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P52279

- PIP_XANCI

UniProt

P52279 - PIP_XANCI

Protein

Proline iminopeptidase

Gene

pip

Organism
Xanthomonas campestris pv. citri (Xanthomonas citri)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 81 (01 Oct 2014)
      Sequence version 2 (01 Nov 1997)
      Previous versions | rss
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    Functioni

    May be involved in proline metabolism and sensitivity to ascamycin. Has ascamycin dealanylating activity.

    Catalytic activityi

    Release of N-terminal proline from a peptide.

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Active sitei110 – 1101Nucleophile1 Publication
    Active sitei266 – 26611 Publication
    Active sitei294 – 2941Proton donor1 Publication

    GO - Molecular functioni

    1. aminopeptidase activity Source: UniProtKB-KW

    Keywords - Molecular functioni

    Aminopeptidase, Hydrolase, Protease

    Protein family/group databases

    MEROPSiS33.001.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Proline iminopeptidase (EC:3.4.11.5)
    Short name:
    PIP
    Alternative name(s):
    Prolyl aminopeptidase
    Short name:
    PAP
    Gene namesi
    Name:pip
    Synonyms:xap
    OrganismiXanthomonas campestris pv. citri (Xanthomonas citri)
    Taxonomic identifieri346 [NCBI]
    Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas

    Subcellular locationi

    Cytoplasm By similarity

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 313313Proline iminopeptidasePRO_0000080847Add
    BLAST

    Interactioni

    Subunit structurei

    Homooligomer.

    Structurei

    Secondary structure

    1
    313
    Legend: HelixTurnBeta strand
    Show more details
    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Beta strandi11 – 177
    Beta strandi19 – 213
    Beta strandi23 – 308
    Beta strandi34 – 407
    Turni43 – 464
    Helixi50 – 556
    Turni58 – 603
    Beta strandi61 – 666
    Beta strandi74 – 763
    Helixi85 – 9814
    Beta strandi102 – 1098
    Helixi111 – 12212
    Helixi124 – 1263
    Beta strandi127 – 1348
    Helixi140 – 1478
    Helixi151 – 1544
    Helixi156 – 1649
    Helixi168 – 1703
    Helixi174 – 1829
    Helixi187 – 20317
    Beta strandi205 – 2084
    Helixi211 – 2177
    Helixi220 – 23516
    Helixi237 – 2393
    Helixi245 – 2484
    Helixi250 – 2534
    Beta strandi258 – 2636
    Beta strandi267 – 2693
    Helixi271 – 28010
    Beta strandi284 – 2896
    Helixi299 – 31214

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    EntryMethodResolution (Å)ChainPositionsPDBsum
    1AZWX-ray2.70A/B1-313[»]
    ProteinModelPortaliP52279.
    SMRiP52279. Positions 1-313.
    ModBaseiSearch...
    MobiDBiSearch...

    Miscellaneous databases

    EvolutionaryTraceiP52279.

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the peptidase S33 family.Curated

    Family and domain databases

    Gene3Di3.40.50.1820. 1 hit.
    InterProiIPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR002410. Peptidase_S33.
    IPR005944. Pro_iminopeptidase.
    [Graphical view]
    PfamiPF00561. Abhydrolase_1. 1 hit.
    [Graphical view]
    PIRSFiPIRSF006431. Pept_S33. 1 hit.
    PRINTSiPR00793. PROAMNOPTASE.
    SUPFAMiSSF53474. SSF53474. 1 hit.
    TIGRFAMsiTIGR01249. pro_imino_pep_1. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    P52279-1 [UniParc]FASTAAdd to Basket

