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Protein

Heterogeneous nuclear ribonucleoprotein M

Gene

HNRNPM

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Pre-mRNA binding protein in vivo, binds avidly to poly(G) and poly(U) RNA homopolymers in vitro. Involved in splicing. Acts as a receptor for carcinoembryonic antigen in Kupffer cells, may initiate a series of signaling events leading to tyrosine phosphorylation of proteins and induction of IL-1 alpha, IL-6, IL-10 and tumor necrosis factor alpha cytokines.

GO - Molecular functioni

  • nucleotide binding Source: InterPro
  • poly(A) RNA binding Source: UniProtKB
  • protein domain specific binding Source: UniProtKB
  • RNA binding Source: HGNC

GO - Biological processi

  • alternative mRNA splicing, via spliceosome Source: BHF-UCL
  • fibroblast growth factor receptor signaling pathway Source: Reactome
  • gene expression Source: Reactome
  • mRNA splicing, via spliceosome Source: UniProtKB
Complete GO annotation...

Keywords - Molecular functioni

Ribonucleoprotein

Keywords - Biological processi

mRNA processing, mRNA splicing

Keywords - Ligandi

RNA-binding

Enzyme and pathway databases

BioCyciZFISH:ENSG00000099783-MONOMER.
ReactomeiR-HSA-6803529. FGFR2 alternative splicing.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
SIGNORiP52272.

Names & Taxonomyi

Protein namesi
Recommended name:
Heterogeneous nuclear ribonucleoprotein M
Short name:
hnRNP M
Gene namesi
Name:HNRNPM
Synonyms:HNRPM, NAGR1
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 19

Organism-specific databases

HGNCiHGNC:5046. HNRNPM.

Subcellular locationi

  • Nucleusnucleolus 1 Publication

GO - Cellular componenti

  • catalytic step 2 spliceosome Source: UniProtKB
  • extracellular exosome Source: UniProtKB
  • extracellular matrix Source: UniProtKB
  • integral component of plasma membrane Source: ProtInc
  • membrane Source: UniProtKB
  • nuclear matrix Source: BHF-UCL
  • nucleolus Source: UniProtKB-SubCell
  • nucleoplasm Source: BHF-UCL
  • paraspeckles Source: BHF-UCL
  • spliceosomal complex Source: HGNC
Complete GO annotation...

Keywords - Cellular componenti

Nucleus, Spliceosome

Pathology & Biotechi

Organism-specific databases

DisGeNETi4670.
OpenTargetsiENSG00000099783.
PharmGKBiPA29370.

Polymorphism and mutation databases

BioMutaiHNRNPM.
DMDMi55977747.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Initiator methionineiRemovedCombined sources1 Publication
ChainiPRO_00000818642 – 730Heterogeneous nuclear ribonucleoprotein MAdd BLAST729

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei2N-acetylalanineCombined sources1 Publication1
Cross-linki17Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei29PhosphoserineCombined sources1
Cross-linki83Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei86PhosphoserineCombined sources1
Modified residuei134N6-acetyllysineBy similarity1
Cross-linki145Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei204PhosphoserineCombined sources1
Modified residuei277N6-acetyllysineCombined sources1
Modified residuei365PhosphoserineCombined sources1
Modified residuei377PhosphoserineCombined sources1
Cross-linki388Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei397PhosphoserineCombined sources1
Modified residuei432PhosphoserineCombined sources1
Modified residuei452PhosphoserineCombined sources1
Modified residuei468PhosphoserineCombined sources1
Modified residuei481PhosphoserineCombined sources1
Modified residuei496Omega-N-methylarginineCombined sources1
Modified residuei528PhosphoserineCombined sources1
Modified residuei575PhosphoserineCombined sources1
Modified residuei588PhosphoserineCombined sources1
Modified residuei618PhosphoserineCombined sources1
Modified residuei633PhosphoserineCombined sources1
Modified residuei637PhosphoserineCombined sources1
Modified residuei665PhosphothreonineCombined sources1
Modified residuei672N6-acetyllysineBy similarity1
Cross-linki685Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2)Combined sources
Modified residuei698N6-acetyllysine; alternateCombined sources1
Cross-linki698Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO1); alternateCombined sources
Cross-linki698Glycyl lysine isopeptide (Lys-Gly) (interchain with G-Cter in SUMO2); alternateCombined sources
Modified residuei701PhosphoserineCombined sources1

Post-translational modificationi

Sumoylated.1 Publication

Keywords - PTMi

Acetylation, Isopeptide bond, Methylation, Phosphoprotein, Ubl conjugation

Proteomic databases

EPDiP52272.
PaxDbiP52272.
PeptideAtlasiP52272.
PRIDEiP52272.

