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P52283

- T2C1_PBCVI

UniProt

P52283 - T2C1_PBCVI

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Protein

Type-2 restriction enzyme CviJI

Gene

CVJIR

Organism
Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli

Functioni

Recognizes the double-stranded sequence RGCY and cleaves after G-2. In the presence of ATP, there is a relaxation of its specificity and it can cleave RGCN and YGCY, but not YGCR.

Catalytic activityi

Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

Cofactori

GO - Molecular functioni

  1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

GO - Biological processi

  1. DNA restriction-modification system Source: UniProtKB-KW
Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

Restriction system

Keywords - Ligandi

Magnesium

Protein family/group databases

REBASEi760. CviJI.

Names & Taxonomyi

Protein namesi
Recommended name:
Type-2 restriction enzyme CviJI (EC:3.1.21.4)
Short name:
R.CviJI
Alternative name(s):
Endonuclease CviJI
Type II restriction enzyme CviJI
Gene namesi
Name:CVJIR
OrganismiParamecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Taxonomic identifieri46019 [NCBI]
Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
Virus hostiChlorella [TaxID: 3071]

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 278278Type-2 restriction enzyme CviJIPRO_0000077298Add
BLAST

Sequencei

Sequence statusi: Complete.

P52283-1 [UniParc]FASTAAdd to Basket

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        10         20         30         40         50
MEEKKRLALI EKQRIAEEKI ASGRKIRKRI STNATKHERE FVKVINSMFV
60 70 80 90 100
GPATFVFVDI KGNKSREIHN VVRFRQLQGS KAKSPTAYVD REYNKPKADI
110 120 130 140 150
AAVDITGKDV AWISHKASEG YQQYLKISGK NLKFTGKELE EVLSFKRKVV
160 170 180 190 200
SMAPVSKIWP ANKTVWSPIK SNLIKNQAIF GFDYGKKPGR DNVDIIGQGR
210 220 230 240 250
PIITKRGSIL YLTFTGFSAL NGHLENFTGK HEPVFYVRTE RSSSGRSITT
260 270
VVNGVTYKNL RFFIHPYNFV SSKTQRIM
Length:278
Mass (Da):31,634
Last modified:October 1, 1996 - v1
Checksum:iB348AD778C6A1EA8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09001 Genomic DNA. Translation: AAC55064.1.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
U09001 Genomic DNA. Translation: AAC55064.1 .

3D structure databases

ModBasei Search...
MobiDBi Search...

Protein family/group databases

REBASEi 760. CviJI.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Family and domain databases

ProtoNeti Search...

Publicationsi

  1. "Cloning and applications of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella."
    Skowron P.M., Swaminathan N., McMaster K., George D., van Etten J.L., Mead D.A.
    Gene 157:37-41(1995) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
  2. "Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A."
    Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M.
    Nucleic Acids Res. 24:2463-2469(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.

Entry informationi

Entry nameiT2C1_PBCVI
AccessioniPrimary (citable) accession number: P52283
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 26, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programViral Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Direct protein sequencing

Documents

  1. Restriction enzymes and methylases
    Classification of restriction enzymes and methylases and list of entries

External Data

Dasty 3