Reviewed, UniProtKB/Swiss-Prot P52283 (T2C1_PBCVI)

Names and origin

Protein names

Recommended name:
    Type-2 restriction enzyme CviJI
      Short name=R.CviJI
    EC=3.1.21.4
Alternative name(s):
    Type II restriction enzyme CviJI
    Endonuclease CviJI

Gene names

Name:

CVJIR

Organism

Paramecium bursaria Chlorella virus IL3A (PBCV-IL3A)

Taxonomic identifier

46019 [NCBI]

Taxonomic lineage

Viruses › dsDNA viruses, no RNA stage › Phycodnaviridae › Chlorovirus

Virus host

Chlorella [TaxID: 3071]

Protein attributes

Sequence length	278 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Recognizes the double-stranded sequence RGCY and cleaves after G-2. In the presence of ATP, there is a relaxation of its specificity and it can cleave RGCN and YGCY, but not YGCR.
Catalytic activity	Endonucleolytic cleavage of DNA to give specific double-stranded fragments with terminal 5'-phosphates.
Cofactor	Magnesium.

Ontologies

Keywords
Biological process	Restriction system
Ligand	Magnesium
Molecular function	Endonuclease Hydrolase Nuclease
Technical term	Direct protein sequencing
Gene Ontology (GO)
Biological process	DNA restriction-modification system Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	Type II site-specific deoxyribonuclease activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 278	278	Type-2 restriction enzyme CviJI		PRO_0000077298

Sequences

	Sequence		Length	Mass (Da)
	P52283-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: B348AD778C6A1EA8	FASTA	278	31,634
	10 20 30 40 50 60 MEEKKRLALI EKQRIAEEKI ASGRKIRKRI STNATKHERE FVKVINSMFV GPATFVFVDI 70 80 90 100 110 120 KGNKSREIHN VVRFRQLQGS KAKSPTAYVD REYNKPKADI AAVDITGKDV AWISHKASEG 130 140 150 160 170 180 YQQYLKISGK NLKFTGKELE EVLSFKRKVV SMAPVSKIWP ANKTVWSPIK SNLIKNQAIF 190 200 210 220 230 240 GFDYGKKPGR DNVDIIGQGR PIITKRGSIL YLTFTGFSAL NGHLENFTGK HEPVFYVRTE 250 260 270 RSSSGRSITT VVNGVTYKNL RFFIHPYNFV SSKTQRIM

References

[1]	"Cloning and applications of the two/three-base restriction endonuclease R.CviJI from IL-3A virus-infected Chlorella." Skowron P.M., Swaminathan N., McMaster K., George D., van Etten J.L., Mead D.A. Gene 157:37-41(1995) [PubMed: 7607522] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Molecular cloning of the three base restriction endonuclease R.CviJI from eukaryotic Chlorella virus IL-3A." Swaminathan N., Mead D.A., McMaster K., George D., van Etten J.L., Skowron P.M. Nucleic Acids Res. 24:2463-2469(1996) [PubMed: 8692682] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-15.

Cross-references

Sequence databases
EMBLGenBankDDBJ	U09001 Genomic DNA. Translation: AAC55064.1.
3D structure databases
ModBase	Search...
Protein family/group databases
REBASE	760. CviJI.
Enzyme and pathway databases
BRENDA	3.1.21.4. 291856.
Family and domain databases
ProtoNet	Search...

Entry information

Entry name

T2C1_PBCVI

Accession

Primary (citable) accession number: P52283

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	January 20, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

Virus (Virus annotation project)