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Reviewed, UniProtKB/Swiss-Prot P52235 (DSBA_SHIFL)

Last modified July 7, 2009. Version 66. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data | Customize display text xml rdf/xml gff fasta
Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents

Names and origin

Protein namesRecommended name:
    Thiol:disulfide interchange protein dsbA
Gene names
Name: dsbA
Ordered Locus Names: SF3931, S3816
OrganismShigella flexneri [Complete proteome] [HAMAP]
Taxonomic identifier623 [NCBI]
Taxonomic lineageBacteriaProteobacteriaGammaproteobacteriaEnterobacterialesEnterobacteriaceaeShigella

Protein attributes

Sequence length208 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at protein level.

General annotation (Comments)

Function

Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.

Subcellular location

Periplasm.

Sequence similarities

Belongs to the thioredoxin family. DsbA subfamily.

Contains 1 thioredoxin domain.

Ontologies

Keywords
   Cellular componentPeriplasm
   DomainRedox-active center
Signal
   PTMDisulfide bond
   Technical term3D-structure
Complete proteome
Gene Ontology (GO)
   Biological processcell redox homeostasis

Inferred from electronic annotation. Source: InterPro

   Cellular componentouter membrane-bounded periplasmic space

Inferred from electronic annotation. Source: InterPro

   Molecular functionprotein disulfide oxidoreductase activity

Inferred from electronic annotation. Source: InterPro

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Signal peptide1 – 1919 By similarity
Chain20 – 208189Thiol:disulfide interchange protein dsbA
PRO_0000034267

Regions

Domain20 – 150131Thioredoxin

Amino acid modifications

Disulfide bond49 ↔ 52Redox-active By similarity

Experimental info

Sequence conflict681K → I in BAA07407. Ref.1
Sequence conflict981A → V in BAA07407. Ref.1
Sequence conflict1341A → E in BAA07407. Ref.1
Sequence conflict1611A → E in BAA07407. Ref.1
Sequence conflict183 – 1853QGM → KGL in BAA07407. Ref.1
Sequence conflict2071E → K in BAA07407. Ref.1

Sequences

Sequence LengthMass (Da)Tools
P52235-1 [UniParc].

Last modified January 10, 2003. Version 2.
Checksum: 105A5E876FCAEE55

FASTA20823,105
        10         20         30         40         50         60 
MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP HCYQFEEVLH 

        70         80         90        100        110        120 
ISDNVKKKLP EGVKMTKYHV NFMGGDLGKD LTQAWAVAMA LGVEDKVTVP LFEGVQKTQT 

       130        140        150        160        170        180 
IRSASDIRDV FINAGIKGEE YDAAWNSFVV KSLVAQQEKA AADVQLRGVP AMFVNGKYQL 

       190        200 
NPQGMDTSNM DVFVQQYADT VKYLSEEK 

« Hide

References

« Hide 'large scale' references
[1]"Disulfide oxidoreductase activity of Shigella flexneri is required for release of Ipa proteins and invasion of epithelial cells."
Watarai M., Tobe T., Yoshikawa M., Sasakawa C.
Proc. Natl. Acad. Sci. U.S.A. 92:4927-4931(1995) [PubMed: 7761426] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
Strain: YSH6000 / Serotype 2a.
[2]"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157."
Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. expand/collapse author list , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J.
Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: 301 / Serotype 2a.
[3]"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T."
Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R.
Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

D38253 Genomic DNA. Translation: BAA07407.1.
AE005674 Genomic DNA. Translation: AAN45366.1.
AE014073 Genomic DNA. Translation: AAP18832.1.
RefSeqNP_709659.1.
NP_839021.1.

3D structure databases

EntryMethodResolution (Å)ChainPositionsPDBsum
3DKSX-ray1.90A/B/C/D20-208[»]
SMRP52235. Positions 20-207.
ModBaseSearch...

Genome annotation databases

GeneID1023438.
1080031.
GenomeReviewsGene locus SF3931 in contig AE005674_GR.
Gene locus S3816 in contig AE014073_GR.
KEGGsfl:SF3931.
sfx:S3816.

Organism-specific databases

CMRSearch...

Phylogenomic databases

HOGENOMP52235.
OMAP52235. EGVHYEV.

Enzyme and pathway databases

BioCycSFLE198214:AAN45366.1-MON.

Family and domain databases

InterProIPR001853. OxRdtase_DSBA.
IPR017936. Thioredoxin-like.
IPR017937. Thioredoxin_CS.
IPR012335. Thioredoxin_fold.
[Graphical view]
Gene3DG3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.
PfamPF01323. DSBA. 1 hit.
[Graphical view]
PROSITEPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDSBA_SHIFL
AccessionPrimary (citable) accession number: P52235
Entry history
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 10, 2003
Last modified: July 7, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation projectHAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)

Relevant documents

PDB cross-references

Index of Protein Data Bank (PDB) cross-references

SIMILARITY comments

Index of protein domains and families

Names and origin · Protein attributes · General annotation (Comments) · Ontologies · Sequence annotation (Features) · Sequences · References · Cross-references · Entry information · Relevant documents