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P52276 (SYN_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 101. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (3) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Asparagine--tRNA ligase

EC=6.1.1.22
Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS
Gene names
Name:asnS
Ordered Locus Names:sll0495
OrganismSynechocystis sp. (strain PCC 6803 / Kazusa) [Reference proteome] [HAMAP]
Taxonomic identifier1111708 [NCBI]
Taxonomic lineageBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechocystis

Protein attributes

Sequence length462 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP-Rule MF_00534

Subunit structure

Homodimer By similarity. HAMAP-Rule MF_00534

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00534.

Sequence similarities

Belongs to the class-II aminoacyl-tRNA synthetase family.

Sequence caution

The sequence BAA10844.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Reference proteome
Gene Ontology (GO)
   Biological_processasparaginyl-tRNA aminoacylation

Inferred from electronic annotation. Source: InterPro

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-KW

asparagine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-EC

nucleic acid binding

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 462462Asparagine--tRNA ligase HAMAP-Rule MF_00534
PRO_0000176468

Sequences

Sequence LengthMass (Da)Tools
P52276 [UniParc].

Last modified January 31, 2002. Version 2.
Checksum: 6E177B9BC0F25D1F

FASTA46252,699
        10         20         30         40         50         60 
MNKRRIIEVL RHGQADDQVM VQGWLRTKRT LKDFSFVEVN DGSSLANLQV VLDGSLADYD 

        70         80         90        100        110        120 
RLLSQLQTGA ALVVEGKLAP SPGKGQRVEL KATKLELLGG ADPGSYPLQK KRHSFEFLRT 

       130        140        150        160        170        180 
IGHLRPRTNT IGAVMRVRNA CATAIHQFFQ ERGFLWVHTP IITASDCEGA GDLFKVTTLD 

       190        200        210        220        230        240 
LQRVPKNGEG IDYSQDFFGK QAYLTVSGQL EAEVMALAFQ NVYTFGPTFR AENSNTSRHL 

       250        260        270        280        290        300 
AEFWMVEPEM AFCDLEGDRQ WAEEFLKYIF KFVLEKCPED MEFFDQRIDN TVLATADNII 

       310        320        330        340        350        360 
NNEFAWLTYS EAIKLLEKAD QKFEYPVAWG VDLQSEHERY LAETVFKRPT IVTDYPKDIK 

       370        380        390        400        410        420 
AFYMRLNDDG KTVAAMDILA PKIGEIIGGS QREERLDILT QRMQEQGVPE EDLWWYLDLR 

       430        440        450        460 
RYGSVPHAGF GLGFERIVQF MTGMANIRDV IPFPRTPMNA EF 

« Hide

References

[1]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome."
Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S.
DNA Res. 2:153-166(1995) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 27184 / PCC 6803 / N-1.
[2]"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions."
Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. expand/collapse author list , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S.
DNA Res. 3:109-136(1996) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: PCC 6803 / Kazusa.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BA000022 Genomic DNA. Translation: BAA10844.1. Different initiation.
PIRS75997.
RefSeqYP_007452649.1. NC_020286.1.

3D structure databases

ProteinModelPortalP52276.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

IntActP52276. 1 interaction.
STRING1148.sll0495.

Proteomic databases

PaxDbP52276.
PRIDEP52276.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaBAA10844; BAA10844; BAA10844.
GeneID14618331.
KEGGsyz:MYO_129070.
PATRIC23843302. VBISynSp132158_3189.

Phylogenomic databases

eggNOGCOG0017.
HOGENOMHOG000226033.
KOK01893.
OMAAIHRFFH.
PhylomeDBP52276.
ProtClustDBPRK03932.

Family and domain databases

Gene3D2.40.50.140. 1 hit.
HAMAPMF_00534. Asn_tRNA_synth.
InterProIPR004364. aa-tRNA-synt_II.
IPR018150. aa-tRNA-synt_II-like.
IPR006195. aa-tRNA-synth_II.
IPR004522. Asn-tRNA-ligase.
IPR002312. Asp/Asn-tRNA-synth_IIb.
IPR012340. NA-bd_OB-fold.
IPR004365. NA-bd_OB_tRNA.
[Graphical view]
PANTHERPTHR22594. PTHR22594. 1 hit.
PTHR22594:SF6. PTHR22594:SF6. 1 hit.
PfamPF00152. tRNA-synt_2. 1 hit.
PF01336. tRNA_anti-codon. 1 hit.
[Graphical view]
PRINTSPR01042. TRNASYNTHASP.
SUPFAMSSF50249. SSF50249. 1 hit.
TIGRFAMsTIGR00457. asnS. 1 hit.
PROSITEPS50862. AA_TRNA_LIGASE_II. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYN_SYNY3
AccessionPrimary (citable) accession number: P52276
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 31, 2002
Last modified: April 16, 2014
This is version 101 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

Synechocystis PCC 6803

Synechocystis (strain PCC 6803): entries and gene names

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries