P52218 (CCMA_PARDP) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Cytochrome c biogenesis ATP-binding export protein CcmA
EC=3.6.3.41

Alternative name(s):
Heme exporter protein A

Gene names

Name:	ccmA
Ordered Locus Names:	Pden_1413

Organism

Paracoccus denitrificans (strain Pd 1222) [Complete proteome] [HAMAP]

Taxonomic identifier

318586 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Alphaproteobacteria › Rhodobacterales › Rhodobacteraceae › Paracoccus

Protein attributes

Sequence length	210 AA.
Sequence status	Complete.
Protein existence	Evidence at protein level

General annotation (Comments)

Function	Part of the ABC transporter complex CcmAB involved in the biogenesis of c-type cytochromes; once thought to export heme, this seems not to be the case, but its exact role is uncertain. Responsible for energy coupling to the transport system By similarity. HAMAP MF_01707
Catalytic activity	ATP + H2O + heme(In) = ADP + phosphate + heme(Out). HAMAP MF_01707
Subunit structure	The complex is composed of two ATP-binding proteins (CcmA) and two transmembrane proteins (CcmB). Ref.3
Subcellular location	Cell inner membrane; Peripheral membrane protein By similarity HAMAP MF_01707.
Sequence similarities	Belongs to the ABC transporter superfamily. CcmA exporter (TC 3.A.1.107) family. [View classification] Contains 1 ABC transporter domain.

Ontologies

Keywords
Biological process	Cytochrome c-type biogenesis Transport
Cellular component	Cell inner membrane Cell membrane Membrane
Ligand	ATP-binding Nucleotide-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cytochrome complex assembly Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	outer membrane-bounded periplasmic space Inferred from electronic annotation. Source: InterPro plasma membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW heme-transporting ATPase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 210	210	Cytochrome c biogenesis ATP-binding export protein CcmA HAMAP MF_01707		PRO_0000092192
Regions
	Domain	4 – 207	204	ABC transporter
	Nucleotide binding	36 – 43	8	ATP Potential
Experimental info
	Sequence conflict	34	1	L → V in CAA96502. Ref.1
	Sequence conflict	128	1	L → V in CAA96502. Ref.1
	Sequence conflict	153	1	V → GL in CAA96502. Ref.1
	Sequence conflict	159 – 160	2	ML → IV in CAA96502. Ref.1
	Sequence conflict	198 – 202	5	RQSRP → PAIAA in CAA96502. Ref.1

Sequences

	Sequence		Length	Mass (Da)
	P52218 [UniParc]. Last modified March 6, 2007. Version 2. Checksum: A2039284EB60FBE8	FASTA	210	22,169
	10 20 30 40 50 60 MNLLAVRDLA VARGGLRAVE GVCFNLNAGG ALVLRGPNGI GKTTLLRTLA GLQPLVSGVI 70 80 90 100 110 120 EAAPDAIAYA GHSDGLKPAL TVTENLRFWA EIFGGRNIDA ALEAMNLRDL ANRPAHALSA 130 140 150 160 170 180 GQKRRLGLAR LMVTGRPVWL LDEPTVSLDR DSVALFAAML RAHLGRGGAA VIATHIDLGL 190 200 210 PEAEILELGP FRASELRRQS RPAGFNEAFG

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"The Paracoccus denitrificans ccmA, B and C genes: cloning and sequencing, and analysis of the potential of their products to form a haem or apo-c-type cytochrome transporter." Page D., Pearce D.A., Norris H.A., Ferguson S.J. Microbiology 143:563-576(1997) [PubMed: 9043133] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA].
[2]	"Complete sequence of chromosome 1 of Paracoccus denitrificans PD1222." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S., Munk A.C., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J. , Larimer F., Land M., Hauser L., Kyrpides N., Lykidis A., Spiro S., Richardson D.J., Moir J.W.B., Ferguson S.J., van Spanning R.J.M., Richardson P. Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Pd 1222.
[3]	"Mutational analysis of the Paracoccus denitrificans c-type cytochrome biosynthetic genes ccmABCDG: disruption of ccmC has distinct effects suggesting a role for CcmC independent of CcmAB." Page M.D., Ferguson S.J. Microbiology 145:3047-3057(1999) [PubMed: 10589712] [Abstract] Cited for: SUBUNIT.

Cross-references

Sequence databases
EMBL GenBank DDBJ	Z71971 Genomic DNA. Translation: CAA96502.1. CP000489 Genomic DNA. Translation: ABL69514.1.
RefSeq	YP_915210.1. NC_008686.1.
3D structure databases
ProteinModelPortal	P52218.
ModBase	Search...
Protein-protein interaction databases
STRING	P52218.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	4581278.
GenomeReviews	Gene locus Pden_1413 in contig CP000489_GR.
KEGG	pde:Pden_1413.
PATRIC	22853977. VBIParDen97112_1379.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG4133.
HOGENOM	HBG758042.
OMA	IVENLAC.
PhylomeDB	P52218.
ProtClustDB	PRK13539.
Family and domain databases
HAMAP	MF_01707. ABC_ATPase_CcmA. [Tree]
InterPro	IPR003439. ABC_transporter-like. IPR017871. ABC_transporter_CS. IPR005895. ABC_transptr_haem_export_CcmA. IPR003593. ATPase_AAA+_core. [Graphical view]
KO	K02193.
Pfam	PF00005. ABC_tran. 1 hit. [Graphical view]
SMART	SM00382. AAA. 1 hit. [Graphical view]
TIGRFAMs	TIGR01189. CcmA. 1 hit.
PROSITE	PS00211. ABC_TRANSPORTER_1. 1 hit. PS50893. ABC_TRANSPORTER_2. 1 hit. PS51243. CCMA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CCMA_PARDP

Accession

Primary (citable) accession number: P52218
Secondary accession number(s): A1B1X0

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	March 6, 2007
Last modified:	January 25, 2012
This is version 79 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program