Reviewed, UniProtKB/Swiss-Prot P52278 (PIP_LACHE)

Names and origin

Protein names

Recommended name:
    Proline iminopeptidase
      Short name=PIP
    EC=3.4.11.5
Alternative name(s):
    Prolyl aminopeptidase
      Short name=PAP

Gene names

Name:	pip
Synonyms:	pepI

Organism

Lactobacillus helveticus

Taxonomic identifier

1587 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Lactobacillales › Lactobacillaceae › Lactobacillus

Protein attributes

Sequence length	294 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	Release of N-terminal proline from a peptide.
Subunit structure	Homotrimer By similarity.
Subcellular location	Cell envelope Potential.
Sequence similarities	Belongs to the peptidase S33 family.

Ontologies

Keywords
Molecular function	Aminopeptidase Hydrolase Protease
Gene Ontology (GO)
Biological process	proteolysis Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: InterPro envelope Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	aminopeptidase activity Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 294	294	Proline iminopeptidase		PRO_0000080838
Sites
	Active site	105	1	Nucleophile By similarity
	Active site	244	1	By similarity
	Active site	271	1	Proton donor By similarity

Sequences

	Sequence		Length	Mass (Da)
	P52278-1 [UniParc]. Last modified October 1, 1996. Version 1. Checksum: 535042DAFD636979	FASTA	294	33,845
	10 20 30 40 50 60 MEIIEGKMPF MGYETYYRIV GERSEKPPLV LLHGGPGSSH NYFEVLDELA QKDGRRIIMY 70 80 90 100 110 120 DQLGCGESSI PDDHPELYTK ETWVKELEAL REHLALRKMH LLGQSWGGML AIIYMCDYHP 130 140 150 160 170 180 EGIQSLILSS TLSSASLWSK ELHRMIKYLP IEEQAAIHRA ELTGNFNDPD YLKANEHFMN 190 200 210 220 230 240 QHAIDMTKTW PECVMRKKRG GTVAYETAWG PNEYTPEGNL HDYEYTDKLS KIKVPTLITS 250 260 270 280 290 GTDDLCTPYV AKTMQDQIAS SKWRLFEGCG HMSFVEKTDE YVALLQEWLD QHDE

References

[1]

"An operon from Lactobacillus helveticus composed of a proline iminopeptidase gene (pepI) and two genes coding for putative members of the ABC transporter family of proteins." Varmanen P., Rantanen T., Palva A. Microbiology 142:3459-3468(1996) [PubMed: 9004508] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: 53/7.

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z56283 Genomic DNA. Translation: CAA91231.1.
3D structure databases
HSSP	HSSP built from PDB template 1MTZ based on UniProtKB P96084.
ModBase	Search...
Enzyme and pathway databases
BRENDA	3.4.11.5. 39238.
Family and domain databases
InterPro	IPR000073. AB_hydrolase_1. IPR005945. Pept_S33_TRI_F1. IPR002410. Peptidase_S33. [Graphical view]
Pfam	PF00561. Abhydrolase_1. 1 hit. [Graphical view]
PIRSF	PIRSF005539. Pept_S33_TRI_F1. 1 hit.
PRINTS	PR00793. PROAMNOPTASE.
TIGRFAMs	TIGR01250. pro_imino_pep_2. 1 hit.
ProtoNet	Search...

Entry information

Entry name

PIP_LACHE

Accession

Primary (citable) accession number: P52278

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	October 1, 1996
Last modified:	June 16, 2009
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)