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Protein

Thiosulfate sulfurtransferase

Gene

rhdA

Organism
Azotobacter vinelandii
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Experimental evidence at protein leveli

Functioni

Catalytic activityi

Thiosulfate + cyanide = sulfite + thiocyanate.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei230 – 2301Cysteine persulfide intermediate
Binding sitei235 – 2351Substrate

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Transferase

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12548.
BRENDAi2.8.1.1. 49.

Names & Taxonomyi

Protein namesi
Recommended name:
Thiosulfate sulfurtransferase (EC:2.8.1.1)
Alternative name(s):
Rhodanese-like protein
Gene namesi
Name:rhdA
OrganismiAzotobacter vinelandii
Taxonomic identifieri354 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaPseudomonadalesPseudomonadaceaeAzotobacter

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 271271Thiosulfate sulfurtransferasePRO_0000139406Add
BLAST

Interactioni

Binary interactionsi

WithEntry#Exp.IntActNotes
iscSP0A6B72EBI-7906952,EBI-550055From a different organism.

Protein-protein interaction databases

IntActiP52197. 1 interaction.
MINTiMINT-1771585.
STRINGi322710.Avin_07450.

Structurei

Secondary structure

1
271
Legend: HelixTurnBeta strand
Show more details
Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Helixi2 – 43Combined sources
Beta strandi9 – 113Combined sources
Helixi13 – 175Combined sources
Turni18 – 214Combined sources
Beta strandi25 – 295Combined sources
Helixi33 – 386Combined sources
Helixi49 – 524Combined sources
Helixi66 – 7611Combined sources
Beta strandi83 – 875Combined sources
Beta strandi89 – 924Combined sources
Helixi93 – 10412Combined sources
Beta strandi110 – 1134Combined sources
Helixi116 – 1227Combined sources
Helixi151 – 1577Combined sources
Beta strandi163 – 1675Combined sources
Helixi171 – 1744Combined sources
Beta strandi181 – 1833Combined sources
Helixi195 – 1984Combined sources
Helixi201 – 2033Combined sources
Helixi211 – 2177Combined sources
Beta strandi224 – 2296Combined sources
Beta strandi231 – 2344Combined sources
Helixi235 – 24511Combined sources
Beta strandi251 – 2533Combined sources
Helixi257 – 2615Combined sources

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E0CX-ray1.80A1-271[»]
1H4KX-ray2.05X1-271[»]
1H4MX-ray2.10X1-271[»]
ProteinModelPortaliP52197.
SMRiP52197. Positions 1-271.
ModBaseiSearch...
MobiDBiSearch...

Miscellaneous databases

EvolutionaryTraceiP52197.

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Domaini21 – 129109Rhodanese 1PROSITE-ProRule annotationAdd
BLAST
Domaini159 – 270112Rhodanese 2PROSITE-ProRule annotationAdd
BLAST

Domaini

Contains two rhodanese domains with different primary structures but with near identical secondary structure conformations suggesting a common evolutionary origin. Only the C-terminal rhodanese domain contains the catalytic cysteine residue (By similarity).By similarity

Sequence similaritiesi

Contains 2 rhodanese domains.PROSITE-ProRule annotation

Keywords - Domaini

Repeat

Phylogenomic databases

eggNOGiENOG4105KHC. Bacteria.
COG2897. LUCA.

Family and domain databases

Gene3Di3.40.250.10. 2 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 2 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
PROSITEiPS00380. RHODANESE_1. 1 hit.
PS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 2 hits.
[Graphical view]

Sequencei

Sequence statusi: Complete.

P52197-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDDFASLPLV IEPADLQARL SAPELILVDL TSAARYAEGH IPGARFVDPK
60 70 80 90 100
RTQLGQPPAP GLQPPREQLE SLFGELGHRP EAVYVVYDDE GGGWAGRFIW
110 120 130 140 150
LLDVIGQQRY HYLNGGLTAW LAEDRPLSRE LPAPAGGPVA LSLHDEPTAS
160 170 180 190 200
RDYLLGRLGA ADLAIWDARS PQEYRGEKVL AAKGGHIPGA VNFEWTAAMD
210 220 230 240 250
PSRALRIRTD IAGRLEELGI TPDKEIVTHC QTHHRSGLTY LIAKALGYPR
260 270
VKGYAGSWGE WGNHPDTPVE L
Length:271
Mass (Da):29,629
Last modified:October 1, 1996 - v1
Checksum:i47E573D2D34EA77C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42346 Genomic DNA. Translation: AAB03239.1.
PIRiS62187.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
L42346 Genomic DNA. Translation: AAB03239.1.
PIRiS62187.

3D structure databases

Select the link destinations:
PDBei
RCSB PDBi
PDBji
Links Updated
EntryMethodResolution (Å)ChainPositionsPDBsum
1E0CX-ray1.80A1-271[»]
1H4KX-ray2.05X1-271[»]
1H4MX-ray2.10X1-271[»]
ProteinModelPortaliP52197.
SMRiP52197. Positions 1-271.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

IntActiP52197. 1 interaction.
MINTiMINT-1771585.
STRINGi322710.Avin_07450.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Phylogenomic databases

eggNOGiENOG4105KHC. Bacteria.
COG2897. LUCA.

Enzyme and pathway databases

BioCyciMetaCyc:MONOMER-12548.
BRENDAi2.8.1.1. 49.

Miscellaneous databases

EvolutionaryTraceiP52197.

Family and domain databases

Gene3Di3.40.250.10. 2 hits.
InterProiIPR001763. Rhodanese-like_dom.
IPR001307. Thiosulphate_STrfase_CS.
[Graphical view]
PfamiPF00581. Rhodanese. 2 hits.
[Graphical view]
SMARTiSM00450. RHOD. 2 hits.
[Graphical view]
SUPFAMiSSF52821. SSF52821. 2 hits.
PROSITEiPS00380. RHODANESE_1. 1 hit.
PS00683. RHODANESE_2. 1 hit.
PS50206. RHODANESE_3. 2 hits.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Cloning, sequence analysis and overexpression of the rhodanese gene of Azotobacter vinelandii."
    Colnaghi R., Pagani S., Kennedy C., Drummond M.
    Eur. J. Biochem. 236:240-248(1996) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [GENOMIC DNA], PROTEIN SEQUENCE OF 1-18.
    Strain: OP / UW136.
  2. "The crystal structure of a sulfurtransferase from Azotobacter vinelandii highlights the evolutionary relationship between the rhodanese and phosphatase enzyme families."
    Bordo D., Deriu D., Colnaghi R., Carpen A., Pagani S., Bolognesi M.
    J. Mol. Biol. 298:691-704(2000) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (1.8 ANGSTROMS).
  3. "A persulfurated cysteine promotes active site reactivity in Azotobacter vinelandii Rhodanese."
    Bordo D., Forlani F., Spallarossa A., Colnaghi R., Carpen A., Bolognesi M., Pagani S.
    Biol. Chem. 382:1245-1252(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: X-RAY CRYSTALLOGRAPHY (2.05 ANGSTROMS).

Entry informationi

Entry nameiTHTR_AZOVI
AccessioniPrimary (citable) accession number: P52197
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 1, 1996
Last sequence update: October 1, 1996
Last modified: November 11, 2015
This is version 87 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

3D-structure, Direct protein sequencing

Documents

  1. PDB cross-references
    Index of Protein Data Bank (PDB) cross-references
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.