P52235 (DSBA_SHIFL) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiol:disulfide interchange protein DsbA

Gene names

Name:	dsbA
Ordered Locus Names:	SF3931, S3816

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins such as PhoA or OmpA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by DsbB. It is required for pilus biogenesis.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	periplasmic space Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

Feature key

Position(s)

Length

Description

Graphical view

Feature identifier

Molecule processing

Signal peptide

1 – 19

19

By similarity

Chain

20 – 208

189

Thiol:disulfide interchange protein DsbA

PRO_0000034267

Regions

Domain

20 – 150

131

Thioredoxin

Amino acid modifications

Disulfide bond

49 ↔ 52

Redox-active By similarity

Experimental info

Sequence conflict

68

1

K → I in BAA07407. Ref.1

Sequence conflict

98

1

A → V in BAA07407. Ref.1

Sequence conflict

134

1

A → E in BAA07407. Ref.1

Sequence conflict

161

1

A → E in BAA07407. Ref.1

Sequence conflict

183 – 185

3

QGM → KGL in BAA07407. Ref.1

Sequence conflict

207

1

E → K in BAA07407. Ref.1

Secondary structure

1

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

.

208

Helix Strand Turn

Details...

Beta strand

28 – 30

3

Beta strand

40 – 45

6

Helix

50 – 57

8

Helix

61 – 68

8

Beta strand

75 – 79

5

Helix

90 – 100

11

Helix

104 – 112

9

Helix

124 – 133

10

Helix

140 – 145

6

Helix

148 – 163

16

Beta strand

171 – 174

4

Beta strand

178 – 180

3

Helix

190 – 205

16

Sequences

	Sequence		Length	Mass (Da)
	P52235 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: 105A5E876FCAEE55	FASTA	208	23,105
	10 20 30 40 50 60 MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP HCYQFEEVLH 70 80 90 100 110 120 ISDNVKKKLP EGVKMTKYHV NFMGGDLGKD LTQAWAVAMA LGVEDKVTVP LFEGVQKTQT 130 140 150 160 170 180 IRSASDIRDV FINAGIKGEE YDAAWNSFVV KSLVAQQEKA AADVQLRGVP AMFVNGKYQL 190 200 NPQGMDTSNM DVFVQQYADT VKYLSEEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disulfide oxidoreductase activity of Shigella flexneri is required for release of Ipa proteins and invasion of epithelial cells." Watarai M., Tobe T., Yoshikawa M., Sasakawa C. Proc. Natl. Acad. Sci. U.S.A. 92:4927-4931(1995) [PubMed: 7761426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YSH6000 / Serotype 2a.
[2]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[3]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.
+	Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ

D38253 Genomic DNA. Translation: BAA07407.1.
AE005674 Genomic DNA. Translation: AAN45366.1.
AE014073 Genomic DNA. Translation: AAP18832.1.

RefSeq

NP_709659.1. NC_004337.2.
NP_839021.1. NC_004741.1.

3D structure databases

PDBe
RCSB PDB
PDBj

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DKS	X-ray	1.90	A/B/C/D	20-208	[»]

ProteinModelPortal

P52235.

SMR

P52235. Positions 20-208.

ModBase

Search...

Protocols and materials databases

StructuralBiologyKnowledgebase

Search...

Genome annotation databases

EnsemblBacteria

EBESCT00000086072; EBESCP00000082894; EBESCG00000085117.
EBESCT00000091984; EBESCP00000088620; EBESCG00000091028.

GeneID

1023438.
1080031.

GenomeReviews

Gene locus SF3931 in contig AE005674_GR.
Gene locus S3816 in contig AE014073_GR.

KEGG

sfl:SF3931.
sfx:S3816.

PATRIC

18709501. VBIShiFle31049_4175.

Organism-specific databases

CMR

Search...

Phylogenomic databases

GeneTree

EBGT00050000010220.

HOGENOM

HBG680642.

OMA

MFEAVQK.

ProtClustDB

PRK10954.

Enzyme and pathway databases

BioCyc

SFLE198214:AAN45366.1-MONOMER.

Family and domain databases

InterPro

IPR001853. DSBA-like_thioredoxin_dom.
IPR023205. Thiol:disulphide_interchange.
IPR012336. Thioredoxin-like_fold.
IPR017937. Thioredoxin_CS.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 2 hits.

KO

K03673.

Pfam

PF01323. DSBA. 1 hit.
[Graphical view]

PIRSF

PIRSF001488. Tdi_protein. 1 hit.

SUPFAM

SSF52833. Thiordxn-like_fd. 1 hit.

PROSITE

PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DSBA_SHIFL

Accession

Primary (citable) accession number: P52235

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 10, 2003
Last modified:	January 25, 2012
This is version 84 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program