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P52283

- T2C1_PBCVI

UniProt

P52283 - T2C1_PBCVI

Protein

Type-2 restriction enzyme CviJI

Gene

CVJIR

Organism
Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)
Status
Reviewed - Annotation score: 2 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 43 (16 Apr 2014)
      Sequence version 1 (01 Oct 1996)
      Previous versions | rss
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    Functioni

    Recognizes the double-stranded sequence RGCY and cleaves after G-2. In the presence of ATP, there is a relaxation of its specificity and it can cleave RGCN and YGCY, but not YGCR.

    Catalytic activityi

    Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.

    Cofactori

    Magnesium.

    GO - Molecular functioni

    1. Type II site-specific deoxyribonuclease activity Source: UniProtKB-EC

    GO - Biological processi

    1. DNA restriction-modification system Source: UniProtKB-KW

    Keywords - Molecular functioni

    Endonuclease, Hydrolase, Nuclease

    Keywords - Biological processi

    Restriction system

    Keywords - Ligandi

    Magnesium

    Protein family/group databases

    REBASEi760. CviJI.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Type-2 restriction enzyme CviJI (EC:3.1.21.4)
    Short name:
    R.CviJI
    Alternative name(s):
    Endonuclease CviJI
    Type II restriction enzyme CviJI
    Gene namesi
    Name:CVJIR
    OrganismiParamecium bursaria Chlorella virus IL3A (PBCV-IL3A)
    Taxonomic identifieri46019 [NCBI]
    Taxonomic lineageiVirusesdsDNA viruses, no RNA stagePhycodnaviridaeChlorovirus
    Virus hostiChlorella [TaxID: 3071]

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 278278Type-2 restriction enzyme CviJIPRO_0000077298Add
    BLAST

    Sequencei

    Sequence statusi: Complete.

    P52283-1 [UniParc]FASTAAdd to Basket

    « Hide

    MEEKKRLALI EKQRIAEEKI ASGRKIRKRI STNATKHERE FVKVINSMFV    50
    GPATFVFVDI KGNKSREIHN VVRFRQLQGS KAKSPTAYVD REYNKPKADI 100
    AAVDITGKDV AWISHKASEG YQQYLKISGK NLKFTGKELE EVLSFKRKVV 150
    SMAPVSKIWP ANKTVWSPIK SNLIKNQAIF GFDYGKKPGR DNVDIIGQGR 200
    PIITKRGSIL YLTFTGFSAL NGHLENFTGK HEPVFYVRTE RSSSGRSITT 250
    VVNGVTYKNL RFFIHPYNFV SSKTQRIM 278
    Length:278
    Mass (Da):31,634
    Last modified:October 1, 1996 - v1
    Checksum:iB348AD778C6A1EA8
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09001 Genomic DNA. Translation: AAC55064.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    U09001 Genomic DNA. Translation: AAC55064.1 .

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    REBASEi 760. CviJI.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Family and domain databases

    ProtoNeti Search...

    Publicationsi

    1. "Cloning and applications of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella."
      Skowron P.M., Swaminathan N., McMaster K., George D., van Etten J.L., Mead D.A.
      Gene 157:37-41(1995) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
    2. "Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A."
      Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M.
      Nucleic Acids Res. 24:2463-2469(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.

    Entry informationi

    Entry nameiT2C1_PBCVI
    AccessioniPrimary (citable) accession number: P52283
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: October 1, 1996
    Last modified: April 16, 2014
    This is version 43 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programViral Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Direct protein sequencing

    Documents

    1. Restriction enzymes and methylases
      Classification of restriction enzymes and methylases and list of entries

    External Data

    Dasty 3