P52276 (SYN_SYNY3) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Asparagine--tRNA ligase
EC=6.1.1.22

Alternative name(s):
Asparaginyl-tRNA synthetase
Short name=AsnRS

Gene names

Name:	asnS
Ordered Locus Names:	sll0495

Organism

Synechocystis sp. (strain PCC 6803 / Kazusa)

Taxonomic identifier

1111708 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechocystis

Protein attributes

Sequence length	462 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + L-asparaginyl-tRNA(Asn). HAMAP MF_00534
Subunit structure	Homodimer By similarity. HAMAP MF_00534
Subcellular location	Cytoplasm HAMAP MF_00534.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family.
Sequence caution	The sequence BAA10844.1 differs from that shown. Reason: Erroneous initiation.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological process	asparaginyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW asparagine-tRNA ligase activity Inferred from electronic annotation. Source: EC nucleic acid binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 462	462	Asparagine--tRNA ligase HAMAP MF_00534		PRO_0000176468

Sequences

	Sequence		Length	Mass (Da)
	P52276 [UniParc]. Last modified January 31, 2002. Version 2. Checksum: 6E177B9BC0F25D1F	FASTA	462	52,699
	10 20 30 40 50 60 MNKRRIIEVL RHGQADDQVM VQGWLRTKRT LKDFSFVEVN DGSSLANLQV VLDGSLADYD 70 80 90 100 110 120 RLLSQLQTGA ALVVEGKLAP SPGKGQRVEL KATKLELLGG ADPGSYPLQK KRHSFEFLRT 130 140 150 160 170 180 IGHLRPRTNT IGAVMRVRNA CATAIHQFFQ ERGFLWVHTP IITASDCEGA GDLFKVTTLD 190 200 210 220 230 240 LQRVPKNGEG IDYSQDFFGK QAYLTVSGQL EAEVMALAFQ NVYTFGPTFR AENSNTSRHL 250 260 270 280 290 300 AEFWMVEPEM AFCDLEGDRQ WAEEFLKYIF KFVLEKCPED MEFFDQRIDN TVLATADNII 310 320 330 340 350 360 NNEFAWLTYS EAIKLLEKAD QKFEYPVAWG VDLQSEHERY LAETVFKRPT IVTDYPKDIK 370 380 390 400 410 420 AFYMRLNDDG KTVAAMDILA PKIGEIIGGS QREERLDILT QRMQEQGVPE EDLWWYLDLR 430 440 450 460 RYGSVPHAGF GLGFERIVQF MTGMANIRDV IPFPRTPMNA EF

References

[1]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. I. Sequence features in the 1 Mb region from map positions 64% to 92% of the genome." Kaneko T., Tanaka A., Sato S., Kotani H., Sazuka T., Miyajima N., Sugiura M., Tabata S. DNA Res. 2:153-166(1995) [PubMed: 8590279] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
[2]	"Sequence analysis of the genome of the unicellular cyanobacterium Synechocystis sp. strain PCC6803. II. Sequence determination of the entire genome and assignment of potential protein-coding regions." Kaneko T., Sato S., Kotani H., Tanaka A., Asamizu E., Nakamura Y., Miyajima N., Hirosawa M., Sugiura M., Sasamoto S., Kimura T., Hosouchi T., Matsuno A., Muraki A., Nakazaki N., Naruo K., Okumura S., Shimpo S. , Takeuchi C., Wada T., Watanabe A., Yamada M., Yasuda M., Tabata S. DNA Res. 3:109-136(1996) [PubMed: 8905231] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 27184 / PCC 6803 / N-1.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BA000022 Genomic DNA. Translation: BAA10844.1. Different initiation.
PIR	S75997.
RefSeq	NP_442773.2. NC_000911.1.
3D structure databases
ProteinModelPortal	P52276.
ModBase	Search...
Protein-protein interaction databases
IntAct	P52276. 1 interaction.
STRING	P52276.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	952103.
GenomeReviews	Gene locus sll0495 in contig BA000022_GR.
KEGG	syn:sll0495.
NMPDR	fig|1148.1.peg.2874.
PATRIC	23843302. VBISynSp132158_3189.
Phylogenomic databases
eggNOG	COG0017.
HOGENOM	HBG745843.
OMA	AIHRFFH.
PhylomeDB	P52276.
ProtClustDB	PRK03932.
Enzyme and pathway databases
BioCyc	SSP1148:SLL0495-MONOMER.
Family and domain databases
HAMAP	MF_00534. Asn_tRNA_synth. [Tree]
InterPro	IPR004364. aa-tRNA-synt_II. IPR018150. aa-tRNA-synt_II-like. IPR006195. aa-tRNA-synth_II. IPR004522. Asn-tRNA-synth_IIb. IPR002312. Asp/Asn-tRNA-synth_IIb. IPR012340. NA-bd_OB-fold. IPR016027. NA-bd_OB-fold-like. IPR004365. NA-bd_OB_tRNA-helicase. [Graphical view]
Gene3D	G3DSA:2.40.50.140. OB_NA_bd_sub. 1 hit.
KO	K01893.
PANTHER	PTHR22594. aa-tRNA-synt_II. 1 hit. PTHR22594:SF6. PTHR22594:SF6. 1 hit.
Pfam	PF00152. tRNA-synt_2. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
PRINTS	PR01042. TRNASYNTHASP.
SUPFAM	SSF50249. Nucleic_acid_OB. 1 hit.
TIGRFAMs	TIGR00457. AsnS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYN_SYNY3

Accession

Primary (citable) accession number: P52276

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 31, 2002
Last modified:	January 25, 2012
This is version 85 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program