
PTCGTGEFIS DCRKVYKNSR IIGVEIDPRS AELARDGSKN EIIVHDFMTW
110 120 130 140 150
DTDEKFDLII GNPPYFTRPT GFKHDPSVVK CRSNICIEVL HKCITRHLAD
160 170 180 190 200
NGMLAMVLPV SILNSKFYTP TIDLITDTMD VVSARAIKKN NFMGTNVRVM
210 220 230 240 250
VFIIRKRTPG FVSKYTFKTS LGKVIINPDG ERLGSIVSGK KTIGSLNVNI
260 270 280 290 300
SFGVTLASVK EYFVDKSCSG SFPLICYNNI AKKGDLLFVS DKYSKKRFNG
310 320 330 340 350
RAILIPRGYA HGDYSFNFID YTNDYFIIEN HVIAITGEDC VLDIIAKSFA
360 370
DHRTREFCRL LCSSGDISKD YVKEIPVFG
Length:379
Mass (Da):42,815
Last modified:October 1, 1996 - v1
Checksum:i22758935BB702BFA
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38173 Genomic DNA. Translation: AAA42900.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
M38173 Genomic DNA. Translation: AAA42900.1.

3D structure databases

ProteinModelPortaliP52284.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi3355. M.CviRI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
InterProiIPR002052. DNA_methylase_N6_adenine_CS.
IPR002296. N12N6_MeTrfase.
IPR029063. SAM-dependent_MTases.
[Graphical view]
PRINTSiPR00507. N12N6MTFRASE.
SUPFAMiSSF53335. SSF53335. 1 hit.
PROSITEiPS00092. N6_MTASE. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Molecular cloning and characterization of the gene encoding the adenine methyltransferase M.CviRI from Chlorella virus XZ-6E."
    Stefan G., Xia Y., van Etten J.L.
    Nucleic Acids Res. 19:307-311(1991) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].

Entry informationi

Entry nameiMTR1_PBCVX
AccessioniPrimary (citable) accession number: P52284
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: January 7, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.