2D gel databases

REPRODUCTION-2DPAGEIPI00383296.
UCD-2DPAGEP52272.

PTM databases

iPTMnetiP52272.
PhosphoSitePlusiP52272.
SwissPalmiP52272.

Expressioni

Gene expression databases

BgeeiENSG00000099783.
CleanExiHS_HNRNPM.
ExpressionAtlasiP52272. baseline and differential.
GenevisibleiP52272. HS.

Organism-specific databases

HPAiCAB016113.
HPA024344.

Interactioni

Subunit structurei

Identified in the spliceosome C complex.1 Publication

Binary interactionsi

WithEntry#Exp.IntActNotes
AKAP9Q99996-23EBI-486809,EBI-9641546
CDC5LQ994597EBI-486809,EBI-374880
EBNA-LPQ8AZK72EBI-486809,EBI-1185167From a different organism.
GMCL1P1Q8NEA95EBI-486809,EBI-745707
HNRNPFP525973EBI-486809,EBI-352986
LMO2P257914EBI-486809,EBI-739696
PLRG1O436605EBI-486809,EBI-1051504
TEKT4Q8WW243EBI-486809,EBI-750487

GO - Molecular functioni

  • protein domain specific binding Source: UniProtKB

Protein-protein interaction databases

BioGridi110751. 251 interactors.
DIPiDIP-29336N.
IntActiP52272. 73 interactors.
MINTiMINT-4999200.
STRINGi9606.ENSP00000325376.

Structurei

Secondary structure

1730
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Beta strandi206 – 210Combined sources5
Helixi217 – 224Combined sources8
Turni225 – 227Combined sources3
Beta strandi230 – 237Combined sources8
Beta strandi242 – 253Combined sources12
Helixi254 – 264Combined sources11
Beta strandi275 – 278Combined sources4
Beta strandi654 – 657Combined sources4
Helixi666 – 674Combined sources9
Beta strandi679 – 689Combined sources11
Beta strandi691 – 701Combined sources11
Helixi702 – 712Combined sources11
Beta strandi725 – 727Combined sources3

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DGVNMR-A652-730[»]
2DH9NMR-A655-730[»]
2DO0NMR-A196-296[»]
2OT8X-ray3.10C/D41-70[»]
ProteinModelPortaliP52272.
SMRiP52272.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52272.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini71 – 149RRM 1PROSITE-ProRule annotationAdd BLAST79
Domaini204 – 281RRM 2PROSITE-ProRule annotationAdd BLAST78
Repeati400 – 40516
Repeati407 – 41226
Repeati415 – 42036
Repeati426 – 43146
Repeati433 – 43856
Repeati440 – 44566
Repeati446 – 45176
Repeati453 – 45886
Repeati461 – 46696
Repeati468 – 473106
Repeati475 – 480116
Repeati482 – 487126
Repeati493 – 498136
Repeati500 – 505146
Repeati507 – 512156
Repeati514 – 519166
Repeati521 – 526176
Repeati528 – 533186
Repeati540 – 545196
Repeati547 – 552206
Repeati554 – 559216
Repeati562 – 567226
Repeati567 – 572236
Repeati575 – 580246
Repeati580 – 585256
Repeati588 – 593266
Repeati603 – 608276
Domaini653 – 729RRM 3PROSITE-ProRule annotationAdd BLAST77

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni400 – 60827 X 6 AA repeats of [GEVSTPAN]-[ILMV]-[DE]-[RH]-[MLVI]-[GAV]Add BLAST209

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi390 – 396Poly-Gly7
Compositional biasi612 – 616Poly-Gly5

Sequence similaritiesi

Contains 3 RRM (RNA recognition motif) domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiKOG4212. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00410000025635.
HOGENOMiHOG000231932.
HOVERGENiHBG054880.
InParanoidiP52272.
KOiK12887.
OMAiGMNKMGG.
PhylomeDBiP52272.
TreeFamiTF313406.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR024667. HnRNP_M.
IPR024666. HnRNP_M_PY-NLS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23003:SF6. PTHR23003:SF6. 3 hits.
PfamiPF11532. HnRNP_M. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]

