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Protein

Type-2 restriction enzyme CviJI

Gene

CVJIR

Organism
Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence RGCY and cleaves after G-2. In the presence of ATP, there is a relaxation of its specificity and it can cleave RGCN and YGCY, but not YGCR.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionEndonuclease, Hydrolase, Nuclease
Biological processRestriction system
LigandMagnesium

Protein family/group databases

REBASEi760. CviJI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme CviJI (EC:3.1.21.4)
Short name:
R.CviJI
Alternative name(s):
Endonuclease CviJI
Type II restriction enzyme CviJI
Gene namesi
Name:CVJIR
OrganismiParamecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Taxonomic identifieri46019 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 3071]

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00000772981 – 278Type-2 restriction enzyme CviJIAdd BLAST278

Structurei

3D structure databases

SMRiP52283.
ModBaseiSearch...
MobiDBiSearch...

Sequencei

Sequence statusi: Complete.

P52283-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEEKKRLALI EKQRIAEEKI ASGRKIRKRI STNATKHERE FVKVINSMFV
60 70 80 90 100
GPATFVFVDI KGNKSREIHN VVRFRQLQGS KAKSPTAYVD REYNKPKADI
110 120 130 140 150
AAVDITGKDV AWISHKASEG YQQYLKISGK NLKFTGKELE EVLSFKRKVV
160 170 180 190 200
SMAPVSKIWP ANKTVWSPIK SNLIKNQAIF GFDYGKKPGR DNVDIIGQGR
210 220 230 240 250
PIITKRGSIL YLTFTGFSAL NGHLENFTGK HEPVFYVRTE RSSSGRSITT
260 270
VVNGVTYKNL RFFIHPYNFV SSKTQRIM
Length:278
Mass (Da):31,634
Last modified:October 1, 1996 - v1
Checksum:iB348AD778C6A1EA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09001 Genomic DNA. Translation: AAC55064.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09001 Genomic DNA. Translation: AAC55064.1.

3D structure databases

SMRiP52283.
ModBaseiSearch...
MobiDBiSearch...

Protein family/group databases

REBASEi760. CviJI.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Family and domain databases

ProtoNetiSearch...

Entry informationi

Entry nameiT2C1_PBCVI
AccessioniPrimary (citable) accession number: P52283
Entry historyiIntegrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: February 15, 2017
This is version 45 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.