Reviewed, UniProtKB/Swiss-Prot P52235 (DSBA_SHIFL)

Names and origin

Protein names

Recommended name:
    Thiol:disulfide interchange protein dsbA

Gene names

Name:	dsbA
Ordered Locus Names:	SF3931, S3816

Organism

Shigella flexneri [Complete proteome] [HAMAP]

Taxonomic identifier

623 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Enterobacteriales › Enterobacteriaceae › Shigella

Protein attributes

Sequence length	208 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for disulfide bond formation in some periplasmic proteins such as phoA or ompA. Acts by transferring its disulfide bond to other proteins and is reduced in the process. DsbA is reoxidized by dsbB. It is required for pilus biogenesis.
Subcellular location	Periplasm.
Sequence similarities	Belongs to the thioredoxin family. DsbA subfamily. Contains 1 thioredoxin domain.

Ontologies

Keywords
Cellular component	Periplasm
Domain	Redox-active center Signal
PTM	Disulfide bond
Technical term	3D-structure Complete proteome
Gene Ontology (GO)
Biological process	cell redox homeostasis Inferred from electronic annotation. Source: InterPro
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro
Molecular function	protein disulfide oxidoreductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Signal peptide	1 – 19	19	By similarity
	Chain	20 – 208	189	Thiol:disulfide interchange protein dsbA		PRO_0000034267
Regions
	Domain	20 – 150	131	Thioredoxin
Amino acid modifications
	Disulfide bond	49 ↔ 52		Redox-active By similarity
Experimental info
	Sequence conflict	68	1	K → I in BAA07407. Ref.1
	Sequence conflict	98	1	A → V in BAA07407. Ref.1
	Sequence conflict	134	1	A → E in BAA07407. Ref.1
	Sequence conflict	161	1	A → E in BAA07407. Ref.1
	Sequence conflict	183 – 185	3	QGM → KGL in BAA07407. Ref.1
	Sequence conflict	207	1	E → K in BAA07407. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52235-1 [UniParc]. Last modified January 10, 2003. Version 2. Checksum: 105A5E876FCAEE55	FASTA	208	23,105
	10 20 30 40 50 60 MKKIWLALAG LVLAFSASAA QYEDGKQYTT LEKPVAGAPQ VLEFFSFFCP HCYQFEEVLH 70 80 90 100 110 120 ISDNVKKKLP EGVKMTKYHV NFMGGDLGKD LTQAWAVAMA LGVEDKVTVP LFEGVQKTQT 130 140 150 160 170 180 IRSASDIRDV FINAGIKGEE YDAAWNSFVV KSLVAQQEKA AADVQLRGVP AMFVNGKYQL 190 200 NPQGMDTSNM DVFVQQYADT VKYLSEEK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Disulfide oxidoreductase activity of Shigella flexneri is required for release of Ipa proteins and invasion of epithelial cells." Watarai M., Tobe T., Yoshikawa M., Sasakawa C. Proc. Natl. Acad. Sci. U.S.A. 92:4927-4931(1995) [PubMed: 7761426] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: YSH6000 / Serotype 2a.
[2]	"Genome sequence of Shigella flexneri 2a: insights into pathogenicity through comparison with genomes of Escherichia coli K12 and O157." Jin Q., Yuan Z., Xu J., Wang Y., Shen Y., Lu W., Wang J., Liu H., Yang J., Yang F., Zhang X., Zhang J., Yang G., Wu H., Qu D., Dong J., Sun L., Xue Y. , Zhao A., Gao Y., Zhu J., Kan B., Ding K., Chen S., Cheng H., Yao Z., He B., Chen R., Ma D., Qiang B., Wen Y., Hou Y., Yu J. Nucleic Acids Res. 30:4432-4441(2002) [PubMed: 12384590] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: 301 / Serotype 2a.
[3]	"Complete genome sequence and comparative genomics of Shigella flexneri serotype 2a strain 2457T." Wei J., Goldberg M.B., Burland V., Venkatesan M.M., Deng W., Fournier G., Mayhew G.F., Plunkett G. III, Rose D.J., Darling A., Mau B., Perna N.T., Payne S.M., Runyen-Janecky L.J., Zhou S., Schwartz D.C., Blattner F.R. Infect. Immun. 71:2775-2786(2003) [PubMed: 12704152] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: ATCC 700930 / 2457T / Serotype 2a.

Cross-references

Sequence databases

EMBLGenBankDDBJ

D38253 Genomic DNA. Translation: BAA07407.1.
AE005674 Genomic DNA. Translation: AAN45366.1.
AE014073 Genomic DNA. Translation: AAP18832.1.

RefSeq

NP_709659.1.
NP_839021.1.

3D structure databases

PDBPDBe

Entry	Method	Resolution (Å)	Chain	Positions	PDBsum
3DKS	X-ray	1.90	A/B/C/D	20-208	[»]

SMR

P52235. Positions 20-207.

ModBase

Search...

Genome annotation databases

GeneID

1023438.
1080031.

GenomeReviews

Gene locus SF3931 in contig AE005674_GR.
Gene locus S3816 in contig AE014073_GR.

KEGG

sfl:SF3931.
sfx:S3816.

Organism-specific databases

CMR

Search...

Phylogenomic databases

HOGENOM

P52235.

OMA

P52235. EGVHYEV.

Enzyme and pathway databases

BioCyc

SFLE198214:AAN45366.1-MON.

Family and domain databases

InterPro

IPR001853. OxRdtase_DSBA.
IPR017936. Thioredoxin-like.
IPR017937. Thioredoxin_CS.
IPR012335. Thioredoxin_fold.
[Graphical view]

Gene3D

G3DSA:3.40.30.10. Thioredoxin_fold. 1 hit.

Pfam

PF01323. DSBA. 1 hit.
[Graphical view]

PROSITE

PS00194. THIOREDOXIN_1. 1 hit.
PS51352. THIOREDOXIN_2. 1 hit.
[Graphical view]

ProtoNet

Search...

Entry information

Entry name

DSBA_SHIFL

Accession

Primary (citable) accession number: P52235

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	January 10, 2003
Last modified:	July 7, 2009
This is version 66 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)