Sequences (3)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 3 isoformsi produced by alternative splicing. AlignAdd to basket

Note: Experimental confirmation may be lacking for some isoforms.
Isoform 1 (identifier: P52272-1) [UniParc]FASTAAdd to basket
Also known as: M4

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MAAGVEAAAE VAATEIKMEE ESGAPGVPSG NGAPGPKGEG ERPAQNEKRK
60 70 80 90 100
EKNIKRGGNR FEPYANPTKR YRAFITNIPF DVKWQSLKDL VKEKVGEVTY
110 120 130 140 150
VELLMDAEGK SRGCAVVEFK MEESMKKAAE VLNKHSLSGR PLKVKEDPDG
160 170 180 190 200
EHARRAMQKV MATTGGMGMG PGGPGMITIP PSILNNPNIP NEIIHALQAG
210 220 230 240 250
RLGSTVFVAN LDYKVGWKKL KEVFSMAGVV VRADILEDKD GKSRGIGTVT
260 270 280 290 300
FEQSIEAVQA ISMFNGQLLF DRPMHVKMDE RALPKGDFFP PERPQQLPHG
310 320 330 340 350
LGGIGMGLGP GGQPIDANHL NKGIGMGNIG PAGMGMEGIG FGINKMGGME
360 370 380 390 400
GPFGGGMENM GRFGSGMNMG RINEILSNAL KRGEIIAKQG GGGGGGSVPG
410 420 430 440 450
IERMGPGIDR LGGAGMERMG AGLGHGMDRV GSEIERMGLV MDRMGSVERM
460 470 480 490 500
GSGIERMGPL GLDHMASSIE RMGQTMERIG SGVERMGAGM GFGLERMAAP
510 520 530 540 550
IDRVGQTIER MGSGVERMGP AIERMGLSME RMVPAGMGAG LERMGPVMDR
560 570 580 590 600
MATGLERMGA NNLERMGLER MGANSLERMG LERMGANSLE RMGPAMGPAL
610 620 630 640 650
GAGIERMGLA MGGGGGASFD RAIEMERGNF GGSFAGSFGG AGGHAPGVAR
660 670 680 690 700
KACQIFVRNL PFDFTWKMLK DKFNECGHVL YADIKMENGK SKGCGVVKFE
710 720 730
SPEVAERACR MMNGMKLSGR EIDVRIDRNA
Length:730
Mass (Da):77,516
Last modified:January 23, 2007 - v3
Checksum:i1A73DD35A3501861
GO
Isoform 2 (identifier: P52272-2) [UniParc]FASTAAdd to basket
Also known as: M1-M2

The sequence of this isoform differs from the canonical sequence as follows:
     160-198: Missing.

Show »
Length:691
Mass (Da):73,621
Checksum:i028646C136D3CBD1
GO
Isoform 3 (identifier: P52272-3)
Also known as: M3
Sequence is not available
Length:
Mass (Da):

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti24 – 34APGVPSGNGAP → GPACERQRGS in AAA36192 (PubMed:8441656).CuratedAdd BLAST11
Sequence conflicti34P → S in AAC16002 (Ref. 3) Curated1
Sequence conflicti51E → V in AAA36192 (PubMed:8441656).Curated1
Sequence conflicti152H → C in AAC16002 (Ref. 3) Curated1
Sequence conflicti527L → P (PubMed:8441656).Curated1
Sequence conflicti527L → P (Ref. 3) Curated1

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_005845160 – 198Missing in isoform 2. 3 PublicationsAdd BLAST39

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03532 mRNA. Translation: AAA36192.1.
L32611 mRNA. Translation: AAL31359.1.
AF061832 mRNA. Translation: AAC16002.1.
BC000138 mRNA. Translation: AAH00138.2.
BC008895 mRNA. Translation: AAH08895.2.
BC019580 mRNA. Translation: AAH19580.1.
CCDSiCCDS12203.1. [P52272-1]
CCDS12204.1. [P52272-2]
PIRiS35532.
RefSeqiNP_005959.2. NM_005968.4. [P52272-1]
NP_112480.2. NM_031203.3. [P52272-2]
UniGeneiHs.465808.
Hs.666214.

