P52217 (GRDB_CLOLI) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glycine reductase complex component B subunit gamma
EC=1.21.4.2

Alternative name(s):
Selenoprotein PB gamma

Gene names

Name:

grdB

Organism

Clostridium litorale (Bacterium W6)

Taxonomic identifier

1557 [NCBI]

Taxonomic lineage

cellular organisms › Bacteria › Firmicutes › Clostridia › Clostridiales › Peptostreptococcaceae

Protein attributes

Sequence length	436 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB-activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated selenoether and the respective amine. By action of component PC, acetyl phosphate is formed, leaving component PA in its oxidized state. Finally component PA becomes reduced by the thioredoxin system to start a new catalytic cycle of reductive deamination.
Catalytic activity	Acetyl phosphate + NH3 + thioredoxin disulfide + H2O = glycine + phosphate + thioredoxin.
Subunit structure	Heterohexamer of two alpha, two beta and two gamma subunits. Component of the glycine reductase complex, together with components A and C. PB is substrate specific.
Sequence similarities	Belongs to the GrdB/GrdF/GrdH family.

Ontologies

Keywords
Coding sequence diversity	Selenocysteine
Molecular function	Oxidoreductase
Gene Ontology (GO)
Cellular_component	glycine reductase complex Inferred from electronic annotation. Source: InterPro
Molecular_function	glycine reductase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 436	436	Glycine reductase complex component B subunit gamma		PRO_0000087598
Sites
	Active site	350	1
Amino acid modifications
	Non-standard residue	350	1	Selenocysteine

Sequences

	Sequence		Length	Mass (Da)
	P52217 [UniParc]. Last modified February 26, 2008. Version 2. Checksum: 997AEDCCCD1F0E89	FASTA	436	46,600
	10 20 30 40 50 60 MGKLRVVHYI NQFFAGIGGE EKADIAPFVA EELPPISQNL DKLLGEDAEV VATVVCGDSF 70 80 90 100 110 120 FGENLETAQA TVLEMVKGAS PDLFIAGPAF NAGRYGVAAG AITKAVQDTL GIPAVTGMYI 130 140 150 160 170 180 ENPGADMYKK SVYVMSTADS AAGMRKSLPA LAKFALKYAK GEEIGSPAEE GYIERGIRVN 190 200 210 220 230 240 GFKEDRGAKR AVAMLVKKLK GEEFVTEYPM PVFDNVVPGR AIVNMSKAKI AIVTSGGIVP 250 260 270 280 290 300 KGNPDRIESS SASKYGKYDI DGVDDLTSEG WETAHGGHDP VYANEDADRV IPVDVLRDME 310 320 330 340 350 360 KEGVIGELHR YFYSTTGNGT AVLSSKQFAK EFTQELMAAG VDAVILTSTU GTCTRCGATM 370 380 390 400 410 420 VKEIERSGIP VVHICTVTPI ALTVGANRIV PAIAIPHPLG DPALSPAEEK ALRRKIVEKS 430 LKALETEIEE QTVFED

References

[1]

"Glycine reductase of Clostridium litorale. Cloning, sequencing, and molecular analysis of the grdAB operon that contains two in-frame TGA codons for selenium incorporation." Kreimer S., Andreesen J.R. Eur. J. Biochem. 234:192-199(1995) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA]. Strain: ATCC 49638 / DSM 5388 / W6.

Cross-references

Sequence databases
EMBL GenBank DDBJ	U24268 Genomic DNA. Translation: AAC43574.2.
PIR	S63988.
3D structure databases
ModBase	Search...
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Family and domain databases
InterPro	IPR010186. Gly_red_sel_B. IPR022787. GRDB. IPR010187. Various_sel_PB. [Graphical view]
Pfam	PF07355. GRDB. 1 hit. [Graphical view]
TIGRFAMs	TIGR01917. gly_red_sel_B. 1 hit. TIGR01918. various_sel_PB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GRDB_CLOLI

Accession

Primary (citable) accession number: P52217

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 1, 1996
Last sequence update:	February 26, 2008
Last modified:	April 3, 2013
This is version 53 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program