    « Hide

    MRTLYPEITP YQQGSLKVDD RHTLYFEQCG NPHGKPVVML HGGPGGGCND    50
    KMRRFHDPAK YRIVLFDQRG SGRSTPHADL VDNTTWDLVA DIERLRTHLG 100
    VDRWQVFGGS WGSTLALAYA ADPSAAGHQL VLRGIFLLRR FELEWFYQEG 150
    ASRLFPDAWE HYLNAIPPVE RADLMSAFHR RLTSDDEATR LAAAKAWSVW 200
    EGATSFLHVD EDFVTGHEDA HFALAFARIE NHYFVNGGFF EVEDQLLRDA 250
    HRIADIPGVI VHGRYDVVCP LQSAWDLHKA WPKAQLQISP ASGHSAFEPE 300
    NVDALVRATD GFA 313
    Length:313
    Mass (Da):35,260
    Last modified:November 1, 1997 - v2
    Checksum:i7A9DF2636363FF48
    GO

    Experimental Info

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sequence conflicti95 – 951L → V in BAA11623. (PubMed:8870654)Curated
    Sequence conflicti121 – 1299ADPSAAGHQ → QTHPQQVTE in BAA11623. (PubMed:8870654)Curated
    Sequence conflicti245 – 2517QLLRDAH → SCCATD in BAA11623. (PubMed:8870654)Curated
    Sequence conflicti284 – 2841Missing in BAA11623. (PubMed:8870654)Curated

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54150 Genomic DNA. Translation: CAA90864.1.
    D82882 Genomic DNA. Translation: BAA11623.1.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    Z54150 Genomic DNA. Translation: CAA90864.1 .
    D82882 Genomic DNA. Translation: BAA11623.1 .

    3D structure databases

    Select the link destinations:
    PDBe
    RCSB PDB
    PDBj
    Links Updated
    Entry Method Resolution (Å) Chain Positions PDBsum
    1AZW X-ray 2.70 A/B 1-313 [» ]
    ProteinModelPortali P52279.
    SMRi P52279. Positions 1-313.
    ModBasei Search...
    MobiDBi Search...

    Protein family/group databases

    MEROPSi S33.001.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Miscellaneous databases

    EvolutionaryTracei P52279.

    Family and domain databases

    Gene3Di 3.40.50.1820. 1 hit.
    InterProi IPR029058. AB_hydrolase.
    IPR000073. AB_hydrolase_1.
    IPR002410. Peptidase_S33.
    IPR005944. Pro_iminopeptidase.
    [Graphical view ]
    Pfami PF00561. Abhydrolase_1. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF006431. Pept_S33. 1 hit.
    PRINTSi PR00793. PROAMNOPTASE.
    SUPFAMi SSF53474. SSF53474. 1 hit.
    TIGRFAMsi TIGR01249. pro_imino_pep_1. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. "Proline iminopeptidase gene from Xanthomonas campestris pv. citri."
      Alonso J., Garcia J.L.
      Microbiology 142:2951-2957(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
      Strain: NBRC 3835.
    2. Alonso J.
      Submitted (FEB-1997) to the EMBL/GenBank/DDBJ databases
      Cited for: SEQUENCE REVISION.
    3. "Isolation and characterization of the gene encoding an aminopeptidase involved in the selective toxicity of ascamycin toward Xanthomonas campestris pv. citri."
      Sudo T., Shinohara K., Dohmae N., Takio K., Usami R., Horikoshi K., Osada H.
      Biochem. J. 319:99-102(1996) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-20 AND 283-296.
      Strain: NBRC 3781.
    4. "Structure of proline iminopeptidase from Xanthomonas campestris pv. citri: a prototype for the prolyl oligopeptidase family."
      Medrano F.J., Alonso J., Garcia J.L., Romero A., Bode W., Gomis-Ruth F.X.
      EMBO J. 17:1-9(1998) [PubMed] [Europe PMC] [Abstract]
      Cited for: X-RAY CRYSTALLOGRAPHY (2.7 ANGSTROMS), ACTIVE SITE.

    Entry informationi

    Entry nameiPIP_XANCI
    AccessioniPrimary (citable) accession number: P52279
    Secondary accession number(s): P96186
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: October 1, 1996
    Last sequence update: November 1, 1997
    Last modified: October 1, 2014
    This is version 81 of the entry and version 2 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    3D-structure, Direct protein sequencing

    Documents

    1. PDB cross-references
      Index of Protein Data Bank (PDB) cross-references
    2. Peptidase families
      Classification of peptidase families and list of entries
    3. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3