Genome annotation databases

EnsembliENST00000325495; ENSP00000325376; ENSG00000099783. [P52272-1]
ENST00000348943; ENSP00000325732; ENSG00000099783. [P52272-2]
GeneIDi4670.
KEGGihsa:4670.
UCSCiuc010dwd.4. human. [P52272-1]

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L03532 mRNA. Translation: AAA36192.1.
L32611 mRNA. Translation: AAL31359.1.
AF061832 mRNA. Translation: AAC16002.1.
BC000138 mRNA. Translation: AAH00138.2.
BC008895 mRNA. Translation: AAH08895.2.
BC019580 mRNA. Translation: AAH19580.1.
CCDSiCCDS12203.1. [P52272-1]
CCDS12204.1. [P52272-2]
PIRiS35532.
RefSeqiNP_005959.2. NM_005968.4. [P52272-1]
NP_112480.2. NM_031203.3. [P52272-2]
UniGeneiHs.465808.
Hs.666214.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
2DGVNMR-A652-730[»]
2DH9NMR-A655-730[»]
2DO0NMR-A196-296[»]
2OT8X-ray3.10C/D41-70[»]
ProteinModelPortaliP52272.
SMRiP52272.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi110751. 251 interactors.
DIPiDIP-29336N.
IntActiP52272. 73 interactors.
MINTiMINT-4999200.
STRINGi9606.ENSP00000325376.

PTM databases

iPTMnetiP52272.
PhosphoSitePlusiP52272.
SwissPalmiP52272.

Polymorphism and mutation databases

BioMutaiHNRNPM.
DMDMi55977747.

2D gel databases

REPRODUCTION-2DPAGEIPI00383296.
UCD-2DPAGEP52272.

Proteomic databases

EPDiP52272.
PaxDbiP52272.
PeptideAtlasiP52272.
PRIDEiP52272.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000325495; ENSP00000325376; ENSG00000099783. [P52272-1]
ENST00000348943; ENSP00000325732; ENSG00000099783. [P52272-2]
GeneIDi4670.
KEGGihsa:4670.
UCSCiuc010dwd.4. human. [P52272-1]

Organism-specific databases

CTDi4670.
DisGeNETi4670.
GeneCardsiHNRNPM.
H-InvDBHIX0137462.
HGNCiHGNC:5046. HNRNPM.
HPAiCAB016113.
HPA024344.
MIMi160994. gene.
neXtProtiNX_P52272.
OpenTargetsiENSG00000099783.
PharmGKBiPA29370.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4212. Eukaryota.
COG0724. LUCA.
GeneTreeiENSGT00410000025635.
HOGENOMiHOG000231932.
HOVERGENiHBG054880.
InParanoidiP52272.
KOiK12887.
OMAiGMNKMGG.
PhylomeDBiP52272.
TreeFamiTF313406.

Enzyme and pathway databases

BioCyciZFISH:ENSG00000099783-MONOMER.
ReactomeiR-HSA-6803529. FGFR2 alternative splicing.
R-HSA-72163. mRNA Splicing - Major Pathway.
R-HSA-72203. Processing of Capped Intron-Containing Pre-mRNA.
SIGNORiP52272.

Miscellaneous databases

ChiTaRSiHNRNPM. human.
EvolutionaryTraceiP52272.
GenomeRNAii4670.
PROiP52272.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000099783.
CleanExiHS_HNRNPM.
ExpressionAtlasiP52272. baseline and differential.
GenevisibleiP52272. HS.

Family and domain databases

Gene3Di3.30.70.330. 3 hits.
InterProiIPR024667. HnRNP_M.
IPR024666. HnRNP_M_PY-NLS.
IPR012677. Nucleotide-bd_a/b_plait.
IPR000504. RRM_dom.
[Graphical view]
PANTHERiPTHR23003:SF6. PTHR23003:SF6. 3 hits.
PfamiPF11532. HnRNP_M. 1 hit.
PF00076. RRM_1. 3 hits.
[Graphical view]
SMARTiSM00360. RRM. 3 hits.
[Graphical view]
SUPFAMiSSF54928. SSF54928. 3 hits.
PROSITEiPS50102. RRM. 3 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiHNRPM_HUMAN
AccessioniPrimary (citable) accession number: P52272
Secondary accession number(s): Q15584
, Q8WZ44, Q96H56, Q9BWL9, Q9Y492
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 23, 2007
Last modified: November 30, 2016
This is version 175 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome, Direct protein sequencing, Reference proteome

Documents

  1. Human chromosome 19
    Human chromosome 19: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  4. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.