Reviewed, UniProtKB/Swiss-Prot P52236 (CCMG_PARDP)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbE homolog
Alternative name(s):
    Cytochrome c biogenesis protein ccmG

Gene names

Name:	ccmG
Ordered Locus Names:	Pden_1410

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	179 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins. Also act as a disulfide oxidoreductase in cytochromes c biogenesis. The cysteines of apocytochromes c must be in the reduced state for covalent linkage between the two moieties to occur.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the thioredoxin family. DsbE subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 25	25	Potential
	Chain	26 – 179	154	Thiol:disulfide interchange protein dsbE homolog		PRO_0000034285
Regions
	Domain	37 – 174	138	Thioredoxin
Amino acid modifications
	Disulfide bond	77 ↔ 80		Redox-active By similarity
Experimental info
	Sequence conflict	28 – 29	2	RD → PN in CAA96497. Ref.1
	Sequence conflict	61 – 62	2	ML → IV in CAA96497. Ref.1
	Sequence conflict	145 – 146	2	ID → MH in CAA96497. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52236-1 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: DC802F6D5ADFA400	FASTA	179	19,361
	10 20 30 40 50 60 MARFSPMMLL PVAIFAGFAG LSGWALLRDD PDALPSAMIG REAPSVGEAT LPGKVQLTDE 70 80 90 100 110 120 MLRQPGVKLV NFWASWCPPC RAEHPTLTEL SARLPVYGVD LKDPEGAALG FLSEHGDPFH 130 140 150 160 170 ALAADPRGRV AIDWGVTAPP ETFIIDGSGR ILHRHAGPLV REDYTNRFLP ELEKALAAE

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Paracoccus denitrificans CcmG is a periplasmic protein-disulphide oxidoreductase required for c- and aa3-type cytochrome biogenesis; evidence for a reductase role in vivo." Page M.D., Ferguson S.J. Mol. Microbiol. 24:977-990(1997) [PubMed: 9220005] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	Z71971 Genomic DNA. Translation: CAA96497.1. CP000489 Genomic DNA. Translation: ABL69511.1. Different initiation.
RefSeq	YP_915207.1.
3D structure databases
HSSP	HSSP built from PDB template 1KNG based on UniProtKB P30960.
ModBase	Search...
Genome annotation databases
GeneID	4581275.
GenomeReviews	Gene locus Pden_1410 in contig CP000489_GR.
KEGG	pde:Pden_1410.
Organism-specific databases
CMR	Search...
Family and domain databases
InterPro	IPR004799. periplasmic_diS_OxRdtase_DsbE. IPR013740. Redoxin. IPR017936. Thioredoxin-like. IPR015467. Thioredoxin_core. IPR017937. Thioredoxin_CS. IPR012335. Thioredoxin_fold. [Graphical view]
Gene3D	G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PANTHER	PTHR10438. Trx. 1 hit.
Pfam	PF08534. Redoxin. 1 hit. [Graphical view]
ProDom	PD003679. Thioredoxin_like. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00385. dsbE. 1 hit.
PROSITE	PS00194. THIOREDOXIN_1. 1 hit. PS51352. THIOREDOXIN_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCMG_PARDP

Accession

Primary (citable) accession number: P52236
Secondary accession number(s): A1B1W7

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 6, 2007
Last modified:	June 16, 2009
This is version 56 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)