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Protein

Thiol:disulfide interchange protein DsbA

Gene

dsbA

Organism
Shigella flexneri
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Names & Taxonomyi

Protein namesi
Recommended name:
Thiol:disulfide interchange protein DsbA
Gene namesi
Name:dsbA
Ordered Locus Names:SF3931, S3816
OrganismiShigella flexneri
Taxonomic identifieri623 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaEnterobacteralesEnterobacteriaceaeShigella
Proteomesi
  • UP000002673 Componenti: Chromosome
  • UP000001006 Componenti: Chromosome

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Periplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Signal peptidei1 – 19By similarityAdd BLAST19
ChainiPRO_000003426720 – 208Thiol:disulfide interchange protein DsbAAdd BLAST189

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Disulfide bondi49 ↔ 52Redox-activePROSITE-ProRule annotation

Keywords - PTMi

Disulfide bond

Proteomic databases

PaxDbiP52235.

Interactioni

Protein-protein interaction databases

STRINGi198214.SF3931.

Structurei

Secondary structure

1208
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Turni25 – 27Combined sources3
Beta strandi28 – 30Combined sources3
Beta strandi40 – 45Combined sources6
Helixi50 – 57Combined sources8
Helixi61 – 68Combined sources8
Beta strandi75 – 79Combined sources5
Beta strandi81 – 84Combined sources4
Helixi85 – 101Combined sources17
Helixi104 – 116Combined sources13
Helixi124 – 133Combined sources10
Helixi138 – 145Combined sources8
Helixi148 – 163Combined sources16
Beta strandi168 – 174Combined sources7
Turni175 – 177Combined sources3
Beta strandi178 – 180Combined sources3
Helixi182 – 184Combined sources3
Helixi190 – 205Combined sources16

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DKSX-ray1.90A/B/C/D20-208[»]
ProteinModelPortaliP52235.
SMRiP52235.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52235.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini20 – 150ThioredoxinPROSITE-ProRule annotationAdd BLAST131

Sequence similaritiesi

Belongs to the thioredoxin family. DsbA subfamily.Curated
Contains 1 thioredoxin domain.PROSITE-ProRule annotation

Keywords - Domaini

Redox-active center, Signal

Phylogenomic databases

eggNOGiENOG4108Z33. Bacteria.
COG0526. LUCA.
HOGENOMiHOG000265316.
KOiK03673.
OMAiDKVSPLM.

Family and domain databases

CDDicd03019. DsbA_DsbA. 1 hit.
Gene3Di3.40.30.10. 2 hits.
InterProiIPR001853. DSBA-like_thioredoxin_dom.
IPR023205. DsbA/DsbL.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF01323. DSBA. 1 hit.
[Graphical view]
PIRSFiPIRSF001488. Tdi_protein. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

P52235-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP
60 70 80 90 100
HCYQFEEVLH ISDNVKKKLP EGVKMTKYHV NFMGGDLGKD LTQAWAVAMA
110 120 130 140 150
LGVEDKVTVP LFEGVQKTQT IRSASDIRDV FINAGIKGEE YDAAWNSFVV
160 170 180 190 200
KSLVAQQEKA AADVQLRGVP AMFVNGKYQL NPQGMDTSNM DVFVQQYADT

VKYLSEEK
Length:208
Mass (Da):23,105
Last modified:January 10, 2003 - v2
Checksum:i105A5E876FCAEE55
GO

Experimental Info

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sequence conflicti68K → I in BAA07407 (PubMed:7761426).Curated1
Sequence conflicti98A → V in BAA07407 (PubMed:7761426).Curated1
Sequence conflicti134A → E in BAA07407 (PubMed:7761426).Curated1
Sequence conflicti161A → E in BAA07407 (PubMed:7761426).Curated1
Sequence conflicti183 – 185QGM → KGL in BAA07407 (PubMed:7761426).Curated3
Sequence conflicti207E → K in BAA07407 (PubMed:7761426).Curated1

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38253 Genomic DNA. Translation: BAA07407.1.
AE005674 Genomic DNA. Translation: AAN45366.1.
AE014073 Genomic DNA. Translation: AAP18832.1.
RefSeqiNP_709659.1. NC_004337.2.
WP_000725335.1. NZ_LVJC01000067.1.

Genome annotation databases

EnsemblBacteriaiAAN45366; AAN45366; SF3931.
AAP18832; AAP18832; S3816.
GeneIDi1023438.
KEGGisfl:SF3931.
sft:NCTC1_04252.
sfx:S3816.
PATRICi18709501. VBIShiFle31049_4175.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
D38253 Genomic DNA. Translation: BAA07407.1.
AE005674 Genomic DNA. Translation: AAN45366.1.
AE014073 Genomic DNA. Translation: AAP18832.1.
RefSeqiNP_709659.1. NC_004337.2.
WP_000725335.1. NZ_LVJC01000067.1.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
3DKSX-ray1.90A/B/C/D20-208[»]
ProteinModelPortaliP52235.
SMRiP52235.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi198214.SF3931.

Proteomic databases

PaxDbiP52235.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiAAN45366; AAN45366; SF3931.
AAP18832; AAP18832; S3816.
GeneIDi1023438.
KEGGisfl:SF3931.
sft:NCTC1_04252.
sfx:S3816.
PATRICi18709501. VBIShiFle31049_4175.

Phylogenomic databases

eggNOGiENOG4108Z33. Bacteria.
COG0526. LUCA.
HOGENOMiHOG000265316.
KOiK03673.
OMAiDKVSPLM.

Miscellaneous databases

EvolutionaryTraceiP52235.

Family and domain databases

CDDicd03019. DsbA_DsbA. 1 hit.
Gene3Di3.40.30.10. 2 hits.
InterProiIPR001853. DSBA-like_thioredoxin_dom.
IPR023205. DsbA/DsbL.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
IPR013766. Thioredoxin_domain.
[Graphical view]
PfamiPF01323. DSBA. 1 hit.
[Graphical view]
PIRSFiPIRSF001488. Tdi_protein. 1 hit.
SUPFAMiSSF52833. SSF52833. 1 hit.
PROSITEiPS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiDSBA_SHIFL
AccessioniPrimary (citable) accession number: P52235
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: January 10, 2003
Last modified: November 2, 2016
This is version 112 of the entry and version 2 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Complete proteome

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.