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Protein

Thiosulfate sulfurtransferase

Gene

rhdA

Organism
Azotobacter vinelandii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei230Cysteine persulfide intermediate1
Binding sitei235Substrate1

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12548.
BRENDAi2.8.1.1. 49.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate sulfurtransferase (EC:2.8.1.1)
Alternative name(s):
Rhodanese-like protein
Gene namesi
Name:rhdA
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00001394061 – 271Thiosulfate sulfurtransferaseAdd BLAST271

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
iscSP0A6B72EBI-7906952,EBI-550055From a different organism.

Protein-protein interaction databases

IntActiP52197. 1 interactor.
MINTiMINT-1771585.
STRINGi322710.Avin_07450.

Structurei

Secondary structure

1271
Legend: HelixTurnBeta strandPDB Structure known for this area
Show more details
Feature keyPosition(s)DescriptionActionsGraphical viewLength
Helixi2 – 4Combined sources3
Beta strandi9 – 11Combined sources3
Helixi13 – 17Combined sources5
Turni18 – 21Combined sources4
Beta strandi25 – 29Combined sources5
Helixi33 – 38Combined sources6
Helixi49 – 52Combined sources4
Helixi66 – 76Combined sources11
Beta strandi83 – 87Combined sources5
Beta strandi89 – 92Combined sources4
Helixi93 – 104Combined sources12
Beta strandi110 – 113Combined sources4
Helixi116 – 122Combined sources7
Helixi151 – 157Combined sources7
Beta strandi163 – 167Combined sources5
Helixi171 – 174Combined sources4
Beta strandi181 – 183Combined sources3
Helixi195 – 198Combined sources4
Helixi201 – 203Combined sources3
Helixi211 – 217Combined sources7
Beta strandi224 – 229Combined sources6
Beta strandi231 – 234Combined sources4
Helixi235 – 245Combined sources11
Beta strandi251 – 253Combined sources3
Helixi257 – 261Combined sources5

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E0CX-ray1.80A1-271[»]
1H4KX-ray2.05X1-271[»]
1H4MX-ray2.10X1-271[»]
ProteinModelPortaliP52197.
SMRiP52197.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52197.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini21 – 129Rhodanese 1PROSITE-ProRule annotationAdd BLAST109
Domaini159 – 270Rhodanese 2PROSITE-ProRule annotationAdd BLAST112

Domaini

Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 rhodanese domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105KHC. Bacteria.
COG2897. LUCA.

Family and domain databases

Gene3Di3.40.250.10. 2 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 2 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
PROSITEiPS00380. RHODANESE_1. 1 hit.
PS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDFASLPLV IEPADLQARL SAPELILVDL TSAARYAEGH IPGARFVDPK
60 70 80 90 100
RTQLGQPPAP GLQPPREQLE SLFGELGHRP EAVYVVYDDE GGGWAGRFIW
110 120 130 140 150
LLDVIGQQRY HYLNGGLTAW LAEDRPLSRE LPAPAGGPVA LSLHDEPTAS
160 170 180 190 200
RDYLLGRLGA ADLAIWDARS PQEYRGEKVL AAKGGHIPGA VNFEWTAAMD
210 220 230 240 250
PSRALRIRTD IAGRLEELGI TPDKEIVTHC QTHHRSGLTY LIAKALGYPR
260 270
VKGYAGSWGE WGNHPDTPVE L
Length:271
Mass (Da):29,629
Last modified:October 1, 1996 - v1
Checksum:i47E573D2D34EA77C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42346 Genomic DNA. Translation: AAB03239.1.
PIRiS62187.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42346 Genomic DNA. Translation: AAB03239.1.
PIRiS62187.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
PDB entryMethodResolution (Å)ChainPositionsPDBsum
1E0CX-ray1.80A1-271[»]
1H4KX-ray2.05X1-271[»]
1H4MX-ray2.10X1-271[»]
ProteinModelPortaliP52197.
SMRiP52197.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52197. 1 interactor.
MINTiMINT-1771585.
STRINGi322710.Avin_07450.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105KHC. Bacteria.
COG2897. LUCA.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12548.
BRENDAi2.8.1.1. 49.

Miscellaneous databases

EvolutionaryTraceiP52197.

Family and domain databases

Gene3Di3.40.250.10. 2 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 2 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
PROSITEiPS00380. RHODANESE_1. 1 hit.
PS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiTHTR_AZOVI
AccessioniPrimary (citable) accession number: P52197
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 2, 2016
This is version 89 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.