Skip Header

You are using a version of Internet Explorer that may not display all features of this website. Please upgrade to a modern browser.
Contribute Send feedback
Read comments (?) or add your own

P52283 (T2C1_PBCVI) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 43. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (1) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Type-2 restriction enzyme CviJI

Short name=R.CviJI
EC=3.1.21.4
Alternative name(s):
Endonuclease CviJI
Type II restriction enzyme CviJI
Gene names
Name:CVJIR
OrganismParamecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Taxonomic identifier46019 [NCBI]
Taxonomic lineageVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostChlorella [TaxID: 3071]

Protein attributes

Sequence length278 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Recognizes the double-stranded sequence RGCY and cleaves after G-2. In the presence of ATP, there is a relaxation of its specificity and it can cleave RGCN and YGCY, but not YGCR.

Catalytic activity

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactor

Magnesium.

Ontologies

Keywords
   Biological processRestriction system
   LigandMagnesium
   Molecular functionEndonuclease
Hydrolase
Nuclease
   Technical termDirect protein sequencing
Gene Ontology (GO)
   Biological_processDNA restriction-modification system

Inferred from electronic annotation. Source: UniProtKB-KW

   Molecular_functionType II site-specific deoxyribonuclease activity

Inferred from electronic annotation. Source: UniProtKB-EC

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 278278Type-2 restriction enzyme CviJI
PRO_0000077298

Sequences

Sequence LengthMass (Da)Tools
P52283 [UniParc].

Last modified October 1, 1996. Version 1.
Checksum: B348AD778C6A1EA8

FASTA27831,634
        10         20         30         40         50         60 
MEEKKRLALI EKQRIAEEKI ASGRKIRKRI STNATKHERE FVKVINSMFV GPATFVFVDI 

        70         80         90        100        110        120 
KGNKSREIHN VVRFRQLQGS KAKSPTAYVD REYNKPKADI AAVDITGKDV AWISHKASEG 

       130        140        150        160        170        180 
YQQYLKISGK NLKFTGKELE EVLSFKRKVV SMAPVSKIWP ANKTVWSPIK SNLIKNQAIF 

       190        200        210        220        230        240 
GFDYGKKPGR DNVDIIGQGR PIITKRGSIL YLTFTGFSAL NGHLENFTGK HEPVFYVRTE 

       250        260        270 
RSSSGRSITT VVNGVTYKNL RFFIHPYNFV SSKTQRIM 

« Hide

References

[1]"Cloning and applications of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella."
Skowron P.M., Swaminathan N., McMaster K., George D., van Etten J.L., Mead D.A.
Gene 157:37-41(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]"Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A."
Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M.
Nucleic Acids Res. 24:2463-2469(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
U09001 Genomic DNA. Translation: AAC55064.1.

3D structure databases

ModBaseSearch...
MobiDBSearch...

Protein family/group databases

REBASE760. CviJI.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Family and domain databases

ProtoNetSearch...

Entry information

Entry nameT2C1_PBCVI
AccessionPrimary (citable) accession number: P52283
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: April 16, 2014
This is version 43 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Relevant documents

Restriction enzymes and methylases

Classification of restriction enzymes and methylases and list of entries